新疆农业科学 ›› 2023, Vol. 60 ›› Issue (5): 1041-1049.DOI: 10.6048/j.issn.1001-4330.2023.05.001
• 新疆主要粮食作物绿色丰产提质增效专栏 • 上一篇 下一篇
张燕红1(), 侯天钰1, 巴音花1, 赵彩月1, 吕玉平2, 布哈丽且木·阿不力孜1, 赵志强1, 李冬2, 杜孝敬2, 袁杰1, 王奉斌2()
收稿日期:
2022-09-05
出版日期:
2023-05-20
发布日期:
2023-05-22
通信作者:
王奉斌(1968-),男,江西人,研究员,硕士,研究方向为水稻遗传育种及栽培,(E-mail)作者简介:
张燕红(1982-),女,甘肃武威人,副研究员,研究方向为水稻遗传育种,(E-mail)zhangyanhong9527@163.com
基金资助:
ZHANG Yanhong1(), HOU Tianyu1, BA Yinhua1, ZHAO Caiyue1, LYU Yuping2, Buhalikeimu Abunzi1, ZHAO Zhiqiang1, LI Dong2, DU Xiaojing2, YUAN Jie1, WANG Fengbin2()
Received:
2022-09-05
Online:
2023-05-20
Published:
2023-05-22
Correspondence author:
WANG Fengbin (1968-), male, born in Jiangxi Province, master degree, researcher, engaged in rice genetic breeding and cultivation technology, (E-mail)Supported by:
摘要:
【目的】鉴定水稻种质资源芽期和苗期耐盐性,筛选耐盐性优良的种质资源,为挖掘水稻耐盐种质和培育耐盐新品种提供种质资源和参考依据。【方法】采用前期构建稳定的219份重组自交系为试验材料,在水稻的芽期和苗期分别利用1.2% NaCl和0.7% NaCl溶液鉴定和评价耐盐性。【结果】芽期用1.2% NaCl处理后发芽势和发芽率显著降低,筛选出4份耐盐材料和6份敏感材料。耐盐材料的发芽势、发芽率、芽长、根长下降缓慢,敏感材料发芽势、发芽率、芽长、根长显著下降。苗期用0.7% NaCl处理后,群体材料的存活率显著降低;耐盐材料和敏感材料的表型比较发现,耐盐材料株高和存活率有所下降但未达到显著水平,敏感材料株高和存活率显著下降,耐盐材料相比敏感材料受抑制程度较轻。【结论】219份材料的芽期受到盐胁迫后,相对发芽势、相对发芽率和存活率显著降低;耐盐材料和敏感材料的耐盐性不同,耐盐材料受盐胁迫的程度低于敏感材料;筛选出芽期耐盐性强材料18份;苗期耐盐性强材料2份。
中图分类号:
张燕红, 侯天钰, 巴音花, 赵彩月, 吕玉平, 布哈丽且木·阿不力孜, 赵志强, 李冬, 杜孝敬, 袁杰, 王奉斌. 水稻重组自交系群体芽期和苗期耐盐性鉴定与评价[J]. 新疆农业科学, 2023, 60(5): 1041-1049.
ZHANG Yanhong, HOU Tianyu, BA Yinhua, ZHAO Caiyue, LYU Yuping, Buhalikeimu Abunzi, ZHAO Zhiqiang, LI Dong, DU Xiaojing, YUAN Jie, WANG Fengbin. Identification and evaluation of salt tolerance of rice recombinant inbred lines at bud and seedling stages[J]. Xinjiang Agricultural Sciences, 2023, 60(5): 1041-1049.
评价级别 Rating | 耐盐指数 Salt tolerant coefficient | 耐盐性 Salt tolerant ability |
---|---|---|
1 | ≤20 | 高耐 |
3 | 20.1~40.0 | 耐盐 |
5 | 40.1~60.0 | 中 |
7 | 60.1~80.0 | 敏感 |
9 | 80.1~100.0 | 高敏 |
表1 水稻发芽期耐盐性评价标准
Tab.1 Salt tolerance evaluation criteria of rice during germination
评价级别 Rating | 耐盐指数 Salt tolerant coefficient | 耐盐性 Salt tolerant ability |
---|---|---|
1 | ≤20 | 高耐 |
3 | 20.1~40.0 | 耐盐 |
5 | 40.1~60.0 | 中 |
7 | 60.1~80.0 | 敏感 |
9 | 80.1~100.0 | 高敏 |
评价级别 Rating | 盐害症状 Symptoms of salt damage | 耐盐性 Salt tolerant ability |
---|---|---|
1 | 生长分蘖基本正常,叶片无受害症状 | 高耐 |
3 | 生长分蘖近正常,但叶尖或上部叶片1 /2发 白或卷曲;或生长分蘖受抑制,有些叶片卷曲 | 耐盐 |
5 | 生长分蘖严重抑制,多数叶片卷曲, 仅少数叶片伸长 | 中 |
7 | 生长分蘖停止,多数叶片干枯 | 敏感 |
9 | 植株死亡或接近死亡 | 高敏 |
表2 水稻苗期耐盐性评价标准
Tab.2 Salt tolerance evaluation criteria at seedling stage of rice
评价级别 Rating | 盐害症状 Symptoms of salt damage | 耐盐性 Salt tolerant ability |
---|---|---|
1 | 生长分蘖基本正常,叶片无受害症状 | 高耐 |
3 | 生长分蘖近正常,但叶尖或上部叶片1 /2发 白或卷曲;或生长分蘖受抑制,有些叶片卷曲 | 耐盐 |
5 | 生长分蘖严重抑制,多数叶片卷曲, 仅少数叶片伸长 | 中 |
7 | 生长分蘖停止,多数叶片干枯 | 敏感 |
9 | 植株死亡或接近死亡 | 高敏 |
图1 不同处理下群体材料发芽势、发芽率比较 注:不同小写字母表示相同材料在不同处理下差异显著(P<0.05),下同
Fig.1 Comparison of germination potential and germination rate of population materials under different treatments Note: Different lowercase letters indicate significant differences in the same material under different treatments (P<0.05), the same as below
性状 Trait | 处理 Treatment | 平均值 Mean | 最大值 Max | 最小值 Min | 极差 Range | 标准差 SD | 变异系数 CV(%) |
---|---|---|---|---|---|---|---|
发芽势 Germination potential (%) | CK | 87.9 | 99.2 | 31.4 | 67.8 | 10.5 | 11.9 |
NaCl | 4.5 | 66.3 | 0.0 | 66.3 | 7.5 | 169.2 | |
相对发芽势 Relative germination potential(%) | 4.9 | 68.5 | 0.0 | 68.5 | 8.0 | 163.8 | |
发芽率 Germination rate (%) | CK | 96.8 | 99.4 | 82.2 | 17.2 | 3.1 | 3.2 |
NaCl | 27.4 | 92.2 | 0.0 | 92.2 | 19.4 | 70.8 | |
相对发芽率 Relative germination rate(%) | 28.2 | 93.1 | 0.0 | 93.1 | 19.8 | 70.3 | |
发芽势相对盐害率 Germination potential relative salt damage rate(%) | 73.9 | 100.0 | 32.0 | 68.0 | 8.0 | 16.7 | |
发芽率相对盐害率 Germination rate relative to salt damage rate(%) | 48.1 | 98.3 | 21.3 | 78.7 | 18.2 | 24.7 |
表3 219份水稻群体材料盐胁迫下芽期性状指标
Tab.3 Seedling stage traits of 219 rice population materials under salt stress
性状 Trait | 处理 Treatment | 平均值 Mean | 最大值 Max | 最小值 Min | 极差 Range | 标准差 SD | 变异系数 CV(%) |
---|---|---|---|---|---|---|---|
发芽势 Germination potential (%) | CK | 87.9 | 99.2 | 31.4 | 67.8 | 10.5 | 11.9 |
NaCl | 4.5 | 66.3 | 0.0 | 66.3 | 7.5 | 169.2 | |
相对发芽势 Relative germination potential(%) | 4.9 | 68.5 | 0.0 | 68.5 | 8.0 | 163.8 | |
发芽率 Germination rate (%) | CK | 96.8 | 99.4 | 82.2 | 17.2 | 3.1 | 3.2 |
NaCl | 27.4 | 92.2 | 0.0 | 92.2 | 19.4 | 70.8 | |
相对发芽率 Relative germination rate(%) | 28.2 | 93.1 | 0.0 | 93.1 | 19.8 | 70.3 | |
发芽势相对盐害率 Germination potential relative salt damage rate(%) | 73.9 | 100.0 | 32.0 | 68.0 | 8.0 | 16.7 | |
发芽率相对盐害率 Germination rate relative to salt damage rate(%) | 48.1 | 98.3 | 21.3 | 78.7 | 18.2 | 24.7 |
图3 水稻群体芽期和苗期耐盐性级别分布 注:a:芽期;b:苗期
Fig.3 Distribution of salt tolerance levels at bud and seedling stages of rice population Note: a:the bud stage, b:the seedling stage
图4 不同处理下耐盐和敏感材料生长比较 注: A:耐盐材料,B:敏感材料,下同
Fig.4 Comparison of growth conditions of salt-tolerant and sensitive materials under different treatments Note:A: salt-resistant material, B: sensitive material, the same as below
图5 盐胁迫处理下不同耐盐性材料发芽势、发芽率比较 注:不同大写字母代表相同处理下不同材料差异显著,不同小写字母代表相同材料在不同处理下差异显著(P<0.05),下同
Fig.5 Comparison of germination potential and germination rate of different salt-tolerant materials under salt stress Note: The same uppercase letter means significant difference between different materials under the same treatment, and different lowercase letter means significant difference between the same materials under different treatment (P<0.05), the same as below
[1] | 赵可夫, 范海. 盐生植物及其对盐渍生境的适应生理[M]. 北京: 科学技术出版社, 2005. |
ZHAO Kefu, FAN Hai. Halophytes and Their Adaptive Physiology to Saline Habitat[M]. Beijing: Science and Technology Press, 2005. | |
[2] |
宋志平, 陈家宽, 赵耀. 水稻驯化与长江文明[J]. 生物多样性, 2018, 26(4): 346-356.
DOI |
SONG Zhiping, CHEN Jiakuan, ZHAO Yao. Rice domestication and Yangtze River Civilization[J]. Biodiversity Science, 2018, 26(4): 346-356.
DOI |
|
[3] | 邵华伟, 孙九胜, 胡伟, 等. 新疆盐碱地分布特点和成因及改良利用技术研究进展[J]. 黑龙江农业科学, 2014,(11):160-164. |
SHAO Huawei, SUN Jiusheng, HU Wei, et al. Research progress on distribution characteristics, causes and improved utilization technology of saline-alkali land in Xinjiang[J]. Heilongjiang Agricultural Sciences, 2014,(11):160-164. | |
[4] | 方先文, 汤陵华, 王艳平. 耐盐水稻种质资源的筛选[J]. 植物遗传资源学报, 2004, 5(3):295-298. |
FANG Xianwen, TANG Linhua, WANG Yanping. Screening of salt-tolerant rice Germplasm resources[J]. Journal of Plant Genetic Resources, 2004, 5(3):295-298. | |
[5] |
WANG Z F, WANG J F, BAO Y M, et al. Quantitative trait loci controlling rice seed germination under salt stress[J]. Euphytica, 2011, 178(3): 297-307.
DOI URL |
[6] |
肖文斐, 马华升, 陈文岳, 等. 籼稻耐盐性与稻米品质性状的关联分析[J]. 核农学报, 2013, 27(12): 1938-1947.
DOI |
XIAO Wenfei, MA Huasheng, CHEN Wenyue, et al. Correlation analysis of salt tolerance and rice quality characters in indica rice[J]. Journal of Nuclear Agriculture, 2013, 27(12): 1938-1947. | |
[7] | 谢留杰, 段敏, 潘晓飚, 等. 不同类型水稻品系苗期和全生育期耐盐性鉴定与分析[J]. 江西农业大学学报, 2015, 37(3):404-410. |
XIE Liujie, DUAN Min, PAN Xiaobiao, et al. Identification and analysis of salt tolerance of different rice strains at seedling stage and whole growth stage[J]. Journal of Jiangxi Agricultural University, 2015, 37(3): 404-410. | |
[8] |
WANG Z F, WANG J F, BAO Y M, et al. Inheritance of rice seed germination ability under salt stress[J]. Rice Science, 2010, 17(2): 105-110.
DOI URL |
[9] |
CHEN D F, LI Y L, FANG T, et al. Specific roles of tocopherols and tocotrienols in seed longevity and germination tolerance to abiotic stress in transgenic rice[J]. Plant Science, 2016, 244: 31-39.
DOI PMID |
[10] | 夏秀忠, 张宗琼, 杨行海, 等. 广西地方稻种资源核心种质的耐盐性鉴定评价[J]. 南方农业学报, 2017, 48(6): 979-984. |
XIA Xiuzhong, ZHANG Zongqiong, YANG Xinhai, et al. Identification and evaluation of salt tolerance of core germplasm of local rice seed resources in Guangxi[J]. Journal of Southern Agricultural Sciences, 2017, 48(6): 979-984. | |
[11] |
田蕾, 陈亚萍, 刘俊, 等. 粳稻种质资源芽期耐盐性综合评价与筛选. 中国水稻科学, 2017, 31(6): 631-642.
DOI |
TIAN Lei, CHEN Yaping, LIU Jun, et al. Comprehensive evaluation and screening of salt tolerance at bud stage of japonica rice germplasm resources[J]. Chinese Journal of Rice Science, 2017, 31(6): 631-642.
DOI |
|
[12] |
Khan M.A., Abdullah Z. Salinity-sodicity induced changes in reproductive physiology of rice (Oryza sativa) under dense soil conditions[J]. Environmental and Experimental Botany, 2003, 49(2): 145-157.
DOI URL |
[13] | LEE S Y, AHN J H, CHA Y S, et al. Mapping QTLs related to salinity tolerance of rice at the young seedling stage[J]. Plant Breeding, 2007. |
[14] | 张燕红, 袁杰, 王奉斌. 不同新疆水稻品种种子发芽耐盐性的比较[J]. 安徽农学通报, 2009, 15(3): 102-103. |
ZHANG Yanhong, YUAN Jie, WANG Fengbin. Comparison of seed germination salt tolerance of different Xinjiang rice varieties[J]. Anhui Agricultural Science Bulletin, 2009, 15(3): 102-103. | |
[15] | 赵红, 徐芬芬, 熊安琪, 等. 不同种类盐胁迫对水稻种子萌发和幼苗生长的影响[J]. 分子植物育种, 2021, 19(17): 5842-5847. |
ZHAO Hong, XU Fenfen, XIONG Anqi, et al. Effects of different kinds of salt stress on seed germination and seedling growth of rice[J]. Molecular Plant Breeding, 2021, 19(17): 5842-5847. | |
[16] | 朱丽伟, 曹栋栋, 付玉营, 等. 可溶性寡糖和小分子的热激蛋白与杂交水稻种子成熟过程中发芽能力及种子活力相关[J]. 作物学报, 2016, 42(5): 714-724. |
ZHU Liwei, CAO Dongdong, FU Yuying, et al. Soluble oligo saccharides and small molecular heat shock proteins were associated with germination ability and seed vigor during the maturation of hybrid rice seeds[J]. Crops, 2016, 42(5): 714-724. | |
[17] | 鱼小军, 肖红, 徐长林, 等. 扁蓿豆和苜蓿种子萌发期抗旱性和耐盐性比较[J]. 植物遗传资源学报, 2015, 16(2): 405-410. |
YU Xiaojun, XIAO Hong, XU Changlin, et al. Comparison of drought resistance and salt tolerance of seeds of Ruthenica ruthenica and alfalfa during germination[J]. Journal of Plant Genetic Resources, 2015, 16(2): 405-410. | |
[18] | 李振, 廖同庆, 冯青春. 基于图像处理技术的黄瓜种子活力指数检测系统设计[J]. 种子, 2015, 34(6): 111-115. |
LI Zhen, LIAO Tongqin, FENG Qinchun. Design of cucumber seed vigor index detection system based on image processing technology[J]. Seed, 2015, 34(6): 111-115. | |
[19] | 郑崇珂, 张治振, 周冠华, 等. 不同水稻品种发芽期耐盐性评价[J]. 山东农业科学, 2018, 50(10):38-42. |
ZHENG Chongke, ZHANG Zhizhen, ZHOU Guanhua, et al. Evaluation of salt tolerance of different rice varieties at germination stage[J]. Shandong Agricultural Sciences, 2018, 50(10):38-42. | |
[20] | 郭望模, 傅亚萍, 孙宗修, 等. 盐胁迫下不同水稻种质形态指标与耐盐性的相关分析[J]. 植物遗传资源学报, 2003, 4(3): 245-251. |
GUO Wangmo, FU Yaping, SUN Zongxiu, et al. Correlation analysis of morphological indexes and salt tolerance of different rice germplasm under salt stress[J]. Journal of Plant Genetic Resources, 2003, 4(3): 245-251. | |
[21] |
肖文斐, 马华升, 陈文岳, 等. 籼稻耐盐性与稻米品质性状的关联分析[J]. 核农学报, 2013, 27(12): 1938-1947.
DOI |
XIAO Wenfei, MA Hhuasheng, CHEN Wenyue, et al. Association analysis between salt tolerance and rice quality in indica rice[J]. Journal of Nuclear Agronomy, 2013, 27(12): 1938-1947. | |
[22] | 贾宝艳, 周婵婵, 孙晓雪, 等. 辽宁省水稻种质资源的耐盐性鉴定评价[J]. 作物杂志, 2013, 4: 57-62. |
JIA Baoyan, ZHOU Chanchan, SUN Xiaoxue, et al. Evaluation of salt tolerance of rice germplasm resources in Liaoning Province[J]. Crops Journal, 2013, 4: 57-62. | |
[23] | 崔江慧, 谢登磊, 常金华. 高粱材料耐盐性综合评价方法的初步建立与验证[J]. 植物遗传资源学报, 2012, 13(1): 35-41. |
CUI Jianghui, XIE Denglei, CHANG Jinhua. Preliminary establishment and verification of a comprehensive evaluation method for salt tolerance of sorghum materials[J]. Journal of Plant Genetic Resources, 2012, 13(1): 35-41. | |
[24] | 张治振, 李稳, 周起先, 等. 不同水稻品种幼苗期耐盐性评价[J]. 作物杂志, 2020, (3): 92-101. |
ZHANG Zhizhen, Li Wen, ZHOU Qixian, et al. Evaluation of salt tolerance of different rice varieties at seedling stage[J]. Crops, 2020, (3): 92-101. | |
[25] | 刘佳音, 邵晓宇, 邹丹丹, 等. 水稻耐盐碱鉴定方法及评价指标研究进展[J]. 杂交水稻, 2019, 34(6): 1-6. |
LIU Jiayin, SHAO Xiaoyu, ZOU Dandan, et al. Research progress on methods and evaluation indexes of salt and alkali tolerance of rice[J]. Hybrid Rice, 2019, 34(6): 1-6. | |
[26] |
吴家富, 杨博文, 向珣朝, 等. 不同水稻种质在不同生育期耐盐鉴定的差异[J]. 植物学报, 2017, 52(1): 77-88.
DOI |
WU Jiafu, YANG Bowen, XIANG Xunchao, et al. Differences in salt tolerance of different rice germplasm at different growth stages[J]. Journal of Integrative Plant Biology, 2017, 52(1): 77-88.
DOI URL |
|
[27] | 马帅国, 田蓉蓉, 胡慧, 等. 粳稻种质资源苗期耐盐性综合评价与筛选[J]. 植物遗传资源学报, 2020, 21(5): 1089-1101. |
MA Shuaiguo, TIAN Rongrong, HU Hui, et al. Comprehensive evaluation and screening of salt tolerance of japonica rice germplasm resources at seedling stage[J]. Journal of Plant Genetic Resources, 2020, 21(5): 1099-1101. |
[1] | 王朋, 郑凯, 赵杰银, 高文举, 龙遗磊, 陈全家, 曲延英. 陆地棉种质资源材料的耐热性评价及指标筛选[J]. 新疆农业科学, 2023, 60(9): 2081-2090. |
[2] | 刘玉芳, 张志刚, 李长城, 李宏, 程平, 杨璐. 不同温度和成熟度对杏贮藏期腐烂率和品质的影响[J]. 新疆农业科学, 2023, 60(9): 2189-2197. |
[3] | 宋冰梅, 姜岩, 陈鑫, 张宇, 程宛楠, 潘洪生. 新型转基因高产棉花萌发期和苗期耐盐性与耐碱性评价[J]. 新疆农业科学, 2023, 60(9): 2239-2247. |
[4] | 曲可佳, 时晓磊, 张恒, 王兴州, 耿洪伟, 丁孙磊, 张金波, 严勇亮. PEG处理下引进春小麦品种苗期抗旱性评价[J]. 新疆农业科学, 2023, 60(6): 1363-1371. |
[5] | 陈艳, 黄璐瑶, 邓昌蓉, 张彦君, 侯全刚, 邵登魁. 冷害对多茸毛型线辣椒幼苗生理水平的影响[J]. 新疆农业科学, 2023, 60(6): 1492-1498. |
[6] | 哈力旦·依克热木, 刘娜, 刘联正, 周安定, 姜奇彦, 达买力江·合孜尔, 曹俊梅, 张新忠. 小麦近缘种芽期和苗期的耐盐性鉴定与评价[J]. 新疆农业科学, 2023, 60(5): 1118-1126. |
[7] | 姚庆, 阿里别里根·哈孜太, 杨明花, 李强, 苗昊翠, 崔宏亮. 藜麦种子对萌发温度的响应及低温胁迫萌发能力鉴定[J]. 新疆农业科学, 2023, 60(5): 1141-1149. |
[8] | 杨明花, 刘强, 廖必勇, 彭云承, 布阿依夏木·那曼提, 达吾来·杰克山. 不完全双列杂交玉米组合抗倒伏综合评价[J]. 新疆农业科学, 2023, 60(4): 832-840. |
[9] | 陈丽靓, 鲁倩君, 马媛媛, 刘迎, 赵宝龙, 孙军利. 不同葡萄品种的耐盐性比较分析[J]. 新疆农业科学, 2023, 60(4): 880-888. |
[10] | 赵斌荣, 田晓曦, 柴军发, 景亮亮, 张红艳, 洪波, 贾彦霞. 西蓝花田桃蚜的室内药剂筛选及田间药效评价[J]. 新疆农业科学, 2023, 60(3): 547-554. |
[11] | 侯良忠, 郭同军, 张俊瑜, 苏玲玲, 古再丽努尔·艾麦提, 温小燕, 朱晓芳, 杨建中, 王文奇. 不同比例籽用西葫芦皮瓤与玉米秸秆混贮效果评价[J]. 新疆农业科学, 2023, 60(3): 567-573. |
[12] | 陈亮亮, 张梦, 郭立平, 戚廷香, 张学贤, 唐会妮, 王海林, 乔秀琴, 吴建勇, 邢朝柱. 陆地棉杂交组合F1、F2苗期优势表现及亲本配合力分析[J]. 新疆农业科学, 2023, 60(2): 261-271. |
[13] | 马旭, 赵英, 韩炜, 武胜利, 韩晓燕. 14种沙棘果实中氨基酸组成的主成分分析与综合评价[J]. 新疆农业科学, 2023, 60(2): 378-388. |
[14] | 龙天宇, 邓亚辉, 祖倩丽, 杨龙, 曲延英, 陈全家. 棉花枯萎病室内苗期抗病性鉴定方法及评价[J]. 新疆农业科学, 2023, 60(2): 416-423. |
[15] | 刘旭坤, 阿尔孜姑丽·肉孜, 朱晓峰, 吐尔逊·阿合买提, 赵雯慧, 付开赟, 丁新华, 贾尊尊, 阿地力·沙塔尔, 郭文超. 三种迷向装置对桃小食心虫的防治效果[J]. 新疆农业科学, 2023, 60(12): 3041-3048. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||