Evaluation of 294 varieties of brassica rapa cruciferae germplasm for drought resistance throughout the growing season

doi:10.6048/j.issn.1001-4330.2025.07.009

Abstract

Abstract:

【Objective】 Drought is one of the main factors affecting the production of cultivated mustard rapeseed in Xinjiang. This project aims to identify the drought resistance of mustard rapeseed during the whole growth period and screen the materials with strong drought resistance, so as to provide germplasm resources and theoretical basis for drought resistance breeding. 【Methods】 In this study, 294 mustard rapeseed germlines were set up in Anningqu Experimental Base in Urumqi, Xinjiang in 2022-2023 under three treatments: normal irrigation, rewatering and drought. Plant height and yield per plant were measured. Drought resistance coefficient and average membership function were used for drought resistance identification and cluster analysis. 【Results】 Compared with total irrigation, the measurement indexes of different treatments were significantly different. The plant height and yield per plant decreased by 9.80% and 10.09% in 2022 and 19.35% and 38.07% in 2023, respectively. Drought treatment decreased by 21.05%, 20.61%, 39.82% and 57.16%, respectively. The frequencies of plant height and yield per plant with DC>0.900 under rehydration treatment and drought treatment were 54.42%, 46.26%, 18.71% and 18.37%, respectively. Furthermore, the comprehensive evaluation value of drought resistance of mustard rapeseed was calculated by means of average membership function standardization analysis, and cluster analysis was conducted on the tested varieties based on this value. Re-watering treatment and drought treatment were divided into 5 grades respectively, namely, I (strong resistance), II (resistance), III (medium resistance), IV (more sensitive) and V (sensitive). The regression model of the whole growth period of mustard rapeseed was established, and the rehydration treatment was as follows: Y=0.500X₁+0.499X₂; Drought treatment: Y=0.500X₁+0.500X₂ 【Conclusion】 Six varieties CKH037, CKH087, CKH183, CKH189, CKH209 and CKH282 with strong resistance are selected through the identification of drought resistance.

Key words: Brassica napus; whole reproductive period; drought resistance; drought resistance coefficient; membership function; cluster analysis

摘要：

【目的】干旱是影响新疆芥菜型油菜生产的主要因素之一,鉴定芥菜型油菜全生育期抗旱性,筛选抗旱性强的的芥菜型油菜种质资源,为油菜抗旱育种提供种质资源和理论依据。【方法】以294份芥菜型油菜种质为材料,于2022~2023年在新疆维吾尔自治区农业科学院乌鲁木齐试验站设置正常灌溉、复水、干旱3个处理,测定株高及单株产量,运用抗旱系数、平均隶属函数进行抗旱性鉴定及聚类分析。【结果】不同处理下测定指标差异较大。复水处理使油菜株高和单株产量在2022年和2023年分别下降了9.80%、10.09%和22.48%、39.19%;干旱处理则分别下降了21.05%、20.61%和39.82%、57.16%。不同处理下参试材料的抗旱能力存在显著差异。复水处理及干旱处理下DC>0.900的株高及单株产量的频率分别为54.42%、39.46%、18.71%和18.37%。将复水处理及干旱处理各划分为5个等级,即I级(强抗)、II级(抗)、III级(中抗)、IV级(较敏感)和V级(敏感),并建立芥菜型油菜全生育期多元性回归模型,复水处理:Y=0.500X₁+0.499X₂;干旱处理:Y=0.500X₁+0.500X₂。【结论】筛选出CKH037、CKH087、CKH183、CKH189、CKH209和CKH282强抗品种6份。

关键词: 芥菜型油菜, 全生育期, 抗旱性, 抗旱系数, 隶属函数, 聚类分析

CLC Number:

S565.4

LUO Yutao, HOU Xianfei, GU Yuanguo, LI Qiang, MIAO Haocui, JIA Donghai, DENG Xiaojuan. Evaluation of 294 varieties of brassica rapa cruciferae germplasm for drought resistance throughout the growing season[J]. Xinjiang Agricultural Sciences, 2025, 62(7): 1639-1647.

罗玉涛, 侯献飞, 顾元国, 李强, 苗昊翠, 贾东海, 邓晓娟. 294份芥菜型油菜种质资源全生育期抗旱性鉴定[J]. 新疆农业科学, 2025, 62(7): 1639-1647.

Figures/Tables 7

References 35

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处理方法 Treatment method
灌水 Well watered				复水 Rehydration				干旱 Drought stress
年份 Years	2022年株高	2023年株高	2022年单株产量	2023年单株产量	2022年株高	2023年株高	2022年单株产量	2023年单株产量	2022年株高	2023年株高	2022年单株产量	2023年单株产量
平均值 Mean	129.86	118.95	17.89	18.09	117.14	106.95	13.90	11.00	102.52	94.44	10.79	7.75
标准差 SD	21.31	20.47	8.59	8.73	18.83	17.80	6.20	5.21	19.17	23.13	5.15	4.49
变异系数 CV(%)	16.40	17.21	48.02	48.26	16.07	16.64	44.60	47.36	18.70	24.09	47.73	57.94
下降幅度 Change amplitude (%)	0	0	0	0	9.80	10.09	22.48	39.19	21.05	20.61	39.82	57.16

处理方法 Treatment method
灌水 Well watered				复水 Rehydration				干旱 Drought stress
年份 Years	2022年株高	2023年株高	2022年单株产量	2023年单株产量	2022年株高	2023年株高	2022年单株产量	2023年单株产量	2022年株高	2023年株高	2022年单株产量	2023年单株产量
平均值 Mean	129.86	118.95	17.89	18.09	117.14	106.95	13.90	11.00	102.52	94.44	10.79	7.75
标准差 SD	21.31	20.47	8.59	8.73	18.83	17.80	6.20	5.21	19.17	23.13	5.15	4.49
变异系数 CV(%)	16.40	17.21	48.02	48.26	16.07	16.64	44.60	47.36	18.70	24.09	47.73	57.94
下降幅度 Change amplitude (%)	0	0	0	0	9.80	10.09	22.48	39.19	21.05	20.61	39.82	57.16

参数 Parameter	复水处理Rehydration treatment				干旱处理Drought treatment
参数 Parameter	株高 Plant height (2022)	株高 Plant height (2023)	单株产量 Yield per plant (2022)	单株产量 Yield per plant (2023)	株高 Plant height (2022)	株高 Plant height (2023)	单株产量 Yield per plant (2022)	单株产量 Yield per plant (2023)
最大值 Max	1.548	1.491	1.988	1.995	1.683	1.298	1.825	1.980
最小值Min	0.642	0.622	0.213	0.087	0.445	0.116	0.104	0.024
均值 Average	0.915	0.916	0.934	0.767	0.804	0.814	0.758	0.562
标准差SD	0.12	0.13	0.39	0.37	0.15	0.21	0.37	0.35
变异系数 CV(%)	13.11	14.19	41.76	48.24	18.66	25.80	48.81	62.28

参数 Parameter	复水处理Rehydration treatment				干旱处理Drought treatment
参数 Parameter	株高 Plant height (2022)	株高 Plant height (2023)	单株产量 Yield per plant (2022)	单株产量 Yield per plant (2023)	株高 Plant height (2022)	株高 Plant height (2023)	单株产量 Yield per plant (2022)	单株产量 Yield per plant (2023)
最大值 Max	1.548	1.491	1.988	1.995	1.683	1.298	1.825	1.980
最小值Min	0.642	0.622	0.213	0.087	0.445	0.116	0.104	0.024
均值 Average	0.915	0.916	0.934	0.767	0.804	0.814	0.758	0.562
标准差SD	0.12	0.13	0.39	0.37	0.15	0.21	0.37	0.35
变异系数 CV(%)	13.11	14.19	41.76	48.24	18.66	25.80	48.81	62.28

指标 Indexes	株高(复水) Plant height (rehydration)	单株产量(复水) Yield per plant (rehydration)	株高 (干旱) Plant height (drought)	单株产量 (干旱) Yield per plant (drought)	平均隶属值 (复水) $A\mu ($x) (rehydration)	平均隶属值 (干旱) $A\mu ($x) (drought)
株高(复水) Plant height(rehydration)	1
单株产量(复水) Yield per plant (rehydration)	0.987^**	1
株高(干旱) Plant height(drought)	0.962^**	0.922^**	1
单株产量(干旱) Yield per plant (drought)	0.989^**	0.990^**	0.955^**	1
平均隶属值(复水) $A\mu ($x)(rehydration)	0.996^**	0.985^**	0.957^**	0.991^**	1
平均隶属值(干旱) $A\mu ($x)(drought)	0.993^**	0.979^**	0.965^**	0.987^**	0.993^**	1