Effects of Chinese Herbal Compound Polysaccharides on Antibody Potency of Avian Influenza in Different MHC B-LβII Genotypes of Laying Hens

doi:10.6048/j.issn.1001-4330.2017.12.021

Xinjiang Agricultural Sciences ›› 2017, Vol. 54 ›› Issue (12): 2329-2336.DOI: 10.6048/j.issn.1001-4330.2017.12.021

Effects of Chinese Herbal Compound Polysaccharides on Antibody Potency of Avian Influenza in Different MHC B-LβII Genotypes of Laying Hens

LIU Xiao-ting, ZHU Xiao-qing, MA Zhao, QIAO Yan-jie, ZHANG Bao-jun, GU Xin-li, SHANG Yun-xia

College of Animal Science and Technology, Shihezi University, Shihezi Xinjiang 832003, China

Received:2017-10-11 Online:2017-12-25 Published:2018-01-29
Correspondence author: GU Xin-li(1963-),male, native place: Xuchang Henan, doctoral tutor, doctor, research field: development and application of traditional Chinese medicine preparation, (E-mail)xlgu@shzu.edu.cn;SHANG Yun-xia(1965-),femal, native place: Ruyang Henan, senior experimentalist, bachelor, research field: prevention and treatment of animal diseases, (E-mail)shangyunxia@ shzu.edu.cn
Supported by:
he National Natural Science Foundation of China (NSFC)"Effects and mechanism of certain purity compound Chinese herbal medicinal polysaccharide on immunomodulatory in different MHC B-LβII genotype chickens"(31460673)

中药复方多糖对不同MHC B-LβII基因型蛋鸡禽流感抗体效价的影响

刘晓婷, 朱晓庆, 马昭, 乔彦杰, 张保军, 谷新利, 商云霞

石河子大学动物科技学院,新疆石河子 832003

通讯作者: 谷新利(1963-),男,河南许昌人,博士生导师,博士,研究方向为中药制剂,(E-mail)xlgu@shzu.edu.cn;商云霞(1965-),女,河南汝阳人,高级实验师,研究方向为动物疫病防治,(E-mail)shangyunxia@shzu
作者简介:刘晓婷(1991-),女,河南濮阳人,硕士研究生,研究方向为中草药制剂,(E-mail)1341280217@qq.com
基金资助:
国家自然科学基金项目“一定纯度中药复方多糖对不同MHC B-LβII基因型鸡免疫调节作用及其作用机制研究”(31460673)

Abstract

Abstract: 【Objective】 This experiment was conducted to study the effects of intramuscular injection of compound polysaccharides on avian influenza antibody titers in serum of different MHC B-LβII genotype laying hens. 【Method】500 chickens were divided into three groups according to different genotypes by PCR-SSCP method. The genotypes were divided into high, medium and low dose Chinese herbal compound polysaccharide groups and blank control group. The test chickens of each group were intramuscularly injected with 50 mg / mL, 25 mg / mL, 12.5 mg / mL Chinese herbal compound polysaccharide and saline, 0.2 mL / day for 7 days. At the age of 27, 35, 42 and 49 days, 5 chickens were randomly selected from each group and vein blood was collected from the wings for separation of serum. The titer of H5 and H9 subtype avian influenza antibodies in serum was measured by microhialysis inhibition test (HI).【Result】The results showed that, before immunization, the average titer of H5N1 Re-6 and H9N2 maternal antibody was higher than that of the control group, and the titer of H5N1 maternal antibody in AA and BC genotype of high-dose Chinese herbal compound polysaccharide group was significantly higher than that of control group (P <0.05). On the 7 day after immunization, the titer of H5 subtype avian influenza antibody in AA genotype chickens in high, medium and low dose groups was significantly higher than that in control group (P <0.05), the titer of H5 subtype avian influenza antibody in BC genotype high dose group was significantly higher than that in control group (P <0.05) and the titer of H9 subtype avian influenza antibody in BC high, medium and low dose groups was significantly higher than that in control group (P <0.05); On the 14 days after immunization, the titer of H5 subtype avian influenza antibody of AA genotype high and medium dose group was significantly higher than that of control group (P <0.05), the BB genotype in the middle dose group was significantly higher than that in the control group (P <0.05) and the H9 genotype BC avian influenza in the middle dose group was significantly higher than that in the control group (P <0.05); On the 21 days after immunization, the genotype H5 avian influenza of BC genotype high and medium dose group was significantly higher than that of control group (P <0.05) and the avian influenza of BC genotype chicken high dose group was significantly higher than that of control group (P <0.05).【Conclusion】These results indicated that different doses of compound herbal Chinese medicine polysaccharide could improve the titer of H5 and H9 subtype avian influenza antibodies in serum of laying hens to certain degrees, and the medium dose and high dose are of good effect.

Key words: Chinese medicine compound polysaccharide; laying hens; MHC B-LβII gene; avian influenza antibody titer

摘要： 【目的】研究肌注中药复方多糖对不同MHC B-LβII基因型蛋鸡血清中禽流感抗体效价的影响。【方法】试验采用PCR-SSCP方法将500羽京红1号蛋鸡按不同基因型分组,再将各基因型组分成高、中、低剂量中药复方多糖组和空白对照组。各组试验鸡分别于8日龄肌注50、25和12.5 mg/mL中药复方多糖和生理盐水,0.2 mL/只,连续7 d,于27、35、42、49日龄从各组随机抽取5只试验鸡,翅下静脉采血并分离血清,用微量血凝抑制试验(HI)检测血清中H5与H9亚型禽流感抗体效价。【结果】免疫前,各剂量中药复方多糖组H5N1 Re-6和H9N2母源抗体平均效价高于对照组,且高剂量AA和BC基因型鸡血清中H5N1母源抗体效价显著高于对照组(P<0.05);免疫后7 d,AA基因型鸡高、中、低剂量组的H5亚型禽流感抗体效价均显著高于对照组(P<0.05),BC基因型鸡高剂量组H5亚型禽流感抗体效价显著高于对照组(P<0.05),BC基因型鸡高、中、低剂量组H9亚型禽流感抗体效价显著高于对照组(P<0.05);免疫后14 d,AA基因型鸡高、中剂量组H5亚型禽流感抗体效价显著高于对照组(P<0.05),BB基因型鸡中剂量组显著高于对照组(P<0.05),BC基因型鸡中剂量组H9亚型禽流感显著高于对照组(P<0.05);免疫后21 d,BC基因型鸡高、中剂量组H5亚型禽流感显著高于对照组(P<0.05),BC基因型鸡高剂量组显著高于对照组(P<0.05)。【结论】不同剂量中药复方多糖均能不同程度的提高各基因型蛋鸡血清中H5和H9亚型禽流感抗体效价,且以中剂量和高剂量效果好。

关键词: 中药复方多糖, 蛋鸡, MHC B-LβII基因, 禽流感抗体效价

CLC Number:

S852

LIU Xiao-ting, ZHU Xiao-qing, MA Zhao, QIAO Yan-jie, ZHANG Bao-jun, GU Xin-li, SHANG Yun-xia. Effects of Chinese Herbal Compound Polysaccharides on Antibody Potency of Avian Influenza in Different MHC B-LβII Genotypes of Laying Hens[J]. Xinjiang Agricultural Sciences, 2017, 54(12): 2329-2336.

刘晓婷, 朱晓庆, 马昭, 乔彦杰, 张保军, 谷新利, 商云霞. 中药复方多糖对不同MHC B-LβII基因型蛋鸡禽流感抗体效价的影响[J]. 新疆农业科学, 2017, 54(12): 2329-2336.

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Effects of Chinese Herbal Compound Polysaccharides on Antibody Potency of Avian Influenza in Different MHC B-LβII Genotypes of Laying Hens

中药复方多糖对不同MHC B-LβII基因型蛋鸡禽流感抗体效价的影响

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