化学预处理与傅里叶变换红外光谱相结合分析棉花纤维细胞壁组分

doi:10.6048/j.issn.1001-4330.2022.02.001

摘要/Abstract

摘要：

【目的】 研究能够与傅里叶变换红外光谱(FTIR)相结合的分析棉花纤维细胞壁组分的化学预处理方法,使棉花纤维细胞壁断裂破碎但不造成其组分结构破坏,为实现应用FTIR分析细胞壁的真实结构组分提供参考。【方法】 采用化学试剂巯基乙酸和盐酸预处理棉花纤维,用去离子水清洗游离的化学试剂,再通过FTIR化学预处理后的棉花纤维细胞壁。利用木质化程度和硬度较高的樟子松枝条进行相同处理作为对照,观察分析。【结果】 采用化学试剂巯基乙酸和盐酸预处理棉花纤维,纤维在其与巯基乙酸中的巯基结合的部位被打碎,可以有效破碎棉花纤维细胞壁,克服了纤维样品难以磨碎的问题,再用去离子水清洗游离的化学试剂,通过FTIR观察化学预处理后的棉花纤维细胞壁,发现化学处理过的棉花纤维在1 800~1 650 cm^-1的波谱区间内显著高于原始切碎棉花纤维,在1 800~1 550 cm^-1的波谱区间内,化学处理棉花纤维和切碎纤维间存在极显著差异,在1 320~1 200 cm^-1波谱内,处理棉花纤维的FTIR光谱吸光度值显著高于切碎棉花纤维。【结论】 化学试剂巯基乙酸和盐酸预处理棉花纤维,再与FTIR相结合可以正确评价棉花纤维细胞壁组分。

关键词: 棉花; 细胞壁; 傅里叶变换红外光谱; 化学预处理

Abstract:

【Objective】 To provide a chemical pretreatment method which can be combined with fourier transform infrared spectroscopy (FTIR) to observe the cell wall components of cotton fiber.In the method, the cell wall of cotton fiber can be broken without causing structural damage and the real structural components of cell wall can be analyzed by FTIR. 【Method】 Cotton fiber was pretreated with thioglycolic acid and hydrochloric acid, and the free chemical reagent was washed with deionized water.The cell wall of cotton fiber after chemical pretreatment was observed by FTIR.Using the same treatment of the shoots of Pinus sylvestris L.var.mongolica Litv. with high lignification and hardness as control to verify the treatment method. 【Result】 Cotton fiber was broken at the site where it binds to the sulfhydryl group in thioglycolic acid, which could effectively break the cell wall of cotton fiber and overcome the problem that it was difficult to grind the fiber sample. Then the free chemical reagents were washed with deionized water. The cell wall of cotton fiber after chemical pretreatment was observed by FTIR. It was found that the chemically treated cotton fiber was significantly higher than the original chopped cotton fiber in the spectral range of 1,800-1,650 cm^-1. In the spectral range of 1,800-1,550 cm^-1, there was a very significant difference between chemically treated cotton fiber and chopped fiber, in the spectral range of 1,320-1,200 cm^-1, The FTIR absorbance of treated cotton fiber was significantly higher than that of chopped cotton fiber. 【Conclusion】 The cell wall components of cotton fiber can be correctly evaluated by pretreatment of cotton fiber with thioglycolic acid and hydrochloric acid and then combined with FTIR.

Key words: cotton fiber; cell wall; fourier transform infrared spectroscopy (FTIR); chemical pretreatment

中图分类号:

S562

胡文冉, 杨洋, 李波, 郝晓燕, 赵准, 邵武奎, 黄全生. 化学预处理与傅里叶变换红外光谱相结合分析棉花纤维细胞壁组分[J]. 新疆农业科学, 2022, 59(2): 261-268.

HU Wenran, YANG Yang, LI Bo, HAO Xiaoyan, ZHAO Zhun, SHAO Wukui, HUANG Quansheng. Analysis of Components of Cotton Fiber Cell Wall by Chemical Pretreatment and Fourier Transform Infrared Spectroscopy (FTIR)[J]. Xinjiang Agricultural Sciences, 2022, 59(2): 261-268.

图/表 6

图1 棉花纤维样品和樟子松样品的电镜扫描注:A.切碎的棉花纤维;B.巯基乙酸和盐酸处理并清洗过的棉花纤维;C.粉碎的樟子松枝条粉末;D.巯基乙酸和盐酸处理并清洗过的樟子松枝条粉末

Fig.1 FTIR spectrum of cotton fiber and Pinus sylvestris var.mongolica Litv. Note:A.SEM image of chopped cotton fiber; B.SEM image of cotton fiber treated by thioglycolic acid and HCl and washed by deionized water; C.SEM image of pulverized powder of Pinus sylvestris L.var.mongolica Litv.; D.SEM image of powder of Pinus sylvestris L.var.mongolica Litv.treated by thioglycolic acid and HCl and washed by deionized water

图2 棉花纤维样品的FTIR光谱注:Gh_0.切碎的棉花纤维;Gh_b.巯基乙酸和盐酸处理并清洗过的棉花纤维;Gh_b-0.用巯基乙酸和盐酸棉花纤维的吸收光谱减去切碎的棉花纤维的吸收光谱

Fig.2 FTIR spectrum of cotton fiber sample Note:Gh_0.The absorption spectrum of chopped cotton fiber; Gh_b.The absorption spectrum of cotton fiber treated with thioglycolic acid and HCl and washed by deionized water; Gh_b-0.The absorption spectrum of thioglycolic acid and HCl treated cotton fibers minus the absorption spectrum of chopped cotton fibers

图3 樟子松样品的FTIR光谱注:Pi_0.粉碎的樟子松粉末;Pi_b.巯基乙酸和盐酸处理并清洗过的樟子松粉末;Pi_b-0.用巯基乙酸和盐酸樟子松粉末的吸收光谱减去切碎的樟子松粉末的吸收光谱

Fig.3 FTIR spectrum of Pinus sylvestris L.var.mongolica Litv. Note:Pi_0.The absorption spectrum of powder of Pinus sylvestris L.var.mongolica Litv.; Pi_b.The absorption spectrum of powder of Pinus sylvestris L.var.mongolica Litv.treated with thioglycolic acid and HCl and washed by deionized water; Pi_b-0.The absorption spectrum of thioglycolic acid and HCl treated powder of Pinus sylvestris L.var.mongolica Litv.minus the absorption spectrum of untreated powder of Pinus sylvestris L.var.mongolica Litv

图4 切碎、巯基乙酸和盐酸处理棉花纤维细胞壁1 800~1 550 cm-1的FTIR光谱、光谱值小提琴图注:A.切碎棉花纤维(Gh_0)和巯基乙酸和盐酸处理的棉花纤维(Gh_b) 1 800~1 550 cm-1的光谱(n=30);B.光谱值小提琴图: 显示数据分布,****表示极显著差异

Fig.4 FTIR spectrum, violin plot of spectral values at 1,800-1,550 cm-1 between chopped and thioglycolic acid and HCl treated cotton fiber cell wall Note:A.Spectral (n=30) of chopped cotton fiber (Gh_0) and thioglycolic acid and HCl reated cotton fiber (Gh_b) at 1,800-1,550 cm-1; B.Violin plot of spectral values: showing data distribution, **** indicates extremely significant difference

图5 切碎、巯基乙酸和盐酸处理棉花纤维细胞壁1 320~1 200 cm-1的FTIR光谱、光谱值小提琴图 A.切碎棉花纤维(Gh_0)和巯基乙酸和盐酸处理的棉花纤维(Gh_b) 1 320~1 200 cm-1的光谱(n=30);B.光谱值小提琴图: 显示数据分布,****表示极显著差异

Fig.5 FTIR spectrum, violin plot of spectral values at 1,320-1,200 cm-1 between chopped and thioglycolic acid and HCl treated cotton fiber cell wall A.Spectral (n=30) of chopped (Gh_0) and thioglycolic acid and HCl reated cotton fiber (Gh_b) at 1,320-1,200 cm-1; B.Violin plot of spectral values: showing data distribution, **** indicates extremely significant difference

图6 粉碎、巯基乙酸和盐酸处理樟子松1 540~1 100 cm-1的FTIR光谱、光谱值小提琴图 A.粉碎樟子松(Pi_0)(n=12)和巯基乙酸和盐酸处理的樟子松(Pi_b) 1 540~1 100 cm-1的光谱(n=9);B.光谱值小提琴图: 显示数据分布,**表示显著差异

Fig.6 FTIR spectrum, violin plot of spectral values at 1,540-1,100 cm-1 between pulverized and thioglycolic acid and HCl treated Pinus sylvestris L.var.mongolica Litv A.Spectral of pulverized (Pi_0) (n= 12) and thioglycolic acid and HCl reated Pinus sylvestris L.var.mongolica Litv. (Pi_b) (n=9) at 1,540~1,100 cm-1; B.Violin plot of spectral values: showing data distribution, ** indicates significant difference

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