Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

doi:10.6048/j.issn.1001-4330.2018.11.004

Xinjiang Agricultural Sciences ›› 2018, Vol. 55 ›› Issue (11): 1988-1993.DOI: 10.6048/j.issn.1001-4330.2018.11.004

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Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

WANG Zhong^1,2, FAN Zhe-ru^1,2, ZHANG Yue-qiang^1,2, LI Jian-feng^1,2, GAO Xin^1,2, WANG Zi-xia¹

1.Research Institute of Nuclear and Biological Technologies, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
2. Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region, Ministry of Agriculture, P.R. China, Urumqi 830091, China

Received:2018-09-15 Published:2019-02-22
Correspondence author: FAN Zhe-ru(1964-),male, Minqin of Gansu Province, Researcher, Research focuses on wheat genetics and breeding, E-mail:fzr518@163.com
Supported by:
Youth Science Fund Projects of Xinjiang Uygur Autonomous Region (2013211B23); Youth Science Fund Projects of Xinjiang Academy of Agricultural Sciences(xjnkq-2013025)

不同培养基对春小麦×玉米单倍体胚分化成苗的影响

王重^1,2, 樊哲儒^1,2, 张跃强^1,2, 李剑峰^1,2, 高新^1,2, 王子霞¹

1.新疆农业科学院核技术生物技术研究所,乌鲁木齐 830091;
2.农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐 830091

通讯作者: 樊哲儒(1964-),男,甘肃民勤人,研究员,研究方向为小麦遗传育种,E-mail:fzr518@163.com
作者简介:王重(1982-),男,陕西长安人,副研究员,研究方向为小麦遗传育种,E-mail:wangzhongac@126.com
基金资助:
新疆维吾尔自治区青年科学基金项目(2013211B23);新疆农业科学院青年科学基金项目(xjnkq-2013025)

Abstract

Abstract: 【Objective】 This project aims to screen suitable medium for differentiation of spring wheat haploid embryos and obtain a stable and efficient method.【Method】The haploid embryos were differentiated into plantlets using 8 different mediums,and the mediums was screened according to the seedling rate and growth conditions. 【Result】The seedling rate of spring wheat haploid embryos was the highest (73.49%) in 1/2 MS medium without hormones. The haploid embryos were induced one-step-seedling in 1/2 MS + 0.1 mg/L 2,4- D medium, with stronger plantlets and their roots developed well. 【Conclusion】The 1/2 MS medium was more suitable for the differentiation of spring wheat haploid embryos than the B5 medium, and the 2,4-D had more significant effect on the development of roots and differentiation of embryos. The medium of 1/2 MS + 0.1 mg/L 2, 4-D was a suitable medium for haploid embryos differentiation of spring wheat.

Key words: wheat; haploid embryo; differentiation; medium

摘要： 【目的】筛选适宜春小麦单倍体胚分化成苗的培养基,获得稳定且高效的单倍体胚培养方法。【方法】分别使用8种不同配方培养基对春小麦单倍体胚进行离体培养,使其分化成苗,根据成苗率及生长情况筛选培养基。【结果】在不添加激素的1/2 MS培养基中春小麦单倍体胚成苗率最高(73.49%),在1/2MS+0.1 mg/L 2,4-D培养基中春小麦单倍体胚能够一步成苗,且生长健壮,根系发达。【结论】1/2 MS培养基较B5培养基更适宜春小麦单倍体胚的分化成苗,2,4-D对于春小麦单倍体胚的根系发育具有明显的促进作用,2,4-D>IAA,1/2 MS+0.1 mg/L 2,4-D培养基是春小麦单倍体胚分化成苗的适宜培养基。

关键词: 春小麦, 单倍体胚, 分化, 培养基

CLC Number:

S512

WANG Zhong, FAN Zhe-ru, ZHANG Yue-qiang, LI Jian-feng, GAO Xin, WANG Zi-xia. Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross[J]. Xinjiang Agricultural Sciences, 2018, 55(11): 1988-1993.

王重, 樊哲儒, 张跃强, 李剑峰, 高新, 王子霞. 不同培养基对春小麦×玉米单倍体胚分化成苗的影响[J]. 新疆农业科学, 2018, 55(11): 1988-1993.

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Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

不同培养基对春小麦×玉米单倍体胚分化成苗的影响

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