Effect on sodium selenite of mycelial growth in Morchella sextelatam of medical edible fungus

doi:10.6048/j.issn.1001-4330.2024.02.027

Abstract

Abstract:

【Objective】 To analyze the effects of different selenium concentrations on Morchella mycelium, and to master the optimal formula of selenium-enriched medium in the hope of providing technical support for the further development of selenium-enriched products.【Methods】 Morchella sextelatam was used as selenium enriched carrier and sodium selenite as selenium source and the effects of different selenium concentrations on the growth trend, mycelial density and mycelial morphology of Morchella sextelatam on different media were systematically analyzed.【Results】 In liquid shake culture, when the concentration of selenium ≤ 20 mg/L, the mycelial morphology no obvious change, and the promoting effect on The mycelial growth of Morchella sextelatam with the increase of concentration, and the dry weight at 20 mg/L.The culture medium, selenium concentration ≤ 20 mg/L had no effect on the mycelial growth, mycelial density and mycelial morphology of Morchella sextelatam.the original mediumthe concentration of selenium ≤ 20 mg/L, there was no significant change in mycelial density and mycelial morphology, and it promoted the growth of Morchella sextelatam, which was the strongest at 10 mg/L.On the cultivation mediumselenium concentration of 20 mg/L the strongest promoting effect on the mycelium of Morchella sextelatam, and no significant change in mycelium density and mycelium morphology.【Conclusion】 Sodium selenite is the best selenium source for Morchella sextelatam cultivation when concentration is 10~20 mg/L.

Key words: Na₂SeO₃; Morchella sextelatam; mycelium; selenium-enrich

摘要：

【目的】分析不同浓度亚硒酸钠对羊肚菌菌丝体在不同培养基上生长的影响,筛选出最佳的培养浓度,掌握多种培养基的最适富硒配方,为开发富硒羊肚菌产品提供技术支撑。【方法】以六妹羊肚菌作为富硒载体,亚硒酸钠为硒源,进行制备试验,通过平板、原种和栽培种制备试验,系统分析在不同培养基上,不同硒浓度对羊肚菌菌丝的生长趋势,菌丝密度,菌丝形态的影响。【结果】在液体摇培中,硒浓度≤20 mg/L,菌丝形态无明显变化,且随浓度升高,对羊肚菌菌丝促生作用增强,且20 mg/L时干重最大;在母种培养基上,硒浓度≤20 mg/L,对羊肚菌的菌丝生长,菌丝密度,菌丝形态均没有影响;在原种培养基上,硒浓度≤20 mg/L,菌丝密度与菌丝形态无明显变化,且对羊肚菌的菌丝有促生作用,在10 mg/L时最强;在栽培种培养基上,硒浓度20 mg/L,对羊肚菌菌丝体的促生作用最强,菌丝密度与菌丝形态均无明显变化。【结论】以亚硒酸钠作为硒源,硒浓度在10~20 mg/L效果最好,可作为羊肚菌富硒栽培较为理想的浓度。

关键词: 亚硒酸钠, 六妹羊肚菌, 菌丝体, 富硒

CLC Number:

S567.3

LI Mengtong, Mayila , YU Lyujian, DING Yu, YANG Jin, JIAO Ziwei. Effect on sodium selenite of mycelial growth in Morchella sextelatam of medical edible fungus[J]. Xinjiang Agricultural Sciences, 2024, 61(2): 495-504.

李梦桐, 玛依拉, 于吕健, 丁宇, 阳瑾, 焦子伟. Na₂SeO₃对药食用真菌羊肚菌菌丝体生长的影响[J]. 新疆农业科学, 2024, 61(2): 495-504.

Figures/Tables 16

References 18

[1]	张迪, 何娜, 杨晓莉, 等. 硒蛋白对人体健康重要作用的研究进展[J]. 科学通报, 2022, 67(6):473-480.
	ZHANG Di, HE Na, YANG Xiaoli, et al. Research advance on the important role of selenoprotein in human health[J]. Chinese Science Bulletin, 2022, 67(6):473-480.
[2]	王磊, 杜菲, 孙卉, 等. 人体硒代谢与硒营养研究进展[J]. 生物技术进展, 2015, 5(4):285-290. DOI
	WANG Lei, DU Fei, SUN Hui, et al. Progress on Selenium Metabolism and Nutrition in Human[J]. Current Biotechnology, 2015, 5(4):285-290. DOI
[3]	李傲瑞, 乔新星, 赵飞飞, 等. 硒与人体健康关系研究进展[J]. 绿色科技, 2020,(12):121-122.
	LI Aorui, QIAO Xinxing, ZHAO Feifei, et al. Research Progress on the Relationship Between Selenium and Human Health[J]. Journal of Green Science and Technology, 2020,(12):121-122.
[4]	王福俤. 中国生物微量元素研究的现状与展望[J]. 生命科学, 2012, 24(8):713-730.
	WANG Fudi. Trace element research in China:Present and future[J]. Chinese Bulletin of Life Sciences, 2012, 24(8):713-730.
[5]	倪淑君, 张海峰. 我国羊肚菌的产业发展[J]. 北方园艺, 2019,(2):165-167.
	NI Shujun, ZHANG Haifeng. Industry Development of Morchella in China[J]. Northern Horticulture, 2019,(2):165-167.
[6]	向戌莲. 羊肚菌的集硒特性及含硒活性物质初步研究[D]. 恩施: 湖北民族大学, 2020.
	XIANG Xulian. Preliminary study on selenium collecting characteristics and active substances of Morchella esculenta[D]. Enshi: Hubei Minzu University, 2020.
[7]	梁克红, 卢林纲, 朱宏, 等. 食物中硒的研究进展[J]. 中国粮油学报, 2018, 33(3):134-138.
	LIANG Kehong, LU Lingang, ZHU Hong, et al. Research Progress of Selenium in Food[J]. Journal of the Chinese Cereals and Oils Association, 2018, 33(3):134-138.
[8]	朱燕云, 吴文良, 赵桂慎, 等. 硒在动植物及微生物体中的转化规律研究进展[J]. 农业资源与环境学报, 2018, 35(3):189-198.
	ZHU Yanyun, WU Wenliang, ZHAO Guishen, et al. Progress of Selenium Biological Transformation in Animals, Plants, and Microorganisms[J]. Journal of Agricultural Resources and Environment, 2018, 35(3):189-198.
[9]	杨善岩, 李海龙, 狄志鸿. 硒元素生理功能及微生物富硒发酵研究现状[J]. 食品工业, 2013, 34(6):167-170.
	YANG Shanyan, LI Hailong, DI Zhihong. Research Status of the Physiological Functions of Selenium and Concentrating Selenium of Microbe[J]. The Food Industry, 2013, 34(6):167-170.
[10]	胡婷, 惠改芳, 赵桂慎, 等. 富硒食用菌研究进展[J]. 食用菌学报, 2019, 26(1):68-76.
	HU Ting, HUI Gaifang, ZHAO Guishen, et al. Advances in Selenium-enriched Edible Fungi[J]. Acta Edulis Fungi, 2019, 26(1):68-76.
[11]	赵蕾. 灵芝生物富硒及富硒灵芝硒蛋白的分离纯化和抗氧化性研究[D]. 北京: 中国农业大学, 2004.
	ZHAO Lei. Biotransformation of selenium,purification of seleno-protein and antioxidant activity of se-enriched Ganoderma lucidum[D]. Beijing: China Agricultural University, 2004.
[12]	胡志斌, 甘泉, 魏赛金, 等. 灵芝液体发酵富集硒元素研究[J]. 中国酿造, 2010, 20(4):36-39.
	HU Zhibin, GAN Quan, WEI Saijin, et al. Selenium dosage for submerged culturing enriched Ganoderma lucidum[J]. China Brewing, 2010, 20(4):36-39.
[13]	刘书畅, 马布平, 周忠发, 等. 富硒食用菌的研究进展(综述)[J]. 食药用菌, 2018, 26(2):74-78.
	LIU Shuchang, MA Buping, ZHOU Zhongfa, et al. Research progress of selenium-enriched edible mushrooms[J]. Edible and Medicinal Mushrooms, 2018, 26(2):74-78.
[14]	吕明帅, 赵博, 孙文玉, 等. 富硒黑木耳多糖的理化性质及抗氧化活性研究[J]. 中国调味品, 2021, 46(6):54-59.
	LYU Mingshuai, ZHAO Bo, SUN Wenyu, et al. Research on Physicochemical Properties and Antioxidant Activity[J]. China Condiment, 2021, 46(6):54-59.
[15]	努尔买买提, 焦子伟, 杨晓绒, 等. 不同人工栽培培养基对蛹虫草子实体发育的影响及效益分析[J]. 新疆农业科学, 2016, 53(2):359-366.
	Nurmaimaiti, JIAO Ziwei, YANG Xiaorong, et al. Different cultivated medium on Cordyceps Militaris fruit body development impact and benefit analysis[J]. Xinjiang Agricultural Sciences, 2016, 53(2):359-366.
[16]	刘伟, 张亚, 何培新. 羊肚菌生物学与栽培技术[M]. 长春: 吉林科学技术出版社, 2017.
	LIU Wei, ZHANG Ya, HE Peixin. Morel Bioiogy and Cultivation[M]. Changchun: Jilin Science&Technology Publishing House, 2017.
[17]	徐联鹏, 吴萍, 陆晨晖, 等. 富硒秀珍菇的培育及其营养品质评价[J]. 南京晓庄学院学报, 2021, 37(6):53-58.
	XU Lianpeng, WU Ping, LU Chenghui, et al. Cultivation of Selenium-Rich Pleurotus Geesteranus and Evaluation of Nutrition Quality[J]. Journal of Nanjing Xiaozhuang University, 2021, 37(6):53-58.
[18]	李发盛, 黄雪星, 欧娜, 等. 云耳菌株“百云6号”菌丝体对外源硒的耐受性研究[J]. 中国食用菌, 2021, 40(7):52-57.
	LI Fasheng, HUANG Xuexing, OU Na, et al. Study on the Tolerance of Exogenous Selenium in Mycelium of Auricularia auricula-judae ‘Baiyun No.6’[J]. Edible Fungi of China, 2021, 40(7):52-57.

编号 Number	培养基 Medium	培养基配方 Medium ingredients
1	PD加富培养基	马铃薯200 g、葡萄糖20 g、麸皮30 g、水1 000 mL、pH自然
2	PDA加富培养基	马铃薯200 g、葡萄糖20 g、琼脂20 g、麸皮30 g、水1 000 mL、pH自然
3	原种培养基	木屑70%,麸皮10%,麦子10%, 腐殖土10%
4	栽培种培养基	木屑57%,小麦33%,腐殖土10%

编号 Number	培养基 Medium	培养基配方 Medium ingredients
1	PD加富培养基	马铃薯200 g、葡萄糖20 g、麸皮30 g、水1 000 mL、pH自然
2	PDA加富培养基	马铃薯200 g、葡萄糖20 g、琼脂20 g、麸皮30 g、水1 000 mL、pH自然
3	原种培养基	木屑70%,麸皮10%,麦子10%, 腐殖土10%
4	栽培种培养基	木屑57%,小麦33%,腐殖土10%

编号 Number	培养基 Medium	硒浓度 Selenium concentration(mg/L)
1	PD加富培养基	0、10、20、40、60
2	PDA加富培养基	0、5、10、20、40、60、80、100、150、200
3	原种培养基	0、10、20、40、60、80、100、150、200
4	栽培种培养基	0、10、20、40、60、80、100、150、200

编号 Number	培养基 Medium	硒浓度 Selenium concentration(mg/L)
1	PD加富培养基	0、10、20、40、60
2	PDA加富培养基	0、5、10、20、40、60、80、100、150、200
3	原种培养基	0、10、20、40、60、80、100、150、200
4	栽培种培养基	0、10、20、40、60、80、100、150、200

处理 Treatments	硒浓度 Selenium concentration(mg/L)	菌丝干重 Dry weight of mycelium(g)
CK	0	0.698 3±0.004 4^a
1	10	0.715 3±0.004 8^a
2	20	0.734 6±0.006 5^a
3	40	0.511 3±0.024 5^b
4	60	0.475 7±0.008 7^b