Effects of Traditional Chinese Medicinal Polysaccharide Extraction on Chicken Lymphocyte of IL-2, IL-4 and IL-12 mRNA Expression in Different MHC B-LβII Genotype Chickens Lymphocyte

doi:10.6048/j.issn.1001-4330.2017.12.020

Xinjiang Agricultural Sciences ›› 2017, Vol. 54 ›› Issue (12): 2320-2328.DOI: 10.6048/j.issn.1001-4330.2017.12.020

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Effects of Traditional Chinese Medicinal Polysaccharide Extraction on Chicken Lymphocyte of IL-2, IL-4 and IL-12 mRNA Expression in Different MHC B-LβII Genotype Chickens Lymphocyte

MA Zhao, LIAN Ke-xun, LIU Gang, LIU Xiao-ting, QIAO Yan-jie, ZHU Xiao-qing, GU Xin-li

College of Animal Science and Technology, Shihezi University, Shihezi Xinjiang 832003, China

Received:2017-11-09 Online:2017-12-25 Published:2018-01-29
Correspondence author: ZHU Xiao-qin(1981-), femal, lecturer, Research area: Research and development of Chinese veterinary drug preparation, (E-mail)zxhqqmy@126.com;GU Xin-li(1963-), male, Doctoral Supervisor, Research area: Development and utilization of Chinese herbal medicine preparations, (E-mail)xlgu@shzu.edu.cn
Supported by:
Regional Science Fund Project "Effects and mechanism of certain purity compound Chinese herbal medicinal polysaccharide on immunomodulatory in different MHC B-LβII" (31460673)

中药复方多糖对不同MHC B-LβII基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响

马昭, 连科讯, 刘钢, 刘晓婷, 乔严杰, 朱晓庆, 谷新利

石河子大学动物科技学院,新疆石河子 832003

通讯作者: 朱晓庆(1981-),女,甘肃金塔人,博士,研究方向为中兽药制剂,(E-mail)zxhqqmy@126.com;谷新利(1963-),男,河南许昌人,教授,博士生导师,研究方向为中药制剂,(E-mail)xlgu@shzu.edu.cn
作者简介:马昭(1990-),男,甘肃会宁人,硕士研究生,研究方向为临床兽医学,(E-mail)1669443187@qq.com
基金资助:
地区科学基金项目“一定纯度中药复方多糖对不同MHC B-LβII基因型鸡免疫调节作用及其作用机制研究”(31460673)

Abstract

Abstract: 【Objective】 To explore the effect of polysaccharide extracted from traditional Chinese medicines on immunomodulatory in different MHC B Lβ II genotype chickens.【Method】PCR-SSCP technique was applied to group feather white feather chickens according to different MHC B-LβII genotype, the different MHC B-LβII genotypes were collected from white feather chickens and peripheral blood lymphocytes were isolated. cCHMPS of the final concentrations 100 μg/mL, 75 μg/mL, 50 μg/mL, 0 μg/mL was added and cultured together for 24 h, and then the expression of lymphocyte IL-2, IL-12, IL-4 mRNA was detected by using Real-time PCR method.【Result】(1) Traditional Chinese medicinal polysaccharide extraction of different doses could improve IL-2, IL-12, IL-4 mRNA expression levels of chicken lymphocyte compared with the control group in each MHC B Lβ II genotype chickens (P ＜0.05). (2) And when the traditional Chinese medicines concentration was 50 μ-g/mL, the IL-2, IL-12, IL-4 mRNA expression levels of AA and BC genotype chicken lymphocyte were higher than those of the same genotype. When the traditional Chinese medicines concentration was 100 μg/mL, the IL-2, IL-12, IL-4 mRNA expression levels of BB genotype chicken lymphocyte were higher than those of the same genotype.【Conclusion】The traditional Chinese medicinal polysaccharide extraction could stimulate IL-2, IL-12 , IL-4 mRNA expression in different MHC B-LβII genotype chickens, and the optimum immunomodulatory doses vary in each MHC B-LβII genotype chickens.

Key words: traditional Chinese medicinal polysaccharide; MHC B-LβII gene; chicken lymphocyte; real-time PCR; cytokines

摘要： 【目的】中药复方多糖对不同MHC B-LβII基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响。【方法】采用PCR-SSCP方法将500羽京红1号蛋鸡按不同MHC B-LβII基因型分组,采集不同MHC B-LβII基因型鸡外周血淋巴细胞,分别加入终浓度为100、75、50、0 μg/mL中药复方多糖共培养24 h,收集细胞,采用Real-time PCR方法检测不同 MHC B-LβII基因型鸡IL-2、IL-4、IL-12 基因mRNA的表达量。【结果】(1)与对照组相比,不同剂量中药复方多糖均能显著增强不同 MHC B-LβII基因型鸡IL-2、IL-4、IL-12基因mRNA的表达量(P＜0.05)。(2)中药复方多糖剂量为50 μg/mL时,AA、BC基因型鸡IL-2、IL-4、IL-12基因 mRNA的表达量最高,中药复方多糖剂量为100 μg/mL时,BB基因型鸡IL-2、IL-4、IL-12基因 mRNA的表达量最高。【结论】中药复方多糖能不同程度的促进各MHC B-LβII基因型鸡IL-2、IL-4、IL-12 mRNA的表达,且不同MHC B-LβII基因型鸡淋巴细胞最适中药复方多糖免疫调节剂量不同。

关键词: 中药复方多糖, MHC B-LβII基因, 鸡淋巴细胞, 荧光定量PCR, 细胞因子

CLC Number:

S852

MA Zhao, LIAN Ke-xun, LIU Gang, LIU Xiao-ting, QIAO Yan-jie, ZHU Xiao-qing, GU Xin-li. Effects of Traditional Chinese Medicinal Polysaccharide Extraction on Chicken Lymphocyte of IL-2, IL-4 and IL-12 mRNA Expression in Different MHC B-LβII Genotype Chickens Lymphocyte[J]. Xinjiang Agricultural Sciences, 2017, 54(12): 2320-2328.

马昭, 连科讯, 刘钢, 刘晓婷, 乔严杰, 朱晓庆, 谷新利. 中药复方多糖对不同MHC B-LβII基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响[J]. 新疆农业科学, 2017, 54(12): 2320-2328.

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Effects of Traditional Chinese Medicinal Polysaccharide Extraction on Chicken Lymphocyte of IL-2, IL-4 and IL-12 mRNA Expression in Different MHC B-LβII Genotype Chickens Lymphocyte

中药复方多糖对不同MHC B-LβII基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响

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