Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 607-614.DOI: 10.6048/j.issn.1001-4330.2024.03.010

• Horticultural Special Local Products • Previous Articles     Next Articles

Physiological and biochemical characteristics of dominant lactic acid bacteria and cellulolytic bacteria in sunflower By-Products

LI Xiao1(), CHEN Yongcheng1, HUANG Rongzheng1, XU Pingzhu2, ZHANG Fanfan1(), MA Chunhui1()   

  1. 1. College of Animal Science and Technology,Shihezi University,Shihezi Xinjiang 832000,China
    2. Xingjiang Xu zhihua Grassland Ecology Co.,Ltd,Habahe Xinjiang 836700,China
  • Received:2023-08-05 Online:2024-03-20 Published:2024-04-19
  • Correspondence author: ZHANG Fanfan (1989-), male, from Urumqi, Xinjiang, Ph.D, associate professor, master tutor, research direction: forage production and processing,(E-mail)zhangfanfan@shzu.edu.cn;MA Chunhui (1966-), male, from Hami, Xinjiang, Ph.D, professor,doctoral supervisor, research direction: forage production and processing,(E-mail)chunhuima@126.com
  • Supported by:
    China Agriculture Research System(CARS);Xinjiang Uygur Autonomous Region High-quality forage industry technology system project(2022XJSC-Z-02)

向日葵副产物中优势乳酸菌和纤维素分解菌的生理生化特征分析

李肖1(), 陈永成1, 黄嵘峥1, 许平珠2, 张凡凡1(), 马春晖1()   

  1. 1.石河子大学动物科技学院,新疆石河子 832000
    2.新疆蓿之花草原生态有限公司,新疆哈巴河 836700
  • 通讯作者: 张凡凡(1989-),男,新疆乌鲁木齐人,副教授,博士,硕士生导师,研究方向为饲草加工与生产,(E-mail)zhangfanfan@shzu.edu.cn;马春晖(1966-),男,新疆哈密人,教授,博士,硕士生/博士生导师,研究方向为饲草加工与生产,(E-mail)chunhuima@126.com
  • 作者简介:李肖(1998-),男,新疆伊犁人,硕士研究生,研究方向为饲草加工与生产,(E-mail)744364871@qq.com
  • 基金资助:
    农业农村部:国家现代农业产业技术体系项目(CARS);新疆维吾尔自治区优质饲草产业技术体系项目(2022XJSC-Z-02)

Abstract:

【Objective】 This study aims to provide a basis for fermented feeds by sunflower by-products.【Methods】 The dominant lactic acid bacteria and cellulolytic bacteria attached to the surface of sunflower by-products were isolated,extracted and identified,and the physiological and biochemical characteristics of the dominant bacteria were analyzed.【Results】 3 strains of lactic acid bacteria and 4 strains of cellulolytic bacteria were isolated.The 3 strains of lactic acid bacteria obtained were Enterococcus mundtii.Among the 4 strains of cellulolytic bacteria,strains Z2 and Z13 were Bacillus velezensis,X14 was Bacillus aryabhattai,and X4 was Bacillus amyloliquefaciens.3 strains of lactic acid bacteria grew well under the conditions of 4,10,30 and 45℃,3% and 6.5% NaCl,grew at pH 3.5-9,and did not grow at pH3; among the 4 strains of cellulolytic bacteria,the ratio of transparent circle diameter(D)/colony diameter(d) and enzyme activity in order of size were Bacillus amyloliquefaciens > Bacillus velezensis > Bacillus aryabhattai.【Conclusion】 Enterococcus mundtii has strong salt tolerance and a wide range of temperature adaptation,but its acid-producing ability is significantly weaker than that of Lactobacillus plantarum.In the actual weight loss rate of the product,the effect of inoculation with Bacillus amyloliquefaciens is the best.

Key words: sunflower byproduct; lactic acid bacteria; cellulolytic bacteria; isolation; identification; enzymatic activity

摘要:

【目的】 研究向日葵副产物中优势乳酸菌和纤维素分解菌的生理生化特征,为向日葵副产物发酵饲料提供基础。【方法】 对向日葵副产物表面附着优势乳酸菌及纤维素分解菌进行分离、提取鉴定,并分析优势菌种生理生化特征。【结果】 分离出3株乳酸菌和4株纤维素分解菌。得到3株乳酸菌均为蒙氏肠球菌(Enterococcus mundtii)。4株纤维素分解菌中,菌株Z2和Z13为贝莱斯芽孢杆菌(Bacillus velezensis),X14为阿氏芽孢杆菌(Bacillus aryabhattai),X4与解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。3株乳酸菌在4、10、30和45℃及3%和6.5%的NaCl条件下生长良好,在pH 3.5~9可生长,在pH 3环境下不生长;4株纤维素分解菌中透明圈直径(D)/菌落直径(d)比值和酶活性按大小排序均为解淀粉芽孢杆菌>贝莱斯芽孢杆菌>阿氏芽孢杆菌。【结论】 蒙氏肠球菌具有较强耐盐能力且温度适应范围广,但其产酸能力明显弱于植物乳杆菌(Lactobacillus plantarum);4株纤维素分解菌在CMC糖化酶活力、滤纸酶活性及向日葵副产物的实际失重率中,接种解淀粉芽孢杆菌效果最优。

关键词: 向日葵副产物, 乳酸菌, 纤维素分解菌, 分离, 鉴定, 酶活性

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