Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 727-733.DOI: 10.6048/j.issn.1001-4330.2024.03.023
• Plant Protection·Microbes • Previous Articles Next Articles
LI Yifan1(), GONG Jiangping2, GAO Yan3(
), Zeng Jun3, HUO Xiangdong3, LI Fengming1, LIU Jiancheng1(
)
Received:
2023-07-08
Online:
2024-03-20
Published:
2024-04-19
Correspondence author:
LIU Jiancheng (1982 -),male, from Wuwei, Gansu, associate professor, research direction:Treatment and resource utilization of livestock manure,(E-mail)Supported by:
李一帆1(), 宫江平2, 高雁3(
), 曾军3, 霍向东3, 李凤鸣1, 刘建成1(
)
通讯作者:
刘建成(1982-),男,甘肃武威人,副教授,研究方向为养殖粪污处理及其资源化利用,(E-mail)作者简介:
李一帆(2000-),男,河南博爱人,研究方向为动物营养,(E-mail)li13999263649@163.com
基金资助:
CLC Number:
LI Yifan, GONG Jiangping, GAO Yan, Zeng Jun, HUO Xiangdong, LI Fengming, LIU Jiancheng. Study on the process of aerobic - anaerobic coupling for dairy farm sewage[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 727-733.
李一帆, 宫江平, 高雁, 曾军, 霍向东, 李凤鸣, 刘建成. 好氧-厌氧耦合法处理奶牛场污水工艺分析[J]. 新疆农业科学, 2024, 61(3): 727-733.
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
枯草芽孢杆菌Bacillus subtilis | 169±38 | 52.9±13.4 | 1.22±0.11 | 7.19±0.45 |
巨大芽孢杆菌Bacillus megaterium | 176±52 | 53.4±9.5 | 1.33±0.21 | 7.01±0.28 |
胶质芽孢杆菌Bacillus mucilaginous | 188±47 | 58.2±10.6 | 1.18±0.16 | 7.12±0.14 |
地衣芽孢杆菌Bacillus licheniformis | 208±51 | 60.3±14.1 | 1.26±0.09 | 7.24±0.16 |
解淀粉芽孢杆菌B Bacillus amyloliquefaciens | 212±61 | 61.2±11.7 | 1.31±0.14 | 7.30±0.09 |
Tab.1 Effect of different aerobic microbial treatment on the degradation of dairy sewage
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
枯草芽孢杆菌Bacillus subtilis | 169±38 | 52.9±13.4 | 1.22±0.11 | 7.19±0.45 |
巨大芽孢杆菌Bacillus megaterium | 176±52 | 53.4±9.5 | 1.33±0.21 | 7.01±0.28 |
胶质芽孢杆菌Bacillus mucilaginous | 188±47 | 58.2±10.6 | 1.18±0.16 | 7.12±0.14 |
地衣芽孢杆菌Bacillus licheniformis | 208±51 | 60.3±14.1 | 1.26±0.09 | 7.24±0.16 |
解淀粉芽孢杆菌B Bacillus amyloliquefaciens | 212±61 | 61.2±11.7 | 1.31±0.14 | 7.30±0.09 |
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
沼泽红假单胞菌Rhodopseudomonas palustris | 168±22 | 48±7 | 0.65±0.14 | 6.44±0.12 |
荧光假单胞菌Pseudomonas fluorescens | 175±47 | 50±13 | 0.71±0.08 | 6.82±0.02 |
沙雷氏菌Seratia marcescens | 182±56 | 55±9 | 0.87±0.10 | 7.12±0.23 |
产酸克雷伯氏菌Klebsiella oxytoca | 201±63 | 62±10 | 0.62±0.12 | 6.22±0.14 |
粪肠球菌Enterococcus faecalis | 242±86 | 59±11 | 0.92±0.18 | 7.02±0.05 |
植物乳杆菌Lactobacillus plantarum | 476±141 | 65±12 | 1.01±0.22 | 6.38±0.16 |
嗜热链球菌Streptococcus thermophilus | 538±129 | 82±17 | 1.20±0.38 | 7.07±0.21 |
Tab.2 Effect of different anaerobic microbial treatment on the degradation of dairy sewage
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
沼泽红假单胞菌Rhodopseudomonas palustris | 168±22 | 48±7 | 0.65±0.14 | 6.44±0.12 |
荧光假单胞菌Pseudomonas fluorescens | 175±47 | 50±13 | 0.71±0.08 | 6.82±0.02 |
沙雷氏菌Seratia marcescens | 182±56 | 55±9 | 0.87±0.10 | 7.12±0.23 |
产酸克雷伯氏菌Klebsiella oxytoca | 201±63 | 62±10 | 0.62±0.12 | 6.22±0.14 |
粪肠球菌Enterococcus faecalis | 242±86 | 59±11 | 0.92±0.18 | 7.02±0.05 |
植物乳杆菌Lactobacillus plantarum | 476±141 | 65±12 | 1.01±0.22 | 6.38±0.16 |
嗜热链球菌Streptococcus thermophilus | 538±129 | 82±17 | 1.20±0.38 | 7.07±0.21 |
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
枯草芽孢杆菌Bacillus subtilis | 169±38 | 52.9±13.4 | 1.22±0.11 | 7.19±0.45 |
沼泽红假单胞菌Rhodopseudomonas palustris | 168±22 | 48±7 | 0.65±0.14 | 6.44±0.12 |
好氧菌+厌氧菌耦合* Aerobic-anaerobic microbial coupling | 162±63 | 42±4 | 0.64±0.12 | 7.22±0.14 |
Tab.3 Effect of aerobic-anaerobic microbial coupling treatment on the degradation of dairy sewage
菌种 Microorganisms | 化学需氧量 COD(mg/L) | 生物需氧量 BOD5(mg/L) | 总磷 TP(mg/L) | pH |
---|---|---|---|---|
枯草芽孢杆菌Bacillus subtilis | 169±38 | 52.9±13.4 | 1.22±0.11 | 7.19±0.45 |
沼泽红假单胞菌Rhodopseudomonas palustris | 168±22 | 48±7 | 0.65±0.14 | 6.44±0.12 |
好氧菌+厌氧菌耦合* Aerobic-anaerobic microbial coupling | 162±63 | 42±4 | 0.64±0.12 | 7.22±0.14 |
曝气量 Aeration rate (m3/h) | 化学 需氧量 COD (mg/L) | 生物 需氧量 BOD5 (mg/L) | 总磷 TP (mg/L) | pH |
---|---|---|---|---|
0.01 | 191±36 | 50±11 | 0.75±0.16 | 7.15±0.11 |
0.03 | 178±32 | 47±12 | 0.70±0.12 | 7.18±0.12 |
0.05 | 162±63 | 42±4 | 0.64±0.12 | 7.22±0.14 |
0.07 | 164±18 | 42±9 | 0.60±0.10 | 7.11±0.13 |
0.10 | 166±12 | 42±14 | 0.61±0.06 | 7.21±0.14 |
Tab.4 Effect of different aeration rate aerobic-anaerobic microbial coupling treatment on the degradation of dairy sewage
曝气量 Aeration rate (m3/h) | 化学 需氧量 COD (mg/L) | 生物 需氧量 BOD5 (mg/L) | 总磷 TP (mg/L) | pH |
---|---|---|---|---|
0.01 | 191±36 | 50±11 | 0.75±0.16 | 7.15±0.11 |
0.03 | 178±32 | 47±12 | 0.70±0.12 | 7.18±0.12 |
0.05 | 162±63 | 42±4 | 0.64±0.12 | 7.22±0.14 |
0.07 | 164±18 | 42±9 | 0.60±0.10 | 7.11±0.13 |
0.10 | 166±12 | 42±14 | 0.61±0.06 | 7.21±0.14 |
温度 Temper- ature (℃) | 化学 需氧量 COD (mg/L) | 生物 需氧量 BOD5 (mg/L) | 总磷 TP (mg/L) | pH |
---|---|---|---|---|
15 | 426±76 | 165±56 | 2.38±0.72 | 6.44±0.26 |
20 | 242±86 | 82±28 | 1.92±0.18 | 6.82±0.12 |
25 | 192±42 | 66±8 | 0.84±0.11 | 7.02±0.13 |
30 | 160±36 | 40±12 | 0.62±0.18 | 7.20±0.16 |
Tab.5 Effect of different temperature aerobic-anaerobic microbial coupling treatment on the degradation of dairy sewage
温度 Temper- ature (℃) | 化学 需氧量 COD (mg/L) | 生物 需氧量 BOD5 (mg/L) | 总磷 TP (mg/L) | pH |
---|---|---|---|---|
15 | 426±76 | 165±56 | 2.38±0.72 | 6.44±0.26 |
20 | 242±86 | 82±28 | 1.92±0.18 | 6.82±0.12 |
25 | 192±42 | 66±8 | 0.84±0.11 | 7.02±0.13 |
30 | 160±36 | 40±12 | 0.62±0.18 | 7.20±0.16 |
[1] | 付国宏. 浅谈畜牧场粪污管理和处理方法[J]. 云南农业, 2019,(9):51-53. |
FU Guohong. Talking about the management and treatment of livestock manure[J]. Yunnan Agriculture, 2019,(9):51-53. | |
[2] | 郑煜, 汪秋婷. 畜牧场污水中COD软测量技术研究[J]. 工业设计, 2016,(3):157,159. |
ZHENG Yu, WANG Qiuting. Research on COD soft sensing technology in livestock farm sewage[J]. Industrial Design, 2016,(3):157,159. | |
Vorholt J A. Microbial life in the phyllosphere[J]. Nature Reviews Microbiology, 2016,(3):157,159. | |
[3] |
Vorholt J A. Microbial life in the phyllosphere[J]. Nature Reviews Microbiology, 2012, 10(12):828-840.
DOI PMID |
[4] | 张朋月, 丁京涛, 孟海波, 等. 牛粪水酸化贮存过程中氮形态转化的特性研究[J]. 农业工程学报, 2020, 36(8):212-218. |
ZHANG Pengyue, DING Jingtao, MENG Haibo, et al. Study on the characteristics of nitrogen form transformation during acidification and storage of cow dung water[J]. Transations of the Chinese Society of Agricultural Engineering, 2020, 36(8):212-218. | |
[5] |
Csotonyi J T, Swiderski J, Stackebrandt E, et al. A new environment for aerobic anoxygenic phototrophic bacteria:biological soil crusts[J]. Environmental Microbiology Reports, 2010, 2(5): 651-656.
DOI PMID |
[6] | 李维军, 曹鹏, 李春, 等. 好氧-厌氧耦合法处理番茄酱加工有机污水[J]. 化工学报, 2006, 57(12):2970-2975. |
LI Weijun, CAO Peng, LI Chun, et al. Treatment of organic sewage from tomato sauce processing by aerobic and anaerobic coupling method[J]. Journal of Chemical Industry, 2006, 57(12):2970-2975. | |
[7] | Kembel S W, O’Connor T K, Arnold H K, et al. Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest[C]. Proceedings of the National Academy of Sciences of the United States of America, 2014, 111(38): 13715-13720. |
[8] | 于福秋. 畜牧场污水的处理与利用[J]. 养殖技术顾问, 2014,(6):101. |
YU Fuqiu. Treatment and utilization of livestock farm sewage[J]. Technical Advisor for Animal Husbandry, 2014(6):101. | |
[9] | 方志坚, 颜明娟, 叶美锋. 畜牧场污水固液分离后干物质处理利用[J]. 能源与环境, 2006,(5):68-69. |
FANG Zhijian, YAN Mingjuan, YE Meifeng. Treatment and utilization of dry matter after solid-liquid separation of livestock farm sewage[J]. Energy and Environments, 2006,(5):68-69. | |
[10] |
SU Pin, FENG Tuizi, Zhou Xuguo, et al. Isolation of Rhp-PSP,a member of YER057c/YjgF/UK114 protein family with antiviral properties,from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b[J]. Scientific Reports, 2015, 5:16121.
DOI PMID |
[11] | 耿宝民. 集约化畜牧场粪尿污水的治理[J]. 当代畜牧, 2012,(11):47-48. |
GENG Baomin. Treatment of fecal sewage in intensive livestock farm[J]. Contemporary Animal Husbandry, 2012,(11):47-48. | |
[12] | 李维炯, 倪永珍. EM的研究与应用[J]. 生态学杂志, 1995, 14(5):58-62. |
LI Weijiong, NI Yongzhen. Effective microorganisms research and its application[J]. Chinese Journal of Ecology, 1995, 14(5):58-62. | |
[13] |
ZHAO Kaining, XU Rui, ZHANG Ying, et al. Development of a novel compound microbial agent for degradation of kitchen waste[J]. Brazilian Journal of Microbiology, 2017, 48( 3) : 442-450.
DOI PMID |
[14] | 吴金男, 尹凡, 徐建荣, 等. B'yond菌剂液体发酵条件的优化及对养殖废水处理效果的研究[J]. 绿色科技, 2016,(4):83-84. |
WU Jinnan, YIN Fan, XU Jianrong, et al. Optimization of liquid fermentation conditions of B'ond inoculum and study on treatment effect of aquaculture wastewater[J]. Journal of Green Science and Technology, 2016,(4):83-84. | |
[15] | 卢海凤. 基于光合细菌Z08降解大分子有机物的污水处理及其机制研究[D]. 哈尔滨: 哈尔滨工业大学, 2012. |
LU Haifeng. Research on sewage treatment and its mechanism based on the degradation of macromolecular organics by photosynthetic bacteria Z08[D]. Harbin: Harbin Institute of Technology, 2012. | |
[16] | 孙碧玉, 邵继海, 秦普丰, 等. 养猪发酵床中净水芽孢杆菌的分离及其固体发酵研究[J]. 环境工程, 2014, 32(11):60-63. |
SUN Biyu, SHAO Jihai, QIN Pufeng, et al. Separation and its solid fermentation of a water-cleaning bacillus strain from a swine fermentation bed[J]. Environmental Engineering, 2014, 32(11):60-63. | |
[17] | 郭照辉, 尹红梅, 吴迎奔, 等. 高效畜禽废水降解菌的选育及菌群的构建[J]. 环境科学与技术, 2009, 32(11):72-74,80. |
GUO Zhaohui, YIN Hongmei, WU Yingben, et al. Effective strain foe livestock wastewater degradation:Screening/Culturing and constructing Bacterial consortium[J]. Environmental Science and Technology, 2009, 32(11):72-74,80. | |
[18] | GB11914-1989.水质化学需氧量的测定重铬酸盐法[S]. |
GB11914-1989.Water quality-Determination of the chemical oxygen demand-Dichromate method[S]. | |
[19] | HJ 505-2009.水质五日生化需氧量(BOD5)的测定-稀释与接种法[S]. |
HJ 505-2009.Water quality-determination of biochemical oxygen demand after 5 days(BOD5) for dilution and seeding method[S]. | |
[20] | GB/T 6437-2018.饲料中总磷的测定分光光度法[S]. |
GB/T 6437-2018. Determination of phosphorus in feeds-Spectrophotometry[S]. | |
[21] | 李军. 微生物与水处理工程[M]. 北京: 化学工艺出版社, 2002,207. |
LI Jun. Microbiological and water treatment[M]. Beijing: Chemical Technology Press, 2002,207. | |
[22] | 杨智. 养殖场污水处理装置与发酵菌剂的研究[D]. 呼和浩特: 内蒙古农业大学, 2021. |
YANG Zhi. Research on Sewage Treatment Device and Fermentation Bacterial Agent of Farm[D]. Hohhot: Inner Mongolia Agricultural University, 2021. | |
[23] | 李刚, 杨克, 许方程, 等. 微生物菌剂处理规模化养猪场粪污水的研究进展[J]. 浙江农业科学, 2018, 59(11):2115-2117. |
LI Gang, YANG Ke, XU Fangcheng, et al. Research progress on treatment of swine manure wastewater by microbial agents[J]. Journal of Zhejiang Agriculture Sciences, 2018, 59(11):2115-2117. | |
[24] | 扶咏梅, 王博, 尚越, 等. 好氧-厌氧耦合技术处理平顶山市某矿区生活污水[J]. 河南城建学院学报, 2015, 24(2):44-48. |
FU Yongmei, WANG Bo, SHANG Yue, et al. Aerobic- Anaerobic coupling process in a mine of Pingdingshan City sewage[J]. Journal of Henan University of Urban Construction, 2015, 24(2):44-48. | |
[25] | 侯树宇, 张清敏, 多淼, 等. 微生物复合菌制剂在对虾养殖中的应用研究[J]. 农业环境科学学报, 2004, 23(5):904-907. |
HOU Shuyu, ZHANG Qingmin, DUO Miao, et al. Application of a Preparation of Mico-Ecological Probiotics in Cultivation of Prawns[J]. Journal of Agro-Environment Science, 2004, 23(5):904-907. |
[1] | CAO Zhujun, ZHANG Zhenyu, KANG Ning, ZHAO Qian, HU Hongying. Research of parasitoids of Sphaerolecanium prunastri fonscolombe in the western Tianshan wild fruit forest [J]. Xinjiang Agricultural Sciences, 2024, 61(4): 971-983. |
[2] | ZENG Jun, WU Lei, GAO Yan, YANG Hongmei, LIN Qing, HUO Xiangdong. Optimization of experimental conditions for the treatment of potato starch wastewater by coupling of acidophilic photosynthetic bacteria and Bacillus atrophaeus [J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2798-2805. |
[3] | ZHANG Haiyan, CHEN Guanghui, ZHANG Xiuying, WANG Yutao. Rapid Identification of Farmland's Cicadellidae in Southern Foothills of Pamirs Plateau in China Based on DNA Bar-coding Genes [J]. Xinjiang Agricultural Sciences, 2020, 57(12): 2299-2309. |
[4] | YANG Xue-mei, FANG Xue, TIAN Ke-chuan, HUANG Xi-xia, ZHU Hua, Ablat Sulayman , CHEN Hua-feng, HE Jun-min, ZHAO Bing-ru, XU Xin-ming, FU Xue-feng, TIAN Yue-zhen, WU Wei-wei, Hanilazi Tulapu , DU Jian-wen. Tissue Expression and Bioinformatics Predictive Analysis of WNT2 Gene in Subo Merino Sheep during Embryonic Stage [J]. Xinjiang Agricultural Sciences, 2019, 56(12): 2320-2328. |
[5] | XI Ou-yan;LI Xiao;ZHENG Fei;DUAN Xing-chen;TANG Xiu-li;HU Hong-ying. DNA Barcoding of Agelastica alni orientalis Baly (Coleoptera: Chrysomelidae) [J]. , 2016, 53(2): 309-316. |
[6] | ZHAO Ling;ZHAO Li;LI Qin;REN Jin-long. DNA Barcoding of Nymph Grasshoppers from Xinjiang [J]. , 2015, 52(5): 785-794. |
[7] | FANG Hui;ZHANG Hua;YAO Zheng-pei;MA Lin;WANG Ze;JIANG Yuan-yuan;MA Hao. Investigation of the Phylogenetic Relationships of Different Color of Haloxylon ammodendron Fruit Wing Based on ITS2 Sequence [J]. , 2015, 52(10): 1822-1827. |
Viewed | ||||||
Full text 30
|
|
|||||
Abstract |
|
|||||