Isolation and identification of the pathogen for potato black scurf in Xinjiang

doi:10.6048/j.issn.1001-4330.2024.07.024

Abstract

Abstract:

【Objective】To isolate and identify the pathogen causing potato black scurf in Xinjiang in the hope of providing a foundation for screening of control agents and establishment of control technology. 【Methods】Potato tubers were collected from Jimsar, Qitai, Dabancheng and Zhaosu in Xinjiang, and Rhizoctonia solaniwere isolated from tubers with potato black scurf by tissue separation method. Anastomosis groups of Rhizoctonia solaniwere identified by paired culture and ITS sequence analysis. 【Results】The four strains isolated from Jimsar, Qitai, Dabancheng and Zhaosu were all able to cause the symptoms of potato black scurf on potato tubers. The isolated strains were Anastomosis groups AG3. 【Conclusion】Anastomosis Groups AG3 is the main pathogenic group of potato black scurf in Xinjiang.

Key words: potato black scurf; isolation and identification; anastomosis groups

摘要：

【目的】分离鉴定引起新疆马铃薯黑痣病病原菌,为筛选防治该病药剂奠定基础。【方法】从新疆吉木萨尔县、奇台县、乌鲁木齐市达坂城区和昭苏县等马铃薯种植区采集马铃薯块茎,通过组织分离法从带有马铃薯黑痣病的块茎分离病原菌,利用电子显微镜观察病原菌的形态特征,进行对峙培养和ITS序列分析,鉴定融合群。【结果】从新疆吉木萨尔县、奇台县、乌鲁木齐市达坂城区和昭苏县分离的4株病原菌均能使马铃薯块茎出现马铃薯黑痣病症状,分离菌株均为AG3融合群。【结论】AG3融合群是新疆马铃薯黑痣病的主要致病群。

关键词: 马铃薯黑痣病, 分离鉴定, 融合群

CLC Number:

S436.32

SHEN Hongfei, JIA Fenglian, LIU Yi, WU Yan, YANG Ruwei, SUN Hui, LI Guangyue. Isolation and identification of the pathogen for potato black scurf in Xinjiang[J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1766-1771.

洪飞, 贾丰莲, 刘易, 吴燕, 杨茹薇, 孙慧, 李广悦. 新疆马铃薯黑痣病病原菌的分离及定[J]. 新疆农业科学, 2024, 61(7): 1766-1771.

Figures/Tables 5

Tab.1 Primers for pathogen identification

引物名称 Primer name	引物序列(5'-3') Primer sequence(5'-3')
ITS1	TCCGTAGGTGAACCTGCGG
ITS4	TCCTCCGCTTATTGATATGC
AG-2-1-F	CAAAGGCAATGGGTTATTGGAC
AG-2-1-R	CCTGATTTGAGATCAGATCATAAAG
Rs1F2	TTGGTTGTAGCTGGTCTATTT
AG-3PR2	ACACTGAGATCCAGCTAATA

Fig.1 Changes of morphological characteristics of pathogen isolated from potato sample infected with R.solani Note: A-C is the hyphal growth characteristic; E is the colony of pathogen incubatedon in PDA media for 72 hours, F is the colony of pathogen incubatedon in PDA media for 96 hours, and G is the colony of pathogen incubatedon in PDA media for 168 hours

Fig.2 Verification of pathogenicity of the isolated strains Note: CK is harvested tuber from soil without tuber; XJ1-4 was a tieback strain isolated from tuber and harvested tuber

Fig.3 Gel electrophoresis of the PCR amplification with specific primer Note: A is the electrophoretic PCR amplification of universal primer ITS1/IT4 in ITS region of the original pathogen, M is Star Marker D2000 PlusⅡ, 1-4 is strain XJ1-4 isolated from Xinjiang potato.The B diagram is an electrophoretic diagram of PCR amplification verification of pathogens reisolated after tieback.1-4 are universal primers ITS1/IT4.5-8 is the specific primer AG-2-1-F/R, and 9-12 is the specific primer Rs1F2/AG-3PR2

Fig.4 Hyphal fusion reaction of the Rhizoctonia solani isolated from potato with standard strain AG3-PT

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