Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

doi:10.6048/j.issn.1001-4330.2023.11.007

Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (11): 2653-2660.DOI: 10.6048/j.issn.1001-4330.2023.11.007

• Crop Genetics and Breeding · Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles

Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

SHAN Ying¹(), LI Yue¹, XU Min¹, LIU Yanzhen¹, LI Ran², CHEN Jieyin², WANG Zisheng¹(), ZHU He¹()

1. Cash Crop Institute, Liaoning Academy of Agricultural Sciences, Liaoyang Liaoning 111000, China
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2023-01-25 Online:2023-11-20 Published:2023-12-07
Correspondence author: ZHU He(1984-),male,associate research fellow,research direction: utilization and innovation of cotton germplasm resources,(E-mail)zhuhe19840204@163.com;WANG Zisheng(1965-),male,research fellow,doctor,research direction: cotton breeding, cultivation and promotion,(E-mail)wangzisheng6666@126.com
Supported by:
Liaohe Cotton Experiment Station, CARS(CARS-15-31);State Key Laboratory of Cotton Biology Open Fund(CB2022A25)

PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价

单莹¹(), 李越¹, 徐敏¹, 刘艳珍¹, 李冉², 陈捷胤², 王子胜¹(), 朱鹤¹()

1.辽宁省农业科学院经济作物研究所,辽宁辽阳 111000
2.中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京 100193

通讯作者: 朱鹤(1984-),男,辽宁辽阳人,副研究员,研究方向为棉花种质资源,(E-mail)zhuhe19840204@163.com;王子胜(1965-),男,辽宁朝阳人,研究员,博士,研究方向为棉花育种栽培,(E-mail)wangzisheng6666@126.com
作者简介:单莹(1981-),女,辽宁辽阳人,助理研究员,硕士,研究方向为棉花遗传育种,(E-mail)shanying_sy@163.com
基金资助:
国家现代农业产业技术体系棉花产业技术体系辽河综合试验站(CARS-15-31);棉花生物学国家重点实验室开放课题基金(CB2022A25)

Abstract

Abstract:

【Objective】 To study the drought resistance at seed germination stage of six Liaomian cotton varieties under PEG simulated drought stress.【Methods】 The seeds of six Liaomian varieties were used as the testing materials to study drought resistance at seed germination stage under different concentrations of PEG-6000 (0, 2.5%, 5.0%, 7.5% and 10.0%). Germination energy, germination rate, germination index, germination drought resistance index, hypocotyl length, root length, seedling fresh weight and root fresh weight were measured under different drought stress.【Results】 The germination of cotton seeds was inhibited to different degrees, and the germination energy, germination rate and germination drought resistance index were decreased with the increase of PEG concentrations. Seedling growth index and root growth index were significantly inhibited under 2.5% PEG stress. 5.0% PEG stress promoted root length growth of all materials. The inhibition degrees of each index under PEG stress above 5.0% concentration were gradually increased with the increase of stress.【Conclusion】 The six Liaomian cotton varieties were sorted according to their drought resistance as follows: Liaomian 10>Liaomian 23>Liaomian 45>Liaomian 53>Liaomian 49>Liaomian 34.

Key words: cotton; PEG stress; germination stage; drought resistance

摘要：

【目的】 评价PEG模拟干旱胁迫下6个辽棉品种种子萌发期的抗旱性。【方法】 以6个辽棉系列审定品种为材料,采用不同浓度(0、2.5%、5.0%、7.5%、10.0%)的PEG-6000模拟干旱胁迫条件,设置萌发期抗旱试验,测定各材料对照和不同干旱胁迫下的发芽势、发芽率、萌发指数、萌发抗旱指数、下胚轴长、主根长、芽苗鲜重和根鲜重。【结果】 随PEG干旱胁迫程度的增加,棉花种子萌发均受到不同程度抑制,发芽势、发芽率、萌发抗旱指数随胁迫程度的加重而降低,幼苗生长指标和根系生长指标在2.5% PEG胁迫受抑制显著,5.0% PEG胁迫促进了各材料的根长生长,各指标在5.0% 浓度以上PEG胁迫时受抑制程度随胁迫增加逐渐增强。【结论】 6个辽棉系列品种的抗旱性由强至弱的顺序为辽棉10号>辽棉23号>辽棉45号>辽棉53号>辽棉49号>辽棉34号。

关键词: 棉花, PEG胁迫, 萌发期, 抗旱性

CLC Number:

S562

SHAN Ying, LI Yue, XU Min, LIU Yanzhen, LI Ran, CHEN Jieyin, WANG Zisheng, ZHU He. Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress[J]. Xinjiang Agricultural Sciences, 2023, 60(11): 2653-2660.

单莹, 李越, 徐敏, 刘艳珍, 李冉, 陈捷胤, 王子胜, 朱鹤. PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价[J]. 新疆农业科学, 2023, 60(11): 2653-2660.

Figures/Tables 10

References 18

[1]	杨利艳, 杨小兰, 朱满喜, 等. 干旱胁迫对藜麦种子萌发及幼苗生理特性的影响[J]. 种子, 2020, 39(9):36-40.
	YANG Liyan, YANG Xiaolan, ZHU Manxi, et al. Effects of drought stress on seed germination and seedling physiological characteristics of quinoa[J]. Seed, 2020, 39(9):36-40.
[2]	周芳, 曹国璠, 杨娟, 等. 20个玉米品种发芽期抗旱性鉴定与评价[J]. 种子, 2020, 39(12):73-79.
	ZHOU Fang, CAO Guofan, YANG Juan, et al. Identification and evaluation of drought stress of twenty maize varieties in germination period[J]. Seed, 2020, 39(12):73-79.
[3]	张卫杰, 韩松, 吉庆勋, 等. 种子处理对PEG-6000模拟渗透胁迫下棉花发芽的影响[J]. 江苏农业科学, 2020, 48(2):91-96.
	ZHANG Weijie, HAN Song, JI Qingxun, et al. Effect of seed treatment on cotton germination under PEG-6000 simulated osmotic stress[J]. Jiangsu Agricultural Sciences, 2020, 48(2):91-96.
[4]	王延琴, 杨伟华, 许红霞, 等. 水分胁迫对棉花种子萌发的影响[J]. 棉花学报, 2009, 21(1):73-76.
	WANG Yanqin, YANG Weihua, XU Hongxia, et al. Effect of water stress on germination of cotton seeds[J]. Cotton Science, 2009, 21(1):73-76.
[5]	李志博, 魏亦农. 北疆主栽棉花种子对渗透胁迫的响应及其萌发力差异评价[J]. 种子, 2010, 29(7):1-4.
	LI Zhibo, WEI Yinong. Germination response and drought-resistance evaluation of cotton seed to osmotic stress in northern Xinjiang[J]. Seed, 2010, 29(7):1-4.
[6]	姚庆, 秦培友, 苗昊翠, 等. PEG模拟干旱胁迫下藜麦萌发期抗旱性评价[J]. 新疆农业科学, 2019, 56(9):1588-1596. DOI
	YAO Qing, QIN Peiyou, MIAO Haocui, et al. Drought resistance evaluation of quinoa during germination under PEG simulated drought stress[J]. Xinjiang Agricultural Sciences, 2019, 56(9):1588-1596. DOI
[7]	李瑶, 隋晓青, 郝裕辉, 等. 干旱胁迫下新疆7份野生披碱草种质材料萌发特性与抗旱性评价[J]. 新疆农业科学, 2020, 57(5):958-966. DOI
	LI Yao, SUI Xiaoqing, HAO Yuhui, et al. Germination characteristics and drought resistance evaluation of seven wild Elymus L. germplasms in Xinjiang under drought stress[J]. Xinjiang Agricultural Sciences, 2020, 57(5):958-966. DOI
[8]	孙瑶, 杨晓杰, 薄晓培, 等. 棉花芽期的抗旱性鉴定及综合评价[J]. 种子, 2020, 39(10):16-22.
	SUN Yao, YANG Xiaojie, BO Xiaopei, et al. Drought-resistance identification and comprehensive evaluation of cotton at bud seedling stage[J]. Seed, 2020, 39(10):16-22.
[9]	季杨, 张新全, 彭燕, 等. 鸭茅种子萌发对渗透胁迫响应与耐旱性评价[J]. 草地学报, 2013, 21(4):737-743. DOI
	JI Yang, ZHANG Xinquan, PENG Yan, et al. Seed germination response to osmotic stress and tolerance evaluation of orchardgrass[J]. Acta Agrectir Sinaca, 2013, 21(4):737-743.
[10]	宋国英, 刘国一, 边巴卓玛. 模拟干旱胁迫下7个黑青稞品种的萌发特性与抗旱性评价[J]. 江苏农业科学, 2021, 49(16):84-88.
	SONG Guoying, LIU Guoyi, Bianbazhuoma. Evaluation of germination characteristics and drought resistance of 7 dark barley cultivars under simulated drought stress[J]. Jiangsu Agricultural Sciences, 2021, 49(16):84-88.
[11]	江绪文, 李贺勤, 王晓琨, 等. PEG-6000模拟干旱胁迫对5个玉米品种种子萌发及活力的影响[J]. 种子, 2015, 34(5):5-8.
	JIANG Xuwen, LI Heqin, WANG Xiaokun, et al. Effects of PEG-6000 simulated drought stress on seed germination and vigor of five maize varieties[J]. Seed, 2015, 34(5):5-8.
[12]	石汝杰, 胡廷章. 渗透胁迫对4个玉米品种种子萌发及幼苗生长的影响[J]. 种子, 2009, 28(7):85-87.
	SHI Rujie, HU Tingzhang. Effects of permeating stress on seeds germination and seedlings growth of four maize varieties[J]. Seed, 2009, 28(7):85-87.
[13]	赵璞, 温之雨, 董文琦, 等. PEG-6000模拟干旱胁迫对8个玉米种质种子萌发及活力的影响[J]. 种子, 2020, 39(10):48-52.
	ZHAO Pu, WEN Zhiyu, DONG Wenqi, et al. Effects of PEG-6000 simulated drought stress on seed germination and seed vigor of 8 maize germplasm[J]. Seed, 2020, 39(10):48-52.
[14]	牛冰洁, 田超, 王永新, 等. 6个燕麦品种种子萌发期抗旱性比较[J]. 种子, 2021, 40(1):73-78.
	NIU Bingjie, TIAN Chao, WANG Yongxin, et al. Comparison of drought resistance of six oat varieties during seed germination[J]. Seed, 2021, 40(1):73-78.
[15]	丁国华, 白良明, 曹良子, 等. 干旱胁迫对寒地水稻不同种质资源萌发特性及幼苗生长的影响[J]. 种子, 2020, 39(11):38-43.
	DING Guohua, BAI Liangming, CAO Liangzi, et al. Effects of drought stress on germination characteristics and seedling growth of different rice germplasm resources in cold region[J]. Seed, 2020, 39(11):38-43.
[16]	王俊娟, 叶武威, 王德龙, 等. PEG胁迫条件下41份陆地棉种质资源萌发特性研究及抗旱性综合评[J]. 植物遗传资源学报, 2011, 12(6):840-846. DOI
	WANG Junjuan, YE Wuwei, WANG Delong, et al. Germination characteristics and comprehensive evaluation of drought stress of 41 accessions of cotton germplasm at seed germination stage under PEG6000 stress[J]. Journal of Plant Genetic Resources, 2011, 12(6):840-846.
[17]	王鹏, 侯思宇, 温宏伟, 等. 干旱胁迫对滞绿大豆种子萌发的影响及芽期抗旱性评价[J]. 大豆科学, 2021, 40(1):68-74.
	WANG Peng, HOU Siyu, WEN Hongwei, et al. Effects of drought stress on seed germination and evaluation of drought resistance in bud stage of stay-green soybean[J]. Soybean Science, 2021, 40(1):68-74.
[18]	胡根海, 董娜, 晁毛妮, 等. PEG模拟干旱胁迫对不同抗逆性棉花的生理特性的影响[J]. 干旱地区农业研究, 2017, 35(5):223-228.
	HU Genhai, DONG Na, CHAO Maoni, et al. Effects of drought stress simulated by PEG6000 on physiological characteristics of different upland cotton[J]. Agricultural Research in the Arid Areas, 2017, 35(5):223-228.

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.0±2.0^a	95.3±2.3^a	88.0±2.0^a	95.3±3.1^a	96.0±3.5^a	96.0±4.0^a
2.5	85.3±2.3^b	90.0±4.0^ab	82.0±3.5^b	90.7±3.1^a	81.3±4.6^b	75.3±5.0^b
5.0	66.7±3.1^c	75.3±1.2^c	48.0±2.0^c	81.3±2.3^b	70.0±3.5^c	54.7±5.0^c
7.5	65.3±3.1^c	84.7±4.2^b	38.0±2.0^d	78.7±3.1^b	23.3±3.1^d	50.7±1.2^c
10.0	50.0±3.5^d	56.7±4.2^d	26.0±2.0^e	42.0±4.0^c	7.3±2.3^e	28.7±3.1^d

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.0±2.0^a	95.3±2.3^a	88.0±2.0^a	95.3±3.1^a	96.0±3.5^a	96.0±4.0^a
2.5	85.3±2.3^b	90.0±4.0^ab	82.0±3.5^b	90.7±3.1^a	81.3±4.6^b	75.3±5.0^b
5.0	66.7±3.1^c	75.3±1.2^c	48.0±2.0^c	81.3±2.3^b	70.0±3.5^c	54.7±5.0^c
7.5	65.3±3.1^c	84.7±4.2^b	38.0±2.0^d	78.7±3.1^b	23.3±3.1^d	50.7±1.2^c
10.0	50.0±3.5^d	56.7±4.2^d	26.0±2.0^e	42.0±4.0^c	7.3±2.3^e	28.7±3.1^d

品种Varieties	R²	回归方程Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.953 3	Y=92.667-4.16X	3.04
辽棉23号Liaomian 23	0.740 9	Y=96.933-3.306 7X	5.12
辽棉34号Liaomian 34	0.944 8	Y=90-6.72X	1.49
辽棉45号Liaomian 45	0.796 6	Y=101.33-4.746 7X	4.49
辽棉49号Liaomian 49	0.943 1	Y=102.67-9.413 3X	2.41
辽棉53号Liaomian 53	0.968	Y=92.933-6.373 3X	2.03

品种Varieties	R²	回归方程Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.953 3	Y=92.667-4.16X	3.04
辽棉23号Liaomian 23	0.740 9	Y=96.933-3.306 7X	5.12
辽棉34号Liaomian 34	0.944 8	Y=90-6.72X	1.49
辽棉45号Liaomian 45	0.796 6	Y=101.33-4.746 7X	4.49
辽棉49号Liaomian 49	0.943 1	Y=102.67-9.413 3X	2.41
辽棉53号Liaomian 53	0.968	Y=92.933-6.373 3X	2.03

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
CK	92.7±1.2^a	98.0±2.0^a	94.0±2.0^a	97.3±3.1^a	98.7±1.2^a	97.3±2.3^a
2.5	89.3±4.2^ab	94.7±3.1^ab	85.3±2.3^b	94.0±2.0^ab	92.0±2.0^b	80.0±2.0^b
5.0	88.7±4.2^ab	93.3±3.1^ab	81.3±3.1^b	88.0±3.5^c	87.3±3.1^b	80.0±4.0^b
7.5	83.3±3.1^b	91.3±5.0^b	62.7±3.1^c	88.7±3.1^bc	68.7±4.6^c	56.0±3.5^c
10.0	72.7±3.1^c	80.0±2.0^c	60.7±3.1^c	64.7±3.1^d	28.0±2.0^c	54.7±4.2^c

Drought resistance evaluation of liaomian cotton varieties at germination stage under PEG simulated drought stress

PEG模拟干旱胁迫下辽棉系列品种种子萌发期抗旱性评价

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 18

Related Articles 15

Recommended Articles

Metrics

品种Varieties	R²	回归方程 Regression equation	Y=80,X值 Y=80,X value
辽棉10号Liaomian 10	0.862 5	Y=94.533-1.84X	7.90
辽棉23号Liaomian 23	0.823 3	Y=99.333-1.573 3X	12.29
辽棉34号Liaomian 34	0.939 7	Y=94.667-3.573 3X	4.10
辽棉45号Liaomian 45	0.759 8	Y=100.67-2.826 7X	7.31
辽棉49号Liaomian 49	0.834 2	Y=107.87-6.586 7X	4.23
辽棉53号Liaomian 53	0.910 1	Y=95.467-4.373 3X	3.54

品种 Varieties	隶属函数值 Subordinate function value							平均值 Average	抗旱排名 Sort
品种 Varieties	相对发芽势 RGE	相对发芽率 RGR	相对下胚轴长 RHL	相对根长 RRL	相对苗鲜重 RSFW	相对根鲜重 RRFW	萌发抗旱指数 GDRI	平均值 Average	抗旱排名 Sort
辽棉10号 Liaomian 10	0.77	0.96	0.67	0.51	0.72	0.80	0.57	0.715	1
辽棉23号 Liaomian 23	0.94	0.99	0.30	0.97	0.24	0.54	0.97	0.707	2
辽棉34号 Liaomian 34	0.40	0.46	0.53	0.25	0.28	0.38	0.20	0.356	6
辽棉45号 Liaomian 45	0.90	0.82	0.20	0.76	0.40	0.59	0.89	0.652	3
辽棉49号 Liaomian 49	0.24	0.39	0.22	0.38	0.56	0.59	0.21	0.373	5
辽棉53号 Liaomian 53	0.24	0.13	0.74	0.66	0.70	0.25	0.16	0.411	4

[1]	ZHOU Xin, LIU Xuanfeng, JIANG Yuhan, ZHANG Haichun, YANG Yuxin, Yeerbdati Tiemuer, JIANG Yongxin, ZHANG Li. Current situation and development proposal of mechanized recovery and resource utilization of used mulch film in cotton fields in Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 131-141.
[2]	MIAO Hongping, WANG Xiaowei, TIAN Conghua, LI Zhi, ZHANG Yuxin, DAI Junsheng. Evolution characteristics and driving factors of cotton production and distribution in Tarim River basin [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 217-226.
[3]	WANG Junduo, CUI Yujiang, LIANG Yajun, GONG Zhaolong, ZHENG Junyun, LI Xueyuan. Xinjiang cotton production advantageous regional layout scheme [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 60-69.
[4]	ZHENG Juyun, GONG Zhaolong, LIANG Yajun, GENG Shiwei, SUN Fenglei, YANG ni, LI Xueyuan, WANG Junduo. Key technology model of machine-picked cotton production in Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 70-74.
[5]	LI Jie, LIU Jia, WANG Liang, ZHANG Na, YANG Yanlong, ZHENG Zipiao, WEI Xin, WANG Meng, ZHOU Zixin, YANG Ni, GONG Zhaolong, HOU Xianfei, HUANG Qixiu, Abudukadier kuerban, ZHANG Jipeng, CHANG Pengzhong. Current situation of transformation and application of scientific and technological achievements of "cotton, oil and sugar" [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 89-94.
[6]	BIAN Qingyong, FU Yanbo, QI Tong, HUANG Jian, PU Shenghai, MENG Ajing, Halihashi Yibati. Study on influencing factors of cotton emergence and protection measures in saline-alkali land in southern Xinjiang [J]. Xinjiang Agricultural Sciences, 2024, 61(S1): 95-100.
[7]	LI Yongtai, GAO Axiang, LI Yanjun, ZHANG Xinyu. Effects of defoliants on the physiological characteristics of cotton varieties with different sensitivities [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2094-2102.
[8]	ZHANG Zehua, YE Hanchun, WANG Zhenhua, LI Wenhao, LI Haiqiang, LIU Jian. Effects of equal nitrogen applied with urease inhibitor on cotton growth, yield, and quality under mulched drip irrigation [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2103-2111.
[9]	CHEN Ruijie, LUO Linyi, RUAN Xiangyang, YE Jun. Effects of humic acid on soil nutrients, cotton yield and quality in cotton fields under drip irrigation [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2112-2121.
[10]	HUANG Boxuan, LI Pengcheng, ZHENG Cangsong, SUN Miao, SHAO Jingjing, FENG Weina, PANG Chaoyou, XU Wenxiu, DONG Helin. Effects of different nitrogen inhibitors on growth, nitrogen utilization and yield of cotton [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2122-2131.
[11]	WANG Chao, XU Wenxiu, LI Pengcheng, ZHENG Cangsong, SUN Miao, FENG Weina, SHAO Jingjing, DONG Helin. Response of cotton seedling growth and development to soil available potassium levels [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2132-2139.
[12]	ZENG Wanying, GENG Hongwei, CHENG Yukun, LI Sizhong, QIAN Songting, GAO Weishi, ZHANG Liming. Comprehensive evaluation of drought resistance during the rapid growth stage of sugar beet cultivars [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2140-2151.
[13]	ZHANG Tingjun, LI Zihui, CUI Yujiang, SUN Xiaogui, CHEN Fang. Effects of microbial agents on cotton growth and soil physico-chemical properties [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2269-2276.
[14]	LI Jinyao, XU Guiqing, WANG Lisheng, LU Ping, SHI Dongfang, ZHENG Weihua. Study on the effect of N fertilization on drought resistance of Calligonum caput-medusae seedlings [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2330-2340.
[15]	DONG Zhiduo, XU Fei, FU Qiuping, HUANG Jian, QI Tong, MENG Ajing, FU Yanbo, Kaisaier Kuerban. Effects of different types of salt and alkali stress on cotton seed germination [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1831-1844.

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
2.5	0.88^a	0.93^a	0.83^a	0.94^a	0.84^a	0.79^a
5.0	0.72^b	0.84^b	0.58^b	0.85^ab	0.72^b	0.58^b
7.5	0.66^b	0.84^b	0.43^c	0.82^b	0.40^c	0.53^b
10.0	0.43^c	0.67^c	0.38^c	0.46^c	0.13^d	0.32^c

浓度 Concentrations (%)	品种Varieties
浓度 Concentrations (%)	辽棉10号 Liaomian 10	辽棉23号 Liaomian 23	辽棉34号 Liaomian 34	辽棉45号 Liaomian 45	辽棉49号 Liaomian 49	辽棉53号 Liaomian 53
2.5	0.88^a	0.93^a	0.83^a	0.94^a	0.84^a	0.79^a
5.0	0.72^b	0.84^b	0.58^b	0.85^ab	0.72^b	0.58^b
7.5	0.66^b	0.84^b	0.43^c	0.82^b	0.40^c	0.53^b
10.0	0.43^c	0.67^c	0.38^c	0.46^c	0.13^d	0.32^c