Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

doi:10.6048/j.issn.1001-4330.2024.03.006

Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 576-583.DOI: 10.6048/j.issn.1001-4330.2024.03.006

• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Physiology and Biochemistry • Previous Articles Next Articles

Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

CHEN Pei¹(), BAO Junxiu¹, WANG Fei¹^,²^,³, TANG Shouwu⁴, LIU Haifeng⁴(), LI Hongbin¹^,²^,³^,⁴()

1. College of Life Sciences,Shihezi University,Shihezi Xinjiang 832003,China
2. Key Laboratory of Oasis Town and Mountain Basin System Ecology,Xinjiang Production and Construction Corps,Shihezi Xinjiang 832000,China
3. Key Laboratory of Xinjiang Phytomedicine Resource and Utilization,Ministry of Education,Shihezi Xinjiang 832000,China
4. China Natural Color Cotton Corp.Ltd.,Urumqi 830001,China

Received:2023-08-03 Online:2024-03-20 Published:2024-04-19
Correspondence author: LI Hongbin(1980-),male,from Henan,professor,Ph.D.,doctoral supervisor,research field:plant molecular biology,(E-mail) lihb@shzu.edu.cn;LIU Haifeng,(1977-),male,from Hubei,master supervisor,research field:cotton genetics and breeding,(E-mail) cncclhf@163.com.
Supported by:
Innovation Environment Construction Project of Xinjiang Uygur Autonomous Region(2020D14035);Tackling Hard-Nut Problems in Science and Technology and Research Results Transformation Project of XPCC(2016AC017)

响应面优化超声提取绿色棉纤维总黄酮

陈沛¹(), 鲍军秀¹, 王斐¹^,²^,³, 汤寿伍⁴, 刘海峰⁴(), 李鸿彬¹^,²^,³^,⁴()

1.石河子大学生命科学学院,新疆石河子 832003
2.绿洲城镇与山盆系统生态兵团重点实验室,新疆石河子 832000
3.新疆植物药资源利用教育部重点实验室,新疆石河子 832000
4.中国彩棉(集团)股份有限公司,乌鲁木齐 830001

通讯作者: 李鸿彬(1980-),男,河南人,教授,博士,硕士生/博士生导师,研究方向为植物分子生物学,(E-mail)lihb@shzu.edu.cn;刘海峰(1977-),男,湖北人,研究员,硕士生导师,研究方向为棉花遗传育种,(E-mail)cncclhf@163.com
作者简介:陈沛(1996-),男,四川人,硕士研究生,研究方向为植物分子生物学,(E-mail)554538275@qq.com
基金资助:
新疆维吾尔自治区创新环境建设专项(2020D14035);新疆生产建设兵团科技攻关与成果转化项目(2016AC017)

Abstract

Abstract:

【Objective】 This study is to optimize ultrasonic extraction conditions of total flavonoids in NGC fibers,in the hope of providing the basis for reasonable utilization and identification of NGC fibers.【Methods】 The NGC fibers were used as raw materials and were utilized for total flavonoids extract by ultrasonic cavitation effect to accelerate the dissolution of flavonoids in ethanol,to optimize the experimental conditions with Box-Benhnken center combination principle and response surface design.【Results】 The optimal extraction conditions were as follows:solid-liquid ratio of 1∶50 g/mL,ethanol concentration of 50%,and extraction time of 50 min.Under these conditions,the average yield of total flavonoids was 5.25%,which was the best condition for the extraction of total flavonoids from NGC fibers by ultrasonic method.【Conclusion】 The optimal condition Parameter for ultrasonic extraction of total flavonoids from green cotton fibers is a liquid to material ratio of 1∶50 g/mL,ethanol concentration 50%,extraction time 50 min,obtaining an average total flavonoid yield of 5.25%.

Key words: green cotton fiber; total flavonoids; response surface method; optimum extraction condition; ultrasionic cell-break

摘要：

【目的】 优化超声提取绿色棉纤维中总黄酮工艺条件,为合理利用及鉴别天然绿色棉纤维提供依据。【方法】 以天然绿色棉纤维为原料,利用超声空化效应加速黄酮溶解于乙醇中,在单因素试验基础上,结合Box-Benhnken中心组合原理及响应面设计优化试验条件。【结果】 料液比为1∶50 g/mL时,总黄酮得率达到最大值,提取最适料液比为1∶50 g/mL,响应面试验中建立的模型的CV值=0.87%,影响绿色棉纤维总黄酮得率的顺序为提取时间>乙醇浓度>料液比,A料液比为1∶50 g/mL、B乙醇浓度50%、C提取时间50 min为最佳提取条件。【结论】 超声波法提取绿色棉纤维总黄酮的最佳条件参数为料液比1∶50 g/mL、乙醇浓度50%、提取时间50 min;获得平均总黄酮得率为5.25%。

关键词: 绿色棉纤维, 总黄酮, 响应面法, 最佳提取条件, 超声波细胞破碎

CLC Number:

S122

CHEN Pei, BAO Junxiu, WANG Fei, TANG Shouwu, LIU Haifeng, LI Hongbin. Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization[J]. Xinjiang Agricultural Sciences, 2024, 61(3): 576-583.

陈沛, 鲍军秀, 王斐, 汤寿伍, 刘海峰, 李鸿彬. 响应面优化超声提取绿色棉纤维总黄酮[J]. 新疆农业科学, 2024, 61(3): 576-583.

Figures/Tables 7

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因素 Factor	水平Level
因素 Factor	1	2	3	4	5
料液比 Solid-liquid ratio(g/mL)	1∶30	1∶40	1∶50	1∶60	1∶70
乙醇浓度 Ethanol concentration(%)	30	40	50	60	70
提取功率比 Extraction power ratio(%)	30	40	50	60	70
提取时间 Extraction time(min)	30	40	50	60	70

因素 Factor	水平Level
因素 Factor	1	2	3	4	5
料液比 Solid-liquid ratio(g/mL)	1∶30	1∶40	1∶50	1∶60	1∶70
乙醇浓度 Ethanol concentration(%)	30	40	50	60	70
提取功率比 Extraction power ratio(%)	30	40	50	60	70
提取时间 Extraction time(min)	30	40	50	60	70

试验序号 Experiment number	因素代码及编码 Factor code and coding			总黄酮得率 Total flavonoid yield(%)
试验序号 Experiment number	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentration	C.提取时间 C.Extraction time	总黄酮得率 Total flavonoid yield(%)
1	1	1	0	3.77
2	0	0	0	5.29
3	0	1	-1	4.07
4	-1	0	-1	4.01
5	1	-1	0	3.91
6	1	0	-1	4.13
7	0	0	0	5.26
8	0	-1	-1	4.02
9	0	0	0	5.21
10	0	0	0	5.27
11	1	0	1	4.13
12	0	-1	1	4.18
13	-1	-1	0	3.85
14	0	1	1	4.11
15	0	0	0	5.31
16	-1	0	1	4.15
17	-1	1	0	3.89

试验序号 Experiment number	因素代码及编码 Factor code and coding			总黄酮得率 Total flavonoid yield(%)
试验序号 Experiment number	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentration	C.提取时间 C.Extraction time	总黄酮得率 Total flavonoid yield(%)
1	1	1	0	3.77
2	0	0	0	5.29
3	0	1	-1	4.07
4	-1	0	-1	4.01
5	1	-1	0	3.91
6	1	0	-1	4.13
7	0	0	0	5.26
8	0	-1	-1	4.02
9	0	0	0	5.21
10	0	0	0	5.27
11	1	0	1	4.13
12	0	-1	1	4.18
13	-1	-1	0	3.85
14	0	1	1	4.11
15	0	0	0	5.31
16	-1	0	1	4.15
17	-1	1	0	3.89

方差来源 Source of variation	平方和 Quadratic sum	自由度 Degree of freedom	均方 Mean square	F值 F value	P值 P-value
模型Model	5.71	9	0.63	438.03	< 0.000 1
A	2.00E-04	1	2.00E-04	0.14	0.7211
B	1.80E-03	1	1.80E-03	1.24	0.301 6
C	0.014	1	0.014	9.99	0.015 9
AB	8.10E-03	1	8.10E-03	5.6	0.049 9
AC	4.90E-03	1	4.90E-03	3.39	0.108 3
BC	3.60E-03	1	3.60E-03	2.49	0.158 7
A²	2.07	1	2.07	1 431.79	< 0.000 1
B²	2.13	1	2.13	1 472.91	< 0.000 1
C²	0.9	1	0.9	619.68	< 0.000 1
失拟项Lack of fit term	4.45E-03	3	1.48E-03	1.04	0.464
误差项Error term	5.68E-03	4	1.42E-03
总和Summation	5.72	16
R²=0.998 2		R^2Adj=0.995 9		CV=0.87%

Extraction of total flavonoids from green cotton fiber by ultrasonic-based response surface optimization

响应面优化超声提取绿色棉纤维总黄酮

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因素 Factor	编码水平Coding level
因素 Factor	-1	0	1
A.料液比 A.Solid-liquid ratio(g/mL)	1∶40	1∶50	1∶60
B.乙醇浓度 B.Ethanol concentration(%)	40	50	60
C提取时间 C:Extraction time(min)	40	50	60

项目 Items	A.料液比 A.Solid- liquid ratio	B.乙醇浓度 B.Ethanol concentr ation	C.提取时间 C.Extra ction time	总黄酮得率 Total flavonoid yield(%)
编码值 Coding value	0.000	-0.011	0.047	5.269
最佳条件 Optimum condition	1∶50 g/mL	50%	50 min	5.25

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