Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (2): 495-504.DOI: 10.6048/j.issn.1001-4330.2024.02.027

• Microbes·Physiology and Biochemistry·Animal Husbandry Veterinarian • Previous Articles     Next Articles

Effect on sodium selenite of mycelial growth in Morchella sextelatam of medical edible fungus

LI Mengtong(), Mayila , YU Lyujian, DING Yu, YANG Jin, JIAO Ziwei()   

  1. College of Biological and Geographical Sciences, Yili Normal University, Yining Xinjiang 835000,China
  • Received:2023-07-10 Online:2024-02-20 Published:2024-03-19
  • Correspondence author: JIAO Ziwei (1973-), male, from Huaiyang,Henan Ph.D., professor, research for microbial ecology,(E-mail)741285332@qq.com
  • Supported by:
    The Opening Project for Key Laboratory of Microbial Resources Protection, Development and Utilization(YLUKLM202001);Key research and technology development projects in Ili Kazakh Autonomous Prefecture(YZ2023A05)

Na2SeO3对药食用真菌羊肚菌菌丝体生长的影响

李梦桐(), 玛依拉, 于吕健, 丁宇, 阳瑾, 焦子伟()   

  1. 伊犁师范大学生物与地理科学学院,新疆伊宁 835000
  • 通讯作者: 焦子伟(1973-),男,河南淮阳人,教授,博士,博士生导师,研究方向为微生物生态,(E-mail)741285332@qq.com
  • 作者简介:李梦桐(1993-),女,河南新乡人,硕士研究生,研究方向为羊肚菌栽培,(E-mail)271200810@qq.com
  • 基金资助:
    微生物资源保护与开发利用重点实验室开放项目(YLUKLM202001);伊犁州重点研究与技术开发专项项目(YZ2023A05)

Abstract:

【Objective】 To analyze the effects of different selenium concentrations on Morchella mycelium, and to master the optimal formula of selenium-enriched medium in the hope of providing technical support for the further development of selenium-enriched products.【Methods】 Morchella sextelatam was used as selenium enriched carrier and sodium selenite as selenium source and the effects of different selenium concentrations on the growth trend, mycelial density and mycelial morphology of Morchella sextelatam on different media were systematically analyzed.【Results】 In liquid shake culture, when the concentration of selenium ≤ 20 mg/L, the mycelial morphology no obvious change, and the promoting effect on The mycelial growth of Morchella sextelatam with the increase of concentration, and the dry weight at 20 mg/L.The culture medium, selenium concentration ≤ 20 mg/L had no effect on the mycelial growth, mycelial density and mycelial morphology of Morchella sextelatam.the original mediumthe concentration of selenium ≤ 20 mg/L, there was no significant change in mycelial density and mycelial morphology, and it promoted the growth of Morchella sextelatam, which was the strongest at 10 mg/L.On the cultivation mediumselenium concentration of 20 mg/L the strongest promoting effect on the mycelium of Morchella sextelatam, and no significant change in mycelium density and mycelium morphology.【Conclusion】 Sodium selenite is the best selenium source for Morchella sextelatam cultivation when concentration is 10~20 mg/L.

Key words: Na2SeO3; Morchella sextelatam; mycelium; selenium-enrich

摘要:

【目的】分析不同浓度亚硒酸钠对羊肚菌菌丝体在不同培养基上生长的影响,筛选出最佳的培养浓度,掌握多种培养基的最适富硒配方,为开发富硒羊肚菌产品提供技术支撑。【方法】以六妹羊肚菌作为富硒载体,亚硒酸钠为硒源,进行制备试验,通过平板、原种和栽培种制备试验,系统分析在不同培养基上,不同硒浓度对羊肚菌菌丝的生长趋势,菌丝密度,菌丝形态的影响。【结果】在液体摇培中,硒浓度≤20 mg/L,菌丝形态无明显变化,且随浓度升高,对羊肚菌菌丝促生作用增强,且20 mg/L时干重最大;在母种培养基上,硒浓度≤20 mg/L,对羊肚菌的菌丝生长,菌丝密度,菌丝形态均没有影响;在原种培养基上,硒浓度≤20 mg/L,菌丝密度与菌丝形态无明显变化,且对羊肚菌的菌丝有促生作用,在10 mg/L时最强;在栽培种培养基上,硒浓度20 mg/L,对羊肚菌菌丝体的促生作用最强,菌丝密度与菌丝形态均无明显变化。【结论】以亚硒酸钠作为硒源,硒浓度在10~20 mg/L效果最好,可作为羊肚菌富硒栽培较为理想的浓度。

关键词: 亚硒酸钠, 六妹羊肚菌, 菌丝体, 富硒

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