EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot

doi:10.6048/j.issn.1001-4330.2023.09.018

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (9): 2231-2238.DOI: 10.6048/j.issn.1001-4330.2023.09.018

• Plant Protection·Microbes • Previous Articles Next Articles

EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot

QIN Pengliang¹(), ZHOU Xiao², Kahsay Tadesse Mawcha¹, WANG Shuang¹, LI Jiaqi¹, LIU Ying¹, ZHANG Na¹, YANG Wenxiang¹()

1. Technological Innovation Centre for Biological Control of Crop Diseases and Insect Pests of Hebei Province / College of Plant Protection,Hebei Agricultural University, Baoding Hebei 071001, China
2. Hebei Plant Protection and Quarantine General Station,Shijiazhuang 050000, China

Received:2022-12-28 Online:2023-09-20 Published:2023-09-19
Correspondence author: YANG Wenxiang (1966-), female,native place; Baoding, Hebei, Ph.D., professor, doctoral surpervisor research field; the prevention and control of major wheat diseases, (E-mail)wenxiangyang2003@163.com
Supported by:
National key research and development project(2017YFD020170704);Hebei Province industrial system wheat innovation team(HCT2018010204);Key research and development project of Hebei(19226507D)

小麦品种衡观35抗茎基腐病EMS突变及其鉴定

秦鹏亮¹(), 周霄², 王爽¹, 李佳琪¹, 刘颖¹, 张娜¹, 杨文香¹()

1.河北农业大学植物保护学院植物病理学系/河北省农作物病虫害生物防治技术创新中心/国家北方山区农业工程技术研究中心,河北保定 071001
2.河北省植保植检总站,石家庄 050000

通讯作者: 杨文香(1966-),女,河北保定人,教授,博士,硕士生/博士生导师,研究方向为小麦病害防控,(E-mail)wenxiangyang2003@163.com
作者简介:秦鹏亮(1994-),男,陕西宝鸡人,硕士研究生,研究方向为小麦叶锈菌致病机制,(E-mail)1120469245@qq.com
基金资助:
国家重点研发计划项目(2017YFD020170704);河北省产业体系小麦创新团队(HCT2018010204);河北省重点研发计划项目(19226507D)

Abstract

Abstract:

【Objective】 Study EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot.【Methods】 In this study, different concentrations of ethyl methanesul fonate (EMS) were employed on wheat seeds of cultivar Hengguan 35.The untreated Hengguan 35 was used as the susceptible control, and Shiyou 17 was used as the moderately resistant control.Through artificial inoculation of Fusarium pseudograminearum, the resistance of the mutagenized material was identified, and the M₃ generation material resistant to FCR was obtained by adding generation in the greenhouse.【Results】 The EMS concentration suitable for Hengguan 35 mutation was finally determined to be 0.4%, and seven mutants showing immunity at the plant stage were obtained from 106 M₃ Hengguan 35 materials.The overall disease index of the mutant materials at seedling and adult stages was 34.93% and 34.62% lower than those of the susceptible control, respectively.【Conclusion】 After EMS mutation of wheat Hengguan 35, an appropriate mutation concentration of 0.4% was determined. A total of 7 immune resistant wheat FCR were screened from the M3 generation of the tested mutant varieties.

Key words: Fusarium crown rot (FCR); EMS; mutagenesis; mutant; resistance identification

摘要：

【目的】研究小麦品种衡观35抗茎基腐病EMS突变及其鉴定。【方法】利用不同浓度的甲基磺酸乙酯(EMS),对小麦品种衡观35进行诱变,以未经诱变的衡观35为感病对照,以石优17为中度抗病对照。人工接种假禾谷镰刀菌,对诱变材料进行抗性鉴定,经温室加代获得抗茎基腐病M₃代材料。【结果】适于衡观35突变的EMS浓度为0.4%,并且从106株M₃衡观35材料中获得7株期表现免疫的突变株,突变体材料苗期和成株期整体病情指数比感病对照分别降低34.93%和34.62%。【结论】EMS突变小麦衡观35,适宜的突变浓度为0.4%,共从供试突变品种M₃代中筛选获得了7份免疫的抗小麦茎基腐病的资源。

关键词: 小麦茎基腐病, EMS, 诱变, 突变株, 抗性鉴定

CLC Number:

S512
S188

QIN Pengliang, ZHOU Xiao, Kahsay Tadesse Mawcha, WANG Shuang, LI Jiaqi, LIU Ying, ZHANG Na, YANG Wenxiang. EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2231-2238.

秦鹏亮, 周霄, 王爽, 李佳琪, 刘颖, 张娜, 杨文香. 小麦品种衡观35抗茎基腐病EMS突变及其鉴定[J]. 新疆农业科学, 2023, 60(9): 2231-2238.

Figures/Tables 7

References 33

[1]	Fernandze M R, Jefferson P G. Fungal populations in roots and crowns of common and durum wheat in Saskatchewan[J]. Canadian Journal of Plant Pathology, 2004,(3):325-334.
[2]	周海峰, 杨云, 牛亚娟, 等. 小麦茎基腐病的发生动态与防治技术[J]. 河南农业科学, 2014, 43(5):114-117.
	ZHOU Haifeng, YANG Yun, NIU Yajuan, et al. Occurrence and Control Methods of Crown Rot of Wheat[J]. Journal of Henan Agricultural Science, 2014, 43(5):114-117.
[3]	YANG Xia, PAN Yubo, SINGH Pawan K, et al. Investigation and Genome-wide Association Study for Fusarium Crown Rot Resistance in Chinese Common Wheat[J]. BMC Plant Biology, 2019, 19(1): 153-167. DOI PMID
[4]	冯小军, 郭海鹏, 贺亚红, 等. 小麦茎基腐病田间化学防治研究[J]. 西北农业学报, 2019, 28(9):1515-1521.
	FENG Xiaojun, GUO Haipeng, HE Yahong, et al. Study on Field Chemical Control of Wheat Crown Rot[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2019, 28(9):1515-1521.
[5]	蒲乐凡, 任慧, 欧杨晨, 等. 小麦茎基腐病和赤霉病抗源筛选及关联SNP位点分析[J]. 麦类作物学报, 2020, 40(7):780-788.
	PU Lefan, REN Hui, OU Yangchen, et al. Screening of Germplasms Resistance to Crown Rot and Fusarium Head Blight and the Associated SNPs in Wheat[J]. Journal of Triticeae Crops, 2020, 40(7):780-788.
[6]	郑中玲. 河南省小麦茎基腐病发生因素分析及其防治研究[D]. 新乡: 河南科技学院, 2020.
	ZHENG Zhongling. Study on the Occurrence Factors and Control of Wheat Stem-based Rot in Henan Province[D]. Xinxiang: Henan Institute of Science and Technology, 2020.
[7]	赵越. EMS诱导小麦突变及优良突变鉴选[D]. 杨凌: 西北农林科技大学, 2020.
	ZHAO Yue. EMS-induced Wheat Mutation and Selection of Superior Mutation[D]. Yangling: Northwest A&F University, 2020.
[8]	LI Wenjie, GUO Huijun, WANG Yongbin, et al. Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING[J]. Genes & Genomics, 2016, 39(4):387-390.
[9]	Greene Elizabeth A, Codomo Christine A, Taylor Nicholas E, et al. Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis[J]. Genetics, 2003, 164(2):731-740. DOI PMID
[10]	Henry Isabelle M, Nagalakhmi Ugrappa, Lieberman Meric C, et al. Efficient genome-wide detection and cataloging of EMS-induced mutations using exome capture and next-generation sequencing[J]. Plant Cell, 2014, 26(4):1382. DOI URL
[11]	Mitter V, Zhang M C, Liu C J, et al. A high-throughput glasshouse bioassay to detect crown rot resistance in wheat germplasm[J]. Plant Pathology, 2006, 55(3): 433-441. DOI URL
[12]	袁凯, 张伟, 温宏伟, 等. EMS诱导济麦22突变体库的构建及表型变异分析[J]. 植物遗传资源学报, 2021, 22(4):979-988.
	YUAN Kai, ZHANG Wei, WEN Hongwei, et al. EMS-induced Mutagenesis and Phenotypic Variation Analysis in Wheat Variety Jimai 22[J]. Journal of Plant Genetic Resources, 2021, 22(4):979-988.
[13]	薛芳, 褚洪雷, 胡志伟, 等. EMS 对新春 11 小麦抗性淀粉和农艺性状的诱变效果[J]. 麦类作物学报, 2010, 30(3):431-434.
	XUE Fang, CHU Honglei, HU Zhiwei, et al. Mutation effect on resistant starch content and agronomic traits of Xinchun 11 treated by EMS[J]. Journal of Triticeae Crops, 2010, 30(3):431-434.
[14]	Mago R, Spielmeyer W, Lawrence G J, et al. Resistance genes for rye stem rust(SrR) and barley powdery mildew(Mla) are located in synthetic regions on short arm of chromosome[J]. Genome, 2004, 47:112-121. PMID
[15]	张维宏, 安哲, 范学锋, 等. EMS 诱导小麦 TcLr19 感叶锈病突变体的筛选[J]. 华北农学报, 2016, 31(4):88-93. DOI
	ZHANG Weihong, AN Zhe, FAN Xuefeng, et al. Screening of mutants susceptible to puccina triticina in TcLr19 Induced by EMS[J]. Acta Agriculturae Boreali-Sinica, 2016, 31(4):88-93.
[16]	孙玉龙, 朱欣欣, 何瑞士, 等. EMS 诱导的盛农1号小麦突变体筛选与鉴定[J]. 麦类作物学报, 2018, 38(7):782-790.
	SUN Yulong, ZHU Xinxin, HE Ruishi, et al. Selection and identification of the EMSInduced wheat mutants from Shengnong 1[J]. Journal of Triticeae Crops, 2018, 38(7):782-790.
[17]	Yasuit, Sasaki, Matsukii, et al. Waxy endosperm mutants of bread wheat (Triticum aestivum L.) and their starch properties[J]. Breeding Science, 1997, 47(2):161-163.
[18]	霍燕, 张鹏, 任丽娟, 等. 小麦茎基腐病苗期快速接种鉴定方法研究[J]. 江西农业学报, 2010, 22(8):93-96.
	HUO Yan, ZHANG Peng, REN Lijuan, et al. Comparison of Inoculation Methods for Severity Assessment of Crown Rot Disease in Wheat Seedlings[J]. Acta Agriculturae Jiangxi, 2010, 22(8):93-96.
[19]	张纪元, 张平平, 姚金保, 等. 以EMS诱变创制软质小麦宁麦9号高分子量谷蛋白亚基突变体[J]. 作物学报, 2014, 40(9):1579-1584.
	ZHANG Jiyuan, ZHANG Pingping, YAO Jinbao, et al. EMS Induced HMW-GS Mutants from Soft Wheat Ningmai 9[J]. Acta Agronomica Sinica, 2014, 40(9):1579-1584. DOI URL
[20]	邓渊钰, 孙海燕, 李伟, 等. 小麦茎基腐镰孢菌致病力的快速测定[J]. 麦类作物学报, 2016, 36(11):1547-1552.
	DENG Yuanyu, SUN Haiyan, LI Wei, et al. Rapid Virulence Assessment of Fusarium pathogens Causing Crown Rot of wheat[J]. Journal of Triticeae Crops, 2016, 36(11):1547-1552.
[21]	王佐乾, 潘竹青, 张舒, 等. 湖北省黄连炭疽病的病原鉴定及其生物学特性[J]. 湖北农业科学, 2020, 59(24):117-121.
	WANG Zuoqian, PAN Zhuqing, ZHANG Shu, et al. Identification and biological characteristics of the pathogen causing anthracnose on goldthread in Hubei Province[J]. Hubei Agricultural Sciences, 2020, 59(24):117-121.
[22]	Wildermuth G B, Mcnamara R B, Quick J S. Crown depth and susceptibility to crown rot in wheat[J]. Euphytica, 2001, 122(2):397-405. DOI URL
[23]	Raju T N, Tumer M A, Davies P A, et al. Development of crown rot resistant gernplasm.In: Brettell RIS, Nicol J M, eds.1st International Crown Rot Workshop for Wheat Improvement[C]. Narrabri, NAS, Australia: Crown Rot of Wheat Workshop, 2012:60.
[24]	Li X, Liu C, Chakraborty S, et al. A simple method for the assessment of crown rot disease severity in wheat seedlings inoculated with Fusarium pseudograminearum[J]. Journal of Phytopathology, 2008, 156:751-754. DOI URL
[25]	Mago Rohit, Till Bradley, Periyannan Sambasivam, et al. Generation of loss-of-function mutants for wheat rust disease resistance gene cloning[J]. Methods in Molecular Biology, 2017, 1659:199-205. DOI PMID
[26]	LI Tao; Luo Meng; Qian Dan, et al. Evaluation of Phenotypic Variation of EMS-induced Mutants of Chinese Wheat Landraces Huangfangzhu and Haiyanzhong Resistant to Fusarium Head Blight[J]. Journal of Plant Genetic Resources, 2016, 17(6):1092-1098.
[27]	赵天祥, 孔秀英, 周荣华, 等. EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析[J]. 中国农业科学, 2009, 42(3):755-764.
	ZHAO Tianxiang, KONG Xiuying, ZHOU Ronghua, et al. Morphological Identification and Analysis of EMS Mutants from Hexaploid Wheat Cultivar Yanzhan 4110[J]. Scientia Agricultura Sinica, 2009, 42(3):755-764.
[28]	温日宇, 刘建霞, 肖东东, 等. EMS诱变对藜麦种子萌发及幼苗生理特性的影响[J/OL]. 分子植物育种:1-10[2023-08-02].
	WEN Riyu, LIU Jianxia, XIAO Dongdong, et al. Effects of EMS Mutagenesis on Seed Germination and Physiological Characteristics of Quinoa Seedlings[J/OL]. Molecular Plant Breeding, 1-10[2023-08-02].
[29]	卢银, 刘梦洋, 王彦华, 等. EMS处理对大白菜种子萌发及主要生化指标的影响[J]. 中国蔬菜, 2014, 2(11): 20-24.
	LU Yin, LIU Mengyang, WANG Yanhua, et al. Effects of EMS Treatment on Germination and Biochemical Characteristics of Chinese Cabbage Seed[J]. China Vegetables, 2014, 2(11): 20-24.
[30]	曲高平, 孙妍妍, 庞红喜, 等. 甘蓝型油菜 EMS 突变体库构建及抗除草剂突变体筛选[J]. 中国油料作物学报, 2014, 36(1): 25-31. DOI
	QU Gaoping, SUN Yanyan, PANG Hongxi, et al. EMS mutagenesis and ALS-inhibitor herbicide-resistant mutants of Brassica napus L.[J]. Chinese Journal of Oil Crop Sciences, 2014, 36(1): 25-31. DOI
[31]	金京京. 普通小麦品种抗茎基腐病全基因组关联分析及蛋白质组学研究[D]. 保定: 河北农业大学, 2020.
	JIN Jingjing. Genome Wide Association Analysis and Proteomics of Resistance to Fusarium Causing Crown Rot in common wheat varieties[D]. Baoding: Hebei Agricultural University, 2020.
[32]	YANG Xia, ZHONG Shaobin, ZHANG Qijun, et al. Loss-of-function of the Dirigent Gene TaDIR-B1 Improves Resistance to Fusarium Crown Rot in Wheat[J]. Plant Biotechnology Journal, 2021, 19(5):866-868. DOI URL
[33]	许云峰, 蒋方山, 郭营, 等. EMS诱导小麦品种烟农15突变体的鉴定和EST-SSR分析[J]. 核农学报, 2008,(4):410-414.
	XU Yunfeng, JIANG Fangshan, GUO Ying, et al. Identification and EST-SSR Analysis of Mutants From Wheat Variety “Yannong 15” Induced by EMS[J]. Journal of Nuclear Agricultural Sciences, 2008,(4):410-414.

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	26	4.76	127	23.26	111	20.33	102	18.68	94	17.22	86	15.75	546	53.52^a
M₀衡观35 M₀ Hengguan 35	106	14.50	148	20.25	182	24.90	137	18.74	77	10.53	81	11.08	731	44.76^b
石优17Shiyou 17	11	4.01	43	15.69	67	24.45	64	23.36	57	20.80	32	11.68	274	55.26^a

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	26	4.76	127	23.26	111	20.33	102	18.68	94	17.22	86	15.75	546	53.52^a
M₀衡观35 M₀ Hengguan 35	106	14.50	148	20.25	182	24.90	137	18.74	77	10.53	81	11.08	731	44.76^b
石优17Shiyou 17	11	4.01	43	15.69	67	24.45	64	23.36	57	20.80	32	11.68	274	55.26^a

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	4	8.00	12	24.00	14	28.00	9	18.00	8	16.00	3	6.00	50	45.60^a
M₃衡观35 M₃ Hengguan 35	25	20.83	28	23.33	51	42.50	16	13.33	0	0.00	0	0.00	120	29.67^b
石优17Shiyou 17	3	6.98	25	58.14	7	16.28	6	13.95	2	4.65	0	0.00	43	30.23^b

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	4	8.00	12	24.00	14	28.00	9	18.00	8	16.00	3	6.00	50	45.60^a
M₃衡观35 M₃ Hengguan 35	25	20.83	28	23.33	51	42.50	16	13.33	0	0.00	0	0.00	120	29.67^b
石优17Shiyou 17	3	6.98	25	58.14	7	16.28	6	13.95	2	4.65	0	0.00	43	30.23^b

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	0	0.00	12	24.00	14	28.00	10	20.00	10	20.00	4	8.00	50	52.00^a
M₃衡观35 M₃ Hengguan 35	7	5.83	40	30.00	55	45.83	18	15.00	0	0.00	0	0.00	120	34.00^b
石优17Shiyou 17	1	2.33	26	60.47	7	16.28	7	16.28	2	4.65	0	0.00	43	32.09^b

EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot

小麦品种衡观35抗茎基腐病EMS突变及其鉴定

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品种 Variety	千粒重(g) 1000 grain weight (g)
品种 Variety	0级 Grade 0	Ⅰ级 Grade Ⅰ	Ⅱ级 Grade Ⅱ	Ⅲ级 Grade Ⅲ
衡观35 Hengguan 35	40.23^b	32.51^b	28.10^b	15.18^b
M₃衡观35 M₃ Hengguan 35	43.96^a	38.51^a	32.72^a	20.10^a
石优17 Shiyou 17	40.37^b	34.43^b	29.13^b	17.16^b

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[7]	ZHANG Hong, WANG Hao-jie, MAO Jian-cai, LI Jun-hua, ZHAI Wen-qiang, YI Hong-ping, Hanan Eizenberg, Yaakov Tadmor. Identification of Resistance of Nine Varieties of Cucumis melo L. to Orobanche aegyptiaca Using a Polythene Bags-based Hydroponic Culture Method [J]. Xinjiang Agricultural Sciences, 2019, 56(9): 1619-1625.
[8]	ZHANG Xue-jun, GUO LI-xia, ZHANG Jian, YANG Yong, LI Mei-hua, YI Hong-ping. Establishment of Identification Methods and Germplasm Resource Evaluation of Cucumis melo ssp.melo Downy Mildew at Seedling Stage [J]. Xinjiang Agricultural Sciences, 2019, 56(5): 899-909.
[9]	MAO Jian-cai , WANG Hao-jie , LI Jun-hua , ZHAI Wen-qiang. Prevention and Control of Replanted Muskmelon Virus Disease by Fly Net and Pathogens Identified by RT-PCR [J]. Xinjiang Agricultural Sciences, 2019, 56(12): 2304-2311.
[10]	ZHOU Yong, ZHANG Xiao-hong, Miriguli Mamuti, MA Dun, ZHOU Xiao-yun. Research on Exogenous Hormones Sensitivity on Plant Height of a Semi-Dwarf Mutant in Upland Cotton [J]. Xinjiang Agricultural Sciences, 2018, 55(3): 405-413.
[11]	HAN Hong-wei, LIU Hui-fang, HAN Sheng, ZHUANG Hong-mei, SHE Jian-hua, WANG qiang, WANG Hao. Identification of Resistance to Acidovoraxavenae subsp. Citrulli among 17 Cantaloupe Cultivars at the Seedling Stage [J]. Xinjiang Agricultural Sciences, 2018, 55(10): 1863-1869.
[12]	PENG Jin-feng,YAO Zhao-qun,BAO Ya-zhou,CAO Xiao-lei,CHEN Mei-xiu, CHEN Hong-jiao,ZHAO-Si-feng. Resistance Identification of Different Muskmelon Varieties in Xinjiang to Orobanche aegyptiaca [J]. Xinjiang Agricultural Sciences, 2018, 55(1): 95-104.
[13]	CHEN Guo;CHEN Xun-ji;LI Jian-ping;HAO Xiao-yan;CHANG Xiao-chun;Zumuremu;ZHENG Jun;HUANG Quan-sheng. Identification of the Mutator Insertional Mutants in ZmCIPK23 [J]. , 2017, 54(7): 1185-1190.
[14]	SHI Bi-xian;ZHU Ming-cheng;WAGN Qi-cai;GU Yuan-guo;YU Bo-cheng;CHEN Yue-hua;LI Qiang. Present Situation, Existing Problems and Development Strageties of Peanut Production in Xinjiang [J]. , 2017, 54(3): 574-584.
[15]	ZHANG Jin-bo;LI Bo;YANG Yang;HU Wen-ran;CHEN Fan-yuan;XIE Li-xia;FAN Ling. Genomi-wide Investigation and Expression Analysis of CAD Gene Family in Gossypium hirsutum [J]. , 2016, 53(7): 1177-1187.