QTL mapping of processing quality traits on RIL population in rice

doi:10.6048/j.issn.1001-4330.2023.10.010

Abstract

Abstract:

【Objective】 To explore the fundamental factors related to the quality of processed rice. 【Methods】 A RIL population F₇ with 243 families constructed by 13JZ-44/13JZ-19 was used as the test material, and the phenotypic data of brown rate, milled rice rate and head milled rice rate were collected from 2019 to 2021, and QTL related to processing quality was analyzed.【Results】 The results showed that 9 QTLs related to processing quality traits were detected, among which 2 QTLs of brown rice percentage were located on chromosome 4 and 11, respectively, with phenotypic contribution rates of 18.68% and 75.32%. The three QTLs of milled rice rate were located on chromosomes 1, 2 and 5, respectively, with the contribution rates of 4.35%, 0.75% and 16.21%. The four QTLs of head rice rate were located on chromosomes 5, 6 and 8, respectively, with the contribution rates of 10.76%, 5.86%, 5.42% and 3.66%.【Conclusion】 qHR-6-1, qHR-6-2 and qBR-11 are newly discovered Loci for controlling brown rice rate and head rice rate, qHR-6-1 and qHR-6-2 have slight effect on head rice rate. And qBR-11 is a new major QTL for controlling brown rice rate.

Key words: rice; RIL population; processing quality; traits; QTL analysis

摘要：

【目的】 研究挖掘水稻加工品质性状相关基因位点。【方法】 以13HJZ-44/13HJZ-19构建的包括243个家系的RIL群体F₇代为材料,于2019~2021年采集稻米出糙率、精米率和整精米率表型数据,并分析加工品质相关性状QTL。【结果】 共检测到加工品质性状相关QTL 9个,其中糙米率的2个QTL分别位于4号和11号染色体上,表型贡献率为18.68%和75.32%;精米率3个QTL分别位于1号、2号和5号染色体上,贡献率为4.35%、0.75%和16.21%;整精米率4个QTL分别位于5号、6号和8号染色体上,贡献率为10.76%、5.86%、5.42%和3.66%。【结论】 qHR-6-1、qHR-6-2和qBR-11是控制糙米率和整精米率位点,其中qHR-6-1、qHR-6-2对整精米率有微效性,qBR-11是一个新的控制糙米率主效QTL。

关键词: 水稻, 重组自交系, 加工品质, 性状, QTL分析

CLC Number:

S511

CHEN Li, MA Jing, ZHU Zhiming, LIU Wei, SUN Jianchang. QTL mapping of processing quality traits on RIL population in rice[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2419-2425.

陈丽, 马静, 朱志明, 刘炜, 孙建昌. 基于水稻RIL群体的加工品质性状QTL分析[J]. 新疆农业科学, 2023, 60(10): 2419-2425.

Figures/Tables 5

References 30

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性状 Trait	亲本Parent			重组自交系群体RIL population
性状 Trait	13HJZ -44	13HJZ -19	差值 Difference	均值 Mean	标准差 SD	最大值 Max	最小值 Min	变异系数 Coefficient of variation(%)	峰度 Kurtosis	偏度 Skewness
出糙率 Brown rice rate (%)	83.68	84.15	0.47	84.07	1.72	90.00	70.00	48.91	48.96	-6.122
精米率 Milled rice rate (%)	70.25	73.25	2.99	72.57	2.81	86.00	57.00	25.83	8.239	-1.275
整精米率 Head rice rate (%)	41.27	61.02	19.75	54.10	5.86	66.00	37.00	9.23	-0.389	-0.48

性状 Trait	亲本Parent			重组自交系群体RIL population
性状 Trait	13HJZ -44	13HJZ -19	差值 Difference	均值 Mean	标准差 SD	最大值 Max	最小值 Min	变异系数 Coefficient of variation(%)	峰度 Kurtosis	偏度 Skewness
出糙率 Brown rice rate (%)	83.68	84.15	0.47	84.07	1.72	90.00	70.00	48.91	48.96	-6.122
精米率 Milled rice rate (%)	70.25	73.25	2.99	72.57	2.81	86.00	57.00	25.83	8.239	-1.275
整精米率 Head rice rate (%)	41.27	61.02	19.75	54.10	5.86	66.00	37.00	9.23	-0.389	-0.48

性状 Trait	QTL	标记区间 Marker interval	区间距离 Interval distance/cM	LOD值			贡献率PVE(%)			加性效应Add(%)
性状 Trait	QTL	标记区间 Marker interval	区间距离 Interval distance/cM	2019	2020	2021	2019	2020	2021	2019	2020	2021
BR	qBR-11	Marker1690523-Marker1651369	0.623	6.06			75.32			-1.21
BR	qBR-4	Marker2308870-Marker2433873	0.21		2.53			18.68			0.47
MR	qMR-5	Marker49932-Marker141199	0.415	10.62			16.21			2.24
	qMR-1	Marker405598-Marker617137	0.415		3.7			4.35			0.35
	qMR-2	Marker2776334-Marker2942331	0			2.6			0.75			-0.5
HR	qHR-5	Marker160033-Marker137238	0.419	6.95			10.76			2.7
	qHR-6-1	Marker3365801-Marker3142370	0.42		4.07			5.86			1.25
	qHR-6-2	Marker3340147-Marker3099891	0.208			3.41			5.42			-2.47
	qHR-8	Marker2591821-Marker2551026	0			3.32			3.66			2.03

性状 Trait	QTL	标记区间 Marker interval	区间距离 Interval distance/cM	LOD值			贡献率PVE(%)			加性效应Add(%)
性状 Trait	QTL	标记区间 Marker interval	区间距离 Interval distance/cM	2019	2020	2021	2019	2020	2021	2019	2020	2021
BR	qBR-11	Marker1690523-Marker1651369	0.623	6.06			75.32			-1.21
BR	qBR-4	Marker2308870-Marker2433873	0.21		2.53			18.68			0.47
MR	qMR-5	Marker49932-Marker141199	0.415	10.62			16.21			2.24
	qMR-1	Marker405598-Marker617137	0.415		3.7			4.35			0.35
	qMR-2	Marker2776334-Marker2942331	0			2.6			0.75			-0.5
HR	qHR-5	Marker160033-Marker137238	0.419	6.95			10.76			2.7
	qHR-6-1	Marker3365801-Marker3142370	0.42		4.07			5.86			1.25
	qHR-6-2	Marker3340147-Marker3099891	0.208			3.41			5.42			-2.47
	qHR-8	Marker2591821-Marker2551026	0			3.32			3.66			2.03