458份小麦品种(系)抗病性状功能基因的KASP标记检测

doi:10.6048/j.issn.1001-4330.2022.04.001

新疆农业科学 ›› 2022, Vol. 59 ›› Issue (4): 781-786.DOI: 10.6048/j.issn.1001-4330.2022.04.001

• 作物遗传育种·分子遗传学·耕作栽培·种质资源 • 上一篇下一篇

458份小麦品种(系)抗病性状功能基因的KASP标记检测

王重¹^,²(), 张宏芝¹^,², 高新¹^,³, 时佳¹^,³, 王立红¹^,², 李剑峰¹^,³, 樊哲儒¹^,², 赵奇¹^,², 张跃强¹^,²()

1.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091
2.农业部荒漠绿洲作物生理生态与耕作重点实验室, 乌鲁木齐 830091
3.新疆农作物生物技术重点实验室,乌鲁木齐 830091

收稿日期:2022-02-21 出版日期:2022-04-20 发布日期:2022-04-24
通信作者: 张跃强
作者简介:王重(1982-),男,陕西长安人,副研究员,研究方向为小麦遗传育种,(E-mail) wangzhongac@126.com
基金资助:
新疆维吾尔自治区科技支疆项目(2019E0250)

KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars (Lines)

WANG Zhong¹^,²(), ZHANG Hongzhi¹^,², GAO Xin¹^,³, SHI Jia¹^,³, WANG Lihong¹^,², LI Jianfeng¹^,³, FAN Zheru¹^,², ZHAO Qi¹^,², ZHANG Yueqiang¹^,²()

1. Research Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
2. Key Laboratory of Desert-Oasis Crop Physiological Ecology and Cultivation, Ministry of Agriculture and Rural Affairs of the P.R.C., Urumqi 830091, China
3. Key Laboratory of Xinjiang Crop Biotechnology, Urumqi 830091, China

Received:2022-02-21 Online:2022-04-20 Published:2022-04-24
Correspondence author: ZHANG Yueqiang
Supported by:
Science and Technology Supporting Xinjiang Project of Xinjiang Uygur Autonomous Region(2019E0250)

摘要/Abstract

摘要：

【目的】研究不同小麦品种(系)抗病性状功能基因的分布状态,分析小麦品种(系)的抗病功能基因,为小麦抗病育种提供理论依据。【方法】利用KASP技术通过1个抗白粉病分子标记(Pm21)、1个抗条锈病分子标记(Yr15)和2个抗叶锈病分子标记(Lr14、Lr68),检测458份小麦品种(系)。【结果】筛选出携带Pm21标记材料2份,Yr15标记材料14份,Lr14a标记材料22份,Lr68标记材料48份。【结论】新疆小麦品种(系)中抗病基因的分布频率较低,约为5%。筛选出的86份材料可以作为新疆小麦抗病育种的亲本材料加以应用。

关键词: 小麦; 抗病基因; KASP; 标记检测

Abstract:

【Objective】 This project aims to study the distribution of functional genes for disease resistance of different wheat varieties (lines) and clarify the in the hope of providing theoretical basis for wheat disease resistance breeding. 【Method】 458 wheat varieties (lines) were detected by KASP through one powdery mildew resistance molecular marker (Pm21), one stripe rust resistance molecular marker (Yr15) and two leaf rust resistance molecular markers (Lr14a and Lr68). 【Result】 There were 2 Pm21 carried lines, 14 Yr15 carried lines, 22 Lr14a carried lines and 48 Lr68 carried lines in 458 wheat cultivars (lines). 【Conclusion】 The distribution frequency of resistance genes in Xinjiang wheat varieties (lines) was about 5% and the 86 lines selected can be used as the parents for resistance breeding of wheat in Xinjiang.

Key words: wheat; disease resistance gene; KASP; marker assays

中图分类号:

S512

王重, 张宏芝, 高新, 时佳, 王立红, 李剑峰, 樊哲儒, 赵奇, 张跃强. 458份小麦品种(系)抗病性状功能基因的KASP标记检测[J]. 新疆农业科学, 2022, 59(4): 781-786.

WANG Zhong, ZHANG Hongzhi, GAO Xin, SHI Jia, WANG Lihong, LI Jianfeng, FAN Zheru, ZHAO Qi, ZHANG Yueqiang. KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars (Lines)[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 781-786.

图/表 5

图1 白粉病抗性标记Pm21检测注:红色:无Pm21;蓝色:携带Pm21

Fig.1 Detection of powdery mildew resistance marker Pm21 Note:Red: No Pm21; Blue: Contains Pm21

图2 条锈病抗性标记Yr15检测注:红色:携带Yr15;蓝色:无Yr15

Fig.2 Detection of stripe rust resistance markerYr15 Note:Red: Contains Yr15; Blue: No Yr15

图3 叶锈病抗性标记Lr14a检测注:红色:携带Lr14a;蓝色:无Lr14a

Fig.3 Detection of leaf rust resistance marker Lr14a Note:Red: Contains Lr14a; Blue: No Lr14a

图4 叶锈病抗性标记Lr68检测注:红色:携带Lr68;蓝色:无Lr68

Fig.4 Detection of leaf rust resistance marker Lr68 Note:Red: Contains Lr68; Blue: No Lr68

表1 小麦抗病功能基因分布状态

Table 1 Distribution of disease-resistance genes in wheat

抗病功能基因 Disease resistance gene	不同小麦类型 Different wheat types		不同来源 Different sources			合计 Total
抗病功能基因 Disease resistance gene	冬小麦 Winter wheat	春小麦 Spring wheat	审定品种 Varieties	高代品系 Lines	引进种质资源 Introduced variety	合计 Total
Pm21	2(0.44%)	0	2(0.44%)	0	0	2(0.44%)
Yr15	3(0.66%)	11(2.40%)	7(1.53%)	6(1.31%)	1(0.22%)	14(3.06%)
Lr14a	20(4.37%)	2(0.44%)	21(4.59%)	0	1(0.22%)	22(4.80%)
Lr68	2(0.44%)	46(10.04%)	4(0.87%)	15(3.28%)	29(6.33%)	48(10.48%)

参考文献 17

[1]	新疆维吾尔自治区统计局. 新疆统计年鉴[J]. 北京: 中国统计出版社, 2019.
	Statistics Bureau of Xinjiang Uygur Autonomous Region. Xinjiang Statistical Yearbook[J]. Beijing: China Statistics Press, 2019.
[2]	高海峰, 白微微, 陈利, 等. 新疆小麦条锈病和白粉病越夏调查[J]. 新疆农业科学, 2017, 54(9):1670-1678. DOI
	GAO Haifeng, BAI Weiwei, CHEN Li, et al. Preliminary Report on Over-summering of Stripe Rust and Wheat Powdery Mildew in Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(9):1670-1678. DOI
[3]	崔阳, 王晓云. 竞争性等位基因特异性PCR分型技术在植物抗病遗传育种中的应用[J]. 分子植物育种, 2021, 19(1):164-172. DOI: 10.13271/j.mpb.019.000164. DOI
	CUI Yang, WANG Xiaoyun. Application of Kompetitive Allele-specific PCR Genotyping Technology in Plant Genetic Breeding for Disease Resistance[J]. Molecular Plant Breeding, 2021, 19(1):164-172. DOI: 10.13271/j.mpb.019.000164. DOI
[4]	李玮, 宋国琦, 商航, 等. 94份小麦育种亲本的功能基因分子检测[J]. 山东农业科学, 2021, 53(5):58-63.DOI: 10.14083/j.issn.1001-4942.2021.05.009. DOI
	LI Wei, SONG Guoqi, SHANG Hang, et al. Molecular Detection of Functional Genes in 94 Wheat Parent Lines[J]. Shandong Agricultural Sciences, 2021, 53(5):58-63. DOI: 10.14083/j.issn.1001-4942.2021.05.009. DOI
[5]	邹景伟, 贾万利, 李立鑫, 等. 120份小麦品种(系)重要性状功能基因的KASP检测[J]. 分子植物育种, 2019, 17(12):3945-3959.
	ZOU Jingwei, JIA Wanli, LI Lixin, et al. KASP Marker Assays for Functional Genes of Important Trait in 120 Wheat Cultivars (Lines)[J]. Molecular Plant Breeding, 2019, 17(12):3945-3959.
[6]	高洁, 宋国琦, 李吉虎, 等. 小麦4个多效抗病基因分子标记的转化和再开发[J]. 农业生物技术学报, 2021, 29(5):847-856.
	GAO Jie, SONG Guoqi, LI Jihu, et al. Convertion and Redevelopment of Molecular Markers of 4 Pleiotropic Disease Resistance Genes in Wheat (Triticum aestivum)[J]. Journal of Agricultural Biotechnology, 2021, 29(5):847-856.
[7]	潘娜, 郭会君, 赵世荣, 等. 小麦TILLING分析中CELⅠ酶切及PCR反应体系的优化[J]. 植物遗传资源学报, 2012, 13(5):830-837.
	PAN Na, GUO Huijun, ZHAO Shirong, et al. Establishment and Optimization of CEL I-based TILLING for Wheat[J]. Journal of Plant Genetic Resources, 2012, 13(5):830-837.
[8]	MA Jian, DING Puyang, LIU Jiajun, et al. Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat[J]. Theoretical and Applied Genetics, 2019, 132(5):3155-3167. DOI URL
[9]	HE Huagang, ZHU Shanying, ZHAO Renhui, et al. Pm21, encoding a typical CC-NBS-LRR protein, confers broad-spectrum resistance to wheat powdery mildew disease[J]. Molecular plant, 2018, 11(6):879-882. DOI PMID
[10]	XING Liping, HU Ping, LIU Jiaqian, et al. Pm21 from Haynaldia villosa Encodes a CC-NBS- LRR Protein Conferring Powdery Mildew Resistance in Wheat[J]. Molecular plant, 2018, 11(6):874-878. DOI PMID
[11]	王振花, 刘伟, 高海峰, 等. 新疆小麦白粉病菌群体的毒性监测和分析[J]. 新疆农业科学, 2017, 54(10):1903-1910. DOI
	WANG Zhenhua, LIU Wei, GAO Haifeng, et al. Monitoring and Analysis of Virulence of Blumeria graminis f. sp. tritici in Xinjiang[J]. Xinjiang Agricultural Sciences, 2017, 54(10):1903-1910. DOI
[12]	王鑫, 宋鹏博, 王笑笑, 等. 305份国内外小麦种质条锈病与白粉病抗性鉴定与评价[J]. 麦类作物学报, 2021, 41(6):689-698.
	WANG Xin, SONG Pengbo, WANG Xiaoxiao, et al. Identification and Evaluation of Resistance to Stripe Rust and Powdery Mildew of 305 Domestic and Foreign Wheat Germplasms[J]. Journal of Triticeae Crops, 2021, 41(6):689-698.
[13]	韩德俊, 康振生. 中国小麦品种抗条锈病现状及存在问题与对策[J]. 植物保护, 2018, 44(5):1-12.
	HAN Dejun, KANG Zhensheng. Current status and future strategy in breeding wheat for resistance to stripe rust in China[J]. Plant Protection, 2018, 44(5):1-12.
[14]	刘鹏, 陈璨, 卢家玲, 等. 小麦品种云麦53抗条锈基因的分子标记定位[J]. 麦类作物学报, 2015, 35(11):1483-1488.
	LIU Peng, CHEN Can, LU Jialing, et al. Molecular Mapping of Stripe Rust Resistance Gene in Wheat Cultivar Yunmai 53[J]. Journal of Triticeae Crops, 2015, 35(11):1483-1488.
[15]	Park R F, Mohler V, Nazari K, et al. Characterization and mapping of gene Lr73 conferring seedling resistance to Puccinia triticina in common wheat[J]. Theoretical and Applied Genetics, 2014, 127(9):2041-2049. DOI PMID
[16]	LI Zaifeng, LAN Caixia, HE Zhonghu, et al. Overview and application of QTL for adult plant resistance to leaf rust and powdery mildew in wheat[J]. Crop Science, 2014, 54(5):1907-1925. DOI URL
[17]	Herrera-Foessel S A, Singh R P, Huerta-Espino J, et al. Lr68: a new gene conferring slow rusting resistance to leaf rust in wheat[J]. Theoretical & Applied Genetics, 2012, 124(8):1475-1486.

458份小麦品种(系)抗病性状功能基因的KASP标记检测

KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars (Lines)

在线阅读

PDF下载

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 17

相关文章 15

编辑推荐

Metrics

[1]	陈传信, 张永强, 聂石辉, 孔德鹏, 赛力汗·赛, 徐其江, 雷钧杰. 生物质炭施用量对滴灌冬小麦生长发育和产量的影响[J]. 新疆农业科学, 2023, 60(9): 2146-2151.
[2]	王立红, 张宏芝, 张跃强, 李剑峰, 王重, 高新, 时佳, 王春生, 夏建强, 樊哲儒. 不同产量水平冬小麦产量差异形成的干物质生产、转运及氮肥利用分析[J]. 新疆农业科学, 2023, 60(9): 2152-2162.
[3]	王晓雨, 王小平, 史文宇, 刘美艳, 马健, 郭云鹏, 宋瑞欣, 王清涛. 拔节期冬小麦光合特性、干物质积累和产量对干旱胁迫的响应[J]. 新疆农业科学, 2023, 60(9): 2163-2172.
[4]	杨红梅, 张跃强, 史应武, 吾买尔江·库尔班, 林青, 王宁, 楚敏, 曾军. 不同类型叶面肥喷施对冬小麦籽粒产量和品质的影响[J]. 新疆农业科学, 2023, 60(9): 2182-2188.
[5]	秦鹏亮, 周霄, 王爽, 李佳琪, 刘颖, 张娜, 杨文香. 小麦品种衡观35抗茎基腐病EMS突变及其鉴定[J]. 新疆农业科学, 2023, 60(9): 2231-2238.
[6]	张立国, 郭春贵, 常立春, 郭新磊, 张涛, 武剑, 梁建丽, 高杰, 王晓武. 白菜抗根肿病分子标记开发与种质资源鉴定[J]. 新疆农业科学, 2023, 60(9): 2281-2290.
[7]	董艳雪, 贾永红, 张金汕, 李丹丹, 王凯, 罗四维, 王润琪, 石书兵. 不同生态区环境下春小麦干物质积累及产量形成分析[J]. 新疆农业科学, 2023, 60(8): 1848-1857.
[8]	冯梅, 刘超勤, 陈杰, 刘文龙, 杨志刚, 田杰英, 黄鑫. 小麦不同杂交F₁代性状变异及杂种优势分析[J]. 新疆农业科学, 2023, 60(8): 1858-1865.
[9]	李怀胜, 艾洪玉, 孟玲, 王贺亚, 张磊, 艾海峰. 减氮下运筹养分吸收高峰期追施比例对春小麦的影响[J]. 新疆农业科学, 2023, 60(8): 1866-1872.
[10]	张永强, 陈传信, 聂石辉, 徐其江, 赛力汗·赛, 雷钧杰. 矮壮素滴施时期对冬小麦茎秆抗倒伏能力的调控分析[J]. 新疆农业科学, 2023, 60(8): 1873-1878.
[11]	杨金钰, 王西和, 孙九胜. 水培对全株大麦和小麦苗氨基酸组成的影响及对比分析[J]. 新疆农业科学, 2023, 60(7): 1589-1595.
[12]	田文强, 董艳雪, 史永清, 雷钧杰, 孙刚刚, 王泓懿, 聂凌帆, 郭飞, 艾洪玉, 石书兵, 张金汕. 播期对超晚冬麦茎秆性状及群体动态的影响[J]. 新疆农业科学, 2023, 60(6): 1301-1307.
[13]	董德誉, 程宇坤, 王睿, 雷钧杰, 王伟, 陈传信, 张永强, 耿洪伟. 基于无人机多光谱影像反演不同生育期小麦光合参数分析[J]. 新疆农业科学, 2023, 60(6): 1308-1318.
[14]	张永强, 陈传信, 徐其江, 聂石辉, 雷钧杰, 刘昌文. 氮肥增效剂与氮肥减量配施对冬小麦叶片生理及产量的影响[J]. 新疆农业科学, 2023, 60(6): 1319-1325.
[15]	赖宁, 耿庆龙, 李永福, 李娜, 信会男, 步生兵, 陈署晃. 有机无机配施对超晚播冬小麦产量、氮磷养分吸收利用及土壤肥力的影响[J]. 新疆农业科学, 2023, 60(6): 1335-1343.