Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 2058-2064.DOI: 10.6048/j.issn.1001-4330.2022.08.028
• Prataculture·Animal Husbandry Veterinarian • Previous Articles Next Articles
ZHANG Tao1(), HU Jiangwei1, LIU Hong1, LI Dong2, SHA Wenjun2, WANG Xiaoyu2, CAI Lingang1, NIU Jiangong1(
)
Received:
2021-07-13
Online:
2022-08-20
Published:
2022-10-01
Correspondence author:
NIU Jiangong
Supported by:
张涛1(), 胡江伟1, 刘鸿1, 李冬2, 沙文军2, 王小渝2, 蔡林钢1, 牛建功1(
)
通讯作者:
牛建功
作者简介:
张涛(1992-), 男, 工程师, 硕士, 研究方向为水产动物增养殖与水环境监测,(E-mail) 872799786@qq.com
基金资助:
CLC Number:
ZHANG Tao, HU Jiangwei, LIU Hong, LI Dong, SHA Wenjun, WANG Xiaoyu, CAI Lingang, NIU Jiangong. Acute Toxicity of Three Aquatic Drugs to the Gymnodiptychus dybowskii[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 2058-2064.
张涛, 胡江伟, 刘鸿, 李冬, 沙文军, 王小渝, 蔡林钢, 牛建功. 分析3种水产药物对新疆裸重唇鱼幼鱼的急性毒性影响[J]. 新疆农业科学, 2022, 59(8): 2058-2064.
试验药物 Tested drug | 性状 Traits | 主要成分 Main ingredients | 生产厂家 Manufacturer |
---|---|---|---|
甲醛 Formaldehyde | 无色液体 | 40%(分析纯) | 无锡市亚泰联合化工有限公司 |
食盐 Salt | 白色晶体 | 99.1% NaCl | 久大(应城)盐矿有限责任公司 |
高聚碘 Highpolyiodine | 深褐色液体 | 碘(有效碘1.5%) | 宜兴市苏亚达渔业科技有限公司 |
Table 1 The specifications and manufacturers of the drugs used in the test
试验药物 Tested drug | 性状 Traits | 主要成分 Main ingredients | 生产厂家 Manufacturer |
---|---|---|---|
甲醛 Formaldehyde | 无色液体 | 40%(分析纯) | 无锡市亚泰联合化工有限公司 |
食盐 Salt | 白色晶体 | 99.1% NaCl | 久大(应城)盐矿有限责任公司 |
高聚碘 Highpolyiodine | 深褐色液体 | 碘(有效碘1.5%) | 宜兴市苏亚达渔业科技有限公司 |
药物名称 Drug names | 药物浓度Drug Concentration(mg/L) | |||||||
---|---|---|---|---|---|---|---|---|
对照组 Control group | Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | |
甲醛 Formaldehyde | 0 | 25.00 | 34.58 | 47.82 | 66.14 | 91.48 | 126.53 | 175.00 |
食盐 Salt | 0 | 11 000.00 | 11 583.57 | 12 198.10 | 12 845.23 | 13 526.70 | 14 244.31 | 15 000.00 |
高聚碘 Highpolyiodine | 0 | 20.00 | 24.02 | 28.84 | 34.64 | 41.60 | 49.96 | 60.00 |
Table 2 Test Concentration of three drugs
药物名称 Drug names | 药物浓度Drug Concentration(mg/L) | |||||||
---|---|---|---|---|---|---|---|---|
对照组 Control group | Ⅰ | Ⅱ | Ⅲ | Ⅳ | Ⅴ | Ⅵ | Ⅶ | |
甲醛 Formaldehyde | 0 | 25.00 | 34.58 | 47.82 | 66.14 | 91.48 | 126.53 | 175.00 |
食盐 Salt | 0 | 11 000.00 | 11 583.57 | 12 198.10 | 12 845.23 | 13 526.70 | 14 244.31 | 15 000.00 |
高聚碘 Highpolyiodine | 0 | 20.00 | 24.02 | 28.84 | 34.64 | 41.60 | 49.96 | 60.00 |
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
25.00 | 0 | 0 | 0 | 0 |
34.58 | 0 | 0 | 5 | 10 |
47.82 | 0 | 15 | 15 | 25 |
66.14 | 15 | 40 | 65 | 100 |
91.48 | 65 | 95 | 100 | - |
126.53 | 80 | 100 | - | - |
175.00 | 100 | - | - | - |
Table 3 Acute toxicity of formaldehyde to Gymnodiptychusdybowskiiseedling
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
25.00 | 0 | 0 | 0 | 0 |
34.58 | 0 | 0 | 5 | 10 |
47.82 | 0 | 15 | 15 | 25 |
66.14 | 15 | 40 | 65 | 100 |
91.48 | 65 | 95 | 100 | - |
126.53 | 80 | 100 | - | - |
175.00 | 100 | - | - | - |
时间 Time (h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 188.13x - 316.99 | 0.959 1 | 89.27 | 12.48 |
48 | y = 164.31x - 252.36 | 0.898 4 | 69.21 | |
72 | y = 184.61x - 273.07 | 0.886 0 | 56.23 | |
96 | y = 223.66x - 326.17 | 0.804 3 | 48.07 |
Table 4 Semi-lethal concentration(LC50) and safe concentration(SC) of formaldehyde to Gymnodiptychus dybowskii seedling
时间 Time (h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 188.13x - 316.99 | 0.959 1 | 89.27 | 12.48 |
48 | y = 164.31x - 252.36 | 0.898 4 | 69.21 | |
72 | y = 184.61x - 273.07 | 0.886 0 | 56.23 | |
96 | y = 223.66x - 326.17 | 0.804 3 | 48.07 |
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
11 000.00 | 0 | 0 | 0 | 0 |
11 583.57 | 0 | 0 | 0 | 5 |
12 198.10 | 0 | 5 | 10 | 20 |
12 845.23 | 15 | 20 | 30 | 45 |
13 526.70 | 45 | 90 | 100 | - |
14 244.31 | 80 | 100 | - | - |
15 000.00 | 100 | - | - | - |
Table 5 Acute toxicity of salt to Gymnodiptychus dybowskii seedling
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
11 000.00 | 0 | 0 | 0 | 0 |
11 583.57 | 0 | 0 | 0 | 5 |
12 198.10 | 0 | 5 | 10 | 20 |
12 845.23 | 15 | 20 | 30 | 45 |
13 526.70 | 45 | 90 | 100 | - |
14 244.31 | 80 | 100 | - | - |
15 000.00 | 100 | - | - | - |
时间 Time(h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 1 180.4x - 4 828.5 | 0.984 9 | 13 580.67 | 3 582.37 |
48 | y = 1 269.5x - 5 173.1 | 0.875 3 | 13 010.59 | |
72 | y = 1 425.4x -5 805.6 | 0.839 3 | 12 824.48 | |
96 | y = 1 069.1x - 4 334.5 | 0.863 6 | 12 621.56 |
Table 6 LC50 and SC of salt to Gymnodiptychus dybowskii seedling
时间 Time(h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 1 180.4x - 4 828.5 | 0.984 9 | 13 580.67 | 3 582.37 |
48 | y = 1 269.5x - 5 173.1 | 0.875 3 | 13 010.59 | |
72 | y = 1 425.4x -5 805.6 | 0.839 3 | 12 824.48 | |
96 | y = 1 069.1x - 4 334.5 | 0.863 6 | 12 621.56 |
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
20.00 | 0 | 0 | 0 | 0 |
24.02 | 0 | 0 | 5 | 10 |
28.84 | 0 | 10 | 20 | 20 |
34.64 | 5 | 15 | 30 | 45 |
41.60 | 20 | 55 | 80 | 100 |
49.96 | 70 | 100 | - | - |
60.00 | 100 | - | - | - |
Table 7 Acute toxicity of high polyiodine to Gymnodiptychus dybowskii seedling
药物浓度 Drug concentration (mg/L) | 死亡率 Mortality(%) | |||
---|---|---|---|---|
24 h | 48 h | 72 h | 96 h | |
0 | 0 | 0 | 0 | 0 |
20.00 | 0 | 0 | 0 | 0 |
24.02 | 0 | 0 | 5 | 10 |
28.84 | 0 | 10 | 20 | 20 |
34.64 | 5 | 15 | 30 | 45 |
41.60 | 20 | 55 | 80 | 100 |
49.96 | 70 | 100 | - | - |
60.00 | 100 | - | - | - |
时间 Time (h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 333.17x -500.44 | 0.909 6 | 44.89 | 8.47 |
48 | y = 308.11x - 438.36 | 0.873 8 | 38.46 | |
72 | y = 264.10x -356.93 | 0.905 8 | 34.74 | |
96 | y = 295.56x - 396.54 | 0.863 0 | 32.42 |
Table 8 LC50 and SC of high polyiodine to Gymnodiptychus dybowskii seedling
时间 Time (h) | 回归方程 Regression equation | 相关系数 Relativecoefficient(r2) | 半致死浓度 LC50 (mg/L) | 安全浓度 SC (mg/L) |
---|---|---|---|---|
24 | y = 333.17x -500.44 | 0.909 6 | 44.89 | 8.47 |
48 | y = 308.11x - 438.36 | 0.873 8 | 38.46 | |
72 | y = 264.10x -356.93 | 0.905 8 | 34.74 | |
96 | y = 295.56x - 396.54 | 0.863 0 | 32.42 |
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