Drought resistance evaluation of 11 wild Agropyron cristatum germplasm at germination stage under PEG simulated stress

doi:10.6048/j.issn.1001-4330.2025.03.022

Abstract

Abstract:

【Objective】The germplasm resources of drought tolerant Agropyron cristatum were screened by PEG simulated water stress.【Methods】The seeds of 11 A. cristatum germplasm resources collected were used as the testing materials to evaluate the effects of different Polyethylene glycol (PEG-6000) concentration on six seed germination indexes (relative germination rate, relative germination potential, relative germination index, relative vitality index, relative seedling height and relative root length), and drought resistance of these materials was was comprehensively evaluated by using the membership function method.【Results】The results showed that PEG simulated drought stress had significant effects on the germination stage of 11 A. cristatum germplasm resources, and there were significant differences among the materials, but the indexes showed a decreasing trend with the increase of PEG concentration. Low concentration of drought stress had a certain promotion on germination indexes of A₁, A₈ and A₉, especially on RGR, RGI and RVI. The germination indexes of 11 A. cristatum germplasm resources was inhibited with the increase of PEG stress concentration, and the inhibition effect on RSH and RRL was stronger, when the concentration reached 25%, the A₃ and A₄ indexes were completely inhibited. Correlation analysis showed that there was a significant correlation between the six indicators, and the correlation coefficient was 0.831 to 0.981, RGP has the highest correlation with RGI. The germination indexes of 11 A. cristatum germplasm resources were evaluated by membership function method and with D value range of 0.387,7 to 0.504,5, and the drought resistance of A1 and A9 was better than those of other materials.【Conclusion】There are differences in the indexes of the germplasm resources at the germination stage of 11 A. cristatum germplasm resources under drought stress with the increase of PEG-6000 concentration, and the drought resistance is as follows: A₁>A₉>A₆>A₂>A₇>A₅>A₈>A₁₀>A₁₁>A₄>A₃.

Key words: wild Agropyron cristatum; germplasm resources; seed germination; drought resistance

摘要：

【目的】通过PEG模拟水分胁迫筛选出耐旱冰草种质资源。【方法】收集11份野生冰草为试验材料,分析不同浓度的聚乙二醇(PEG-6000)模拟干旱胁迫对其种子相对发芽势(PGR)、相对发芽率(RGP)、相对发芽指数(RGI)、相对活力指数(RVI)、相对苗高(RSH)和相对根长(RRL)的影响,并采用隶属函数法对抗旱性进行综合评价。【结果】PEG模拟干旱胁迫对11份野生冰草种质资源萌发期各指标均有影响,且材料间差异明显,但各项指标均随着PEG浓度的增加呈下降趋势。低浓度(5%)的干旱胁迫对A₁、A₈和A₉各项萌发指标具有一定的促进作用,尤其是对RGR,RGI和RVI。随着PEG处理浓度的增加,11份野生冰草种质资源的各项萌发指标均受到了不同程度的抑制,尤其是对RSH和RRL抑制作用较强,当浓度达到25%时,A₃和A₄各项指标均被完全抑制。6个萌发指标间存在极显著正相关关系,相关系数在0.831~0.981,RGP与RGI的相关性最高。11份野生冰草种质资源的D值范围在0.387 7~0.504 5,其中,A₁和A₉的抗旱性优于其他材料。【结论】随着PEG-6000 浓度的增加,11份野生冰草种质资源萌发期各个指标均表现出明显的差异,其抗旱性强弱依次为A₁>A₉>A₆>A₂>A₇>A₅>A₈>A₁₀>A₁₁>A₄>A₃。

关键词: 野生冰草, 种质资源, 种子萌发, 抗旱性

CLC Number:

S543

YI Fengyan, SUN Shixian, GUO Chengyu, YAN Xiaohong, FANG Yongyu, YANG Ding, DING Haijun. Drought resistance evaluation of 11 wild Agropyron cristatum germplasm at germination stage under PEG simulated stress[J]. Xinjiang Agricultural Sciences, 2025, 62(3): 723-731.

伊风艳, 孙世贤, 郭呈宇, 闫晓红, 房永雨, 杨鼎, 丁海君. PEG模拟干旱胁迫下11份野生冰草种质资源萌发期抗旱性评价[J]. 新疆农业科学, 2025, 62(3): 723-731.

Figures/Tables 6

References 25

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编号 No	采集地点 Collection site	海拔 Altitude(km)	生境 Habitat	土壤类型 Soil type	千粒重 1000-grain weight(g)
A₁	包头市达茂旗	1 630	天然草地	沙壤土	1.796
A₂	包头市达茂旗	1 584	天然草地	沙壤土	1.810
A₃	包头市大青山	1 358	-	壤土	2.311
A₄	锡林浩特市白音锡勒牧场	1 100	天然草地	沙壤土	1.880
A₅	锡林浩特市白音锡勒牧场	1 235	天然草地	沙壤土	2.252
A₆	锡林浩特市	1 010	天然草地	沙壤土	1.760
A₇	锡林浩特市白音锡勒牧场	1 278	天然草地	壤土	1.851
A₈	锡林浩特市朝克乌拉苏木	919	天然草地	沙壤土	1.795
A₉	锡林浩特市毛登牧场	1 125	天然草地	沙壤土	1.862
A₁₀	锡林郭勒盟正镶白旗	1 374	天然草地	壤土	1.655
A₁₁	锡林郭勒盟正蓝旗	1291	沙地	沙土	1.963

编号 No	采集地点 Collection site	海拔 Altitude(km)	生境 Habitat	土壤类型 Soil type	千粒重 1000-grain weight(g)
A₁	包头市达茂旗	1 630	天然草地	沙壤土	1.796
A₂	包头市达茂旗	1 584	天然草地	沙壤土	1.810
A₃	包头市大青山	1 358	-	壤土	2.311
A₄	锡林浩特市白音锡勒牧场	1 100	天然草地	沙壤土	1.880
A₅	锡林浩特市白音锡勒牧场	1 235	天然草地	沙壤土	2.252
A₆	锡林浩特市	1 010	天然草地	沙壤土	1.760
A₇	锡林浩特市白音锡勒牧场	1 278	天然草地	壤土	1.851
A₈	锡林浩特市朝克乌拉苏木	919	天然草地	沙壤土	1.795
A₉	锡林浩特市毛登牧场	1 125	天然草地	沙壤土	1.862
A₁₀	锡林郭勒盟正镶白旗	1 374	天然草地	壤土	1.655
A₁₁	锡林郭勒盟正蓝旗	1291	沙地	沙土	1.963

编号 No	相对发芽率RGP					相对发芽势RGR
编号 No	5%	10%	15%	20%	25%	5%	10%	15%	20%	25%
A₁	106.73^Aa	84.06^Cb	62.20^Cc	38.63^Bd	7.56^Be	116.42^Ca	91.79^DEb	63.24^Eb	6.13^Fc	0.00^Ad
A₂	92.37^CDa	79.68^CDb	70.34^Bc	25.65^Dd	9.32^ABe	138.38^Aa	126.51^Bb	94.19^ABb	17.68^Ec	0.00^Ad
A₃	78.30^Ea	61.01^Fb	36.77^Dc	16.07^Ed	0.00^Ee	97.22^Da	75.56^FGb	59.44^Ec	9.44^Fd	0.00^Ae
A₄	100.74^Ba	92.54^Bb	81.36^Ac	18.65^Ed	0.00^Ee	98.85^Da	78.26^Fb	58.95^Ec	7.23^Fd	0.00^Ae
A₅	97.04^BCa	84.32^Cb	72.41^Bc	34.33^Cd	7.46^Be	105.56^Da	96.39^CDb	85.48^Cc	25.54^CDd	0.00^Ae
A₆	100.90^Ba	78.45^Eb	62.96^Cc	39.65^Bd	9.49^ABe	126.52^Ba	123.48^Ba	100.25^Ab	53.03^Ac	0.00^Ad
A₇	88.19^Da	83.48^Cb	71.67^Bc	46.46^Ad	8.66^ABe	103.92^Da	100.11^Ca	88.45^BCb	34.53^Bc	0.00^Ae
A₈	102.31^ABa	80.16^CDb	72.55^Bc	39.71^Bd	4.58^Ce	128.43^Ba	86.82^Eb	83.01^Cb	50.87^Ac	0.00^Ad
A₉	104.48^Aa	96.44^Ab	74.11^Bc	36.60^BCd	2.68^De	141.90^Aa	135.64^Aa	83.42^Cb	20.88^DEc	0.00^Ad
A₁₀	106.98^Aa	100.00^Ab	86.19^Ac	39.60^Bd	9.89^Ae	122.55^BCa	100.00^Cb	73.41^Dc	22.43^DEd	0.00^Ae
A₁₁	75.84^Ea	62.62^Fb	40.68^Dc	31.90^Cd	7.67^Be	64.90^Ea	63.43^Ha	41.16^Fb	29.29^Cc	0.00^Ad

编号 No	相对发芽率RGP					相对发芽势RGR
编号 No	5%	10%	15%	20%	25%	5%	10%	15%	20%	25%
A₁	106.73^Aa	84.06^Cb	62.20^Cc	38.63^Bd	7.56^Be	116.42^Ca	91.79^DEb	63.24^Eb	6.13^Fc	0.00^Ad
A₂	92.37^CDa	79.68^CDb	70.34^Bc	25.65^Dd	9.32^ABe	138.38^Aa	126.51^Bb	94.19^ABb	17.68^Ec	0.00^Ad
A₃	78.30^Ea	61.01^Fb	36.77^Dc	16.07^Ed	0.00^Ee	97.22^Da	75.56^FGb	59.44^Ec	9.44^Fd	0.00^Ae
A₄	100.74^Ba	92.54^Bb	81.36^Ac	18.65^Ed	0.00^Ee	98.85^Da	78.26^Fb	58.95^Ec	7.23^Fd	0.00^Ae
A₅	97.04^BCa	84.32^Cb	72.41^Bc	34.33^Cd	7.46^Be	105.56^Da	96.39^CDb	85.48^Cc	25.54^CDd	0.00^Ae
A₆	100.90^Ba	78.45^Eb	62.96^Cc	39.65^Bd	9.49^ABe	126.52^Ba	123.48^Ba	100.25^Ab	53.03^Ac	0.00^Ad
A₇	88.19^Da	83.48^Cb	71.67^Bc	46.46^Ad	8.66^ABe	103.92^Da	100.11^Ca	88.45^BCb	34.53^Bc	0.00^Ae
A₈	102.31^ABa	80.16^CDb	72.55^Bc	39.71^Bd	4.58^Ce	128.43^Ba	86.82^Eb	83.01^Cb	50.87^Ac	0.00^Ad
A₉	104.48^Aa	96.44^Ab	74.11^Bc	36.60^BCd	2.68^De	141.90^Aa	135.64^Aa	83.42^Cb	20.88^DEc	0.00^Ad
A₁₀	106.98^Aa	100.00^Ab	86.19^Ac	39.60^Bd	9.89^Ae	122.55^BCa	100.00^Cb	73.41^Dc	22.43^DEd	0.00^Ae
A₁₁	75.84^Ea	62.62^Fb	40.68^Dc	31.90^Cd	7.67^Be	64.90^Ea	63.43^Ha	41.16^Fb	29.29^Cc	0.00^Ad

编号 No	相对发芽指数RGI					相对活力指数RVI
编号 No	5%	10%	15%	20%	25%	5%	10%	15%	20%	25%
A₁	112.50^BCa	86.76^CDb	58.55^Dc	24.19^EFd	4.12^De	121.27^Aa	87.99^Bb	47.95^CDc	12.96^EFd	1.28^Be
A₂	109.62^Da	98.81^Bb	72.27^BCc	22.28^Fd	5.68^ABe	100.18^Ca	85.43^BCb	46.20^Dc	12.67^EFd	0.45^Ee
A₃	86.20^Fa	62.50^Eb	37.01^Cc	12.99^Gd	0.00^Ge	88.49^Da	56.94^EFb	26.82^Ec	3.50^Gd	0.00^Gd
A₄	101.68^Ea	90.07^Cb	72.15^BCc	10.83^Gd	0.00^Ge	104.11^Ca	83.14^BCb	45.89^Dc	4.57^Gd	0.00^Ge
A₅	110.47^Da	90.74^Cb	76.07^Bc	26.47^EFd	4.85^BCDe	101.72^Ca	75.48^Db	51.83^BCc	11.41^EFd	0.56^Ee
A₆	117.19^ABa	105.88^Ab	74.40^CBc	38.08^ABd	5.12^BCe	106.33^BCa	94.95^Ab	59.86^Ac	26.82^Ad	0.98^Ce
A₇	87.84^Fa	82.01^Db	72.84^BCc	32.62^CDd	4.42^CDe	78.97^Ea	58.48^Eb	45.33^Dc	16.38^CDd	0.64^DEe
A₈	121.93^Aa	80.56^Db	68.19^Cc	35.48^BCd	2.46^Ee	127.82^Aa	75.72^Db	52.53^Bc	18.45^Bd	0.37^EFe
A₉	109.44^Da	107.40^Aa	75.92^Bb	24.90^EFc	1.54^Fd	122.83^Aa	98.34^Ab	59.03^Ac	11.06^Fd	0.19^FGe
A₁₀	112.95^BCa	106.45^Ab	90.33^Ac	41.18^Ad	6.21^Ae	112.75^Ba	95.83^Ab	44.31^Dc	18.97^BCd	1.59^Ae
A₁₁	72.63^Ga	65.15^Eb	44.13^Ec	28.59^DEd	4.81^BCDe	70.47^Fa	51.98^Fb	28.18^Ec	14.62^DEd	0.85^CDe