Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (5): 1216-1222.DOI: 10.6048/j.issn.1001-4330.2022.05.021
• Plant Protection·Microbes·Storage and Preservation Processing • Previous Articles Next Articles
ZHAO Yingying1(), ZHANG Jungao2, LI Jin2, LIANG Jing2, GAO Xiangyu1, GU Aixing2(
), LIE Bin2(
)
Received:
2021-12-03
Online:
2022-05-20
Published:
2022-06-09
Correspondence author:
GU Aixing, LIE Bin
Supported by:
赵莹莹1(), 张军高2, 李进2, 梁晶2, 高翔宇1, 顾爱星2(
), 雷斌2(
)
通讯作者:
顾爱星,雷斌
作者简介:
赵莹莹(1997-),女,山东菏泽人,硕士,研究方向为棉花枯萎病生物防治,(E-mail) 1208380386@qq.com
基金资助:
CLC Number:
ZHAO Yingying, ZHANG Jungao, LI Jin, LIANG Jing, GAO Xiangyu, GU Aixing, LIE Bin. Preliminary Study on the Synergistic Effects of Bacillus subtilis KXZ-33 and Chemical Pesticides against Cotton Fusarium wilt[J]. Xinjiang Agricultural Sciences, 2022, 59(5): 1216-1222.
赵莹莹, 张军高, 李进, 梁晶, 高翔宇, 顾爱星, 雷斌. 枯草芽孢杆菌KXZ-33与化学药剂协同防控棉花枯萎病的效果评价[J]. 新疆农业科学, 2022, 59(5): 1216-1222.
处理 Treatment | 抑菌率 Inhibition rate(%) |
---|---|
KXZ-33 | 79.53±10.20a |
KX-4 | 58.66±8.66b |
A57 | 68.50±7.3 |
UB17-1 | 75.51±9.23a |
Twt34 | 71.61±6.1 |
Hwt34 | 56.69±3.02c |
Table 1 The inhibition rate of bacteria from cotton roots against F.oxysporum
处理 Treatment | 抑菌率 Inhibition rate(%) |
---|---|
KXZ-33 | 79.53±10.20a |
KX-4 | 58.66±8.66b |
A57 | 68.50±7.3 |
UB17-1 | 75.51±9.23a |
Twt34 | 71.61±6.1 |
Hwt34 | 56.69±3.02c |
化学杀菌剂 Fungicide | 回归方程 Regression equation | EC50 (mg/L) | R2 Correlation coefficient |
---|---|---|---|
50%甲基硫菌灵可湿性粉剂 Thiophanate-methyl | y=0.609 8x+3.315 4 | 15.840 1 | 0.997 9 |
40%多菌灵悬浮剂 Carbendazim | y=1.221 6x+1.467 9 | 18.018 0 | 0.995 1 |
43%氟菌肟菌酯乳油 Fluomycetin | y=0.177 3x+4.070 8 | 188.827 7 | 0.951 2 |
80%代森锰锌可湿性粉剂 Mancozeb | y=0.258 2x+3.040 2 | 1 987.788 6 | 0.972 8 |
80%乙蒜素乳油 Ethylicin | y=0.668 6x+1.767 8 | 125.748 0 | 0.969 2 |
30%噁霉灵可湿性粉剂 Hymexazo | y=1.047 4x+0.533 0 | 89.221 3 | 0.997 3 |
50%福美双可湿性粉剂 Formetrix | y=0.550 3+3.597 3 | 12.794 0 | 0.978 6 |
Table 2 Laboratory toxicity test of chemical pesticides to F.oxysporum
化学杀菌剂 Fungicide | 回归方程 Regression equation | EC50 (mg/L) | R2 Correlation coefficient |
---|---|---|---|
50%甲基硫菌灵可湿性粉剂 Thiophanate-methyl | y=0.609 8x+3.315 4 | 15.840 1 | 0.997 9 |
40%多菌灵悬浮剂 Carbendazim | y=1.221 6x+1.467 9 | 18.018 0 | 0.995 1 |
43%氟菌肟菌酯乳油 Fluomycetin | y=0.177 3x+4.070 8 | 188.827 7 | 0.951 2 |
80%代森锰锌可湿性粉剂 Mancozeb | y=0.258 2x+3.040 2 | 1 987.788 6 | 0.972 8 |
80%乙蒜素乳油 Ethylicin | y=0.668 6x+1.767 8 | 125.748 0 | 0.969 2 |
30%噁霉灵可湿性粉剂 Hymexazo | y=1.047 4x+0.533 0 | 89.221 3 | 0.997 3 |
50%福美双可湿性粉剂 Formetrix | y=0.550 3+3.597 3 | 12.794 0 | 0.978 6 |
处理 Treatment | 7 d | 14 d | 21 d | 28 d | ||||
---|---|---|---|---|---|---|---|---|
病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect (%) | |
KXZ-33 107cfu/mL | 0.11±0.08c | 72.4±7.3ab | 0.36±0.09bc | 45.2±4.2ab | 0.46±0.04bcd | 37.0±4.3ab | 0.62±0.04b | 20.2±5.5bc |
50%甲基硫菌灵 20 mg/L | 0.31±0.07ab | 21.0±0.1b | 0.35±0.09bc | 47.1±8.0ab | 0.38±0.03cd | 48.9±4.7a | 0.50±0.02c | 35.6±0.3ab |
KXZ-33 107cfu/mL+20 mg/L 甲基硫菌灵 | 0.05±0.04c | 87.7±0.1a | 0.28±0.09b | 57.7±3.7a | 0.32±0.08d | 56.3±5.8a | 0.44±0.06c | 43.7±8.0a |
20 mg/L多菌灵 | 0.20±0.06bc | 50.0±0.1ab | 0.40±0.03bc | 38.4±5.6ab | 0.58±0.03ab | 20.5±3.4b | 0.64±0.04b | 17.8±6.4cd |
KXZ-33 107 cfu/mL+10 mg/L 多菌灵 | 0.04±0.03c | 90.5±0.1a | 0.42±0.09ab | 35.2±2.0ab | 0.56±0.14abc | 24.3±3.1b | 0.57±0.123a | 27.0±4.6bc |
CK | 0.40±0.04a | - | 0.66±0.06a | - | 0.73±0.08a | - | 0.78±0.06a | - |
Table 4 Synergistic control effect of different chemical fungicides and KXZ-33 on Fusarium wilt of cotton
处理 Treatment | 7 d | 14 d | 21 d | 28 d | ||||
---|---|---|---|---|---|---|---|---|
病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect(%) | 病情指数 Condition index | 防病效果 Control effect (%) | |
KXZ-33 107cfu/mL | 0.11±0.08c | 72.4±7.3ab | 0.36±0.09bc | 45.2±4.2ab | 0.46±0.04bcd | 37.0±4.3ab | 0.62±0.04b | 20.2±5.5bc |
50%甲基硫菌灵 20 mg/L | 0.31±0.07ab | 21.0±0.1b | 0.35±0.09bc | 47.1±8.0ab | 0.38±0.03cd | 48.9±4.7a | 0.50±0.02c | 35.6±0.3ab |
KXZ-33 107cfu/mL+20 mg/L 甲基硫菌灵 | 0.05±0.04c | 87.7±0.1a | 0.28±0.09b | 57.7±3.7a | 0.32±0.08d | 56.3±5.8a | 0.44±0.06c | 43.7±8.0a |
20 mg/L多菌灵 | 0.20±0.06bc | 50.0±0.1ab | 0.40±0.03bc | 38.4±5.6ab | 0.58±0.03ab | 20.5±3.4b | 0.64±0.04b | 17.8±6.4cd |
KXZ-33 107 cfu/mL+10 mg/L 多菌灵 | 0.04±0.03c | 90.5±0.1a | 0.42±0.09ab | 35.2±2.0ab | 0.56±0.14abc | 24.3±3.1b | 0.57±0.123a | 27.0±4.6bc |
CK | 0.40±0.04a | - | 0.66±0.06a | - | 0.73±0.08a | - | 0.78±0.06a | - |
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