Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (2): 469-478.DOI: 10.6048/j.issn.1001-4330.2024.02.024
• Microbes·Physiology and Biochemistry·Animal Husbandry Veterinarian • Previous Articles Next Articles
ZHANG Mengyuan1(), ZHU Xiaoqing1(
), GU Xinli1(
), WANG Yan1, YANG Huiying1, LIU Yuran1, WANG Mengmeng1, SUN Guojie1, LIAN Kexun1, LI Xiaozhen2
Received:
2023-06-18
Online:
2024-02-20
Published:
2024-03-19
Correspondence author:
ZHU Xiaoqing(1981-), female, from Jinta, Gansu, Ph.D., Lecturer, research interests in Chinese medicine immunopharmacology,(E-mail)Supported by:
张梦圆1(), 朱晓庆1(
), 谷新利1(
), 王燕1, 杨慧莹1, 刘雨然1, 王蒙蒙1, 孙国结1, 连科迅1, 李效振2
通讯作者:
朱晓庆(1981-),女,甘肃金塔人,讲师,博士,研究方向为中药免疫药理学,(E-mail)作者简介:
张梦圆(1996-),女,安徽阜阳人,硕士研究生,研究方向为中药免疫药理学, (E-mail)330941027@qq.com
基金资助:
CLC Number:
ZHANG Mengyuan, ZHU Xiaoqing, GU Xinli, WANG Yan, YANG Huiying, LIU Yuran, WANG Mengmeng, SUN Guojie, LIAN Kexun, LI Xiaozhen. Study on antibacterial activity and mechanism of selenium glycyrrhiza polysaccharide, glycyrrhiza polysaccharide and their combined antibiotics against Streptococcus agalactiae in vitro[J]. Xinjiang Agricultural Sciences, 2024, 61(2): 469-478.
张梦圆, 朱晓庆, 谷新利, 王燕, 杨慧莹, 刘雨然, 王蒙蒙, 孙国结, 连科迅, 李效振. 硒化甘草多糖、甘草多糖及其联合抗生素对无乳链球菌体外抗菌活性及机制分析[J]. 新疆农业科学, 2024, 61(2): 469-478.
药物名称 Drug name | 抑菌圈直径 Diameter of bacteriostatic ring(mm) |
---|---|
阿莫西林Amoxicillin | 17.0 |
头孢曲松钠Ceftriaxone sodium | 27.0 |
盐酸卡那霉素Kanamycin hydrochloride | 22.0 |
盐酸多西环素Doxycycline hydrochloride | 26.0 |
链霉素streptomycin | 1.5 |
氟苯尼考Florfenicol | 28.0 |
SeGUP 120 mg/mL | 9.0 |
SeGUP 240 mg/mL | 12.0 |
SeGUP 480 mg/mL | 14.0 |
GUP 120 mg/mL | 6.0 |
GUP 240 mg/mL | 9.0 |
GUP 480 mg/mL | 11.0 |
Tab.1 Determination of SeGUP and GUP on the diameter of inhibition zone of Streptococcus agalactis
药物名称 Drug name | 抑菌圈直径 Diameter of bacteriostatic ring(mm) |
---|---|
阿莫西林Amoxicillin | 17.0 |
头孢曲松钠Ceftriaxone sodium | 27.0 |
盐酸卡那霉素Kanamycin hydrochloride | 22.0 |
盐酸多西环素Doxycycline hydrochloride | 26.0 |
链霉素streptomycin | 1.5 |
氟苯尼考Florfenicol | 28.0 |
SeGUP 120 mg/mL | 9.0 |
SeGUP 240 mg/mL | 12.0 |
SeGUP 480 mg/mL | 14.0 |
GUP 120 mg/mL | 6.0 |
GUP 240 mg/mL | 9.0 |
GUP 480 mg/mL | 11.0 |
药物名称 Drug name | 单用MIC Signle use MIC | MBC | 与SeGUP 联用后抗 生素浓度 Antibiotic concentration afer combination with SeGUP | 联用后 SeGUP 浓度 Concentration of SeGUP after combinet use | FICI | 关系 Relation- ship | 与GUP联用 后抗生素 浓度 Antibiotic concentration afer combination with GUP | 联用后 GUP浓度 Concentration of GUP after combinet use | FICI | 关系 Relation- ship |
---|---|---|---|---|---|---|---|---|---|---|
盐酸卡那霉素 Kanamycin hydrochloride (μg/mL) | 31.25 | 62.5 | 15.63 | 120 | 1.5 | 无关 | 15.63 | 240 | 1.5 | 无关 |
盐酸多西环素 Doxycycline hydrochloride (μg/mL) | 15.63 | 31.25 | 3.91 | 60 | 0.75 | 相加 | 7.81 | 120 | 1 | 相加 |
头孢曲松钠 Ceftriaxone sodium (μg/mL) | 62.5 | 125 | 31.25 | 60 | 1 | 相加 | 31.25 | 240 | 1.5 | 无关 |
氟苯尼考 Florfenicol μg/mL | 31.25 | 62.5 | 7.81 | 30 | 0.5 | 协同 | 7.81 | 120 | 0.75 | 相加 |
SeGUP (mg/mL) | 120 | 120 | ||||||||
GUP (mg/mL) | 240 | 480 |
Tab.2 Determination results of MIC, MBC and FICI of single drug against Streptococcus agalactis
药物名称 Drug name | 单用MIC Signle use MIC | MBC | 与SeGUP 联用后抗 生素浓度 Antibiotic concentration afer combination with SeGUP | 联用后 SeGUP 浓度 Concentration of SeGUP after combinet use | FICI | 关系 Relation- ship | 与GUP联用 后抗生素 浓度 Antibiotic concentration afer combination with GUP | 联用后 GUP浓度 Concentration of GUP after combinet use | FICI | 关系 Relation- ship |
---|---|---|---|---|---|---|---|---|---|---|
盐酸卡那霉素 Kanamycin hydrochloride (μg/mL) | 31.25 | 62.5 | 15.63 | 120 | 1.5 | 无关 | 15.63 | 240 | 1.5 | 无关 |
盐酸多西环素 Doxycycline hydrochloride (μg/mL) | 15.63 | 31.25 | 3.91 | 60 | 0.75 | 相加 | 7.81 | 120 | 1 | 相加 |
头孢曲松钠 Ceftriaxone sodium (μg/mL) | 62.5 | 125 | 31.25 | 60 | 1 | 相加 | 31.25 | 240 | 1.5 | 无关 |
氟苯尼考 Florfenicol μg/mL | 31.25 | 62.5 | 7.81 | 30 | 0.5 | 协同 | 7.81 | 120 | 0.75 | 相加 |
SeGUP (mg/mL) | 120 | 120 | ||||||||
GUP (mg/mL) | 240 | 480 |
药物Drug | AKP(ng/L) | β-gal(pg/mL) | 蛋白质 Protein(μg/mL) | DNA(ng/μL) |
---|---|---|---|---|
SeGUP 120 mg/mL | 202.97±4.32b | 105.43±2.51e | 640.40±4.62e | 58.63±2.06j |
SeGUP 60 mg/mL | 173.07±3.67e | 94.70±2.34f | 532.70±7.41gh | 81.87±2.47h |
SeGUP 30 mg/mL | 143.37±2.14j | 75.37±2.05gh | 471.57±8.21j | 91.60±1.65g |
GUP 120 mg/mL | 182.43±2.56d | 92.80±1.87f | 542.47±2.77f | 82.37±2.04h |
GUP 60 mg/mL | 151.70±0.75i | 74.67±1.20ghi | 477.73±4.54j | 113.47±4.21de |
GUP 30 mg/mL | 104.70±2.80k | 53.67±2.91l | 384.43±6.96l | 153.37±3.95b |
1/2盐酸卡那霉素MIC Kanamycin hydrochloride | 158.40±4.20gh | 66.33±2.06k | 457.53±5.81k | 93.73±4.21g |
1/2盐酸多西环素MIC Doxycycline hydrochloride | 151.43±2.10i | 74.00±1.97hi | 471.67±7.75j | 108.67±2.78e |
1/2头孢曲松钠MIC Ceftriaxone sodium | 162.70±4.11g | 66.20±2.17k | 527.43±6.29h | 120.37±1.72c |
1/2氟苯尼考MIC Florfenicol | 153.63±3.65hi | 78.20±1.85i | 546.47±5.14f | 83.40±1.66h |
1/2(盐酸卡那霉素MIC+SeGUP MIC) | 163.23±5.58fg | 76.50±1.83gh | 504.00±4.97i | 100.27±6.12f |
1/2(盐酸多西环素MIC+SeGUP MIC) | 217.43±3.48a | 123.83±2.12bg | 748.70±4.28b | 47.30±1.87k |
1/2(头孢曲松钠MIC+SeGUP MIC) | 202.60±3.54b | 121.00±2.35bc | 705.57±4.15c | 51.70±2.15k |
1/2(氟苯尼考MIC+SeGUP MIC) | 219.43±2.61a | 129.57±2.10a | 789.37±1.46a | 57.40±2.40j |
1/2(盐酸卡那霉素MIC+GUP MIC) | 168.93±1.65ef | 68.30±1.90jk | 462.60±5.20k | 117.70±2.91cd |
1/2(盐酸多西环素MIC+GUP MIC) | 197.90±5.45bc | 119.57±2.06c | 679.93±1.38d | 61.37±2.23ij |
1/2(头孢曲松钠MIC+GUP MIC) | 155.27±1.88hi | 71.07±2.70ij | 539.03±1.76fg | 118.87±2.90cd |
1/2(氟苯尼考MIC+GUP MIC) | 193.13±5.05c | 115.50±3.10d | 713.23±3.35c | 66.30±2.95i |
空白对照组 | 29.00±1.15l | 31.67±1.96m | 102.00±3.25m | 215.00±4.56a |
Tab.3 Effect of SeGUP, GUP and combined antibiotics on bacterial effects of AKP β-gal, protein and intracellular DNA
药物Drug | AKP(ng/L) | β-gal(pg/mL) | 蛋白质 Protein(μg/mL) | DNA(ng/μL) |
---|---|---|---|---|
SeGUP 120 mg/mL | 202.97±4.32b | 105.43±2.51e | 640.40±4.62e | 58.63±2.06j |
SeGUP 60 mg/mL | 173.07±3.67e | 94.70±2.34f | 532.70±7.41gh | 81.87±2.47h |
SeGUP 30 mg/mL | 143.37±2.14j | 75.37±2.05gh | 471.57±8.21j | 91.60±1.65g |
GUP 120 mg/mL | 182.43±2.56d | 92.80±1.87f | 542.47±2.77f | 82.37±2.04h |
GUP 60 mg/mL | 151.70±0.75i | 74.67±1.20ghi | 477.73±4.54j | 113.47±4.21de |
GUP 30 mg/mL | 104.70±2.80k | 53.67±2.91l | 384.43±6.96l | 153.37±3.95b |
1/2盐酸卡那霉素MIC Kanamycin hydrochloride | 158.40±4.20gh | 66.33±2.06k | 457.53±5.81k | 93.73±4.21g |
1/2盐酸多西环素MIC Doxycycline hydrochloride | 151.43±2.10i | 74.00±1.97hi | 471.67±7.75j | 108.67±2.78e |
1/2头孢曲松钠MIC Ceftriaxone sodium | 162.70±4.11g | 66.20±2.17k | 527.43±6.29h | 120.37±1.72c |
1/2氟苯尼考MIC Florfenicol | 153.63±3.65hi | 78.20±1.85i | 546.47±5.14f | 83.40±1.66h |
1/2(盐酸卡那霉素MIC+SeGUP MIC) | 163.23±5.58fg | 76.50±1.83gh | 504.00±4.97i | 100.27±6.12f |
1/2(盐酸多西环素MIC+SeGUP MIC) | 217.43±3.48a | 123.83±2.12bg | 748.70±4.28b | 47.30±1.87k |
1/2(头孢曲松钠MIC+SeGUP MIC) | 202.60±3.54b | 121.00±2.35bc | 705.57±4.15c | 51.70±2.15k |
1/2(氟苯尼考MIC+SeGUP MIC) | 219.43±2.61a | 129.57±2.10a | 789.37±1.46a | 57.40±2.40j |
1/2(盐酸卡那霉素MIC+GUP MIC) | 168.93±1.65ef | 68.30±1.90jk | 462.60±5.20k | 117.70±2.91cd |
1/2(盐酸多西环素MIC+GUP MIC) | 197.90±5.45bc | 119.57±2.06c | 679.93±1.38d | 61.37±2.23ij |
1/2(头孢曲松钠MIC+GUP MIC) | 155.27±1.88hi | 71.07±2.70ij | 539.03±1.76fg | 118.87±2.90cd |
1/2(氟苯尼考MIC+GUP MIC) | 193.13±5.05c | 115.50±3.10d | 713.23±3.35c | 66.30±2.95i |
空白对照组 | 29.00±1.15l | 31.67±1.96m | 102.00±3.25m | 215.00±4.56a |
Fig.3 Effect of SeGUP and GUP on biofilm formation of Streptococcus pneumonia Note:Numbers with the same shoulder markers or no letters indicate that the differences are not significant(P > 0.05),Different letters represent significant differences(P < 0.05), the same as below
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