新疆农业科学 ›› 2024, Vol. 61 ›› Issue (1): 34-41.DOI: 10.6048/j.issn.1001-4330.2024.01.005
• 作物遗传育种·种质资源·分子遗传学·土壤肥料 • 上一篇 下一篇
成志慧1(), 李红梅1, 赵红梅1, 涂永峰2, 宋海英2, 盛建东1()
收稿日期:
2023-06-10
出版日期:
2024-01-20
发布日期:
2024-02-21
通信作者:
盛建东(1970-),男,甘肃秦安人,教授,博士,硕士生/博士生导师,研究方向为土壤质量空间变异、养分资源高效利用、土壤信息技术等,(E-mail)作者简介:
成志慧(1998-),女,新疆乌鲁木齐人,硕士研究生,研究方向为植物营养学,(E-mail)1911488163@qq.com
基金资助:
CHENG Zhihui1(), LI Hongmei1, ZHAO Hongmei1, TU Yongfeng2, SONG Haiying2, SHENG Jiandong1()
Received:
2023-06-10
Online:
2024-01-20
Published:
2024-02-21
Correspondence author:
SHENG Jiandong(1970-),male,native place Qinan,Gansu,Professor,research field:spatial variation ofsoil quality,efficient utilization of nutrient resources,soil information technology,(E-mail)Supported by:
摘要:
【目的】研究氮肥减施对棉花氮素吸收、转运及利用效率的影响,为绿洲滴灌棉田氮肥合理施用提供参考依据。【方法】利用15N同位素示踪技术,采用盆栽试验,设置不施氮肥(N0)、氮肥减施(N1,225 kg/hm2)、当地推荐施肥(N2,300 kg/hm2)3个处理,研究氮肥减施对棉花生物量、氮素吸收量、肥料氮吸收量以及氮肥利用率的影响。【结果】氮肥减施处理的棉花干物质积累量和氮素吸收量均显著高于不施氮处理(N0),而与当地推荐施肥处理差异均不显著;氮肥减施处理棉花壳、籽的干物质量和籽的氮素吸收量均显著高于当地推荐施肥处理,分别增加了7.02%、6.81%和16.59%;2个施肥处理棉籽的肥料氮(15N)积累量占比最高,占全株的26.02%~35.99%,其次为叶(22.40%~23.81%)和茎(14.48%~18.98%),絮中最少(3.05%~6.62%);氮肥减施处理生殖器官的肥料氮吸收量比当地推荐施肥处理高19.02%;2个施肥处理的棉株吸收的氮素来源于肥料氮的比例(Ndff%)为21.14%~21.70%;氮肥减施处理的氮肥利用率和表观利用率均显著高于当地推荐施肥处理,分别增加2.36%和3.12%。【结论】氮肥减施(225 kg/hm2)可以显著提高棉籽中的干物质量、氮素吸收量、肥料氮(15N)吸收量、Ndff%以及棉花整株的氮肥利用率,在确保棉花产量不降低的前提下,适度的减少化肥施用量是提高化肥利用率最有效的措施之一。
中图分类号:
成志慧, 李红梅, 赵红梅, 涂永峰, 宋海英, 盛建东. 15N同位素标记氮肥减施棉田养分利用效率分析[J]. 新疆农业科学, 2024, 61(1): 34-41.
CHENG Zhihui, LI Hongmei, ZHAO Hongmei, TU Yongfeng, SONG Haiying, SHENG Jiandong. Analysis of nutrient use efficiency in cotton fields with 15N isotope labeled nitrogen fertilizer[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 34-41.
图1 不同处理下棉花干物质积累量和氮素总吸收量变化 注:不同小写字母表示不同处理间差异显著(P<0.05)
Fig.1 Dry matter accumulation and total nitrogen uptake of cotton Note:Different lowercase letters indicate significant differences between treatments(P<0.05)
图2 不同氮肥处理下棉花各器官干物质量和氮素吸收量变化 注:不同小写字母表示不同处理间差异显著(P<0.05)
Fig.2 Dry matter quality and nitrogen uptake of cotton organs Note:Different lowercase letters indicate significant differences between treatments(P<0.05)
器官 Organs | 处理 Treatments | 肥料氮 吸收量 Fertilizer nitrogen uptake (mg/株) | 肥料氮 分配率 Fertilizer nitrogen allocation rate(%) |
---|---|---|---|
根Root | N1 | 3.96±0.68 a | 9.30±1.35 a |
N2 | 4.08±0.32 a | 10.13±1.15 a | |
茎Stem | N1 | 6.11±0.17 a | 14.48±0.72 a |
N2 | 7.78±1.00 a | 18.98±1.73 a | |
叶Leaf | N1 | 9.47±0.27 a | 22.40±0.86 a |
N2 | 9.69±0.67 a | 23.81±0.92 a | |
絮Fibre | N1 | 1.29±0.06 c | 3.05±0.18 b |
N2 | 2.66±0.39 a | 6.62±1.23 a | |
壳Shell | N1 | 6.27±0.67 a | 14.78±1.36 a |
N2 | 5.89±0.59 a | 14.44±1.15 a | |
籽Seed | N1 | 15.22±0.26 a | 35.99±0.60 a |
N2 | 10.60±0.83 b | 26.02±1.13 b | |
整株 Whole plant | N1 | 42.33±1.12 a | - |
N2 | 40.68±1.10 a | - |
表1 棉花不同器官吸收与分配肥料氮(15N)变化
Tab.1 Uptake and distribution of nitrogen(15N) in different organs of cotton
器官 Organs | 处理 Treatments | 肥料氮 吸收量 Fertilizer nitrogen uptake (mg/株) | 肥料氮 分配率 Fertilizer nitrogen allocation rate(%) |
---|---|---|---|
根Root | N1 | 3.96±0.68 a | 9.30±1.35 a |
N2 | 4.08±0.32 a | 10.13±1.15 a | |
茎Stem | N1 | 6.11±0.17 a | 14.48±0.72 a |
N2 | 7.78±1.00 a | 18.98±1.73 a | |
叶Leaf | N1 | 9.47±0.27 a | 22.40±0.86 a |
N2 | 9.69±0.67 a | 23.81±0.92 a | |
絮Fibre | N1 | 1.29±0.06 c | 3.05±0.18 b |
N2 | 2.66±0.39 a | 6.62±1.23 a | |
壳Shell | N1 | 6.27±0.67 a | 14.78±1.36 a |
N2 | 5.89±0.59 a | 14.44±1.15 a | |
籽Seed | N1 | 15.22±0.26 a | 35.99±0.60 a |
N2 | 10.60±0.83 b | 26.02±1.13 b | |
整株 Whole plant | N1 | 42.33±1.12 a | - |
N2 | 40.68±1.10 a | - |
图3 棉花不同器官吸收土壤氮和肥料氮变化 注:Ndff%表示植株各器官的氮素来源于肥料氮的比例; Ndfs%表示植株各器官的氮素来源于土壤氮的比例; 不同小写字母表示同一氮素来源不同处理间差异显著(P<0.05)
Fig.3 Uptake of soil nitrogen and fertilizer nitrogen by different organs of cotton Note:Ndff% indicates the proportion of nitrogen in each plant organ that comes from fertilizer nitrogen. Ndfs% indicates the proportion of nitrogen in each plant organ that comes from soil nitrogen. Different lowercase letters indicated significant difference among different treatments of the same nitrogen source(P<0.05).
图4 棉花氮肥利用率和表观利用率变化 注:N1和N2对应的施氮量为500和667 mg/株; 不同小写字母表示不同处理间差异显著(P<0.05)
Fig.4 Cotton nitrogen use efficiency and apparent utilization rate Note:15N rates in N1 and N2 are 500、667 mg/plant. Different lowercase letters indicate significant differences between treatments(P<0.05)
[1] |
石洪亮, 严青青, 张巨松, 等. 氮肥对非充分灌溉下棉花花铃期光合特性及产量的补偿作用[J]. 作物学报, 2018, 44(8):1196-1204.
DOI |
SHI Hongliang, YAN Qingqing, ZHANG Jusong, et al. Effects of different nitrogen fertilizer levels on growth,yield,water and nitrogen use efficiency of cotton under non-sufficient drip irrigation[J]. Acta Agronomica Sinica, 2018, 44(8):1196-1204.
DOI |
|
[2] |
Matson P A, Naylor R. Ortiz-Monasterio I. Integration of environmental,agronomic,and economic aspects of fertilizer management[J]. Science, 1998, 280(5360):112-115.
PMID |
[3] |
Galloway J N, Townsend A R, Erisman J W, et al. Transformation of the nitrogen cycle:recent trends,questions,and potential solutions[J]. Science, 2008, 320(5878):889-892.
DOI PMID |
[4] | Liu J G, You L Z, Amini M, et al. A high-resolution assessment on global nitrogen flows in cropland[C]. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(17):8035-8040. |
[5] |
Bedard-Haughn A, van Groenigen J W, van Kessel C. Tracing 15N through landscapes:potential uses and precautions[J]. Journal of Hydrology, 2003, 272(1-4):175-190.
DOI URL |
[6] |
Šturm M, Kacjan-Maršiĉ N, Zupanc V, et al. Effect of different fertilization and irrigation practices on yield,nitrogen uptake and fertiliser use efficiency of white cabbage(Brassica oleracea var. capitata L.)[J]. Scientia Horticulturae, 2010, 125(2):103-109.
DOI URL |
[7] | 王士红, 聂军军, 李秋芝, 等. 施氮量对土壤-棉花系统中氮素吸收利用和氮素去向的影响[J]. 植物营养与肥料学报, 2020, 26(4):738-745. |
WANG Shihong, NIE Junjun, LI Qiuzhi, et al. Effects of nitrogen application rate on nitrogen absorption and utilization,and fate of nitrogen in soil-cotton system[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(4):738-745. | |
[8] | 侯振安, 李品芳, 龚江, 等. 不同滴灌施肥策略对棉花氮素吸收和氮肥利用率的影响[J]. 土壤学报, 2007, 44(4):702-708. |
HOU Zhenan, LI Pengfang, GONG Jiang, et al. Effects of fertigation strategy on nitrogen uptake by cotton and use efficiency of N fertilizer[J]. Acta Pedologica Sinica, 2007, 44(4):702-708. | |
[9] | 李鹏程, 董合林, 刘爱忠, 等. 应用15N研究氮肥运筹对棉花氮素吸收利用及产量的影响[J]. 植物营养与肥料学报, 2015, 21(3):590-599. |
LI Pengcheng, DONG Helin, LIU Aizhong, et al. Effects of nitrogen fertilizer application strategy on N uptake,utilization and yield of cotton using 15N trace technique[J]. Journal of Plant Nutrition and Fertilizers, 2015, 21(3):590-599. | |
[10] | 李鹏程, 郑苍松, 孙淼, 等. 利用15N示踪研究不同肥力土壤棉花氮肥减施的产量与环境效应[J]. 植物营养与肥料学报, 2017, 23(5):1199-1206. |
LI Pengcheng, ZHENG Cangson, SUN Miao, et al. Using 15N tracing technique to study the yield and environmental effect of decreasing N fertilization on cotton in different fertility fields[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(5):1199-1206. | |
[11] | 马丽娟, 侯振安, 闵伟, 等. 适宜咸水滴灌提高棉花水氮利用率[J]. 农业工程学报, 2013, 29(14):130-138. |
MA Lijuan, HOU Zhenan, MIN Wei, et al. Drip irrigation with suitable saline water improves water use efficiency for cotton[J]. Transactions of the Chinese Society of Agricultural Engineering, 2013, 29(14):130-138. | |
[12] | 张文, 周广威, 闵伟, 等. 应用15N示踪法研究咸水滴灌棉田氮肥去向[J]. 土壤学报, 2015, 52(2):372-380. |
ZHANG Wen, ZHOU Guangwei, MIN wei, et al. Fate of fertilizer N in saline water drip-irrigated cotton field using 15N tracing method[J]. Acta Pedologica Sinica, 2015, 52(2):372-380. | |
[13] |
张宏, 曾雄, 王爱莲, 等. 不同施氮量对棉花产量、养分吸收及氮素利用的影响[J]. 新疆农业科学, 2021, 58(9):1656-1664.
DOI |
ZHANG Hong, CENG Xiong, WANG Ailiang, et al. Effects of different nitrogen application rates on yield,nutrient uptake and nitrogen utilization of cotton in southern Xinjiang[J]. Xinjiang Agricultural Sciences, 2021, 58(9):1656-1664.
DOI |
|
[14] | 王海东, 张富仓, 吴立峰, 等. 滴灌施肥量对棉花生长、养分吸收及产量的影响[J]. 干旱地区农业研究, 2015, 33(4):98-104,250. |
WWANG Haidong, ZHANG Fucang, WU Lifeng, et al. Dosage effects of dripping fertilization on cotton growth,nutrient uptake and yield[J]. Agricultural Research in the Arid Areas, 2015, 33(4):98-104,250. | |
[15] |
李春梅, 马云珍, 徐文修, 等. 不同施氮量对棉花产量和棉田土壤养分的影响[J]. 核农学报, 2022, 36(7):1446-1455.
DOI |
LI Chunmei, MA Yunzhen, XU Wenxiu, et al. Effects of different nitrogen application rates on cotton yield and soil nutrients in cotton fields.[J]. Journal of Nuclear Agricultural Sciences, 2022, 36(7):1446-1455.
DOI |
|
[16] | 全国土壤普查办公室. 中国土壤普查技术[M]. 北京: 农业出版社,1992. |
National Soil Survey Office. China Soil Survey Technology[M]. Beijing: Agriculture Press,1992. | |
[17] |
郭小琰, 孙桂兰, 熊世武, 等. 施氮量对棉花养分吸收利用及产量和品质的影响[J]. 新疆农业科学, 2021, 58(7):1246-1254.
DOI |
GUO Xiaoyan, SUN Guilan, XIONG Shiwu, et al. Effects of nitrogen application rates on nutrition uptake and utilization,yield and fiber quality of cotton[J]. Xinjiang Agricultural Sciences, 2021, 58(7):1246-1254.
DOI |
|
[18] |
李飞, 郭莉莉, 赵瑞元, 等. 氮肥减施深施对油后直播棉花干物质与氮素积累、分配及产量的影响[J]. 棉花学报, 2022, 34(3):198-214.
DOI |
LI Fei, GUO Lili, ZHAO Ruiyuan, et al. Effects of increasing application depth and decreasing nitrogen rate on dry matter,nitrogen accumulation and distribution,and yield of direct seeding cotton after rape harvest[J]. Cotton Science, 2022, 34(3):198-214. | |
[19] | 马宗斌, 严根土, 刘桂珍, 等. 施氮量对黄河滩区棉花叶片生理特性、干物质积累及产量的影响[J]. 植物营养与肥料学报, 2013, 19(4):849-857. |
MA Zongbin, YAN Gentu, LIU Guizhen, et al. Effects of nitrogen application rates on main physiological characteristics of leaves,dry matter accumulation and yield of cotton cultivated in the Yellow River bottomlands[J]. Journal of Plant Nutrition and Fertilizer, 2013, 19(4):849-857. | |
[20] | 石洪亮, 张巨松, 严青青, 等. 非充分滴灌下施氮量对棉花生长特性、产量及水氮利用率的影响[J]. 干旱地区农业研究, 2017, 35(4):129-136. |
SHI Hongliang, ZHANG Jusong, YAN Qingqing, et al.. Effects of different nitrogen fertilizer levels on growth,yield,water and nitrogen use efficiency of cotton under non-sufficient drip irrigation[J]. Agricultural Research in the Arid Areas, 2017, 35(4):129-136. | |
[21] | 邹芳刚, 郭文琦, 王友华, 等. 施氮量对长江流域滨海盐土棉花氮素吸收利用的影响[J]. 植物营养与肥料学报, 2015, 21(5):1150-1158. |
ZOU Fanggang, GUO Wenqi, WANG Youhua, et al. Effects of nitrogen application rate on the nitrogen uptake and utilization of cotton grown in coastal saline fields of Yangtze River Valley[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(5):1150-1158. | |
[22] | 周瑞庆, 陈开铁, 李合松, 等. 应用15N示踪技术研究水稻对氮素的吸收利用[J]. 湖南农学院学报, 1991, 17(4):665-669. |
ZHOU Ruiqing, CHEN Kaitie, LI Hesong, et al. Nitrogen absorption rate of rice plants with 15N labeling[J]. Journal of Hunan Agricultural College, 1991, 17(4):665-669. | |
[23] |
晏娟, 沈其荣, 尹斌, 等. 应用15N示踪技术研究水稻对氮肥的吸收和分配[J]. 核农学报, 2009, 23(3):487-491,496.
DOI |
YAN Juan, SHEN Qirong, YIN Bin, et al. Fertilizer-N uptake and distribution in rice plants using 15N tracer technique[J]. Journal of Nuclear Agricultural Sciences, 2009, 23(3):487-491,496.
DOI |
|
[24] |
戴良香, 张智猛, 张冠初, 等. 氮肥用量对花生氮素吸收与分配的影响[J]. 核农学报, 2020, 34(2):370-375.
DOI |
DAI Liangxiang, ZHANG Zhimeng, ZHANG Guanchu, et al. Effects of nitrogen application on nitrogen uptake and distribution in peanut[J]. Journal of Nuclear Agricultural Sciences, 2020, 34(2):370-375.
DOI |
|
[25] |
李灿东, 郭泰, 王志新, 等. 叶面不同施氮量对大豆氮素吸收与分配的影响[J]. 核农学报, 2015, 29(3):557-562.
DOI |
LI Chandong, GUO Tai, WANG Zhixin, et al. Effects of leavse nitrogen application rate on nitrogen uptake and distribution in soybean[J]. Journal of Nuclear Agricultural Sciences, 2015, 29(3):557-562.
DOI |
|
[26] | 徐聪, 李欢, 史衍玺. 不同施氮量对甘薯氮素吸收与分配的影响[J]. 水土保持学报, 2014, 28(2):149-153. |
XU Cong, LI Huan, SHI Yanxi. The effects of different nitrogen levels on nitrogen uptake and distribution of sweet potato[J]. Journal of Soil and Water Conservation, 2014, 28(2):149-153. | |
[27] | 王娟, 马腾飞, 危常州, 等. 不同灌溉方式对棉花氮素吸收利用和氮肥利用率的影响[J]. 石河子大学学报(自然科学版), 2011, 29(6):670-673. |
WANG Juan, MA Tengfei, WEI Changzhou, et al. Effect of different irrigation patterns on cotton nitrogen absorption and nitrogen fertilizer use efficiency[J]. Journal of Shihezi University (Natural Science Ed.), 2011, 29(6):670-673. | |
[28] | 朱兆良. 中国土壤氮素研究[J]. 土壤学报, 2008, 45(5):778-783. |
ZHU Zhaoliang. Research on soil nitrogen in China[J]. Acta Pedologica Sinica, 2008, 45(5):778-783. | |
[29] | 潘晓丽, 林治安, 袁亮, 等. 不同土壤肥力水平下玉米氮素吸收和利用的研究[J]. 中国土壤与肥料, 2013,(1):8-13. |
PAN Xiaoli, LIN Zhian, YUAN Liang, et al. Nitrogen uptake and use of summer maize under different soil fertility levels[J]. Soil and Fertilizer Sciences in China, 2013,(1):8-13. |
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