Influence of Different NH4 +-N/NO3 --N Ratios on Wheat Seedling Antioxidase Activities under NaCl Stress

doi:10.6048/j.issn.1001-4330.2021.12.003

Abstract

Abstract:

【Objective】The purpose of this experiment is to explore the effective ways to reduce the damage of salt stress to wheat seedlings from nitrogen form supply. 【Methods】 A hydroponics experiment was carried out to investigate the influence of different NO₃ ^--N/NH₄ ⁺-N ratios application on wheat seedling antioxidase activities and membrane permeability under NaCl stress. 【Results】The results showed that under both salt stress and control conditions that wheat seedling plant biomass was significantly increased with increase of the ration of NH₄ ⁺-N/NO₃ ^--N supply, and wheat enzyme activities of superoxide dismutase (SOD), peroxidase (POX) and ascorbate peroxidase (APX) were significantly increased as well. Leaf membrane permeability significantly increased with the increase of NaCl concentration, however, root activity, enzyme activities of SOD, POX and APX were significantly decreased with the increase of NaCl concentration (P<0.05). 【Conclusion】The findings indicates that the higher NH₄ ⁺-N/NO₃ ^--N ratios (NH₄ ⁺-N /NO₃ ^--N, 75/25) and purely NH₄ ⁺-N could ameliorate the adverse effect resulting from stress-resistant, and attenuate the toxic effects of salt stress to a certain extent.

Key words: NaCl; NH₄ ⁺-N/NO₃ ^--N ratio; wheat; antioxidase activity

摘要： 目的研究分析减轻盐分胁迫对小麦幼苗伤害的影响。方法采用水培方法,研究NaCl胁迫下不同铵、硝配比对小麦苗期生长及抗氧化酶活性的影响。结果在盐分胁迫和非盐分胁迫条件下,相对于纯硝营养,增铵营养在一定程度上能够提高小麦生物量,提高小麦叶片抗氧化酶(SOD、POX和APX)活性,减轻盐分胁迫所导致的细胞膜脂过氧化作用,提高植物的抗逆境胁迫能力。随着盐分浓度的增加小麦叶片质膜透性显著增大,而根系活力、SOD、POX和APX活性则随着盐分浓度的增加显著降低 (P<0.05)。结论增铵营养在一定程度上对植物抵御盐分胁迫所造成的不利影响有明显的促进作用,并在一定程度上能够减轻盐分的毒害作用。

关键词: NaCl, 铵硝配比, 小麦, 抗氧化酶活性

CLC Number:

S512

QIAO Xu, Gao Yonghong, Sailihan Sai, XUE Lihua, ZHANG Yongqiang, CHEN Chuanxin, XIAO Li, LEI Junjie. Influence of Different NH₄ ⁺-N/NO₃ ^--N Ratios on Wheat Seedling Antioxidase Activities under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(12): 2176-2181.

乔旭, 高永红, 赛力汗·赛, 薛丽华, 张永强, 陈传信, 肖丽, 雷钧杰. NaCl胁迫下不同铵、硝配比对小麦抗氧化酶活性的影响[J]. 新疆农业科学, 2021, 58(12): 2176-2181.

Figures/Tables 4

Table 1 The effect of NaCl and NH4+/NO3- treatments on total dry weight of wheat seedlings(g/plant)

NaCl(mM)	NH₄⁺/NO₃^-
NaCl(mM)	0/100	25/75	50/50	75/25	100/0
0	1.34±0.09^c	1.43±0.06^bc	1.48±0.11^ab	1.56±0.04^a	1.60±0.04^a
100	1.11±0.07^d	1.20±0.09^cd	1.31±0.08^bc	1.39±0.06^ab	1.48±0.07^a
200	0.80±0.06^d	0.87±0.05^d	1.00±0.07^c	1.11±0.05^b	1.22±0.04^a

Fig.1 The effect of NaCl and NH4+/ NO3- treatments on root activity of wheat seedlings Note: Vertical bars indicate ±S.E. Bars with different letters are statistically different (P<0.05). The same as below

Fig.2 The effect of NaCl and NH4+/ NO3- treatments on electrolyte leakage of wheat seedlings

Fig.3 The effect of NaCl and NH4+/ NO3- treatments on the antioxidative enzyme activities of wheat seedlings

References 16

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Influence of Different NH₄ ⁺-N/NO₃ ^--N Ratios on Wheat Seedling Antioxidase Activities under NaCl Stress

NaCl胁迫下不同铵、硝配比对小麦抗氧化酶活性的影响

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