Study on the process of aerobic - anaerobic coupling for dairy farm sewage

doi:10.6048/j.issn.1001-4330.2024.03.023

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 727-733.DOI: 10.6048/j.issn.1001-4330.2024.03.023

• Plant Protection·Microbes • Previous Articles Next Articles

Study on the process of aerobic - anaerobic coupling for dairy farm sewage

LI Yifan¹(), GONG Jiangping², GAO Yan³(), Zeng Jun³, HUO Xiangdong³, LI Fengming¹, LIU Jiancheng¹()

1. College of Animal Sciences, Xinjiang Agricultural University,Urumqi 830011, China
2. Karamay Ruili Kangyuan Landscape works Limited liability company,Karamay Xinjiang 834000,China
3. Institute of Microbiology Application,Xinjiang Academy of Agricultural Sciences/Xinjiang Special Environmental Microbiology Laboratory,Urumqi 830091, China

Received:2023-07-08 Online:2024-03-20 Published:2024-04-19
Correspondence author: LIU Jiancheng (1982 -),male, from Wuwei, Gansu, associate professor, research direction:Treatment and resource utilization of livestock manure,(E-mail)70964390@qq.comGAO Yan(1979-),female,from Jiangyin, Jiangsu, researcher,research field:Resources and Applied Microbiology,(E-mail)47795411@qq.com.
Supported by:
Major S & T Project of Xinjiang Uygur Region(2020A01001-2);Karamay Technology Innovation Pilot Program(20232023cgzh0013);Xinjiang Agricultural University College Student Innovation Project(dxscx2021097);Project of Fund for Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2023005-6)

好氧-厌氧耦合法处理奶牛场污水工艺分析

李一帆¹(), 宫江平², 高雁³(), 曾军³, 霍向东³, 李凤鸣¹, 刘建成¹()

1.新疆农业大学动物科学学院,乌鲁木齐 830011
2.克拉玛依瑞利康源园林工程有限责任公司,新疆克拉玛依 834000
3.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

通讯作者: 刘建成(1982-),男,甘肃武威人,副教授,研究方向为养殖粪污处理及其资源化利用,(E-mail)70964390@qq.com;高雁(1979-),女,江苏江阴人,研究员,博士,研究方向为资源与应用微生物学,(E-mail)47795411@qq.com
作者简介:李一帆(2000-),男,河南博爱人,研究方向为动物营养,(E-mail)li13999263649@163.com
基金资助:
新疆维吾尔自治区重大科技专项(2020A01001-2);克拉玛依技术创新引导计划项目(20232023cgzh0013);新疆农业大学大学生创新项目(dxscx2021097);农业科技创新稳定支持项目(xjnkywdzc-2023005-6)

Abstract

Abstract:

【Objective】 To screen the microorganism that degrades the dariy manure sewage in the hope of providing the scientific basis for the dairy manure treatment.【Methods】 The microbial strains that were reported using to degrade sewage and determine the values of COD(chemical oxygen demand),BOD₅(biological oxygen demang 5 days),TP(total phosphorus)and pH after processing the dairy farm sewage by microorganisms were selected.【Results】 Three strains of aerobic bacteria were screened from the reported beneficial microorganisms that could be used for the treatment of dairy farm sewage,which were Bacillus subtilis,Bacillus megaterium and Bacillus mucilaginous;There were 3 strains of anaerobes,including Rhodopseudomonas palustris,Pseudomonas fluorescens and Seratia marcescens.The best technological conditions for the treatment of dairy farm sewage by the aerobic - anaerobic coupling method were established:5% inoculum,0.05 m³/h aeration(2 h aeration every 10 h),30℃ and 96 h treatment time.【Conclusion】 The removal rate of COD, BOD₅ and TP in the dairy farm sewage is 91.38%,96.82% and 82.6%, respectively,and the effluent pH is 7.2-7.6,which conforms to the national standard for farmland irrigation water.

Key words: aerobic bacteria; anaerobic bacteria; sewage; coupling mechanism; COD; BOD₅

摘要：

【目的】 筛选降解奶牛场粪污水的微生物,为奶牛场粪污处理提供科学依据。【方法】 选择可用于降解污水的微生物菌株,测定微生物处理奶牛场粪污水后的化学需氧量(COD)、生物需氧量(BOD₅)、总磷(TP)和pH的数值。【结果】 从具有降解污水功能的微生物中,筛选出降解效果较好的3株好氧微生物,分别为枯草芽孢杆菌、巨大芽孢杆菌、胶质芽孢杆菌;3株厌氧微生物,分别为沼泽红假单胞菌、荧光假单胞菌、沙雷氏菌;将6种好氧-厌氧菌耦合处理降解奶牛场粪污水,其降解效率均有不同程度的提升,好氧-厌氧耦合法处理奶牛场粪污水的工艺参数为菌种接种量5%、曝气量0.05 m³/h(每隔10 h曝气2 h)、温度30℃、处理时间96 h。【结论】 奶牛场污水中COD去除率为91.38%、BOD₅去除率为96.82%、TP去除率为82.6%,出水pH值为 7.2~7.6,符合国家农田灌溉水标准。

关键词: 好氧菌, 厌氧菌, 污水, 耦合机制, 化学需氧量, 生物需氧量

CLC Number:

Q939.9

LI Yifan, GONG Jiangping, GAO Yan, Zeng Jun, HUO Xiangdong, LI Fengming, LIU Jiancheng. Study on the process of aerobic - anaerobic coupling for dairy farm sewage[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 727-733.

李一帆, 宫江平, 高雁, 曾军, 霍向东, 李凤鸣, 刘建成. 好氧-厌氧耦合法处理奶牛场污水工艺分析[J]. 新疆农业科学, 2024, 61(3): 727-733.

Figures/Tables 6

References 25

[1]	付国宏. 浅谈畜牧场粪污管理和处理方法[J]. 云南农业, 2019,(9):51-53.
	FU Guohong. Talking about the management and treatment of livestock manure[J]. Yunnan Agriculture, 2019,(9):51-53.
[2]	郑煜, 汪秋婷. 畜牧场污水中COD软测量技术研究[J]. 工业设计, 2016,(3):157,159.
	ZHENG Yu, WANG Qiuting. Research on COD soft sensing technology in livestock farm sewage[J]. Industrial Design, 2016,(3):157,159.
	Vorholt J A. Microbial life in the phyllosphere[J]. Nature Reviews Microbiology, 2016,(3):157,159.
[3]	Vorholt J A. Microbial life in the phyllosphere[J]. Nature Reviews Microbiology, 2012, 10(12):828-840. DOI PMID
[4]	张朋月, 丁京涛, 孟海波, 等. 牛粪水酸化贮存过程中氮形态转化的特性研究[J]. 农业工程学报, 2020, 36(8):212-218.
	ZHANG Pengyue, DING Jingtao, MENG Haibo, et al. Study on the characteristics of nitrogen form transformation during acidification and storage of cow dung water[J]. Transations of the Chinese Society of Agricultural Engineering, 2020, 36(8):212-218.
[5]	Csotonyi J T, Swiderski J, Stackebrandt E, et al. A new environment for aerobic anoxygenic phototrophic bacteria:biological soil crusts[J]. Environmental Microbiology Reports, 2010, 2(5): 651-656. DOI PMID
[6]	李维军, 曹鹏, 李春, 等. 好氧-厌氧耦合法处理番茄酱加工有机污水[J]. 化工学报, 2006, 57(12):2970-2975.
	LI Weijun, CAO Peng, LI Chun, et al. Treatment of organic sewage from tomato sauce processing by aerobic and anaerobic coupling method[J]. Journal of Chemical Industry, 2006, 57(12):2970-2975.
[7]	Kembel S W, O’Connor T K, Arnold H K, et al. Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest[C]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(38): 13715-13720.
[8]	于福秋. 畜牧场污水的处理与利用[J]. 养殖技术顾问, 2014,(6):101.
	YU Fuqiu. Treatment and utilization of livestock farm sewage[J]. Technical Advisor for Animal Husbandry, 2014(6):101.
[9]	方志坚, 颜明娟, 叶美锋. 畜牧场污水固液分离后干物质处理利用[J]. 能源与环境, 2006,(5):68-69.
	FANG Zhijian, YAN Mingjuan, YE Meifeng. Treatment and utilization of dry matter after solid-liquid separation of livestock farm sewage[J]. Energy and Environments, 2006,(5):68-69.
[10]	SU Pin, FENG Tuizi, Zhou Xuguo, et al. Isolation of Rhp-PSP,a member of YER057c/YjgF/UK114 protein family with antiviral properties,from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b[J]. Scientific Reports, 2015, 5:16121. DOI PMID
[11]	耿宝民. 集约化畜牧场粪尿污水的治理[J]. 当代畜牧, 2012,(11):47-48.
	GENG Baomin. Treatment of fecal sewage in intensive livestock farm[J]. Contemporary Animal Husbandry, 2012,(11):47-48.
[12]	李维炯, 倪永珍. EM的研究与应用[J]. 生态学杂志, 1995, 14(5):58-62.
	LI Weijiong, NI Yongzhen. Effective microorganisms research and its application[J]. Chinese Journal of Ecology, 1995, 14(5):58-62.
[13]	ZHAO Kaining, XU Rui, ZHANG Ying, et al. Development of a novel compound microbial agent for degradation of kitchen waste[J]. Brazilian Journal of Microbiology, 2017, 48( 3) : 442-450. DOI PMID
[14]	吴金男, 尹凡, 徐建荣, 等. B'yond菌剂液体发酵条件的优化及对养殖废水处理效果的研究[J]. 绿色科技, 2016,(4):83-84.
	WU Jinnan, YIN Fan, XU Jianrong, et al. Optimization of liquid fermentation conditions of B'ond inoculum and study on treatment effect of aquaculture wastewater[J]. Journal of Green Science and Technology, 2016,(4):83-84.
[15]	卢海凤. 基于光合细菌Z08降解大分子有机物的污水处理及其机制研究[D]. 哈尔滨: 哈尔滨工业大学, 2012.
	LU Haifeng. Research on sewage treatment and its mechanism based on the degradation of macromolecular organics by photosynthetic bacteria Z08[D]. Harbin: Harbin Institute of Technology, 2012.
[16]	孙碧玉, 邵继海, 秦普丰, 等. 养猪发酵床中净水芽孢杆菌的分离及其固体发酵研究[J]. 环境工程, 2014, 32(11):60-63.
	SUN Biyu, SHAO Jihai, QIN Pufeng, et al. Separation and its solid fermentation of a water-cleaning bacillus strain from a swine fermentation bed[J]. Environmental Engineering, 2014, 32(11):60-63.
[17]	郭照辉, 尹红梅, 吴迎奔, 等. 高效畜禽废水降解菌的选育及菌群的构建[J]. 环境科学与技术, 2009, 32(11):72-74,80.
	GUO Zhaohui, YIN Hongmei, WU Yingben, et al. Effective strain foe livestock wastewater degradation:Screening/Culturing and constructing Bacterial consortium[J]. Environmental Science and Technology, 2009, 32(11):72-74,80.
[18]	GB11914-1989.水质化学需氧量的测定重铬酸盐法[S].
	GB11914-1989.Water quality-Determination of the chemical oxygen demand-Dichromate method[S].
[19]	HJ 505-2009.水质五日生化需氧量(BOD₅)的测定-稀释与接种法[S].
	HJ 505-2009.Water quality-determination of biochemical oxygen demand after 5 days(BOD₅) for dilution and seeding method[S].
[20]	GB/T 6437-2018.饲料中总磷的测定分光光度法[S].
	GB/T 6437-2018. Determination of phosphorus in feeds-Spectrophotometry[S].
[21]	李军. 微生物与水处理工程[M]. 北京: 化学工艺出版社, 2002,207.
	LI Jun. Microbiological and water treatment[M]. Beijing: Chemical Technology Press, 2002,207.
[22]	杨智. 养殖场污水处理装置与发酵菌剂的研究[D]. 呼和浩特: 内蒙古农业大学, 2021.
	YANG Zhi. Research on Sewage Treatment Device and Fermentation Bacterial Agent of Farm[D]. Hohhot: Inner Mongolia Agricultural University, 2021.
[23]	李刚, 杨克, 许方程, 等. 微生物菌剂处理规模化养猪场粪污水的研究进展[J]. 浙江农业科学, 2018, 59(11):2115-2117.
	LI Gang, YANG Ke, XU Fangcheng, et al. Research progress on treatment of swine manure wastewater by microbial agents[J]. Journal of Zhejiang Agriculture Sciences, 2018, 59(11):2115-2117.
[24]	扶咏梅, 王博, 尚越, 等. 好氧-厌氧耦合技术处理平顶山市某矿区生活污水[J]. 河南城建学院学报, 2015, 24(2):44-48.
	FU Yongmei, WANG Bo, SHANG Yue, et al. Aerobic- Anaerobic coupling process in a mine of Pingdingshan City sewage[J]. Journal of Henan University of Urban Construction, 2015, 24(2):44-48.
[25]	侯树宇, 张清敏, 多淼, 等. 微生物复合菌制剂在对虾养殖中的应用研究[J]. 农业环境科学学报, 2004, 23(5):904-907.
	HOU Shuyu, ZHANG Qingmin, DUO Miao, et al. Application of a Preparation of Mico-Ecological Probiotics in Cultivation of Prawns[J]. Journal of Agro-Environment Science, 2004, 23(5):904-907.

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
巨大芽孢杆菌Bacillus megaterium	176±52	53.4±9.5	1.33±0.21	7.01±0.28
胶质芽孢杆菌Bacillus mucilaginous	188±47	58.2±10.6	1.18±0.16	7.12±0.14
地衣芽孢杆菌Bacillus licheniformis	208±51	60.3±14.1	1.26±0.09	7.24±0.16
解淀粉芽孢杆菌B Bacillus amyloliquefaciens	212±61	61.2±11.7	1.31±0.14	7.30±0.09

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
巨大芽孢杆菌Bacillus megaterium	176±52	53.4±9.5	1.33±0.21	7.01±0.28
胶质芽孢杆菌Bacillus mucilaginous	188±47	58.2±10.6	1.18±0.16	7.12±0.14
地衣芽孢杆菌Bacillus licheniformis	208±51	60.3±14.1	1.26±0.09	7.24±0.16
解淀粉芽孢杆菌B Bacillus amyloliquefaciens	212±61	61.2±11.7	1.31±0.14	7.30±0.09

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
荧光假单胞菌Pseudomonas fluorescens	175±47	50±13	0.71±0.08	6.82±0.02
沙雷氏菌Seratia marcescens	182±56	55±9	0.87±0.10	7.12±0.23
产酸克雷伯氏菌Klebsiella oxytoca	201±63	62±10	0.62±0.12	6.22±0.14
粪肠球菌Enterococcus faecalis	242±86	59±11	0.92±0.18	7.02±0.05
植物乳杆菌Lactobacillus plantarum	476±141	65±12	1.01±0.22	6.38±0.16
嗜热链球菌Streptococcus thermophilus	538±129	82±17	1.20±0.38	7.07±0.21

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
荧光假单胞菌Pseudomonas fluorescens	175±47	50±13	0.71±0.08	6.82±0.02
沙雷氏菌Seratia marcescens	182±56	55±9	0.87±0.10	7.12±0.23
产酸克雷伯氏菌Klebsiella oxytoca	201±63	62±10	0.62±0.12	6.22±0.14
粪肠球菌Enterococcus faecalis	242±86	59±11	0.92±0.18	7.02±0.05
植物乳杆菌Lactobacillus plantarum	476±141	65±12	1.01±0.22	6.38±0.16
嗜热链球菌Streptococcus thermophilus	538±129	82±17	1.20±0.38	7.07±0.21

菌种 Microorganisms	化学需氧量 COD(mg/L)	生物需氧量 BOD₅(mg/L)	总磷 TP(mg/L)	pH
枯草芽孢杆菌Bacillus subtilis	169±38	52.9±13.4	1.22±0.11	7.19±0.45
沼泽红假单胞菌Rhodopseudomonas palustris	168±22	48±7	0.65±0.14	6.44±0.12
好氧菌+厌氧菌耦合^* Aerobic-anaerobic microbial coupling	162±63	42±4	0.64±0.12	7.22±0.14

Study on the process of aerobic - anaerobic coupling for dairy farm sewage

好氧-厌氧耦合法处理奶牛场污水工艺分析

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 25

Related Articles 7

Recommended Articles 0

Metrics

曝气量 Aeration rate (m³/h)	化学需氧量 COD (mg/L)	生物需氧量 BOD₅ (mg/L)	总磷 TP (mg/L)	pH
0.01	191±36	50±11	0.75±0.16	7.15±0.11
0.03	178±32	47±12	0.70±0.12	7.18±0.12
0.05	162±63	42±4	0.64±0.12	7.22±0.14
0.07	164±18	42±9	0.60±0.10	7.11±0.13
0.10	166±12	42±14	0.61±0.06	7.21±0.14

温度 Temper- ature (℃)	化学需氧量 COD (mg/L)	生物需氧量 BOD₅ (mg/L)	总磷 TP (mg/L)	pH
15	426±76	165±56	2.38±0.72	6.44±0.26
20	242±86	82±28	1.92±0.18	6.82±0.12
25	192±42	66±8	0.84±0.11	7.02±0.13
30	160±36	40±12	0.62±0.18	7.20±0.16

[1]	CAO Zhujun, ZHANG Zhenyu, KANG Ning, ZHAO Qian, HU Hongying. Research of parasitoids of Sphaerolecanium prunastri fonscolombe in the western Tianshan wild fruit forest [J]. Xinjiang Agricultural Sciences, 2024, 61(4): 971-983.
[2]	ZENG Jun, WU Lei, GAO Yan, YANG Hongmei, LIN Qing, HUO Xiangdong. Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus [J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2798-2805.
[3]	ZHANG Haiyan, CHEN Guanghui, ZHANG Xiuying, WANG Yutao. Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes [J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2299-2309.
[4]	YANG Xue-mei, FANG Xue, TIAN Ke-chuan, HUANG Xi-xia, ZHU Hua, Ablat Sulayman , CHEN Hua-feng, HE Jun-min, ZHAO Bing-ru, XU Xin-ming, FU Xue-feng, TIAN Yue-zhen, WU Wei-wei, Hanilazi Tulapu , DU Jian-wen. Tissue Expression and Bioinformatics Predictive Analysis of WNT2 Gene in Subo Merino Sheep during Embryonic Stage [J]. Xinjiang Agricultural Sciences, 2019, 56(12): 2320-2328.
[5]	XI Ou-yan;LI Xiao;ZHENG Fei;DUAN Xing-chen;TANG Xiu-li;HU Hong-ying. DNA Barcoding of Agelastica alni orientalis Baly (Coleoptera: Chrysomelidae) [J]. , 2016, 53(2): 309-316.
[6]	ZHAO Ling;ZHAO Li;LI Qin;REN Jin-long. DNA Barcoding of Nymph Grasshoppers from Xinjiang [J]. , 2015, 52(5): 785-794.
[7]	FANG Hui;ZHANG Hua;YAO Zheng-pei;MA Lin;WANG Ze;JIANG Yuan-yuan;MA Hao. Investigation of the Phylogenetic Relationships of Different Color of Haloxylon ammodendron Fruit Wing Based on ITS2 Sequence [J]. , 2015, 52(10): 1822-1827.