Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (7): 1606-1613.DOI: 10.6048/j.issn.1001-4330.2023.07.006
• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Soil Fertilizer • Previous Articles Next Articles
CHEN Zhanhui1(), SUN Qiang1, REN Jiaojiao1(), HUANG Bowen1, XU Jiabo1, YANG Jie2, WU Penghao1()
Received:
2022-10-30
Online:
2023-07-20
Published:
2023-07-11
Correspondence author:
WU Penghao(1984-),male,Hutubi, Xinjiang,professor,doctoral supervisor,mainly engaged in maize genetics and breeding,(E-mail)craie788@126.com;Supported by:
陈占辉1(), 孙强1, 任姣姣1(), 黄博文1, 许加波1, 杨杰2, 吴鹏昊1()
通讯作者:
吴鹏昊(1984-),男,新疆呼图壁人,教授,博士,博士生导师,研究方向为玉米遗传育种,(E-mail)craie788@126.com;作者简介:
陈占辉(1998-),男,青海海东人,硕士研究生,研究方向为玉米遗传育种,(E-mail)1291607673@qq.com
基金资助:
CLC Number:
CHEN Zhanhui, SUN Qiang, REN Jiaojiao, HUANG Bowen, XU Jiabo, YANG Jie, WU Penghao. QTL mapping and genomic selection of maize leaf width[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1606-1613.
陈占辉, 孙强, 任姣姣, 黄博文, 许加波, 杨杰, 吴鹏昊. 玉米叶宽的QTL定位及全基因组选择分析[J]. 新疆农业科学, 2023, 60(7): 1606-1613.
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亲本Parents | 环境 Environ- ment | 均值 Mean | 最小值 Max. | 最大值 Min. | 标准差 S.D. | 变幅 A.O.F | 变异 系数 CV. | 峰度 Kurtosis | 偏度 Skewness | |
---|---|---|---|---|---|---|---|---|---|---|
郑58 | B73 | |||||||||
8.02 | 9.04 | 海南 | 8.68 | 8.55 | 8.82 | 0.06 | 0.27 | 0.01 | 0.21 | -0.44 |
九圣禾 | 8.04 | 7.23 | 8.99 | 0.31 | 1.76 | 0.04 | 0.09 | -0.22 | ||
三坪 | 7.24 | 6.96 | 7.63 | 0.13 | 0.67 | 0.02 | 0.07 | -0.02 | ||
联合 | 8.00 | 7.78 | 8.19 | 0.07 | 0.41 | 0.01 | -0.17 | -0.11 |
Tab.1 Descriptive statistics for the leaf width of the two parent lines and F2∶3 families(cm)
亲本Parents | 环境 Environ- ment | 均值 Mean | 最小值 Max. | 最大值 Min. | 标准差 S.D. | 变幅 A.O.F | 变异 系数 CV. | 峰度 Kurtosis | 偏度 Skewness | |
---|---|---|---|---|---|---|---|---|---|---|
郑58 | B73 | |||||||||
8.02 | 9.04 | 海南 | 8.68 | 8.55 | 8.82 | 0.06 | 0.27 | 0.01 | 0.21 | -0.44 |
九圣禾 | 8.04 | 7.23 | 8.99 | 0.31 | 1.76 | 0.04 | 0.09 | -0.22 | ||
三坪 | 7.24 | 6.96 | 7.63 | 0.13 | 0.67 | 0.02 | 0.07 | -0.02 | ||
联合 | 8.00 | 7.78 | 8.19 | 0.07 | 0.41 | 0.01 | -0.17 | -0.11 |
变异来源 Source | 自由度 Degree of freedom | 平方和 Sum of square | 均方 Mean of square | F | P | 遗传力 H2 |
---|---|---|---|---|---|---|
环境Environment | 2 | 420.49 | 210.24 | 726.28 | <0.01** | 0.39 |
基因型Genotype | 199 | 109.78 | 0.55 | 1.91 | <0.01** | |
基因型环境互作GXE | 398 | 166.11 | 0.42 | 1.44 | <0.01** | |
误差Error | 597 | 172.82 | 0.29 | |||
总变异Variation | 1 196 | 869.20 |
Tab.2 ANOVA and broad-sense heritability analysis for the leaf width in F2∶3 families
变异来源 Source | 自由度 Degree of freedom | 平方和 Sum of square | 均方 Mean of square | F | P | 遗传力 H2 |
---|---|---|---|---|---|---|
环境Environment | 2 | 420.49 | 210.24 | 726.28 | <0.01** | 0.39 |
基因型Genotype | 199 | 109.78 | 0.55 | 1.91 | <0.01** | |
基因型环境互作GXE | 398 | 166.11 | 0.42 | 1.44 | <0.01** | |
误差Error | 597 | 172.82 | 0.29 | |||
总变异Variation | 1 196 | 869.20 |
QTL | 环境 Environ ment | 染色体 /Bin Chromo- some /Bin | 遗传位置 Genetic position (cM) | 物理距离 /Mba Physical position /Mba | 左翼标记 Left marker | 右翼标记 Right marker | LOD值 | 表型 贡献率 PVE (%) | 加性 效应 Add | 显性 效应 Dom | 基因 效应b Gene action |
---|---|---|---|---|---|---|---|---|---|---|---|
qew1-1 | 海南 | 1/1.06 | 74 | 196.18~197.14 | RM1_149 | RM1_150 | 2.73 | 5.04 | 0.1 | -0.06 | PD |
qew3-1 | 海南 | 3/3.07 | 52 | 198.91~198.92 | RM3_117 | RM3_118 | 2.93 | 5.51 | -0.1 | -0.01 | A |
qew5-1 | 海南 | 5/5.01 | 17 | 8.86~8.90 | RM5_27 | RM5_26 | 8.06 | 16.17 | 0.2 | 0.01 | A |
qew8-1 | 海南 | 8/8.02 | 13 | 13.54~13.64 | RM8_22 | RM8_23 | 2.52 | 4.41 | 0.06 | 0.09 | OD |
qew1-2 | 三坪 | 1/1.07 | 84 | 212.21~212.36 | RM1_173 | RM1_174 | 2.57 | 5.88 | 0.05 | -0.01 | A |
qew4-1 | 三坪 | 4/4.08 | 60 | 188.92~189.03 | RM4_135 | RM4_136 | 4.66 | 10.63 | 0.05 | 0.05 | D |
qew8-2 | 九圣禾 | 8/8.05 | 31 | 138.67~138.78 | RM8_63 | RM8_64 | 2.65 | 6.28 | 0.01 | 0.02 | OD |
qew1-3 | 联合 | 1/1.06 | 74 | 196.18~197.14 | RM1_149 | RM1_150 | 4.02 | 5.47 | 0.05 | -0.01 | A |
qew3-2 | 联合 | 3/3.04 | 28 | 36.82~50.97 | RM3_50 | RM3_51 | 3.93 | 5.21 | -0.05 | -0.01 | A |
qew4-2 | 联合 | 4/4.09 | 69 | 223.31~224.37 | RM4_155 | RM4_156 | 6.03 | 8.19 | 0.06 | 0.02 | PD |
qew5-2 | 联合 | 5/5.01 | 17 | 8.86~8.90 | RM5_27 | RM5_26 | 9.08 | 13.18 | 0.08 | 0.01 | A |
qew10-1 | 联合 | 10/10.07 | 56 | 146.71~146.77 | RM10_102 | RM10_103 | 2.78 | 3.75 | 0.04 | 0.0001 | A |
Tab.3 QTL mapping of leaf width in F2∶3 families
QTL | 环境 Environ ment | 染色体 /Bin Chromo- some /Bin | 遗传位置 Genetic position (cM) | 物理距离 /Mba Physical position /Mba | 左翼标记 Left marker | 右翼标记 Right marker | LOD值 | 表型 贡献率 PVE (%) | 加性 效应 Add | 显性 效应 Dom | 基因 效应b Gene action |
---|---|---|---|---|---|---|---|---|---|---|---|
qew1-1 | 海南 | 1/1.06 | 74 | 196.18~197.14 | RM1_149 | RM1_150 | 2.73 | 5.04 | 0.1 | -0.06 | PD |
qew3-1 | 海南 | 3/3.07 | 52 | 198.91~198.92 | RM3_117 | RM3_118 | 2.93 | 5.51 | -0.1 | -0.01 | A |
qew5-1 | 海南 | 5/5.01 | 17 | 8.86~8.90 | RM5_27 | RM5_26 | 8.06 | 16.17 | 0.2 | 0.01 | A |
qew8-1 | 海南 | 8/8.02 | 13 | 13.54~13.64 | RM8_22 | RM8_23 | 2.52 | 4.41 | 0.06 | 0.09 | OD |
qew1-2 | 三坪 | 1/1.07 | 84 | 212.21~212.36 | RM1_173 | RM1_174 | 2.57 | 5.88 | 0.05 | -0.01 | A |
qew4-1 | 三坪 | 4/4.08 | 60 | 188.92~189.03 | RM4_135 | RM4_136 | 4.66 | 10.63 | 0.05 | 0.05 | D |
qew8-2 | 九圣禾 | 8/8.05 | 31 | 138.67~138.78 | RM8_63 | RM8_64 | 2.65 | 6.28 | 0.01 | 0.02 | OD |
qew1-3 | 联合 | 1/1.06 | 74 | 196.18~197.14 | RM1_149 | RM1_150 | 4.02 | 5.47 | 0.05 | -0.01 | A |
qew3-2 | 联合 | 3/3.04 | 28 | 36.82~50.97 | RM3_50 | RM3_51 | 3.93 | 5.21 | -0.05 | -0.01 | A |
qew4-2 | 联合 | 4/4.09 | 69 | 223.31~224.37 | RM4_155 | RM4_156 | 6.03 | 8.19 | 0.06 | 0.02 | PD |
qew5-2 | 联合 | 5/5.01 | 17 | 8.86~8.90 | RM5_27 | RM5_26 | 9.08 | 13.18 | 0.08 | 0.01 | A |
qew10-1 | 联合 | 10/10.07 | 56 | 146.71~146.77 | RM10_102 | RM10_103 | 2.78 | 3.75 | 0.04 | 0.0001 | A |
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