Determination of nutrient components and structural analysis of polysaccharides from wild Boletus

doi:10.6048/j.issn.1001-4330.2024.10.023

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (10): 2557-2565.DOI: 10.6048/j.issn.1001-4330.2024.10.023

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Determination of nutrient components and structural analysis of polysaccharides from wild Boletus

ZHANG Xinyu¹^,²(), Munire Mutalifu¹^,², YE Yijie¹^,², FENG Qian¹^,²^,³, ZHANG Zhidong²()

1. College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Special Environmental Microbiology, Urumqi 830091, China
3. Food Safety Testing Center of Aksu Region, Aksu Xinjiang 843000, China

Received:2024-03-11 Online:2024-10-20 Published:2024-11-07
Correspondence author: ZHANG Zhidong
Supported by:
Sub-project of the third comprehensive scientific investigation in Xinjiang(2022xjkk1200);The Project of Fund for Stable Support to Agricultural Sci-Tech Renovation(xjnkywdzc-2022005-6);Agricultural Science and Technology Innovation Platform Capacity Improvement Project of Xinjiang Academy of Agricultural Sciences(nkypt005);Agricultural Sciences Independent Cultivation Project Team Construction Project of Xinjiang Academy of Agricultural Sciences(nkyzztd-001)

野生牛肝菌营养成分测定及其多糖的结构分析

张芯语¹^,²(), 木尼热·木塔力甫¹^,², 叶艺洁¹^,², 冯倩¹^,²^,³, 张志东²()

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052
2.新疆农业科学院微生物应用研究所/新疆特殊环境微生物重点实验室,乌鲁木齐 830091
3.阿克苏地区食品安全检测中心,新疆阿克苏 843000

通讯作者: 张志东
作者简介:张芯语(1999-),女,河南人,硕士研究生,研究方向食品加工与安全,(E-mail)1807835109@qq.com
基金资助:
第三次新疆综合科学考察子课题(2022xjkk1200);新疆农业科学院农业科技创新稳定支持专项(xjnkywdzc-2022005-6);新疆农业科学院农业科技创新平台能力提升建设专项(nkypt005);新疆农业科学院自主培育项目团队建设专项(nkyzztd-001)

Abstract

Abstract:

【Objective】 In order to grasp the taxonomic status of Aksu wild Boletus, clarify its nutritional composition and analyze the main polysaccharide functional structure. 【Methods】 Using morphological and molecular biology methods to identify wild Boletus, analyze the nutritional components and minerals in Boletus for detection. The crude polysaccharide of Boletus pumila was extracted using water extraction and alcohol precipitation method, and purified using DEAE-52 cellulose column chromatography. Then, its monosaccharide composition was analyzed using high-pressure hydraulic chromatography. 【Results】 The wild Boletus from Aksu belongs to the order Agaricularia, family Boletus, and genus Boletus, and is identified by molecular identification as Leccinum duriusculum. Its main nutrients are sugars and proteins, accounting for 57.57% and 27.32% respectively. Three polysaccharide components were obtained through column chromatography combined with medium pressure chromatography system separation: LD-1, LD-2, and LD-3. Among them, LD-1 had the highest extraction rate of 78.8% and had strong water solubility. Infrared spectroscopy showed that it contained carbohydrate compounds with the highest proportion of glucose. 【Conclusion】 Wild Boletus in Aksu region is rich in polysaccharides and has strong water holding capacity. This result can provide scientific reference for the in-depth development and utilization of wild Boletus polysaccharides.

Key words: Boletus; nutrient composition; mineral elements; polysaccharide; isolation and purification

摘要：

【目的】 研究新疆阿克苏地区野生牛肝菌分类地位,分析其营养成分组成和解析主要多糖功能结构,为野生牛肝菌多糖的深入开发及利用提供科学参考。【方法】 利用形态学和分子生物学手段对野生牛肝菌进行鉴定,分析牛肝菌中的营养成分和矿物质进行检测。采用水提醇沉法提取皱皮疣柄牛肝菌粗多糖,并利用DEAE-52纤维素柱层析进行纯化,利用高压液压色谱分析其单糖组成。【结果】 阿克苏地区野生牛肝菌归属为伞菌目,牛肝菌科,疣柄牛肝菌属,分子鉴定为皱皮疣柄牛肝菌(Leccinum duriusculum)。其主要的营养物质为糖类和蛋白质,分别占57.57%和27.32%。通过柱层析联合中压色谱系统分离的手段获得了三种多糖组分为LD-1、LD-2和LD-3,其中LD-1的提取率最高,为78.8%,有较强的水溶性,红外光谱表明其含有糖类化合物,葡萄糖占比最高。【结论】 阿克苏地区野生牛肝菌富含多糖,持水力强。

关键词: 牛肝菌, 营养成分, 矿物质, 多糖, 分离纯化

CLC Number:

S646

ZHANG Xinyu, Munire Mutalifu, YE Yijie, FENG Qian, ZHANG Zhidong. Determination of nutrient components and structural analysis of polysaccharides from wild Boletus[J]. Xinjiang Agricultural Sciences, 2024, 61(10): 2557-2565.

张芯语, 木尼热·木塔力甫, 叶艺洁, 冯倩, 张志东. 野生牛肝菌营养成分测定及其多糖的结构分析[J]. 新疆农业科学, 2024, 61(10): 2557-2565.

Figures/Tables 9

Fig.1 Wild boletus sample morphology

Fig.2 Neighbor-Joining phylogenetic tree of ITS sequence of Leccinum duriusculum fruiting bodies

Fig.3 The standard curve of protein

Fig.4 The eluted curve with 0~0.2moL/L NaCl of LEP on DEAE-52 cellulose

Fig.5 Picture of LD(A)、LD-1(B)、LD-2(C)、LD-3(D)

Tab.1 Hydration properties of different polysaccharide components

特性 Character	多糖组分 Polysaccharide components
特性 Character	LD-1	LD-2	LD-3
水溶性 Water solubility (%)	61.00±2.5^a	16.33±0.72^c	35.66±1.69^b
持水力 Water holding capacity(g/g)	5.30±0.15^c	24.06±1.53^a	10.50±0.57^b

Fig.6 UV spectra of polysaccharide from LD-1、LD-2、LD-3

Fig.7 FTIR spectra of LD-1(A)、LD-2(B)、LD-3(C)

Tab.2 LD-1 of Monosaccharide Component

LD-1	葡萄糖 Glucose	鼠李糖 L-rham nose monohy drate	果糖 Fructose	阿拉伯糖 arabi nose	其他单糖 Other simple sugars
质量浓度 Mass concentration (mg/mL)	4.25	0.25	0.16	0.06	0.28
占比 Proportion (%)	85	5	3.2	1.2	5.6

References 27

[1]	ZHANG L, HU Y, DUAN X Y, et al. Characterization and antioxidant activities of polysaccharides from thirteen boletus mushrooms[J]. International Journal of Biological Macromolecules, 2018, 113: 0141-8130.
[2]	李泰辉, 宋斌. 中国食用牛肝菌的种类及其分布[J]. 食用菌学报, 2002, (2): 22-30.
	LI Taihui, SONG Bin. The Species and Distribution of Edible Boletus in China[J]. Acta Edulis Fungi, 2002, (2): 22-30.
[3]	彭秋菊, 严江, 陈国平, 等. 牛肝菌挥发性风味物质的研究进展[J]. 食品科技, 2022, 47(6): 127-132,139.
	PENG Qiujiu, YAN Jiang, CHEN Guoping, et al. Research on Volatile Flavor Compounds of Boletus[J]. Food Technology, 2022, 47(6): 127-132,139.
[4]	贾莉, 刘江华. 阿克苏地区林下经济发展现状与前景—以皱盖疣柄牛肝菌为例[J]. 林业科技通讯, 2023, (3): 1-5.
	JIA Li, LIU Jianghua. Current situation and prospect of economic development in the understory of Aksu area: A case study of Leccinum rugosiceps[J]. Forest Science and Technology, 2023, (3): 1-5.
[5]	樊莹润, 郑婷婷, 李泽林, 等. 黄皮疣柄牛肝菌多糖结构鉴定及对小鼠盲肠与粪便中短链脂肪酸的影响[J]. 食品科学, 2022, 43(11): 130-136.
	FAN Yingrun, ZHENG Tingting, LI Zelin, et al. Structural Identiﬁcation of Leccinum crocipodium (Letellier.) Watliag Polysaccharide and Its Effect on Short Chain Fatty Acid Contents in Cecum Contents and Feces of Mice[J]. Food Science, 2022, 43(11): 130-136.
[6]	郑金玲. 铜色牛肝菌多糖的制备及其小鼠体内抗肿瘤活性的研究[D]. 昆明: 昆明理工大学, 2022.
	Jinling Zheng. Preparation of Boletus aereus polysaccharides and their anti-tumor activity in mice[D]. Kunming: Kunming University of Science and Technology, 2022.
[7]	郑俏然, 张恒, 李文峰, 等. 美味牛肝菌多糖对急性肝损伤小鼠的保肝作用[J]. 食品与机械, 2019, 35(12): 141-145.
	ZHENG Qiaoran, ZHANG Heng, LI Wenfeng, et al. Protective effect of polysaccharides from delicious Boletus edulis on acute liver injury in mice[J]. Food and Machinery, 2019, 35(12): 141-145.
[8]	ZHENG J L, ZHANG T T, FAN J, et al. Protective effects of a polysaccharide from Boletus aereus on S180 tumor-bearing mice and its structural characteristics[J]. International journal of biological macromolecules, 2021, 188: 0141-8130.
[9]	Yu S S, Ma R J, Dong X D, et al. A novel polysaccharide from Boletus edulis: Extraction, purification, characterization and immunologic activity[J]. Industrial Crops and Products, 2022, 186: 0926-6690.
[10]	郑婷婷, 严亮, 张文杰, 等. 水碱连续提取黄皮疣柄牛肝菌粗多糖的理化性质及抗氧化活性研究[J]. 食品工业科技, 2020, 41(15): 84-89.
	ZHENG Tingting, YAN Liang, ZHANG Wenjie, et al. Physicochemical Properties and Antioxidant Activity of Water-Alkali Continuous Extraction of Crude Polysaccharides from Leccinellum crocipodium(Letellier.) Watliag[J]. Science and Technology of Food Industry, 2020, 41(15): 84-89.
[11]	DU R P, XING H W, YANG Y P, et al. Optimization, purification and structural characterization of a dextran produced by L. mesenteroides isolated from Chinese sauerkraut[J]. Carbohydrate polymers, 2017, 174: 409-416.
[12]	粟思源. 美网柄牛肝菌的鉴定及其多糖(BRS-X)的结构鉴定、免疫活性及抗肿瘤活性机制的研究[D]. 南充: 西华师范大学, 2019.
	Siyuan SU. Identification of Boletus edulis and its polysaccharide (BRS-X) structure, immune activity, and anti-tumor activity mechanism[D]. Nanchong: China West Normal University, 2019.
[13]	曾丽萍, 王心诗, 吴素蕊, 等. 云南铜色牛肝菌多糖分离纯化及抗氧化活性研究[J]. 食品工业科技, 2016, 37(4): 151-154.
	ZENG Liping, WANG Xinshi, WU Surui, et al. Purification and antioxidant activity of polysaccharide from Boletus aereus in Yunnan[J]. Science and Technology of Food Industry, 2016, 37(4): 151-154.
[14]	赵圆圆. 杏鲍菇子实体多糖的提取纯化、结构表征及降脂活性研究[D]. 西安: 陕西科技大学, 2020.
	Yuanyuan ZHAO. Study on Extraction, Purification, Structure Characterization and Lipid-lowering Activity of Polysaccharide from Pleurotus eryngi Fruit Body[D]. Shaanxi: Shaanxi University of Science Technology, 2020.
[15]	程毛, 邢莉丽, 江吉德. 不同干燥方式对柚子皮多糖理化特性及功能活性的影响[J]. 食品科技, 2021, 46(4): 162-167.
	CHENG Mao, XING Lili, JIANG Jide. Effects of Different Drying Methods on the Physicochemical Properties and Functional Activities of Polysaccharides from Grapefruit Peel[J]. Food Science and Technology, 2021, 46(4): 162-167.
[16]	陈超. 榛蘑多糖的提取、纯化及体外降血脂研究[D]. 哈尔滨市: 东北农业大学, 2017.
	Chao CHEN. Extraction Purification and Lowing Pllasma Cholesterol in Vitro of Polysaccharide from Armillaria Mellea[D]. Harbin: Northeast Agricultural University, 2017.
[17]	张俊霞. 连钱草多糖分离纯化、结构分析及生物活性初探[D]. 恩施: 湖北民族学院, 2017.
	Junxia ZHANG. Isolation,Purification,Structural Analysis and Bioactivities of Polysaccharides from Glechoma longituba(Nakai)Kupr.[D]. Enshi: Hubei Institute For Nationalities, 2017.
[18]	刘雅欣, 贾鹏, 王虹, 等. 乙醇分级沉淀黄芪多糖免疫活性的评价[J]. 中成药, 2020, 42(10): 2729-2732.
	LIU Yaxin, JIA Peng, WANG Hong, et al. Evaluation of immune activity of Astragalus polysaccharide by ethanol fractional precipitation[J]. Chinese Patent Medicine, 2020, 42(10): 2729-2732.
[19]	文春玉, 徐明, 杨云礼, 等. 我国野生食用菌营养成分与价值评价研究进展[J]. 中国食用菌, 2021, 40(11): 1-10,20.
	WEN Chunyu, XU Ming, YANG Yunli, et al. Research Progress on Nutritional Components and Value Evaluation of Wild Edible Fungi in China[J]. EDIBLE FUNGI OF CHINA, 2021, 40(11): 1-10,20.
[20]	普布多吉, 王科, 马超, 等. 西藏牛肝菌物种资源概述[J]. 食用菌学报, 2018, 25(2): 137-165.
	Pubuduoji, WANG Ke, MA Chao, et al. Overview of bolus fungus species resources in Tibet[J]. Journal of Edible Fungi, 2018, 25(2): 137-165.
[21]	周强, 马德英, 林辰壹, 等. 新疆额尔齐斯河沿岸野生牛肝菌菌种的分离与鉴定[J]. 新疆农业科学, 2016, 53(1): 176-184.
	ZHOU Qiang, MA Deying, LIN Chenyi, et al. Isolation and identification of wild boletus strains along the Irtysh River in Xinjiang[J]. Xinjiang Agricultural Sciences, 2016, 53(1): 176-184.
[22]	马长中, 徐锦华, 张景荣, 等. 林芝橙黄疣柄牛肝菌营养成分分析与评价[J]. 食品与发酵工业, 2018, 44(5): 259-264.
	MA Changzhong, XU Jinhua, ZHANG Jingrong, et al. Analysis and evaluation of nutritional components of wild Leccinum aurantiacum(Bull.et Pers.)Gray from Linzhi[J]. Food and Fermentation Industries, 2018, 44(5): 259-264.
[23]	刘秋鸣, 李笑, 肖俊江, 等. 云南产黄皮疣柄牛肝菌基本营养成分和抗氧化活性[J]. 食品工业科技, 2018, 39(16): 275-280.
	LIU Qiuming, LI Xiao, XIAO Junjiang, et al. Nutritional Components and Antioxidant Activities of Leccinum crocipodium in Yunnan[J]. Food Industry Science and Technology, 2018, 39(16): 275-280.
[24]	张春霞, 纪开萍, 何明霞, 等. 暗褐网柄牛肝菌子实体营养成分分析[J]. 云南大学学报(自然科学版), 2010, 32(6): 702-704.
	ZHANG Chunxia, JI Kaiping, HE Mingxia, et al. Analysis of nutritional constituents in the fruiting bodies of Phlebopus portentosus[J]. Journal of Yunnan University, 2010, 32(6): 702-704.
[25]	邓雅元, 游金坤, 华蓉, 等. 3种常见野生牛肝菌和3种大宗人工食用菌营养成分分析[J]. 中国食用菌, 2022, 41(3): 45-48.
	DENG Yayuan, YOU Jinkun, HUA Rong, et al. Nutritional Analysis of Three Common Wild Boltus spp. and Three Staple Artificial Edible Fungi[J]. Edlble Fungi Of China, 2022, 41(3): 45-48.
[26]	董芳. 皱盖疣柄牛肝菌多糖结构分析及生物活性研究[D]. 广州: 华南理工大学, 2018.
	Fang DONG. Study on Structure and Bioactivities of Polysaccarides from Leccinum rugosiceps[D]. Guangzhou: South China University of Technology, 2018.
[27]	伍燕, 徐峰, 李小双, 等. 皱盖疣柄牛肝菌多糖LRP-I和LRP-II结构表征及抗氧化活性分析[J]. 食品工业科技, 2020, 41(21): 8-14.
	WU Yan, XU Feng, LI Xiaoshuang, et al. Structural Elucidation and Antioxidant Activity of LRP-I and LRP-II Polysaccharide from Leccinum rugosiceps[J]. Food Industry Science and Technology, 2020, 41(21): 8-14.

Determination of nutrient components and structural analysis of polysaccharides from wild Boletus

野生牛肝菌营养成分测定及其多糖的结构分析

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