Genetic Diversity of Wild Agaricus sinodeliciosus in Xinjiang

doi:10.6048/j.issn.1001-4330.2022.11.014

Abstract

Abstract:

【Objective】 To study the genetic diversity and genetic differentiation characteristics of Wild Agaricus sinodeliciosus in Xinjiang in the hope of providing basic materials and scientific basis for enriching and developing the germplasm resources of Agaricus sinodeliciosus and the breeding of new varieties. 【Methods】 Traditional biological method and ISSR marker technology were used to carry out the genetic diversity analysis on 20 wild Agaricus sinodeliciosus strains. 【Results】 In the mycelia culture characteristics, there were significant differences in many aspects including colony morphology and mycelium growth.16 ISSR primers amplified a total of 206 clear DNA bands, including 176 polymorphic bands with a polymorphism ratio of 83.50％. The results of cluster analysis showed that the genetic similarity coefficient of each strain ranged from 0.60 to 0.91, and the 20 tested strains were divided into 8 groups at the genetic similarity coefficient of 0.80. 【Conclusion】 The natural populations of wild Agaricus sinodeliciosus in Xinjiang has the occurrence of genetic differentiation with relatively rich genetic diversity and good domestication breeding potential.

Key words: Agaricus sinodeliciosus; cultural characteristic; ISSR; genetic diversity

摘要：

【目的】研究新疆野生中国美味蘑菇菌株的遗传多样性和遗传分化特征,为丰富和开发中国美味蘑菇种质资源及新品种选育提供基础材料和科学依据。【方法】利用生物学和ISSR标记技术对20个野生中国美味蘑菇菌株分析遗传多样性。【结果】中国美味蘑菇各菌株的菌落形态、菌丝长势等方面存在明显差异。16条ISSR引物共扩增出206条清晰的DNA条带,多态性条带176条,多态比率为83.50％。各菌株间遗传相似系数范围在 0.60~0.91,遗传相似系数为0.80时,可将20个供试菌株分为8个类群。【结论】新疆野生中国美味蘑菇菌株已开始发生遗传分化,并具有丰富的遗传多样性,具有较好的驯化育种潜力。

关键词: 中国美味蘑菇, 菌丝培养特征, 分子标记技术, 遗传多样性

CLC Number:

S567.3

LUO Ying, GUAN Yongqiang, JIA Peisong, Nurziya Yalimaimaiti, QI Haoxuan, ZHAO Zhenhao, WEI Peng, JIA Wenjie. Genetic Diversity of Wild Agaricus sinodeliciosus in Xinjiang[J]. Xinjiang Agricultural Sciences, 2022, 59(11): 2707-2713.

罗影, 贾培松, 努尔孜亚·亚力买买提, 祁颢萱, 赵振豪, 贾文捷. 新疆野生中国美味蘑菇的遗传多样性分析[J]. 新疆农业科学, 2022, 59(11): 2707-2713.

Figures/Tables 6

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序号 No.	引物编号 Primer No	引物序列 Primer sequence (5'-3')	退火温度 Annealing temperature (℃)
1	P1	TGCACACACACACAC	46
2	P2	GTGACACACACACAC	46
3	P5	CGTGTGTGTGTGTGT	46
4	P6	AGTGTGTGTGTGTGT	44
5	P8	GGAGTGGTGGTGGTG	50
6	P14	ACACACACACACACACCG	48
7	P18	ACACACACACACACACC	48
8	P21	AGCAGCAGCAGCAGCAGCG	48
9	P22	AAGAAGAAGAAGAAGAAGC	48
10	P25	GAGAGAGAGAGAGAGACC	50
11	ISSR1	CTCCTCCTCCTCSC	48
12	ISSR6	DHBCGACGACGACGACGA	48
13	ISSR12	AGAGAGAGAGAGAGAGT	48
14	ISSR13	GAGAGAGAGAGAGAGAC	48
15	ISSR18	VHVGTGTGTGTGTGTGTGT	48
16	ISSR808	AGAGAGAGAGAGAGAGC	48

序号 No.	引物编号 Primer No	引物序列 Primer sequence (5'-3')	退火温度 Annealing temperature (℃)
1	P1	TGCACACACACACAC	46
2	P2	GTGACACACACACAC	46
3	P5	CGTGTGTGTGTGTGT	46
4	P6	AGTGTGTGTGTGTGT	44
5	P8	GGAGTGGTGGTGGTG	50
6	P14	ACACACACACACACACCG	48
7	P18	ACACACACACACACACC	48
8	P21	AGCAGCAGCAGCAGCAGCG	48
9	P22	AAGAAGAAGAAGAAGAAGC	48
10	P25	GAGAGAGAGAGAGAGACC	50
11	ISSR1	CTCCTCCTCCTCSC	48
12	ISSR6	DHBCGACGACGACGACGA	48
13	ISSR12	AGAGAGAGAGAGAGAGT	48
14	ISSR13	GAGAGAGAGAGAGAGAC	48
15	ISSR18	VHVGTGTGTGTGTGTGTGT	48
16	ISSR808	AGAGAGAGAGAGAGAGC	48

菌株 Strain	原基形成 Primordium formation	菌落形态 Colony morphology	菌丝长势评分 Mycelial growth score	菌丝生长速度 Mycelial growth rate(mm/d)	差异显著性 Significant difference
菌株 Strain	原基形成 Primordium formation	菌落形态 Colony morphology	菌丝长势评分 Mycelial growth score	菌丝生长速度 Mycelial growth rate(mm/d)	0.05	0.01
BK01	-	菌丝浓密,边缘整齐	3	1.34±0.08	abc	AB
BK03	-	菌丝浓密,边缘整齐	3	1.43±0.09	abcd	ABCD
BK08	-	菌丝浓密,边缘整齐	3	1.38±0.09	ab	AB
BK10	-	菌丝浓密,边缘不整齐	3	1.26±0.07	abcd	AB
BK11	-	菌丝稀疏,边缘整齐	1	0.66±0.05	gh	EF
BK30	-	菌丝浓密,边缘整齐	3	1.35±0.10	abc	AB
BK33	-	菌丝稀疏,边缘不整齐	2	1.27±0.09	cdef	ABCDE
BK36	＋	菌丝一般,边缘整齐	3	1.22±0.04	abcd	ABC
BK38	-	菌丝一般,边缘整齐	2	0.94±0.08	defg	BCDEF
BK39	-	菌丝一般,边缘整齐	3	1.46±0.08	a	A
BK40	＋	菌丝一般,边缘整齐	3	1.04±0.05	bcdef	ABCDE
BK42	-	菌丝一般,边缘整齐	3	1.44±0.05	a	A
BK49	-	菌丝一般,边缘整齐	3	1.33±0.08	abc	AB
BK50	-	菌丝浓密,边缘不整齐	2	0.94±0.05	defg	BCDEF
BK51	-	菌丝浓密,边缘不整齐	1	0.22±0.05	-	G
BK52	＋	菌丝一般,边缘不整齐	3	0.80±0.04	efgh	CDEF
BK72	＋	菌丝一般,边缘整齐	3	0.76±0.06	h	FG
BK73	-	菌丝浓密,边缘不整齐	2	1.07±0.06	bcde	ABCDE
BK74	-	菌丝浓密,边缘不整齐	1	0.90±0.08	fgh	DEF
BK75	-	菌丝浓密,边缘整齐	3	1.22±0.09	abcd	ABC

菌株 Strain	原基形成 Primordium formation	菌落形态 Colony morphology	菌丝长势评分 Mycelial growth score	菌丝生长速度 Mycelial growth rate(mm/d)	差异显著性 Significant difference
菌株 Strain	原基形成 Primordium formation	菌落形态 Colony morphology	菌丝长势评分 Mycelial growth score	菌丝生长速度 Mycelial growth rate(mm/d)	0.05	0.01
BK01	-	菌丝浓密,边缘整齐	3	1.34±0.08	abc	AB
BK03	-	菌丝浓密,边缘整齐	3	1.43±0.09	abcd	ABCD
BK08	-	菌丝浓密,边缘整齐	3	1.38±0.09	ab	AB
BK10	-	菌丝浓密,边缘不整齐	3	1.26±0.07	abcd	AB
BK11	-	菌丝稀疏,边缘整齐	1	0.66±0.05	gh	EF
BK30	-	菌丝浓密,边缘整齐	3	1.35±0.10	abc	AB
BK33	-	菌丝稀疏,边缘不整齐	2	1.27±0.09	cdef	ABCDE
BK36	＋	菌丝一般,边缘整齐	3	1.22±0.04	abcd	ABC
BK38	-	菌丝一般,边缘整齐	2	0.94±0.08	defg	BCDEF
BK39	-	菌丝一般,边缘整齐	3	1.46±0.08	a	A
BK40	＋	菌丝一般,边缘整齐	3	1.04±0.05	bcdef	ABCDE
BK42	-	菌丝一般,边缘整齐	3	1.44±0.05	a	A
BK49	-	菌丝一般,边缘整齐	3	1.33±0.08	abc	AB
BK50	-	菌丝浓密,边缘不整齐	2	0.94±0.05	defg	BCDEF
BK51	-	菌丝浓密,边缘不整齐	1	0.22±0.05	-	G
BK52	＋	菌丝一般,边缘不整齐	3	0.80±0.04	efgh	CDEF
BK72	＋	菌丝一般,边缘整齐	3	0.76±0.06	h	FG
BK73	-	菌丝浓密,边缘不整齐	2	1.07±0.06	bcde	ABCDE
BK74	-	菌丝浓密,边缘不整齐	1	0.90±0.08	fgh	DEF
BK75	-	菌丝浓密,边缘整齐	3	1.22±0.09	abcd	ABC

序号 No.	引物编号 Primer No	扩增条带数 Total of bands (n)	多态性条带数 polymorphic bands (k)	多态位点比率 Fraction of polymorphic (p/100％)
1	P1	19	19	100.00
2	P2	13	10	76.92
3	P5	14	14	100.00
4	P6	12	10	83.33
5	P8	9	7	77.78
6	P14	17	15	88.24
7	P18	16	7	43.75
8	P21	13	11	84.62
9	P22	9	9	100.00
10	P25	13	12	92.31
11	ISSR1	10	8	80.00
12	ISSR6	12	10	83.33
13	ISSR12	13	11	84.62
14	ISSR13	14	11	78.57
15	ISSR18	10	8	80.00
16	ISSR808	12	10	83.33
总计Total		206	172	83.50
平均Average		12.88	10.75	83.46