新疆农业科学 ›› 2023, Vol. 60 ›› Issue (9): 2239-2247.DOI: 10.6048/j.issn.1001-4330.2023.09.019
宋冰梅1,2(), 姜岩2, 陈鑫2,3, 张宇4, 程宛楠4, 潘洪生2()
收稿日期:
2022-12-10
出版日期:
2023-09-20
发布日期:
2023-09-19
通信作者:
潘洪生(1982-),男,黑龙江肇东人,研究员,博士,研究方向为转基因作物安全性评价,(E-mail)作者简介:
宋冰梅(1999-),女,四川自贡人,硕士研究生,研究方向为转基因作物安全性评价,(E-mail)2446620106@qq.com
基金资助:
SONG Bingmei1,2(), JIANG Yan2, CHEN Xin2,3, ZHANG Yu4, CHENG Wannan4, PAN Hongsheng2()
Received:
2022-12-10
Online:
2023-09-20
Published:
2023-09-19
Correspondence author:
PAN Hongsheng (1982-), male, native place: Zhaodong, Heilongjiang Province, researcher, master tutor, research direction for safety evaluation of transgenic crops, (E- mail) Supported by:
摘要:
【目的】研究盐/碱胁迫对新型转基因高产棉花萌发期和苗期生长发育的影响并对其耐盐性与耐碱性进行评价。【方法】以转Bn-csRRM2基因高产棉花HN9311和亲本对照中棉所12号(CCRI-12)为研究对象,在盐胁迫(150 mmol/L NaCl)和碱胁迫(70 mmol/L NaHCO3)下,统计和分析生长发育相关指标及差异。【结果】与对照相比,除真叶数和根冠比以外,在盐胁迫和碱胁迫下HN9311萌发期和苗期各项直接指标均显著下降;除萌发期碱胁迫下相对下胚轴长和相对发芽指数以及苗期碱胁迫下相对根冠比以外,HN9311萌发期和苗期各项相对指标均显著低于或等于中棉所12号;HN9311萌发期相对盐/碱害率分别为27.00%、21.00%,中棉所12号分别为22.75%、23.00%,HN9311相对盐害率显著大于中棉所12号。【结论】盐/碱胁迫对HN9311萌发期和苗期生长发育均具有显著的抑制作用,与盐胁迫相比其苗期对碱胁迫更为敏感,萌发期耐盐胁迫能力和苗期耐盐/碱胁迫能力均低于中棉所12号。
中图分类号:
宋冰梅, 姜岩, 陈鑫, 张宇, 程宛楠, 潘洪生. 新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价[J]. 新疆农业科学, 2023, 60(9): 2239-2247.
SONG Bingmei, JIANG Yan, CHEN Xin, ZHANG Yu, CHENG Wannan, PAN Hongsheng. Evaluation of saline/alkali tolerance of new transgenic High-Yield cotton at germination and seedling stages[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2239-2247.
直接指标 Direct index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
0 mmol/L NaCl (CK) | 150 mmol/L NaCl | 0 mmol/L NaHCO3 (CK) | 70 mmol/L NaHCO3 | |
鲜重量Fresh weight(g) | 0.36±0.01a | 0.26±0.01b | 0.36±0.01a | 0.26±0.01b |
下胚轴长Hypocotyl length(cm) | 1.72±0.05a | 1.41±0.14b | 1.70±0.03a | 1.37±0.02b |
根长Root length(cm) | 4.43±0.05a | 2.22±0.08b | 4.31±0.04a | 0.00±0.00c |
发芽势Germination potential(%) | 66.25±0.48a | 27.50±0.29c | 67.50±0.29a | 43.00±0.91b |
发芽率Germination rate(%) | 98.25±0.48a | 72.00±0.82c | 98.25±0.48a | 78.00±0.00b |
发芽指数Germination index | 95.42±0.19a | 56.40±0.48c | 95.85±0.13a | 71.04±0.33b |
活力指数Vigor index | 34.26±0.59a | 14.92±0.68c | 33.80±0.62a | 18.03±0.40b |
表1 盐/碱胁迫下新型转基因高产棉花HN9311萌发期直接指标变化
Tab.1 Effects of saline/alkali stress on the direct index of new transgenic high-yield cotton HN9311 at germination stage
直接指标 Direct index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
0 mmol/L NaCl (CK) | 150 mmol/L NaCl | 0 mmol/L NaHCO3 (CK) | 70 mmol/L NaHCO3 | |
鲜重量Fresh weight(g) | 0.36±0.01a | 0.26±0.01b | 0.36±0.01a | 0.26±0.01b |
下胚轴长Hypocotyl length(cm) | 1.72±0.05a | 1.41±0.14b | 1.70±0.03a | 1.37±0.02b |
根长Root length(cm) | 4.43±0.05a | 2.22±0.08b | 4.31±0.04a | 0.00±0.00c |
发芽势Germination potential(%) | 66.25±0.48a | 27.50±0.29c | 67.50±0.29a | 43.00±0.91b |
发芽率Germination rate(%) | 98.25±0.48a | 72.00±0.82c | 98.25±0.48a | 78.00±0.00b |
发芽指数Germination index | 95.42±0.19a | 56.40±0.48c | 95.85±0.13a | 71.04±0.33b |
活力指数Vigor index | 34.26±0.59a | 14.92±0.68c | 33.80±0.62a | 18.03±0.40b |
直接指标 Direct index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
0 mmol/L NaCl (CK) | 150 mmol/L NaCl | 0 mmol/L NaHCO3 (CK) | 70 mmol/L NaHCO3 | |
真叶数Number of true leaves | 3.75±0.25ab | 3.25±0.25b | 4.00±0.00a | 2.25±0.08c |
株高Plant height(cm) | 15.00±0.41a | 14.00±0.00b | 15.80±0.15a | 9.93±0.38c |
根长Root length(cm) | 21.63±0.38a | 14.00±0.41b | 21.70±0.10a | 9.83±0.94c |
根鲜重Root fresh weight(g) | 2.57±0.02a | 1.93±0.05b | 2.55±0.05a | 1.56±0.05c |
地上部鲜重Shoot fresh weight(g) | 5.28±0.06a | 3.33±0.06b | 5.23±0.03a | 2.10±0.10c |
鲜重量Fresh weight(g) | 7.85±0.04a | 5.25±0.10b | 7.78±0.09a | 3.66±0.15c |
根冠比Root/shoot | 0.49±0.01c | 0.58±0.01b | 0.49±0.01c | 0.74±0.01a |
表2 盐/碱胁迫下新型转基因高产棉花HN9311苗期直接指标变化
Tab.2 Effects of saline/alkali stress on the direct index of new transgenic high-yield cotton HN9311 at seedling stage
直接指标 Direct index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
0 mmol/L NaCl (CK) | 150 mmol/L NaCl | 0 mmol/L NaHCO3 (CK) | 70 mmol/L NaHCO3 | |
真叶数Number of true leaves | 3.75±0.25ab | 3.25±0.25b | 4.00±0.00a | 2.25±0.08c |
株高Plant height(cm) | 15.00±0.41a | 14.00±0.00b | 15.80±0.15a | 9.93±0.38c |
根长Root length(cm) | 21.63±0.38a | 14.00±0.41b | 21.70±0.10a | 9.83±0.94c |
根鲜重Root fresh weight(g) | 2.57±0.02a | 1.93±0.05b | 2.55±0.05a | 1.56±0.05c |
地上部鲜重Shoot fresh weight(g) | 5.28±0.06a | 3.33±0.06b | 5.23±0.03a | 2.10±0.10c |
鲜重量Fresh weight(g) | 7.85±0.04a | 5.25±0.10b | 7.78±0.09a | 3.66±0.15c |
根冠比Root/shoot | 0.49±0.01c | 0.58±0.01b | 0.49±0.01c | 0.74±0.01a |
相对指标 Relative index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
中棉所12号 CCRI-12 | HN9311 | 中棉所12号 CCRI-12 | HN9311 | |
相对鲜重量Relative fresh weight | 0.86±0.02* | 0.74±0.03 | 0.95±0.0 | 0.72±0.02 |
相对下胚轴长Relative hypocotyl length | 0.98±0.02 | 0.82±0.08 | 0.73±0.01 | 0.81±0.0 |
相对根长Relative root length | 0.56±0.03 | 0.50±0.02 | 0.28±0.00 | 0.28±0.01 |
相对发芽势Relative germination potential | 0.56±0.0 | 0.42±0.00 | 0.65±0.01 | 0.64±0.01 |
相对发芽率Relative germination rate | 0.77±0.0 | 0.73±0.01 | 0.78±0.00 | 0.79±0.00 |
相对发芽指数Relative germination index | 0.70±0.0 | 0.59±0.00 | 0.72±0.00 | 0.74±0.00* |
相对活力指数 Relative vigor index | 0.60±0.0 | 0.43±0.02 | 0.68±0.01 | 0.53±0.01 |
表3 盐/碱胁迫下新型转基因高产棉花HN9311萌发期相对指标变化
Tab.3 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at germination stage
相对指标 Relative index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
中棉所12号 CCRI-12 | HN9311 | 中棉所12号 CCRI-12 | HN9311 | |
相对鲜重量Relative fresh weight | 0.86±0.02* | 0.74±0.03 | 0.95±0.0 | 0.72±0.02 |
相对下胚轴长Relative hypocotyl length | 0.98±0.02 | 0.82±0.08 | 0.73±0.01 | 0.81±0.0 |
相对根长Relative root length | 0.56±0.03 | 0.50±0.02 | 0.28±0.00 | 0.28±0.01 |
相对发芽势Relative germination potential | 0.56±0.0 | 0.42±0.00 | 0.65±0.01 | 0.64±0.01 |
相对发芽率Relative germination rate | 0.77±0.0 | 0.73±0.01 | 0.78±0.00 | 0.79±0.00 |
相对发芽指数Relative germination index | 0.70±0.0 | 0.59±0.00 | 0.72±0.00 | 0.74±0.00* |
相对活力指数 Relative vigor index | 0.60±0.0 | 0.43±0.02 | 0.68±0.01 | 0.53±0.01 |
相对指标 Relative index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
中棉所12号 CCRI-12 | HN9311 | 中棉所12号 CCRI-12 | HN9311 | |
相对真叶数Relative true leaf number | 0.89±0.00 | 0.87±0.07 | 0.56±0.02 | 0.60±0.02 |
相对株高Relative plant height | 0.95±0.03 | 0.93±0.00 | 0.77±0.01* | 0.66±0.03 |
相对根长Relative root length | 0.60±0.01 | 0.65±0.02 | 0.59±0.03 | 0.46±0.04 |
相对根鲜重Relative root fresh weight | 0.90±0.0 | 0.75±0.02 | 0.86±0.0 | 0.61±0.02 |
相对地上部鲜重Relative shoot fresh weight | 0.87±0.07* | 0.63±0.01 | 0.75±0.0 | 0.40±0.02 |
相对鲜重量Relative fresh weight | 0.88±0.0 | 0.67±0.01 | 0.79±0.0 | 0.47±0.02 |
相对根冠比Relative root/shoot | 1.06±0.09 | 1.19±0.03 | 1.14±0.01 | 1.53±0.0 |
表4 盐/碱胁迫下新型转基因高产棉花HN9311苗期相对指标变化
Tab.4 Effects of saline/alkali stress on the relative index of new transgenic high-yield cotton HN9311 at seedling stage
相对指标 Relative index | 盐胁迫 Saline stress | 碱胁迫 Alkali stress | ||
---|---|---|---|---|
中棉所12号 CCRI-12 | HN9311 | 中棉所12号 CCRI-12 | HN9311 | |
相对真叶数Relative true leaf number | 0.89±0.00 | 0.87±0.07 | 0.56±0.02 | 0.60±0.02 |
相对株高Relative plant height | 0.95±0.03 | 0.93±0.00 | 0.77±0.01* | 0.66±0.03 |
相对根长Relative root length | 0.60±0.01 | 0.65±0.02 | 0.59±0.03 | 0.46±0.04 |
相对根鲜重Relative root fresh weight | 0.90±0.0 | 0.75±0.02 | 0.86±0.0 | 0.61±0.02 |
相对地上部鲜重Relative shoot fresh weight | 0.87±0.07* | 0.63±0.01 | 0.75±0.0 | 0.40±0.02 |
相对鲜重量Relative fresh weight | 0.88±0.0 | 0.67±0.01 | 0.79±0.0 | 0.47±0.02 |
相对根冠比Relative root/shoot | 1.06±0.09 | 1.19±0.03 | 1.14±0.01 | 1.53±0.0 |
图1 盐/碱胁迫下新型转基因高产棉花HN9311萌发期相对盐/碱害率变化 注:*、ns分别代表HN9311与中棉所12号相对盐害率具有(P<0.05)或不具有(P>0.05)显著性差异(独立样本T检验法检验)
Fig.1 Effects of saline/alkali stress on relative saline/alkali damage rate of new transgenic high-yield cotton HN9311 at germination stage Note:* and ns respectively represent significant difference (P<0.05) or not represent significant difference (P>0.05) for the relative saline damage rate between HN9311 and CCRI-12 (independent sample T test)
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