Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 607-614.DOI: 10.6048/j.issn.1001-4330.2024.03.010
• Horticultural Special Local Products • Previous Articles Next Articles
LI Xiao1(), CHEN Yongcheng1, HUANG Rongzheng1, XU Pingzhu2, ZHANG Fanfan1(
), MA Chunhui1(
)
Received:
2023-08-05
Online:
2024-03-20
Published:
2024-04-19
Correspondence author:
ZHANG Fanfan (1989-), male, from Urumqi, Xinjiang, Ph.D, associate professor, master tutor, research direction: forage production and processing,(E-mail)Supported by:
李肖1(), 陈永成1, 黄嵘峥1, 许平珠2, 张凡凡1(
), 马春晖1(
)
通讯作者:
张凡凡(1989-),男,新疆乌鲁木齐人,副教授,博士,硕士生导师,研究方向为饲草加工与生产,(E-mail)作者简介:
李肖(1998-),男,新疆伊犁人,硕士研究生,研究方向为饲草加工与生产,(E-mail)744364871@qq.com
基金资助:
CLC Number:
LI Xiao, CHEN Yongcheng, HUANG Rongzheng, XU Pingzhu, ZHANG Fanfan, MA Chunhui. Physiological and biochemical characteristics of dominant lactic acid bacteria and cellulolytic bacteria in sunflower By-Products[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 607-614.
李肖, 陈永成, 黄嵘峥, 许平珠, 张凡凡, 马春晖. 向日葵副产物中优势乳酸菌和纤维素分解菌的生理生化特征分析[J]. 新疆农业科学, 2024, 61(3): 607-614.
项目Items | X12 | X13 | Z5 | LP1 | 项目Items | X12 | X13 | Z5 | LP1 |
---|---|---|---|---|---|---|---|---|---|
产酸Acidproduction | + | + | + | + | 葡萄糖Glucose | + | + | + | + |
产气Aerogenesis | - | - | - | - | 乳糖Lactose | + | + | + | + |
发酵类型Fermentation type | 同型Homo | 木糖Xylose | + | + | + | + | |||
4℃ | + | + | + | w | 棉子糖Raffinose | + | + | + | + |
10℃ | + | + | + | ++ | 蔗糖Sucrose | + | + | + | + |
30℃ | + | + | + | ++ | 甘露糖Mannose | + | + | + | + |
45℃ | + | + | + | + | 山梨醇Sorbitol | + | + | + | + |
3%NaCl | + | + | + | + | 鼠李糖Rhamnose | w | w | w | - |
6.5%NaCl | + | + | + | + | 松三糖Melezitose | - | - | - | + |
pH 3 | - | - | - | - | 纤维二糖Cellobiose | + | + | + | + |
pH 3.5 | w | w | w | w | 七叶苷Esculin | + | + | + | + |
pH 4 | + | + | + | + | 蜜二糖Melibiose | w | w | w | + |
pH 5 | + | + | + | ++ | 果糖Fructose | + | + | + | + |
pH 6 | + | + | + | ++ | 海藻糖Trehalose | + | + | + | + |
pH 7 | + | + | + | ++ | 甘露醇Mannitol | + | + | + | + |
pH 8 | + | + | + | ++ | 麦芽糖Maltose | + | + | + | + |
pH 9 | + | + | + | ++ |
Tab.1 Physiological and biochemical characteristics of lactic acid bacteria and carbon source fermentation test
项目Items | X12 | X13 | Z5 | LP1 | 项目Items | X12 | X13 | Z5 | LP1 |
---|---|---|---|---|---|---|---|---|---|
产酸Acidproduction | + | + | + | + | 葡萄糖Glucose | + | + | + | + |
产气Aerogenesis | - | - | - | - | 乳糖Lactose | + | + | + | + |
发酵类型Fermentation type | 同型Homo | 木糖Xylose | + | + | + | + | |||
4℃ | + | + | + | w | 棉子糖Raffinose | + | + | + | + |
10℃ | + | + | + | ++ | 蔗糖Sucrose | + | + | + | + |
30℃ | + | + | + | ++ | 甘露糖Mannose | + | + | + | + |
45℃ | + | + | + | + | 山梨醇Sorbitol | + | + | + | + |
3%NaCl | + | + | + | + | 鼠李糖Rhamnose | w | w | w | - |
6.5%NaCl | + | + | + | + | 松三糖Melezitose | - | - | - | + |
pH 3 | - | - | - | - | 纤维二糖Cellobiose | + | + | + | + |
pH 3.5 | w | w | w | w | 七叶苷Esculin | + | + | + | + |
pH 4 | + | + | + | + | 蜜二糖Melibiose | w | w | w | + |
pH 5 | + | + | + | ++ | 果糖Fructose | + | + | + | + |
pH 6 | + | + | + | ++ | 海藻糖Trehalose | + | + | + | + |
pH 7 | + | + | + | ++ | 甘露醇Mannitol | + | + | + | + |
pH 8 | + | + | + | ++ | 麦芽糖Maltose | + | + | + | + |
pH 9 | + | + | + | ++ |
菌株 Strain and number | 基因库 存取号 Accession number | 近源种 Closest related strain | 与GenBank 中近源种的 相似性 Similarity with the closest described species in Genbank(%) |
---|---|---|---|
X12 | OM841487 | 蒙氏肠球菌 Enterococcus mundtii | 100 |
X13 | OL98461 | 蒙氏肠球菌 Enterococcus mundtii | 99.89 |
Z5 | OM102992 | 蒙氏肠球菌 Enterococcus mundtii | 100 |
X4 | OL774703 | 解淀粉芽孢杆菌 Bacillus amyloliquefaciens | 99.89 |
X14 | OM856838 | 阿氏芽孢杆菌 Bacillus aryabhattai | 99.89 |
Z2 | OL889917 | 贝莱斯芽孢杆菌 Bacillus velezensis | 100 |
Z13 | OL889918 | 贝莱斯芽孢杆菌 Bacillus velezensis | 100 |
Tab.2 Gene sequence analysis of lactic acid bacteria and cellulose-decomposing bacteria
菌株 Strain and number | 基因库 存取号 Accession number | 近源种 Closest related strain | 与GenBank 中近源种的 相似性 Similarity with the closest described species in Genbank(%) |
---|---|---|---|
X12 | OM841487 | 蒙氏肠球菌 Enterococcus mundtii | 100 |
X13 | OL98461 | 蒙氏肠球菌 Enterococcus mundtii | 99.89 |
Z5 | OM102992 | 蒙氏肠球菌 Enterococcus mundtii | 100 |
X4 | OL774703 | 解淀粉芽孢杆菌 Bacillus amyloliquefaciens | 99.89 |
X14 | OM856838 | 阿氏芽孢杆菌 Bacillus aryabhattai | 99.89 |
Z2 | OL889917 | 贝莱斯芽孢杆菌 Bacillus velezensis | 100 |
Z13 | OL889918 | 贝莱斯芽孢杆菌 Bacillus velezensis | 100 |
菌株 Strain and number | 菌种名称 The name of thestrain | 透明圈直径D (cm) | 菌落直径d (cm) | D/d值 |
---|---|---|---|---|
X4 | 解淀粉芽孢杆菌Bacillus amyloliquefaciens | 2.10±0.03a | 0.93±0.01a | 2.25±0.06a |
X14 | 阿氏芽孢杆菌Bacillus aryabhattai | 0.63±0.02b | 0.51±0.01b | 0.81±0.01b |
Z2 | 贝莱斯芽孢杆菌Bacillus velezensis | 0.76±0.01c | 0.44±0.01c | 1.72±0.05c |
Z13 | 贝莱斯芽孢杆菌Bacillus velezensis | 0.79±0.02c | 0.46±0.01c | 1.72±0.01c |
Tab.3 Colony size and transparent circle diameter of different strains
菌株 Strain and number | 菌种名称 The name of thestrain | 透明圈直径D (cm) | 菌落直径d (cm) | D/d值 |
---|---|---|---|---|
X4 | 解淀粉芽孢杆菌Bacillus amyloliquefaciens | 2.10±0.03a | 0.93±0.01a | 2.25±0.06a |
X14 | 阿氏芽孢杆菌Bacillus aryabhattai | 0.63±0.02b | 0.51±0.01b | 0.81±0.01b |
Z2 | 贝莱斯芽孢杆菌Bacillus velezensis | 0.76±0.01c | 0.44±0.01c | 1.72±0.05c |
Z13 | 贝莱斯芽孢杆菌Bacillus velezensis | 0.79±0.02c | 0.46±0.01c | 1.72±0.01c |
[1] | Goes R H T B, Miyagi E S, Oliveira E R, et al. Chemical changes in sunflower silage associated with different additives[J]. Acta Scientiarum Animal Sciences, 2013, 35(1):29-35. |
[2] |
张佳, 王园, 安晓萍, 等. 向日葵副产物的营养特性及在反刍动物中的应用[J]. 中国畜牧兽医, 2021, 48(3):916-924.
DOI |
ZHANG Jia, WANG Yuan, AN Xiaoping, et al. Nutritional characteristics of sunflower by-products and its application in ruminants[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48(3):916-924. | |
[3] | 田亚红, 王巍杰, 王之爽. 向日葵花盘、秸秆发酵生产生物蛋白饲料工艺的研究[J]. 饲料工业, 2013, 34(11):42-45. |
TIAN Yahong, WANG Weijie, WANG Zhishuang. Study on fermentation technology using sunflower head and sunflower stalk to produce bioprotein feed[J]. Feed Industry, 2013, 34(11):42-45. | |
[4] | Mcdonald P, Henderson A R, Heron SJE. The Biochemistry of Silage[M]. Marlow: Chalcombe Publications,1991. |
[5] |
Cai Y, Kumai S, Ogawa M, et al. Characterization and identification of Pediococcus species isolated from forage crops and their application for silage preparation[J]. Applied and Environmental Microbiology, 1999, 65(7):2901-2906.
DOI PMID |
[6] |
张玉琳, 杨寒珺, 李超程, 等. 杂交构树青贮饲料中优良乳酸菌的分离与鉴定[J]. 草地学报, 2022, 30(1):38-45.
DOI |
ZHANG Yulin, YANG Hanjun, LI Chaocheng, et al. Isolation and identification of lactic acid bacterium strain from broussonetia papyrifera silage[J]. Acta Agrestia Sinica, 2022, 30(1):38-45.
DOI |
|
[7] | ZHANG F F, WANG X Z, LU W H, et al. Meta-analysis of the effects of combined homo- and hetero-fermentative lactic acid bacteria on the fermentation and aerobic stability of corn silage[J]. International Journal of Agriculture and Biology, 2018, 20(8):1846-1852. |
[8] |
Zhao C, Wang L H, Ma G M, et al. Cellulase Interacts with lactic acid bacteria to affect fermentation quality,microbial community,and ruminal degradability in mixed silage of soybean residue and corn stover[J]. Animals, 2021, 11(2):334.
DOI URL |
[9] |
Zhang B, Wang L J, Shahbazi A, et al. Dilute-sulfuric acid pretreatment of cattails for cellulose conversion[J]. Bioresource Technology, 2011, 102(19):9308-9312.
DOI PMID |
[10] | 宫秀杰, 钱春荣, 于洋, 等. 近年纤维素降解菌株筛选研究进展[J]. 纤维素科学与技术, 2021, 29(2):68-77. |
GONG Xiujie, QIAN Chunrong, YU Yang, et al. Progress on screening of cellulose degrading strains in recent years[J]. Journal of Cellulose Science and Technology, 2021, 29(2):68-77. | |
[11] | 许从峰, 艾士奇, 申贵男, 等. 木质纤维素的微生物降解[J]. 生物工程学报, 2019, 35(11):2081-2091. |
XU Congfeng, AI Shiqi, SHEN Guinan, et al. Microbial degradation of lignocelluloses[J]. Chinese Journal of Biotechnology, 2019, 35(11):2081-2091.
DOI PMID |
|
[12] |
Hasunuma T, Okazaki F, Okai N, et al. A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology-Science Direct[J]. Bioresource Technology, 2013, 135:513-522.
DOI PMID |
[13] | 戴芸芸, 钟卫鸿. 细菌降解木质纤维素的研究进展[J]. 化学与生物工程, 2016, 33(6):11-16. |
DAI Yunyun, ZHONG Weihong. Research Progress on Degradation of Lignocellulose by Bacteria[J]. Chemistry & Bioengineering, 2016, 33(6):11-16. | |
[14] |
杨杨, 石超, 郭旭生. 高寒草甸魏斯氏乳酸菌的分离鉴定及理化特性研究[J]. 草业学报, 2014, 23(1):266-275.
DOI |
YANG Yang, SHI Chao, GUO Xusheng. Characterization and identification of Weissella species isolated from Kobresia littledalei growing in alpine meadows[J]. Acta Prataculturae Sinica, 2014, 23(1):266-275.
DOI |
|
[15] |
陈堞, 林凯程, 林沁, 等. 大米草中耐盐乳酸菌的分离鉴定及对大米草青贮品质的影响[J]. 草地学报, 2020, 28(2):565-570.
DOI |
CHEN Die, LIN Kaicheng, LIN Qin, et al. Isolation and identification of salt-tolerant lactic acid bacteria in Spartina anglica hubb.and its effect on the quality of silage[J]. Acta Agrestia Sinica, 2020, 28(2):565-570.
DOI |
|
[16] |
尹雪, 郭雪峰, 刘俊峰, 等. 盐穗木青贮中乳酸菌的分离及筛选[J]. 中国农业科学, 2018, 51(14):2825-2834.
DOI |
YIN Xue, GUO Xuefeng, LIU Junfeng, et al. Isolation and identification of lactic acid bacteria from Halostachys Caspica silage[J]. Scientia Agricultura Sinica, 2018, 51(14):2825-2834.
DOI |
|
[17] |
王得武, 姚拓, 杨巧丽, 等. 高效稳定纤维素分解菌群筛选及其分解特性研究[J]. 草业学报, 2014, 23(2):253-259.
DOI |
WANG Dewu, YAO Tuo, YANG Qiaoli, et al. Screening and degradation characterization of efficient and stable cellulose degrading microbial communities[J]. Acta Prataculturae Sinica, 2014, 23(2):253-259.
DOI |
|
[18] | 李红亚, 李术娜, 王树香, 等. 解淀粉芽孢杆菌MN-8对玉米秸秆木质纤维素的降解[J]. 应用生态学报, 2015, 26(5):1404-1410. |
LI Hongya, LI Shuna, WANG Shuxiang, et al. Degradation of lignocellulose in the corn straw by Bacillus amyloliquefaciens MN-8[J]. Chinese Journal of Applied Ecology, 2015, 26(5):1404-1410.
PMID |
|
[19] | 凌代文. 乳酸细菌分类鉴定及实验方法[J]. 微生物学通报, 1999, 26(1):23. |
LING Daiwen. Classification,identification and experimental methods of lactic acid bacteria[J]. Microbiology, 1999, 26(1):23. | |
[20] | 高建民, 翁海波, 席宇, 等. 一株嗜热嗜酸纤维素酶高产霉菌分离鉴定及其酶学性质研究[J]. 微生物学通报, 2007, 34(4):715-718. |
GAO Jianmin, WENG Haibo, XI Yu, et al. Isolation and identification on a thermoacidophilic fungus of high-producing cellulase and the characteristics of its enzyme[J]. Microbiology, 2007, 34(4):715-718. | |
[21] | 房兴堂, 陈宏, 赵雪锋, 等. 秸秆纤维素分解菌的酶活力测定[J]. 生物技术通讯, 2007, 18(4):628-630. |
FANG Xingtang, CHEN Hong, ZHAO Xuefeng, et al. Determination of Enzyme Activity of Straw Cellulose-Decomposing Microorganisms[J]. Letters in Biotechnology, 2007, 18(4):628-630. | |
[22] |
孙美娜, 张凡凡, 王永强, 等. 棉花秸秆纤维素降解菌的筛选鉴定与降解棉秆效果研究[J]. 新疆农业科学, 2018, 55(1):16-23.
DOI |
SUN Meina, ZHANG Fanfan, WANG Yongqiang, et al. Screening and Identification of Cellulolytic Strains from Cotton Straw and Its Effect on Degradation of Cotton Stalk[J]. Xinjiang Agricultural Sciences, 2018, 55(1):16-23.
DOI |
|
[23] |
Kumar S, Tamura K, Nei M. MEGA3:Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment[J]. Briefings in Bioinformatics, 2004, 5(2):150-163.
DOI URL |
[24] | 郭刚, 霍文婕, 刘强, 等. 玉米秸秆青贮饲料发酵早期优良乳酸菌的分离和鉴定[J]. 畜牧与兽医, 2016, 48(7):60-64. |
GUO Gang, HUO Wenjie, LIU Qiang, et al. Isolation and identification of good lactic acid bacteria in early fermentation of corn straw silage[J]. Animal Husbandry & Veterinary Medicine, 2016, 48(7):60-64. | |
[25] |
司丙文, 王宗礼, 孙启忠, 等. 山竹岩黄芪青贮中优质乳酸菌的分离和鉴定[J]. 草地学报, 2012, 20(1):166-170.
DOI |
SI Bingwen, WANG Zongli, SUN Qizhong, et al. Isolation and identification of high-quality lactic acid bacteria in Hedysarum fruticosum Pall. Silage[J]. Acta Agrectir Sinica, 2012, 20(1):166-170. | |
[26] |
Lindow S E, Brandl M T. Microbiology of the phyllosphere[J]. Appl Environ Microbiol, 2003, 69(4):1875-1883.
DOI URL |
[27] |
Yu A O, Leveau J H J, Marco M L. Abundance, diversity and plant-specific adaptations of plant-associated lactic acid bacteria[J]. Environ Microbiol Rep, 2020, 12(1):16-29.
DOI URL |
[28] | 王智伟. 白蚁体内产纤维素酶细菌的分离鉴定及其相关基因的载体构建与原核表达[D]. 杨凌: 西北农林科技大学, 2018. |
WANG Zhiwei. Isolation and identification of cellulase-producing bacteria in termites and their related gene vector construction and prokaryotic expression[D]. Yangling: Northwest A & F University, 2018. | |
[29] | 熊乙, 欧翔, 贾蓉, 等. 阿氏芽孢杆菌应用研究进展[J]. 生物技术, 2018, 28(3):302-306. |
XIONG Yi, OU Xiang, JIA Rong, et al. Research progress in application of Bacillus aryabhattai[J]. Biotechnology, 2018, 28(3):302-306. | |
[30] | 熊乙. 木质纤维素降解菌的筛选鉴定及降解产物研究[D]. 太谷: 山西农业大学, 2019. |
XIONG Yi. Screening and identification of lignocellulose-degrading bacteria and study on degradation products[D]. Taigu: Shanxi Agricultural University, 2019. | |
[31] | 任津莹, 陈鹏. 一株贝莱斯芽孢杆菌的分离鉴定及其生物学特性研究[J]. 饲料研究, 2022, 45(2):79-82. |
REN Jinying, CHEN Peng. Isolation,identification and biological characteristics of a strain of Bacillus velezensis[J]. Feed Research, 2022, 45(2):79-82. | |
[32] |
张凡凡, 张玉琳, 王旭哲, 等. 纤维素分解菌与布氏乳杆菌联合接种对青贮玉米发酵品质、有氧稳定性和瘤胃降解参数的影响[J]. 动物营养学报, 2021, 33(3):1735-1746.
DOI |
ZHANG Fanfan, ZHANG Yulin, WANG Xuzhe, et al. Effects of cellulose decomposing bacteria and Lactobacillus buchneri combined culture on fermentation quality,aerobic stability and rumen degradation parameters of corn silage[J]. Chinese Journal of Animal Nutrition, 2021, 33(3):1735-1746. |
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