Research of parasitoids of Sphaerolecanium prunastri fonscolombe in the western Tianshan wild fruit forest

doi:10.6048/j.issn.1001-4330.2024.04.022

Abstract

Abstract:

【Objective】 The aim of this study is to identify the parasitoids resources of the important invasive species Sphaerolecanium prunastri Fonscolombe in the wild fruit forests of the Western Tianshan Mountains in Xinjiang in the hope of providing an important basis for the subsequent control of this invasive pest using native parasitoids.【Methods】 In this study, parasitic enemies of S. prunastri were obtained by laboratory rearing of field-collected hosts and were classified using morphological and DNA barcoding methods.【Results】 A total of 7 parasitoids were obtained, among which 3 species (Discodes coccophagus Ratzeburg, Microterys hortulanus Erdös, and Aprostocetus trjapitzini Kostjukov) were new records for China. Additionally, this study provided the barcode sequences of the 28S rDNA and COI genes of the 7 parasitoids and their Genebank accession numbers.【Conclusion】 The Pachyneuron solitarium (Hartig) is the dominant parasitoid on Prunus divaricata Ldb., and M. hortulanus is the dominant parasitoid on Armeniaca vulgaris Lamarck.

Key words: Sphaerolecanium prunastri Fonscolombe; chalcidoidea; new record; DNA barcoding; biological control

摘要：

【目的】研究新疆西天山野果林外来入侵物种杏鬃球蚧Sphaerolecanium prunastri Fonscolombe的寄生蜂资源,为利用本土寄生蜂防治该外来入侵害虫提供重要依据。【方法】野外采集寄主并进行室内饲养,获得杏鬃球蚧寄生性天敌,并运用形态学、DNA条形码鉴定物种。【结果】共获得寄生蜂7种,其中中国新记录3种(食蚧点刻跳小蜂Discodes coccophagus Ratzeburg、园囿花翅跳小蜂Microterys hortulanus Erdös、提亚长尾啮姬小峰Aprostocetus trjapitzini Kostjukov),同时提供了7种寄生蜂的28S rDNA及COI基因序列及其Genebank登录号。【结论】松毛虫楔缘金小蜂Pachyneuron solitarium (Hartig)为野樱桃李Prunus divaricata Ldb上的优势寄生蜂,园囿花翅跳小蜂为野杏树Armeniaca vulgaris Lamarck上的优势寄生蜂。

关键词: 杏鬃球蚧, 小蜂总科, 新记录种, DNA条形码, 生物防治

CLC Number:

CAO Zhujun, ZHANG Zhenyu, KANG Ning, ZHAO Qian, HU Hongying. Research of parasitoids of Sphaerolecanium prunastri fonscolombe in the western Tianshan wild fruit forest[J]. Xinjiang Agricultural Sciences, 2024, 61(4): 971-983.

曹竹君, 张振宇, 康宁, 赵倩, 胡红英. 新疆西天山野果林杏鬃球蚧寄生蜂资源调查[J]. 新疆农业科学, 2024, 61(4): 971-983.

Figures/Tables 11

References 30

[1]	布海丽且姆·阿卜杜热合曼, 刘会良, 刘娜, 等. 天山野果林种子植物区系分析[J]. 干旱区地理, 2016, 39(4):828-834.
	Buhailiqiemu Abudureheman, LIU Hui-liang, LIU Na, et al. Spermatophyte floras in the wild fruit forest in Tianshan Mountains[J]. Arid Land Geography, 2016, 39(4):828-834.
[2]	赵鸿飞, 陈天华, 李春蕾. 新疆天山野果林病虫害预测与预警系统研究[J]. 吉林农业, 2019,(1): 74-76.
	ZHAO Hongfei, CHEN Tianhua, LI Chunlei. Study on the prediction and early warning system of diseases and insect pests in Tianshan Mountain, Xinjiang[J]. Agriculture of Jilin, 2019,(1): 74-76.
[3]	王玉丽, 吕昭智, 令狐伟, 等. 新疆野果林的新害虫——杏树鬃球蚧(半翅目: 蚧总科: 蚧科)[J]. 林业科学研究, 2021, 34(5): 152-158.
	WANG Yuli, LYU Zhaozhi, LING Huwei, et al. Sphaerolecanium prunastri (Hemiptera: Coccoidea: Coccidae), A new pest in wild fruit forests, Xinjiang[J]. Forest Research, 2021, 34(5): 152-158.
[4]	王玉丽, 吕昭智, 令狐伟, 等. 新疆野果林杏树鬃球蚧的发生及危害[J]. 新疆农业科学, 2022, 59(7):1741-1747. DOI
	WANG Yuli, LYU Zhaozhi, LIN Huwei, et al. Occurrence and Harm of Sphaerolecanium prunastri in wild Fruit Forests in Xinjiang[J]. Xinjiang Agricultural Sciences, 2022, 59(7): 1741-1747. DOI
[5]	汤祊德, 郝静钧, 师光禄, 等. 我国盾蚧科一新记录属及三新种(同翅目)[J]. 昆虫学报, 1991, 34(4): 458-464.
	TANG Fangde, HAO Jinjun, SHI Guanglu, et al. On a newly found genus and three new species of Diaspididae from China (homoptera)[J]. Acta Entomologica Sinica, 1991, 34(4): 458-464.
[6]	Fallahzadeh M, Japoshvili G. An updated checklist of Iranian Encyrtidae (Hymenoptera, Chalcidoidea)[J]. Zootaxa, 2017, 4344(1): 1-46. DOI PMID
[7]	Moglan I. Complexes parasitaires de quelques espèces de coccides (Homoptera, Coccidae) en Roumanie[J]. Entomologica Romanica, 2007, 12:267-275.
[8]	Japoshvili G, Ay R, Karaca I, et al. Studies on the parasitoid complex attacking the globose scale Sphaerolecanium prunastri (fonscolombe) (hemiptera: Coccoidea) on Prunus species in Turkey[J]. Journal of the Kansas Entomological Society, 2008, 81(4): 339-344.
[9]	Karaca I, Japoshvili G, Demirozer O. The chalcid parasitoid complex (Hymenoptera: Chalcidoidea) associated with the globose scale (Sphaerolecanium prunastri Fonscolombe) (Hemiptera: Coccoidea) in Isparta Province, Turkey and some East European countries[J]. Journal of Plant Diseases and Protection, 2003, 110(5): 505-511.
[10]	LI Zirao, HUANG Lan, ZHAO Wenxia, et al. A new species of the genus Coccophagus(Hymenoptera: Aphelinidae) associated with Sphaerolecanium prunastri(Hemiptera: Coccoidea) from the Tianshan Mountains, Xinjiang[J]. Entomotaxonomia, 2022, 44(3): 228-239.
[11]	Noyes J S. Universal Chalcidoidea Database. World Wide Web electronic publication[EB/OL]. [2023-03]. http://www.nhm.ac.uk/chalcidoids .
[12]	lgentürk S. Parasitoids and predators of Coccidae (homoptera: Coccoidea) species on ornamental plants in Ankara, turkey[J]. Acta Phytopathologica et Entomologica Hungarica, 2001, 36(3/4): 369-375.
[13]	Yousefi M, Vahedi H A, Ebrahimi E, et al. Identification of parasitoid wasps of scale insects (Chalcidoidea) in fruit orchards of Markazi province[J]. Applied Entomology and Phytopathology, 2018, 85(2):251-264.
[14]	廖定熹, 李学骝, 庞雄飞, 等. 中国经济昆虫志.第三十四册, 膜翅目:小蜂总科[M]. 北京: 科学出版社, 1987.
	LIAO Dingjia, LI Xueliu, PAN Xiongfei, et al. China's economic insect record. Book 34, Hymenoptera: Microfamily[M]. Beijing: Science Press, 1987.
[15]	王玉丽. 伊犁野果林杏树鬃球蚧的生物学特性及优势天敌控害作用研究[D]. 新疆农业大学, 2021.
	WANG Yuli. Biology of Sphaerolecanium prunastri and its control by dominant natural enemy in wild fruit forest in Yili[D]. Urumqi: Xinjiang Agricultural University, 2021.
[16]	Trjapitzin V A. Parasitic Hymenoptera of the Family Encyrtidae of Palaearctics[M]. Russia: Nauka, 1989: 428-594.
[17]	黄建. 中国蚜小蜂科分类-膜翅目, 小蜂总科: 膜翅目小蜂总科[M]. 重庆: 重庆出版社, 1994: 28-29.
	HUANG Jian. Systematic studies on Aphelinidae of China[M]. Chongqing: Chongqing Publishing Group, 1994: 28-29.
[18]	肖晖, 黄大卫, 矫天扬. 中国动物志. 第64卷, 金小蜂科(二)金小蜂亚科[M]. 北京: 科学出版社, 2019: 284-286.
	XIAO Hui, HUANG Dawei, QIAO Tianyang. Animal records of China. Volume 64, Family (2)[M]. Beijing: Science Press, 2019: 284-286.
[19]	徐志宏, 黄建. 中国介壳虫寄生蜂志[M]. 上海: 上海科学技术出版社, 2004.
	XU Zhihong, HUANG Jian. Chinese fauna of parasitic wasps on scale insects[M]. Shanghai: Shanghai Scientific & Technical Publishers, 2004.
[20]	Mercet R G, Fauna ib G, HUANG Jian. Chinese fauna of pa[M]. Museo Nacional de Ciencias Naturales, 1921.
[21]	Silvestri F. Contribuzioni alla conoscenza degli insetti dannosi e dei loro simbionti: IV. La Cocciniglia del Prugno (Sphaerolecanium prunastri Fonsc.)[M]. Bollettino del Laboratorio di Zoologia Generale e Agraria della R.Scuola Superiore d'Agricoltura, Portici, 1919.
[22]	Erdös J. Gezogene und gesammelte neue Zehrwespen aus Ungarn[J]. Acta Agronomica Academiae Scientiarum Hungaricae, 1956, 6:379-381.
[23]	Graham M. A reclassification of the European tetrastichinae (Hymenoptera: Eulophidae), with a revision of certain genera[M]. London: British Museum (Natural History), 1987.
[24]	盛子耀, 李为争, 原国辉. 植物气味多样性与昆虫关系的研究进展[J]. 应用昆虫学报, 2019, 56(4): 652-661.
	SHENG Ziyao, LI Weizheng, YUAN Guohui. Advances in studies on the relationship between plant odor diversity and insects[J]. Chinese Journal of Applied Entomology, 2019, 56(4): 652-661.
[25]	孙仲享, 宋圆圆, 曾任森. 植物挥发物介导的种内与种间关系研究进展[J]. 华南农业大学学报, 2019, 40(5): 166-174.
	SUN Zhongxiang, SONG Yuanyuan, ZENG Rensen. Advances in studies on intraspecific and interspecific relationships mediated by plant volatiles[J]. Journal of South China Agricultural University, 2019, 40(5): 166-174.
[26]	Takabayashi J. Herbivory-induced plant volatiles mediate multitrophic relationships in ecosystems[J]. Plant & Cell Physiology, 2022, 63(10): 1344-1355.
[27]	MENG Ziye, LEI Olianglei, CHEN Xiaoqin, et al. Phylogenetic analysis of click beetles(Coleoptera: Elateridae) based upon 28S rDNA: phylogeny and classification[J]. Entomotaxonomia, 2018, 40(3): 231-252.
[28]	武宇鹏, 范仁俊, 曹天文, 等. 北京白羊沟景区农林交错带蛾类DNA条形码鉴定[J]. 应用昆虫学报, 2021, 58(2): 347-363.
	WU Yupeng, FAN Renjun, CAO Tianwen, et al. Using DNA barcoding to classify moths in an agroforestry zone in Baiyanggou district, Beijing, China[J]. Chinese Journal of Applied Entomology, 2021, 58(2): 347-363.
[29]	张慧. 黄蚜小蜂属部分种类的形态鉴别及分子系统发育研究[D]. 福州: 福建农林大学, 2016.
	ZHANG Hui. Morphological Identification and Molecular Phylogeny of Some Species of Aphytis (Aphelinidae: Hymenoptera)[D]. Fuzhou: Fujian Agriculture and Forestry University, 2016.
[30]	邵天玉, 刘思竹, 谢维欣, 等. DNA条形码技术在黑龙江地区姬小蜂科物种鉴定中的应用与分析[J]. 安徽农业科学, 2023, 51(5): 78-84, 88.
	SHAO Tianyu, LIU Sizhu, XIE Weixin, et al. Application and analysis of DNA bar code technology in species identification of Eulophidae in Heilongjiang Province[J]. Journal of Anhui Agricultural Sciences, 2023, 51(5): 78-84, 88.

采集地点 Collection sites	经度 Longitude(E)(°)	纬度 Latitude(N)(°)	海拔 Altitude(m)	植被类型 Vegetation type
巩留县库尔德宁镇(大莫合) Kurdenen town,Gongliu County(Damohe)	82.788 499	43.198 955	1 320.05	野杏与野苹果等混交林
巩留县库尔德宁镇(72团8连) Kurdenen town,Gongliu County(The company 8 of 72nd)	82.840 172	43.248 247	1 185.53	野杏与野苹果等混交林
新源县阿勒玛勒镇改良场 Alemarez Reform yard,Xinyuan County	83.606 312	43.379 005	1 349.45	野杏与野苹果混交林
霍城县中华福寿山 Fushou Mountain in China Huocheng County	80.783 781	44.434 547	1 077.55	野杏与野樱桃李等混交林
伊宁县吉里于孜镇野杏林 Yining County Jiliyuzi Zhen of wild apricot groves	81.618 832	44.106 504	1 038.42	野杏纯林

采集地点 Collection sites	经度 Longitude(E)(°)	纬度 Latitude(N)(°)	海拔 Altitude(m)	植被类型 Vegetation type
巩留县库尔德宁镇(大莫合) Kurdenen town,Gongliu County(Damohe)	82.788 499	43.198 955	1 320.05	野杏与野苹果等混交林
巩留县库尔德宁镇(72团8连) Kurdenen town,Gongliu County(The company 8 of 72nd)	82.840 172	43.248 247	1 185.53	野杏与野苹果等混交林
新源县阿勒玛勒镇改良场 Alemarez Reform yard,Xinyuan County	83.606 312	43.379 005	1 349.45	野杏与野苹果混交林
霍城县中华福寿山 Fushou Mountain in China Huocheng County	80.783 781	44.434 547	1 077.55	野杏与野樱桃李等混交林
伊宁县吉里于孜镇野杏林 Yining County Jiliyuzi Zhen of wild apricot groves	81.618 832	44.106 504	1 038.42	野杏纯林

序列名称 Sequence name	引物名称 Primer name	引物序列(5’-3’) Primer sequences (5’-3’)	PCR反应条件 PCR reaction conditions
28S rDNA	D2-3551F	CGTGTTGCTTGATAGTGCAGC	94 ℃,5 min;94 ℃,30 s,51 ℃,30 s, 72 ℃,1 min,40个循环;72 ℃,10 min
28S rDNA	D2-4068R	TTGGTCCGTGTTTCAAGACGGG
COI	C1-J-1718	GGAGGATTTGGAAATTGATTAGTTCC	94 ℃,5 min;94 ℃,1 min,45 ℃,1.5 min,72 ℃, 1 min,5个循环;94 ℃,30 s,58~60 ℃,1.5 min, 72 ℃,1 min,35个循环;72 ℃,10 min
	HCO2198	TAAACTTCAGGGTGACCAAAAAATCA
	FWPTF1	CCTGGTTCTTTRATTGGTAATGATC	94℃,5 min;94℃,1 min,45 ℃,1.5 min,72 ℃, 1.25 min,6个循环;94 ℃,1 min,51 ℃,1.5 min, 72 ℃,1.25 min,36个循环;72 ℃,10 min
	Lep-R1	TAAACTTCTGGATGTCCAAAAA

序列名称 Sequence name	引物名称 Primer name	引物序列(5’-3’) Primer sequences (5’-3’)	PCR反应条件 PCR reaction conditions
28S rDNA	D2-3551F	CGTGTTGCTTGATAGTGCAGC	94 ℃,5 min;94 ℃,30 s,51 ℃,30 s, 72 ℃,1 min,40个循环;72 ℃,10 min
28S rDNA	D2-4068R	TTGGTCCGTGTTTCAAGACGGG
COI	C1-J-1718	GGAGGATTTGGAAATTGATTAGTTCC	94 ℃,5 min;94 ℃,1 min,45 ℃,1.5 min,72 ℃, 1 min,5个循环;94 ℃,30 s,58~60 ℃,1.5 min, 72 ℃,1 min,35个循环;72 ℃,10 min
	HCO2198	TAAACTTCAGGGTGACCAAAAAATCA
	FWPTF1	CCTGGTTCTTTRATTGGTAATGATC	94℃,5 min;94℃,1 min,45 ℃,1.5 min,72 ℃, 1.25 min,6个循环;94 ℃,1 min,51 ℃,1.5 min, 72 ℃,1.25 min,36个循环;72 ℃,10 min
	Lep-R1	TAAACTTCTGGATGTCCAAAAA

种 Species	GeneBank登录号 GeneBank accession number		获得寄生蜂数量(头) The number of the parasitoid
种 Species	28S rDNA	COI	野樱桃李 Wild cherry plum	占比 (%)	野杏树 Wild apricot grove	占比 (%)
食蚧点刻跳小蜂 Discodes coccophagus Ratzeburg	OP880422	OP797941	12	5.77	0	0.00
红黄花翅跳小蜂 Microterys rufofulvus Ishii	OP880418	OP797946	2	0.96	0	0.00
园囿花翅跳小蜂 Microterys hortulanus Erdös	OP880423 OP909927	OP797947 OP797942	35	16.83	66	52.80
奇异跳小蜂 Cerapterocerus mirabilis Westwood	OP880430 OP880419	OP797943	38	18.27	31	24.80
赖食蚧蚜小蜂 Coccophagus lycimnia (Walker)	OP880421	OP802581	2	0.96	3	2.40
松毛虫楔缘金小蜂 Pachyneuron solitarium (Hartig)	OP880424	OP797944	98	47.12	15	12.00
提亚长尾啮小蜂 Aprostocetus trjapitzini Kostjukov	OP967184	OP967184	21	10.10	10	8.00