Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (7): 1766-1772.DOI: 10.6048/j.issn.1001-4330.2023.07.024
• Microbes·Animal Husbandry Veterinarian • Previous Articles Next Articles
LI Yixin1,2(), CHEN Yong3, LIU Xiaolu2, Ainijiang Ersiman1, XU Lijuan1,4, LIU Qianqian5, BAO Xiaowei2(), SONG Suqin1()
Received:
2022-10-07
Online:
2023-07-20
Published:
2023-07-11
Correspondence author:
BAO Xiaowei(1969-),female,Zhejian somgyang county, associate professor, major in food nutrition and safety,(E-mail)408531623@qq.com;Supported by:
李怡歆1,2(), 陈勇3, 刘晓禄2, 艾尼江·尔斯满1, 徐李娟1,4, 刘倩倩5, 包晓玮2(), 宋素琴1()
通讯作者:
包晓玮(1969-),女,浙江松阳人,副教授,博士,研究方向为食品营养与安全,(E-mail)408531623@qq.com;作者简介:
李怡歆(1997-),女,新疆伊犁人,硕士研究生,研究方向为放线菌活性,(E-mail)2463514716@qq.com
基金资助:
CLC Number:
LI Yixin, CHEN Yong, LIU Xiaolu, Ainijiang Ersiman, XU Lijuan, LIU Qianqian, BAO Xiaowei, SONG Suqin. Isolation, identification and activity of halophilic bacteria from Dabancheng Salt Lake, Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1766-1772.
李怡歆, 陈勇, 刘晓禄, 艾尼江·尔斯满, 徐李娟, 刘倩倩, 包晓玮, 宋素琴. 新疆达坂城盐湖嗜盐细菌分离鉴定及活性分析[J]. 新疆农业科学, 2023, 60(7): 1766-1772.
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培养基 Culture medium | 嗜盐细菌属 Halophilic bacteria | 嗜盐细菌种 Halophilic bacterial species |
---|---|---|
M-2(海藻糖-脯氨酸) | 类芽孢杆菌属(Paenibacillus) | Paenibacillus peoriae |
MM琼脂 | 代谢杆菌(Metabacillus) | Metabacillus sediminilitoris |
M-7(甘油-天门冬酰胺) | 类诺卡氏菌属(Nocardioides) 动性球菌属(Planococcus) 冢村氏菌属(Tsukamurella) 短杆菌属(Brevibacterium) 假黄色单胞菌属(Pseudoxanthomonas) 链霉菌属(Streptomyces) 红球菌属(Rhodococcus) 小单胞菌属(Micromonospora) 芽孢杆菌属(Bacillus) 细杆菌属(Microbacterium paraoxydans) 微球菌属(Micrococcus) 拟诺卡氏菌属(Nocardiopsis) | Nocardioides furvisabuli、Nocardioides LTZG-s、Tsukamurella conjunctivitidis、Brevibacterium epidermidis、Pseudoxanthomonas japonensis、Streptomyces longispororuber、Rhodococcus qingshengii、Micromonospora oryzae、Bacillus paramycoides、Microbacterium paraoxydans、Micrococcus antarcticus、Tsukamurella_sinensis、Nocardiopsis dassonvillei |
M-1(TWYE) | 链霉菌属(Streptomyces) | Streptomyces pratensis |
Tab.1 Species of halophilic bacteria isolated from different media
培养基 Culture medium | 嗜盐细菌属 Halophilic bacteria | 嗜盐细菌种 Halophilic bacterial species |
---|---|---|
M-2(海藻糖-脯氨酸) | 类芽孢杆菌属(Paenibacillus) | Paenibacillus peoriae |
MM琼脂 | 代谢杆菌(Metabacillus) | Metabacillus sediminilitoris |
M-7(甘油-天门冬酰胺) | 类诺卡氏菌属(Nocardioides) 动性球菌属(Planococcus) 冢村氏菌属(Tsukamurella) 短杆菌属(Brevibacterium) 假黄色单胞菌属(Pseudoxanthomonas) 链霉菌属(Streptomyces) 红球菌属(Rhodococcus) 小单胞菌属(Micromonospora) 芽孢杆菌属(Bacillus) 细杆菌属(Microbacterium paraoxydans) 微球菌属(Micrococcus) 拟诺卡氏菌属(Nocardiopsis) | Nocardioides furvisabuli、Nocardioides LTZG-s、Tsukamurella conjunctivitidis、Brevibacterium epidermidis、Pseudoxanthomonas japonensis、Streptomyces longispororuber、Rhodococcus qingshengii、Micromonospora oryzae、Bacillus paramycoides、Microbacterium paraoxydans、Micrococcus antarcticus、Tsukamurella_sinensis、Nocardiopsis dassonvillei |
M-1(TWYE) | 链霉菌属(Streptomyces) | Streptomyces pratensis |
Fig.1 Part of halophilic bacteria in Dabancheng Salt Lake, Xinjiang Note:a:Strain DBC23; b:Strain DBC30; c:Strain DBC31; d:Strain DBC5; e:Strain DBC9; f:Strain DBC10
序列号 NO. | 菌株编号 NO. | 最近相似菌株名称 The name of most similarity bacteria | 相似度 Similarity | 收藏号 Accession NO. |
---|---|---|---|---|
1 | DBC1 | Bacillus mobilis | 99.51% | MACF01000036 |
2 | DBC2 | Pseudoxanthomonas japonensis | 99.14% | AB008507 |
3 | DBC3 | Micromonospora oryzae | 99.35% | AB981052 |
4 | DBC4 | Rhodococcus qingshengii | 99.64% | LRRJ01000016 |
5 | DBC5 | Streptomyces longispororuber | 99.71% | AB184440 |
6 | DBC7 | Brevibacterium epidermidis | 99.60% | BCSJ01000023 |
7 | DBC9 | Tsukamurella conjunctivitidis | 99.71% | MK583684 |
8 | DBC25 | LTZG_s | 99.10% | LTZG01000007 |
9 | DBC23 | Nocardioides alpinus | 97.43% | GU784866 |
10 | DBC10 | Streptomyces pratensis | 99.85% | JQ806215 |
11 | DBC12 | Metabacillus sediminilitoris | 98.20% | MN067806 |
12 | DBC13 | MRTF_s | 98.90% | MRTF01000019 |
13 | DBC18 | Bacillus paramycoides | 95.93% | MAOI01000012 |
14 | DBC22 | Microbacterium paraoxydans | 99.22% | BCRH01000180 |
15 | DBC24 | Micrococcus antarcticus | 98.93% | AJ005932 |
16 | DBC26 | Tsukamurella sinensis | 99.64% | KP888571 |
17 | DBC30 | Nocardiopsis dassonvillei | 100% | ABUI01000017 |
18 | DBC31 | Nocardiopsis crassaminis | 99.82% | LR606207 |
Tab.2 Sequence similarity of 16S rRNA between isolated strains and known strains
序列号 NO. | 菌株编号 NO. | 最近相似菌株名称 The name of most similarity bacteria | 相似度 Similarity | 收藏号 Accession NO. |
---|---|---|---|---|
1 | DBC1 | Bacillus mobilis | 99.51% | MACF01000036 |
2 | DBC2 | Pseudoxanthomonas japonensis | 99.14% | AB008507 |
3 | DBC3 | Micromonospora oryzae | 99.35% | AB981052 |
4 | DBC4 | Rhodococcus qingshengii | 99.64% | LRRJ01000016 |
5 | DBC5 | Streptomyces longispororuber | 99.71% | AB184440 |
6 | DBC7 | Brevibacterium epidermidis | 99.60% | BCSJ01000023 |
7 | DBC9 | Tsukamurella conjunctivitidis | 99.71% | MK583684 |
8 | DBC25 | LTZG_s | 99.10% | LTZG01000007 |
9 | DBC23 | Nocardioides alpinus | 97.43% | GU784866 |
10 | DBC10 | Streptomyces pratensis | 99.85% | JQ806215 |
11 | DBC12 | Metabacillus sediminilitoris | 98.20% | MN067806 |
12 | DBC13 | MRTF_s | 98.90% | MRTF01000019 |
13 | DBC18 | Bacillus paramycoides | 95.93% | MAOI01000012 |
14 | DBC22 | Microbacterium paraoxydans | 99.22% | BCRH01000180 |
15 | DBC24 | Micrococcus antarcticus | 98.93% | AJ005932 |
16 | DBC26 | Tsukamurella sinensis | 99.64% | KP888571 |
17 | DBC30 | Nocardiopsis dassonvillei | 100% | ABUI01000017 |
18 | DBC31 | Nocardiopsis crassaminis | 99.82% | LR606207 |
植物病原菌Plant pathogen | |||||
---|---|---|---|---|---|
嗜盐放线菌 Halophilic actinomycetes | 南瓜枯萎病菌 F.oxysporum | 水稻恶苗病菌 F.moliniforme | 棉花枯萎病菌 F.oxysporum | 核桃腐烂病菌 Cytcospora sp. | 苹果树腐烂病菌 Valsa mali var.mali |
DBC5 | — | + | — | — | — |
DBC23 | — | — | — | — | — |
DBC10 | — | — | — | — | — |
DBC26 | — | — | + | — | — |
DBC30 | — | — | — | — | — |
DBC31 | — | — | + | — | — |
Tab.3 Antibacterial activity of halophilic actinomycetes in Dabancheng Salt Lake
植物病原菌Plant pathogen | |||||
---|---|---|---|---|---|
嗜盐放线菌 Halophilic actinomycetes | 南瓜枯萎病菌 F.oxysporum | 水稻恶苗病菌 F.moliniforme | 棉花枯萎病菌 F.oxysporum | 核桃腐烂病菌 Cytcospora sp. | 苹果树腐烂病菌 Valsa mali var.mali |
DBC5 | — | + | — | — | — |
DBC23 | — | — | — | — | — |
DBC10 | — | — | — | — | — |
DBC26 | — | — | + | — | — |
DBC30 | — | — | — | — | — |
DBC31 | — | — | + | — | — |
菌株编号 Strain No. | 抑制率 Inhibition rate |
---|---|
DBC5 | 96.4% |
DBC10 | 87.8% |
DBC9 | 91.8% |
DBC3 | NA |
DBC26 | 83.2% |
DBC24 | 16.7% |
Tab.4 Inhibition of crude extract of salt lake actinomycetes fermentation broth on HepG2 cells
菌株编号 Strain No. | 抑制率 Inhibition rate |
---|---|
DBC5 | 96.4% |
DBC10 | 87.8% |
DBC9 | 91.8% |
DBC3 | NA |
DBC26 | 83.2% |
DBC24 | 16.7% |
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