小麦品种衡观35抗茎基腐病EMS突变及其鉴定

doi:10.6048/j.issn.1001-4330.2023.09.018

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (9): 2231-2238.DOI: 10.6048/j.issn.1001-4330.2023.09.018

小麦品种衡观35抗茎基腐病EMS突变及其鉴定

秦鹏亮¹(), 周霄², 王爽¹, 李佳琪¹, 刘颖¹, 张娜¹, 杨文香¹()

1.河北农业大学植物保护学院植物病理学系/河北省农作物病虫害生物防治技术创新中心/国家北方山区农业工程技术研究中心,河北保定 071001
2.河北省植保植检总站,石家庄 050000

收稿日期:2022-12-28 出版日期:2023-09-20 发布日期:2023-09-19
通信作者: 杨文香(1966-),女,河北保定人,教授,博士,硕士生/博士生导师,研究方向为小麦病害防控,(E-mail)wenxiangyang2003@163.com
作者简介:秦鹏亮(1994-),男,陕西宝鸡人,硕士研究生,研究方向为小麦叶锈菌致病机制,(E-mail)1120469245@qq.com
基金资助:
国家重点研发计划项目(2017YFD020170704);河北省产业体系小麦创新团队(HCT2018010204);河北省重点研发计划项目(19226507D)

EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot

QIN Pengliang¹(), ZHOU Xiao², Kahsay Tadesse Mawcha¹, WANG Shuang¹, LI Jiaqi¹, LIU Ying¹, ZHANG Na¹, YANG Wenxiang¹()

1. Technological Innovation Centre for Biological Control of Crop Diseases and Insect Pests of Hebei Province / College of Plant Protection,Hebei Agricultural University, Baoding Hebei 071001, China
2. Hebei Plant Protection and Quarantine General Station,Shijiazhuang 050000, China

Received:2022-12-28 Online:2023-09-20 Published:2023-09-19
Correspondence author: YANG Wenxiang (1966-), female,native place; Baoding, Hebei, Ph.D., professor, doctoral surpervisor research field; the prevention and control of major wheat diseases, (E-mail)wenxiangyang2003@163.com
Supported by:
National key research and development project(2017YFD020170704);Hebei Province industrial system wheat innovation team(HCT2018010204);Key research and development project of Hebei(19226507D)

摘要/Abstract

摘要：

【目的】研究小麦品种衡观35抗茎基腐病EMS突变及其鉴定。【方法】利用不同浓度的甲基磺酸乙酯(EMS),对小麦品种衡观35进行诱变,以未经诱变的衡观35为感病对照,以石优17为中度抗病对照。人工接种假禾谷镰刀菌,对诱变材料进行抗性鉴定,经温室加代获得抗茎基腐病M₃代材料。【结果】适于衡观35突变的EMS浓度为0.4%,并且从106株M₃衡观35材料中获得7株期表现免疫的突变株,突变体材料苗期和成株期整体病情指数比感病对照分别降低34.93%和34.62%。【结论】EMS突变小麦衡观35,适宜的突变浓度为0.4%,共从供试突变品种M₃代中筛选获得了7份免疫的抗小麦茎基腐病的资源。

关键词: 小麦茎基腐病; EMS; 诱变; 突变株; 抗性鉴定

Abstract:

【Objective】 Study EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot.【Methods】 In this study, different concentrations of ethyl methanesul fonate (EMS) were employed on wheat seeds of cultivar Hengguan 35.The untreated Hengguan 35 was used as the susceptible control, and Shiyou 17 was used as the moderately resistant control.Through artificial inoculation of Fusarium pseudograminearum, the resistance of the mutagenized material was identified, and the M₃ generation material resistant to FCR was obtained by adding generation in the greenhouse.【Results】 The EMS concentration suitable for Hengguan 35 mutation was finally determined to be 0.4%, and seven mutants showing immunity at the plant stage were obtained from 106 M₃ Hengguan 35 materials.The overall disease index of the mutant materials at seedling and adult stages was 34.93% and 34.62% lower than those of the susceptible control, respectively.【Conclusion】 After EMS mutation of wheat Hengguan 35, an appropriate mutation concentration of 0.4% was determined. A total of 7 immune resistant wheat FCR were screened from the M3 generation of the tested mutant varieties.

Key words: Fusarium crown rot (FCR); EMS; mutagenesis; mutant; resistance identification

中图分类号:

S512
S188

秦鹏亮, 周霄, 王爽, 李佳琪, 刘颖, 张娜, 杨文香. 小麦品种衡观35抗茎基腐病EMS突变及其鉴定[J]. 新疆农业科学, 2023, 60(9): 2231-2238.

QIN Pengliang, ZHOU Xiao, Kahsay Tadesse Mawcha, WANG Shuang, LI Jiaqi, LIU Ying, ZHANG Na, YANG Wenxiang. EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot[J]. Xinjiang Agricultural Sciences, 2023, 60(9): 2231-2238.

图/表 7

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品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	26	4.76	127	23.26	111	20.33	102	18.68	94	17.22	86	15.75	546	53.52^a
M₀衡观35 M₀ Hengguan 35	106	14.50	148	20.25	182	24.90	137	18.74	77	10.53	81	11.08	731	44.76^b
石优17Shiyou 17	11	4.01	43	15.69	67	24.45	64	23.36	57	20.80	32	11.68	274	55.26^a

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	26	4.76	127	23.26	111	20.33	102	18.68	94	17.22	86	15.75	546	53.52^a
M₀衡观35 M₀ Hengguan 35	106	14.50	148	20.25	182	24.90	137	18.74	77	10.53	81	11.08	731	44.76^b
石优17Shiyou 17	11	4.01	43	15.69	67	24.45	64	23.36	57	20.80	32	11.68	274	55.26^a

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	4	8.00	12	24.00	14	28.00	9	18.00	8	16.00	3	6.00	50	45.60^a
M₃衡观35 M₃ Hengguan 35	25	20.83	28	23.33	51	42.50	16	13.33	0	0.00	0	0.00	120	29.67^b
石优17Shiyou 17	3	6.98	25	58.14	7	16.28	6	13.95	2	4.65	0	0.00	43	30.23^b

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	4	8.00	12	24.00	14	28.00	9	18.00	8	16.00	3	6.00	50	45.60^a
M₃衡观35 M₃ Hengguan 35	25	20.83	28	23.33	51	42.50	16	13.33	0	0.00	0	0.00	120	29.67^b
石优17Shiyou 17	3	6.98	25	58.14	7	16.28	6	13.95	2	4.65	0	0.00	43	30.23^b

品种 Variety	0级Grade 0		Ⅰ级Grade Ⅰ		Ⅱ级Grade Ⅱ		Ⅲ级Grade Ⅲ		Ⅳ级Grade Ⅳ		Ⅴ级Grade Ⅴ		总株数 Total number of plants	病情指数 Disease index
品种 Variety	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	株数 Number	占比 Proportion (%)	总株数 Total number of plants	病情指数 Disease index
衡观35Hengguan 35	0	0.00	12	24.00	14	28.00	10	20.00	10	20.00	4	8.00	50	52.00^a
M₃衡观35 M₃ Hengguan 35	7	5.83	40	30.00	55	45.83	18	15.00	0	0.00	0	0.00	120	34.00^b
石优17Shiyou 17	1	2.33	26	60.47	7	16.28	7	16.28	2	4.65	0	0.00	43	32.09^b

小麦品种衡观35抗茎基腐病EMS突变及其鉴定

EMS mutants of wheat hengguan 35 and identification of the mutants resistant to fusarium crown rot

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Metrics

品种 Variety	千粒重(g) 1000 grain weight (g)
品种 Variety	0级 Grade 0	Ⅰ级 Grade Ⅰ	Ⅱ级 Grade Ⅱ	Ⅲ级 Grade Ⅲ
衡观35 Hengguan 35	40.23^b	32.51^b	28.10^b	15.18^b
M₃衡观35 M₃ Hengguan 35	43.96^a	38.51^a	32.72^a	20.10^a
石优17 Shiyou 17	40.37^b	34.43^b	29.13^b	17.16^b

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