Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (7): 1766-1772.DOI: 10.6048/j.issn.1001-4330.2023.07.024
• Microbes·Animal Husbandry Veterinarian • Previous Articles Next Articles
LI Yixin1,2(), CHEN Yong3, LIU Xiaolu2, Ainijiang Ersiman1, XU Lijuan1,4, LIU Qianqian5, BAO Xiaowei2(
), SONG Suqin1(
)
Received:
2022-10-07
Online:
2023-07-20
Published:
2023-07-11
Correspondence author:
BAO Xiaowei(1969-),female,Zhejian somgyang county, associate professor, major in food nutrition and safety,(E-mail)408531623@qq.com;Supported by:
李怡歆1,2(), 陈勇3, 刘晓禄2, 艾尼江·尔斯满1, 徐李娟1,4, 刘倩倩5, 包晓玮2(
), 宋素琴1(
)
通讯作者:
包晓玮(1969-),女,浙江松阳人,副教授,博士,研究方向为食品营养与安全,(E-mail)408531623@qq.com;作者简介:
李怡歆(1997-),女,新疆伊犁人,硕士研究生,研究方向为放线菌活性,(E-mail)2463514716@qq.com
基金资助:
CLC Number:
LI Yixin, CHEN Yong, LIU Xiaolu, Ainijiang Ersiman, XU Lijuan, LIU Qianqian, BAO Xiaowei, SONG Suqin. Isolation, identification and activity of halophilic bacteria from Dabancheng Salt Lake, Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1766-1772.
李怡歆, 陈勇, 刘晓禄, 艾尼江·尔斯满, 徐李娟, 刘倩倩, 包晓玮, 宋素琴. 新疆达坂城盐湖嗜盐细菌分离鉴定及活性分析[J]. 新疆农业科学, 2023, 60(7): 1766-1772.
培养基 Culture medium | 嗜盐细菌属 Halophilic bacteria | 嗜盐细菌种 Halophilic bacterial species |
---|---|---|
M-2(海藻糖-脯氨酸) | 类芽孢杆菌属(Paenibacillus) | Paenibacillus peoriae |
MM琼脂 | 代谢杆菌(Metabacillus) | Metabacillus sediminilitoris |
M-7(甘油-天门冬酰胺) | 类诺卡氏菌属(Nocardioides) 动性球菌属(Planococcus) 冢村氏菌属(Tsukamurella) 短杆菌属(Brevibacterium) 假黄色单胞菌属(Pseudoxanthomonas) 链霉菌属(Streptomyces) 红球菌属(Rhodococcus) 小单胞菌属(Micromonospora) 芽孢杆菌属(Bacillus) 细杆菌属(Microbacterium paraoxydans) 微球菌属(Micrococcus) 拟诺卡氏菌属(Nocardiopsis) | Nocardioides furvisabuli、Nocardioides LTZG-s、Tsukamurella conjunctivitidis、Brevibacterium epidermidis、Pseudoxanthomonas japonensis、Streptomyces longispororuber、Rhodococcus qingshengii、Micromonospora oryzae、Bacillus paramycoides、Microbacterium paraoxydans、Micrococcus antarcticus、Tsukamurella_sinensis、Nocardiopsis dassonvillei |
M-1(TWYE) | 链霉菌属(Streptomyces) | Streptomyces pratensis |
Tab.1 Species of halophilic bacteria isolated from different media
培养基 Culture medium | 嗜盐细菌属 Halophilic bacteria | 嗜盐细菌种 Halophilic bacterial species |
---|---|---|
M-2(海藻糖-脯氨酸) | 类芽孢杆菌属(Paenibacillus) | Paenibacillus peoriae |
MM琼脂 | 代谢杆菌(Metabacillus) | Metabacillus sediminilitoris |
M-7(甘油-天门冬酰胺) | 类诺卡氏菌属(Nocardioides) 动性球菌属(Planococcus) 冢村氏菌属(Tsukamurella) 短杆菌属(Brevibacterium) 假黄色单胞菌属(Pseudoxanthomonas) 链霉菌属(Streptomyces) 红球菌属(Rhodococcus) 小单胞菌属(Micromonospora) 芽孢杆菌属(Bacillus) 细杆菌属(Microbacterium paraoxydans) 微球菌属(Micrococcus) 拟诺卡氏菌属(Nocardiopsis) | Nocardioides furvisabuli、Nocardioides LTZG-s、Tsukamurella conjunctivitidis、Brevibacterium epidermidis、Pseudoxanthomonas japonensis、Streptomyces longispororuber、Rhodococcus qingshengii、Micromonospora oryzae、Bacillus paramycoides、Microbacterium paraoxydans、Micrococcus antarcticus、Tsukamurella_sinensis、Nocardiopsis dassonvillei |
M-1(TWYE) | 链霉菌属(Streptomyces) | Streptomyces pratensis |
Fig.1 Part of halophilic bacteria in Dabancheng Salt Lake, Xinjiang Note:a:Strain DBC23; b:Strain DBC30; c:Strain DBC31; d:Strain DBC5; e:Strain DBC9; f:Strain DBC10
序列号 NO. | 菌株编号 NO. | 最近相似菌株名称 The name of most similarity bacteria | 相似度 Similarity | 收藏号 Accession NO. |
---|---|---|---|---|
1 | DBC1 | Bacillus mobilis | 99.51% | MACF01000036 |
2 | DBC2 | Pseudoxanthomonas japonensis | 99.14% | AB008507 |
3 | DBC3 | Micromonospora oryzae | 99.35% | AB981052 |
4 | DBC4 | Rhodococcus qingshengii | 99.64% | LRRJ01000016 |
5 | DBC5 | Streptomyces longispororuber | 99.71% | AB184440 |
6 | DBC7 | Brevibacterium epidermidis | 99.60% | BCSJ01000023 |
7 | DBC9 | Tsukamurella conjunctivitidis | 99.71% | MK583684 |
8 | DBC25 | LTZG_s | 99.10% | LTZG01000007 |
9 | DBC23 | Nocardioides alpinus | 97.43% | GU784866 |
10 | DBC10 | Streptomyces pratensis | 99.85% | JQ806215 |
11 | DBC12 | Metabacillus sediminilitoris | 98.20% | MN067806 |
12 | DBC13 | MRTF_s | 98.90% | MRTF01000019 |
13 | DBC18 | Bacillus paramycoides | 95.93% | MAOI01000012 |
14 | DBC22 | Microbacterium paraoxydans | 99.22% | BCRH01000180 |
15 | DBC24 | Micrococcus antarcticus | 98.93% | AJ005932 |
16 | DBC26 | Tsukamurella sinensis | 99.64% | KP888571 |
17 | DBC30 | Nocardiopsis dassonvillei | 100% | ABUI01000017 |
18 | DBC31 | Nocardiopsis crassaminis | 99.82% | LR606207 |
Tab.2 Sequence similarity of 16S rRNA between isolated strains and known strains
序列号 NO. | 菌株编号 NO. | 最近相似菌株名称 The name of most similarity bacteria | 相似度 Similarity | 收藏号 Accession NO. |
---|---|---|---|---|
1 | DBC1 | Bacillus mobilis | 99.51% | MACF01000036 |
2 | DBC2 | Pseudoxanthomonas japonensis | 99.14% | AB008507 |
3 | DBC3 | Micromonospora oryzae | 99.35% | AB981052 |
4 | DBC4 | Rhodococcus qingshengii | 99.64% | LRRJ01000016 |
5 | DBC5 | Streptomyces longispororuber | 99.71% | AB184440 |
6 | DBC7 | Brevibacterium epidermidis | 99.60% | BCSJ01000023 |
7 | DBC9 | Tsukamurella conjunctivitidis | 99.71% | MK583684 |
8 | DBC25 | LTZG_s | 99.10% | LTZG01000007 |
9 | DBC23 | Nocardioides alpinus | 97.43% | GU784866 |
10 | DBC10 | Streptomyces pratensis | 99.85% | JQ806215 |
11 | DBC12 | Metabacillus sediminilitoris | 98.20% | MN067806 |
12 | DBC13 | MRTF_s | 98.90% | MRTF01000019 |
13 | DBC18 | Bacillus paramycoides | 95.93% | MAOI01000012 |
14 | DBC22 | Microbacterium paraoxydans | 99.22% | BCRH01000180 |
15 | DBC24 | Micrococcus antarcticus | 98.93% | AJ005932 |
16 | DBC26 | Tsukamurella sinensis | 99.64% | KP888571 |
17 | DBC30 | Nocardiopsis dassonvillei | 100% | ABUI01000017 |
18 | DBC31 | Nocardiopsis crassaminis | 99.82% | LR606207 |
植物病原菌Plant pathogen | |||||
---|---|---|---|---|---|
嗜盐放线菌 Halophilic actinomycetes | 南瓜枯萎病菌 F.oxysporum | 水稻恶苗病菌 F.moliniforme | 棉花枯萎病菌 F.oxysporum | 核桃腐烂病菌 Cytcospora sp. | 苹果树腐烂病菌 Valsa mali var.mali |
DBC5 | — | + | — | — | — |
DBC23 | — | — | — | — | — |
DBC10 | — | — | — | — | — |
DBC26 | — | — | + | — | — |
DBC30 | — | — | — | — | — |
DBC31 | — | — | + | — | — |
Tab.3 Antibacterial activity of halophilic actinomycetes in Dabancheng Salt Lake
植物病原菌Plant pathogen | |||||
---|---|---|---|---|---|
嗜盐放线菌 Halophilic actinomycetes | 南瓜枯萎病菌 F.oxysporum | 水稻恶苗病菌 F.moliniforme | 棉花枯萎病菌 F.oxysporum | 核桃腐烂病菌 Cytcospora sp. | 苹果树腐烂病菌 Valsa mali var.mali |
DBC5 | — | + | — | — | — |
DBC23 | — | — | — | — | — |
DBC10 | — | — | — | — | — |
DBC26 | — | — | + | — | — |
DBC30 | — | — | — | — | — |
DBC31 | — | — | + | — | — |
菌株编号 Strain No. | 抑制率 Inhibition rate |
---|---|
DBC5 | 96.4% |
DBC10 | 87.8% |
DBC9 | 91.8% |
DBC3 | NA |
DBC26 | 83.2% |
DBC24 | 16.7% |
Tab.4 Inhibition of crude extract of salt lake actinomycetes fermentation broth on HepG2 cells
菌株编号 Strain No. | 抑制率 Inhibition rate |
---|---|
DBC5 | 96.4% |
DBC10 | 87.8% |
DBC9 | 91.8% |
DBC3 | NA |
DBC26 | 83.2% |
DBC24 | 16.7% |
[1] | 高波, 吉兰泰盐湖土壤中嗜盐碱放线菌的分离鉴定及四氢嘧啶工程菌株的构建[D] 杨凌: 西北农林科技大学, 2016. |
GAO Bo. Isolation and identification of saline-alkali actinomycetes from soil of Jilantai Salt Lake and construction of tetrahydropyrimidine engineering strain[D]. Yangling: Northwest A&F University, 2016. | |
[2] |
Hacěne H, Bhatnagar J C, Baratti B. Biodiversity of prokaryotic microflora in El Golea Salt lake, Algerian Sahara[J]. Journal of Arid Environments, 2004, 58(3):273-284.
DOI URL |
[3] |
Shantikumar L. Author's personal copy Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D[J]. Journal de Mycologie Médicale, 2009, 19(4):225-233.
DOI URL |
[4] | 吴海平, 王真辉, 杨礼富. 新疆达坂盐湖沉积土壤嗜盐细菌的定向富集与多样性分析[J]. 微生物学通报, 2010, 37(7):956-961. |
WU Haiping, WANG Zhenhui, YANG Lifu. Analysis on the diversity and enrichment of halophilic bacteria in sediments of daban salt lake, Xinjiang[J]. Microbiology Bulletin, 2010, 37(7):956-961. | |
[5] | 刘会强, 赵春梅, 黄瑞虎. 新疆达坂城盐湖中度嗜盐菌的分类研究[J]. 新疆师范大学学报(自然科学版), 2007,(3):146-149. |
LIU Huiqiang, ZHAO Chunmei, HUANG Ruihu. Classification of moderate halophilic bacteria in Dabancheng Salt Lake, Xinjiang[J]. Journal of Xinjiang Normal University (Natural Science Ed.), 2007,(3):146-149. | |
[6] | 张立丰. 新疆达坂城盐湖嗜盐古菌16S rDNA序列分析和细菌视紫红质基因序列的研究[D]. 乌鲁木齐: 新疆师范大学, 2006. |
ZHANG Lifeng. 16S rDNA sequence analysis and bacterial rhodopsin gene sequence of halophilic archaea from Dabancheng Salt Lake, Xinjiang[D]. Urumqi: Xinjiang Normal University, 2006. | |
[7] | 关统伟. 新疆罗布泊盐湖放线菌多样性及多相分类[D]. 成都: 四川农业大学, 2012. |
GUAN Tongwei. Diversity and multiphase classification of actinomycetes in Lop Nur Salt Lake, Xinjiang[D]. Chengdu: Sichuan Agricultural University, 2012. | |
[8] | 宋阳, 王秋菊, 杨旭, 等. 新疆盐碱土壤耐盐碱放线菌筛选初步鉴定[J]. 生物化工, 2020, 6(3):80-84. |
SONG Yang, WANG Qiuju, YANG Xu, et al. Preliminary screening and identification of saline-alkali tolerant actinomycetes in xinjiang saline-alkali soil[J]. Biochemical Engineering, 2020, 6(3):80-84. | |
[9] | 李文均, 焦建宇. 放线菌分类地位的变迁及其系统学研究最新进展[J]. 微生物学杂志, 2020, 40(1):1-14. |
LI Wenjun, JIAO Jainyu. Changes in taxonomic status of actinomycetes and recent advances in systematic research[J]. Journal of Microbiology, 2020, 40(1):1-14. | |
[10] |
Skehan P, Storeng R, Scudiero D, et al. New colorimetric cytotoxicity assay for anticancer-drug screening[J]. Journal of the National Cancer Institute, 1990, 82(13):1107-1112.
DOI PMID |
[11] | 任海柯, 死海极端高盐环境放线菌资源初步研究[D]. 杨凌: 西北农林科技大学, 2012. |
REN Haike. Preliminary study on actinomycetes resources in extreme saline environment of the Dead Sea[D]. Yangling: Northwest A&F University, 2012. | |
[12] | 李二阳, 马雪莉, 吕杰, 等. 新疆天山北坡不同盐湖微生物菌群结构及其影响因子[J]. 生态学报, 2021, 41(18):7212-7225. |
LI Eryang, MA Xueli, LV Jie, et al. Microflora structure and its influencing factors in different salt lakes on the Northern Slope of Tianshan Mountains, Xinjiang[J]. Acta Ecologica Sinica, 2021, 41(18):7212-7225. | |
[13] | 唐蜀昆, 李文均, 张永光, 等. 嗜盐放线菌生物学特性初步研究[J]. 微生物学通报, 2003,(4):15-19. |
TANG Shukun, LI Wenjun, ZHANG Yonghua, et al. Preliminary study on biological characteristics of halophilic actinomycetes[J]. Microbiology Bulletin, 2003,(4):15-19. | |
[14] |
Babich H, Stotzky G. Temperature, pH, salinity, hardness, and particulates mediate nickel toxicity to eubacteria, an actinomycete, and yeasts in lake, simulated estuarine, and sea waters[J]. Aquatic Toxicology, 1983, 3(3):195-208.
DOI URL |
[1] | ZENG Wanying, GENG Hongwei, CHENG Yukun, LI Sizhong, QIAN Songting, GAO Weishi, ZHANG Liming. Comprehensive evaluation of drought resistance during the rapid growth stage of sugar beet cultivars [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2140-2151. |
[2] | XU Maomao, GAO Jie, LI Junming, LI Xin, LIU Lei, PAN Feng. Population diversity analysis of 20 commercial tomato cultivars [J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2191-2196. |
[3] | MIAO Yu, CHEN Cuixia, MA Yanming, XING Guofang, DONG Yusheng, CHEN Zhijun, WANG Xian, XIANG Li. Genetic diversity analysis of phenotypic traits of 276 Central Asian barley germplasm resources [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1888-1895. |
[4] | WANG Qingpeng, YAN Chengcai, WANG Zhe, GOU Changqing, WANG Lan, FENG Hongzu, HAO Haiting. Anther bacterial diversity of Korla Fragrant Pear before and after flowering [J]. Xinjiang Agricultural Sciences, 2024, 61(8): 1976-1982. |
[5] | LIU Huifang, HAN Hongwei, ZHUANG Hongmei, WANG Qiang, GAO Axiang, WANG Hao. Effects of increased application of microbial fertilizer on soil microbial diversity of cucumber in facilities [J]. Xinjiang Agricultural Sciences, 2024, 61(7): 1727-1737. |
[6] | YANG Lu, WANG Na, FAN Shaoli, CHENG Ping, LI Hong, WANG Yangdong. Analysis of phenotypic trait variation characteristics of Morus nigra L.germplasm resources [J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1172-1181. |
[7] | GENG Meiju, WANG Xinhui, LIU Xiaoying, LYU Pei. Effects of sealing on fungal communities in desert grasslands [J]. Xinjiang Agricultural Sciences, 2024, 61(5): 1250-1258. |
[8] | YAO Yuxiang, WANG Guoqiang, WANG Ke, YI Sha, YANG Xinya, LUO Xioaxia. Analysis of the bacterial communities structure and diversty of the Tarim River in different habitats [J]. Xinjiang Agricultural Sciences, 2024, 61(3): 708-718. |
[9] | WANG Fan, LI Yushan, WANG Wei, DENG Chaohong, ZHAO Lianjia, MA Yue, XIAO Jing, ZHUANG Hongmei, Xu Hongjun. Genetic diversity analysis of major nutritional growth traits in 74 turnip germplasm resources [J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2601-2613. |
[10] | LI Chao, YANG Ying, ZHENG Heyun, YANG Jianli, CHEN Wei, YANG Mi, SUN Yuping. Genetic diversity and population structure analysis of melon germplasm resources in Xinjiang based on SSR fluorescence markers [J]. Xinjiang Agricultural Sciences, 2024, 61(11): 2614-2625. |
[11] | HUANG Xinglei, WANG Weiran, WANG Meng, ZHU Jiahui, LIN Feng, QIN Guoli, YANG Jing, Alifu Aierxi, WU Quanzhong, KONG Jie. Diversity evaluation of machine-picked agronomic traits in Gossypium barbadense L. germplasm resources [J]. Xinjiang Agricultural Sciences, 2024, 61(1): 1-8. |
[12] | XI Ouyan, WANG Chenri, Gulinuer Tulaxi, HU Hongying. Characteristics of pollinating insect types and floral visiting behavior in wild fruit forests of the Ili Valley [J]. Xinjiang Agricultural Sciences, 2024, 61(1): 190-198. |
[13] | Wang Tianling, Hou Xianfei, Shi Junjie, Sun Quanxi, Jia Donghai, Gu Yuanguo, Shan Shihua, Miao Haocui, Li Qiang. Genetic diversity analysis of 67 creeping peanut germplasm resources [J]. Xinjiang Agricultural Sciences, 2024, 61(1): 42-54. |
[14] | WANG Dandan, LI Yan, ZHANG Qingyin, Li Shidong, PANG Yongchao, MA Kunzhi, MA Long, NIU Ruisheng, ZHONG Zengming, QI Lianfen, SHI Jianhua. Effects of different microbial treatments on tomato soil microbial diversity [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2248-2257. |
[15] | ZHOU Shijie, DONG Yiqiang, Asitaiken Julihaiti, NIE Tingting, JIANG Anjing, AN Shazhou. Quantitative characteristics and diversity of sagebrush desert plant communities on the northern slope of Tianshan Mountains [J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2298-2305. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||