Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region

doi:10.6048/j.issn.1001-4330.2023.07.030

Abstract

Abstract:

【Objective】 To optimize the extraction conditions of polysaccharides from the wild boletus in Aksu region and determine the physical properties and antioxidant capacity of different polysaccharide components. 【Methods】 The wild boletus was sampled from Aksu region,and water extraction conditions of polysaccharide were optimized. Two polysaccharide components,LD-40 and LD-80,were obtain by fractional precipitation of ethanol with volume fraction of 40% and 80%. The polysaccharide properties of water solubility,water retention and viscosity were tested. The antioxidant capacity was evaluated by free radical scavenging of DPPH and hydroxyl and reducing power experiments. 【Results】 The optimal extraction conditions were solid-liquid ratio 1∶30,temperature at 80℃,time for 3 h,then the yield of polysaccharide was 13.36%. Polysaccharide components,LD-40 and LD-80,obtained from ethanol fractional precipitation accounted for 92.37% and 7.63% of the total polysaccharide,respectively. The water solubility of LD-40 and LD-80 was 66.9% and 74.9%,respectively,and the water holding capacities were 7.71 and 6.16 g/g,respectively. At the same concentration,the viscosity of LD-40 was stronger than that of LD-80,but LD-80 had stronger antioxidant capacity than LD-40.【Conclusion】 The wild boletus in Aksu region is rich in polysaccharides which has good water holding capacity,but the antioxidant activity is weak. The results can provide scientific reference for the further development and utilization of wild boletus polysaccharides.

Key words: boletus; polysaccharide; fractional precipitation

摘要：

【目的】研究阿克苏野生牛肝菌多糖的最佳提取工艺,分析不同多糖组分的理化性质和抗氧化能力,为野生牛肝菌多糖的深入开发及利用提供科学参考。【方法】以阿克苏地区野生牛肝菌为材料,优化多糖水提条件,采用体积分数为40% 和 80% 的乙醇进行分级沉淀,得到2种粗多糖组分 LD-40 和 LD-80;测定多糖的水溶性、持水力和粘度,清除DPPH自由基、羟基自由基进行还原力试验,评价2种多糖组分的特性。【结果】牛肝菌多糖的最佳提取工艺条件为料液比1:30,温度80℃,时间3 h,多糖最高得率为13.36%。乙醇分级沉淀获得LD-40和 LD-80分别占总多糖的92.37%和7.63%,各自水溶性分别为66.9%和74.9%,持水力为7.71和6.16 g/g。相同浓度下,LD-40粘度较LD-80更强,但LD-80抗氧化能力比LD-40强。【结论】阿克苏地区野生牛肝菌富含多糖,具有较好的持水力,但抗氧化活性较弱。

关键词: 牛肝菌, 多糖, 分级醇沉

CLC Number:

S567.3

FENG Qian, HE Qi, LI Xue, YI Yuanyang, SUN Jian, ZHU Jing, GU Meiying, TAN Huilin, ZHANG Zhidong. Extraction and antioxidant activity of different polysaccharide components from the wild boletus in Aksu region[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1813-1820.

冯倩, 何齐, 李雪, 易鸳鸯, 孙建, 朱静, 顾美英, 谭慧林, 张志东. 阿克苏野生牛肝菌多糖提取优化及不同组分抗氧化活性分析[J]. 新疆农业科学, 2023, 60(7): 1813-1820.

Figures/Tables 12

Fig.1 Wild boletus under the poplar forest in Aksu Region

Tab.1 Factors and levels of orthogonal tests for extraction optimization of polysaccharide from the wild Boletus

水平 Level	因素Factor
水平 Level	A料液比 (g:mL)	B提取温度 (℃)	C提取时间 (h)
1	1∶20	75	2
2	1∶25	80	3
3	1∶30	85	4

Fig.2 Standard curve for polysaccharide determination

Fig.3 Effect of extraction temperature on the yield of polysaccharides

Fig.4 Effect of solid-liquid ratio on the yield of polysaccharides

Fig.5 Effect of extraction time on the yield of polysaccharides

Tab.2 Results and analysis of orthogonal tests for extraction conditions optimization of polysaccharides from Boletus

试验 Test	A	B	C	多糖得率 Polysaccharide yield(%)
1	1	1	1	8.58
2	1	2	2	12.77
3	1	3	3	11.25
4	2	1	2	12.92
5	2	2	3	11.16
6	2	3	1	10.70
7	3	1	3	11.33
8	3	2	1	11.75
9	3	3	2	11.86
k₁	10.867	10.943	10.343
k₂	11.593	11.893	12.517
k₃	11.647	11.27	11.247
极差R Range	0.78	0.95	2.174

Tab.3 Hydration properties of different polysaccharide components

特性 Character	多糖组分 Polysaccharide components
特性 Character	LD-40	LD-80
水溶性 Water solubility(%)	66.6±3.45	74.9±2.35
持水力 Water holding capacity(g/g)	7.71±0.37	6.16±0.43

Fig.6 Viscosity of each polysaccharide component at different concentrations

Fig.7 Effects of polysaccharide components at different concentrations on DPPH radicals scavenging activity

Fig.8 Effects of polysaccharide components at different concentrations on OH radicals scavenging activity

Fig.9 Reducing power of the polysaccharide components at different concentrations

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