Comparative Analysis of Active Ingredients and Selenium Contents  in Astragalus from Different Habitats

doi:10.6048/j.issn.1001-4330.2021.05.011

Xinjiang Agricultural Sciences ›› 2021, Vol. 58 ›› Issue (5): 873-881.DOI: 10.6048/j.issn.1001-4330.2021.05.011

• Horticultural Special Local Produets·Soil Fertilizer·Plant Protection • Previous Articles Next Articles

Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats

JIN Yuanyuan^1,2, MA Shengjun¹, XU Wanli², ZHANG Jun³, GENG Yang¹, WU Wenping¹, WANG Xueshuang¹

1. College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;
2. Institute of Soil Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;
3. Tex Lijianfeng Agroforestry Technology Co. , Ltd,Tekes Xinjiang 835500,China

Received:2020-03-11 Online:2021-05-20 Published:2021-05-26
Correspondence author: MA Shengjun (1977-), male, from Urumqi, Xinjiang, associate professor, Doctor of Medicine, mainly engaged in the research of standardized production and quality control mechanism of Chinese medicinal materials, (E-mail) wlmqmsj @ Sina. com XU Wanli (1971-), male, Baoji, Shaanxi,researcher, Doctor of Agriculture, mainly engaged in soil fertility and improvement research, (E-mail) wlxu2005@ 163.com
Supported by:
Special Demonstration Project for Transformation of Scientific and Technological Achievements in Xinjiang Uygur Autonomous Region "Popularization and Application of Standardized Production Technology of Selenium Rich Agricultural Products" (2019C01020); 2019 China Agricultural University -Xinjiang Agricultural University Scientific Research Cooperation Fund Project "Rhizobium Inoculation Licorice and Astragalus in synergistic Weight Loss Mechanism and Green Planting Technology Demonstration"; 2020 School-Enterprise Cooperation Technology Fund Project "Selenium-Enriched Astragalus Production Key Technology and Product Development Research"

不同产地黄芪活性成分与硒含量比较分析

靳元元^1,2, 马生军¹, 徐万里², 张军³, 耿阳¹, 吴文平¹, 王雪双¹

1.新疆农业大学食品科学与药学学院,乌鲁木齐 830052;
2.新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐 830092;
3.特克斯砺剑锋农林科技有限公司 ,新疆特克斯 835500

通讯作者: 马生军(1977-),男,新疆乌鲁木齐人,副教授,博士,研究方向为中药材规范化生产及质量调控机制,(E-mail)wlmqmsj@sina.com徐万里(1971-),男,陕西宝鸡人,研究员,博士,研究方向为土壤培肥与改良,(E-mail)wlxu2005@163.com
作者简介:靳元元(1994-),女,河北人,硕士研究生,研究方向为中药材规范化生产及质量调控机制,(E-mail)1435380155@qq.com
基金资助:
新疆维吾尔自治区科技成果转化示范专项-科技特派员扶贫行动“富硒农产品标准化生产技术推广应用”(2019C01020); 2019年度中国农业大学-新疆农业大学科研合作基金项目“根瘤菌接种甘草和黄芪的增效减肥机制及绿色化种植技术示范”; 2020年度校企合作技术基金项目“富硒黄芪生产关键技术及产品开发研究”

Abstract

Abstract: 【Objective】 To explore the content of active ingredients and selenium in Astragalus membranaceus from different places and clarify the difference between Astragalus in different places and the correlation between selenium content in Astragalus and active ingredients. 【Methods】 UV spectrophotometry was used to determine the content of total saponins, total flavonoids and total polysaccharides in Astragalus membranaceus, high performance liquid chromatography (HPLC) was applied to determine the content of Calycosin-7-Glucosidein Astragalus membranaceus from different places, and hydride generation-atomic fluorescence method was employed to determine selenium content. After that, statistical analysis of each index was performed. 【Results】 The highest total saponin content of Astragalus in Pinglu district of Shanxi was 1.557%, the highest total flavonoid content of Astragalus in Zhaosu County, Xinjiang was 0.472%, the highest content of Calycosin-7-Glucosidein Astragalus Verticillus in Longde County, Ningxia was 0.091%. Both total polysaccharide content and selenium content were the highest in Bohu County, Xinjiang, which were 27.617% and 0.323 mg/kg, respectively. Correlation analysis showed that the content of selenium and total polysaccharide content were extremely significantly positively correlated (P<0.01). The content differences could be divided into 3 categories. 【Conclusion】 There are significant differences between the active ingredient content of Astragalus and selenium content in 22 different places (P<0.05). The relationship between selenium in Astragalus and total polysaccharide and total saponin content is worthy of more in-depth study, which might provide reference for planting and resource development and utilization.

Key words: Mongolian astragalus; total saponins; total flavonoids; polysaccharides; selenium

摘要： 【目的】研究不同产地黄芪药材中活性成分与硒元素的含量,分析不同产地黄芪之间的差异及黄芪中硒含量与活性成分之间的相关性,为富硒黄芪的种植及资源开发利用提供参考依据。【方法】采用紫外分光光度法测定黄芪总皂苷、总黄酮、总多糖含量,高效液相色谱法(HPLC)测定不同产地黄芪药材中毛蕊异黄酮葡萄糖苷含量,氢化物发生-原子荧光法测定硒的含量,并对各指标进行统计分析。【结果】山西平鲁区黄芪总皂苷含量最高为1.557%,新疆昭苏县黄芪总黄酮含量最高为0.472%,宁夏隆德县黄芪毛蕊异黄酮葡萄糖苷含量最高为0.091%,新疆博湖县总多糖含量和硒含量均是最高,分别为27.617%和0.323 mg/kg;硒含量与总多糖含量呈极显著正相关(P<0.01),聚类分析中黄芪样品可按照不同成分的含量差异分为3类。【结论】22个不同产地黄芪活性成分含量与硒元素的含量有显著差异(P<0.05)。

关键词: 蒙古黄芪, 总皂苷, 总黄酮, 多糖, 硒

CLC Number:

JIN Yuanyuan, MA Shengjun, XU Wanli, ZHANG Jun, GENG Yang, WU Wenping, WANG Xueshuang. Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats[J]. Xinjiang Agricultural Sciences, 2021, 58(5): 873-881.

靳元元, 马生军, 徐万里, 张军, 耿阳, 吴文平, 王雪双. 不同产地黄芪活性成分与硒含量比较分析[J]. 新疆农业科学, 2021, 58(5): 873-881.

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Comparative Analysis of Active Ingredients and Selenium Contents in Astragalus from Different Habitats

不同产地黄芪活性成分与硒含量比较分析

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