Effects of microbial inoculants on physiological and photosynthetic characteristics of walnut leaves

doi:10.6048/j.issn.1001-4330.2024.09.026

Abstract

Abstract:

【Objective】 To explore the effects of microbial microbial agents on the physiological and photosynthetic characteristics of walnut leaves,and to provide scientific basis for the application of microbial microbial agents on walnut in Aksu area. 【Methods】 A total of 6 experimental treatments were set up with 14-year old walnut "Wen185" as the test material,which were 30 g/ strain of Bacillus subtilis (T₁), 30 g/ strain of Trichoderma hartzii (T₂), 40 g/ strain of Bacillus subtilis (T₃), 40 g/ strain of Trichoderma hartzii (T₄), and 30 g/ strain of Trichoderma Hartzii. Bacillus subtilis 20 g/ strain + Trichoderma harziensis 20 g/ strain (T₅), water control (CK). Then, leaf area, leaf length, leaf width, fresh weight and dry weight were measured. After thatn, chlorophyll (SPAD), photosynthetic characteristics and other indicators were determined and the effects of different amounts of microbial inoculants on walnut leaves were analyzed. 【Results】 Under T5 treatment, the area, length, fresh weight and dry weight of walnut leaves were significantly higher than those of control, which were 31.75%,11.55%,90.83% and 166.66% higher, respectively. Leaf nutrient contents of nitrogen, phosphorus and potassium showed the maximum value, which was 65.68%,61.14% and 57.47% higher than that of the control. Chlorophyll content and photosynthetic characteristics index parameters were also increased under this treatment. 【Conclusion】 Trench application of 20 g on each of Bacillus subtilis and Trichoderma harziana promote the growth,nutrient content and photosynthetic performance of walnut leaves. In this experiment, the combination of Bacillus subtilis 20 g and Trichoderma harziana 20 g has the best effect.

Key words: walnut; microbial agent; blades; photosynthetic characteristics

摘要：

【目的】研究微生物菌剂对核桃叶片生理及光合特性影响,为微生物菌剂在新疆阿克苏地区核桃上应用提供科学依据。【方法】以14年生疏散分层形温185核桃为材料,共设置6个处理,分别为枯草芽孢杆菌30 g/株(T₁)、哈茨木霉菌30 g/株(T₂)、枯草芽孢杆菌40 g/株(T₃)、哈茨木霉菌40 g/株(T₄)、枯草芽孢杆菌20 g/株+哈茨木霉菌20 g/株(T₅)和清水对照(CK)。测定核桃叶片叶面积、叶长、叶宽、鲜重、干重、叶绿素(SPAD)、光合特征等指标,分析不同用量及种类的微生物菌剂对核桃叶片的影响。【结果】在T₅处理下核桃叶片生长指标表现最优,T₅处理下叶片养分含量氮、磷、钾出现最大值,较对照高出65.68%,61.14%和57.47%;该处理下叶片叶绿素含量与CK呈显著差异,较对照高29.38%,且提高了叶片净光合速率、气孔导度、蒸腾速率,较对照分别高22.57%、21.16%和65.58%。【结论】沟施枯草芽孢杆菌和哈茨木霉菌各20 g处理促进核桃叶片生长及养分含量和提高光合性能的效果最佳。

关键词: 核桃, 微生物菌剂, 叶片, 光合特性

CLC Number:

S664.1
S14

Paziliye Ahemati, WANG Xinyong, ZHOU Yan, SONG Bin, Yusuf Abulitifu. Effects of microbial inoculants on physiological and photosynthetic characteristics of walnut leaves[J]. Xinjiang Agricultural Sciences, 2024, 61(9): 2299-2306.

帕孜丽耶·艾合麦提, 王新勇, 周燕, 宋彬, 玉苏甫·阿不力提甫. 微生物菌剂对核桃叶片生理及光合特性的影响[J]. 新疆农业科学, 2024, 61(9): 2299-2306.

Figures/Tables 11

References 23

[1]	郗荣庭, 张毅萍. 中国果树志-核桃卷[M]. 北京: 中国林业出版社, 1996: 1-158.
	XI Rongting, ZHANG Yipin. China fruit Zhi-Walnut Roll[M]. Beijing: China Forestry Publishing House, 1996: 1-158.
[2]	席婧, 蒋志辉. 新疆地区核桃产业发展现状分析[J]. 现代园艺, 2023, 46(2): 38-40.
	XI Jing, JIANG Zhihui. Analysis on the development status of walnut industry in Xinjiang[J]. Contemporary Horticulture, 2023, 46(2): 38-40.
[3]	Gond S K, Bergen M S, Torres M S, et al. Endophytic Bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize[J]. Microbiological Research, 2015, 172: 79-87.
[4]	Gagné-Bourque F, Bertrand A, Claessens A, et al. Alleviation of drought stress and metabolic changes in timothy (Phleum pratense L.) colonized with Bacillus subtilis B26[J]. Frontiers in Plant Science, 2016, 7: 584.
[5]	Zhang F G, Yuan J, Yang X M, et al. Putative Trichoderma harzianum mutant promotes cucumber growth by enhanced production of indole acetic acid and plant colonization[J]. Plant and Soil, 2013, 368(1): 433-444.
[6]	辛磊, 安慧, 钱丰, 等. 枯草芽孢杆菌ML-11对核桃根腐病的防治效果研究[J]. 农业技术与装备, 2022, (7): 5-6,8.
	XIN Lei, AN Hui, QIAN Feng, et al. Study on the control effect of Bacillus subtilis ML-11 on walnut root rot[J]. Agricultural Technology and Equipment, 2022, (7): 5-6,8.
[7]	周晓馥, 张欣玥, 蔡汶妤, 等. 哈茨木霉对黄瓜幼苗促生作用的影响[J]. 吉林师范大学学报(自然科学版), 2020, 41(3): 93-99.
	ZHOU Xiaofu, ZHANG Xinyue, CAI Wenyu, et al. Study on effects of Trichoderma Harzianum on growth-promotion of cucumber seedlings[J]. Journal of Jilin Normal University (Natural Science Edition), 2020, 41(3): 93-99.
[8]	张祖衔, 邓薇, 李春, 等. 施加枯草芽孢杆菌和哈茨木霉对黄瓜幼苗生长的影响[J]. 北方园艺, 2021, (23): 11-20.
	ZHANG Zuxian, DENG Wei, LI Chun, et al. Effects of Bacillus subtilis and Trichoderma harzianum on the growth of cucumber seedlings[J]. Northern Horticulture, 2021, (23): 11-20.
[9]	周进. 生物菌肥施用对温室辣椒光合特性、产量和品质的影响[J]. 北方园艺, 2021, (1): 42-47.
	ZHOU Jin. Effects of microbial fertilizer application on photosynthetic characteristics, yield and quality of greenhouse pepper[J]. Northern Horticulture, 2021, (1): 42-47.
[10]	王爱玲, 段国琪, 田时敏, 等. 减氮配施微生物菌肥对 "富士" 苹果品质和光合特性的影响[J]. 北方园艺, 2022, (18): 16-22.
	WANG Ailing, DUAN Guoqi, TIAN Shimin, et al. Effects of combined application of microbial fertilizer on quality and photosynthetic characteristics of'Fuji'Apple[J]. Northern Horticulture, 2022, (18): 16-22.
[11]	杨皓, 庄家尧, 郑康, 等. 不同载体菌肥对刺槐光合特性及土壤养分、细菌群落的影响[J]. 核农学报, 2023, 37(4):844-853.
	YANG Hao, ZHUANG Jiayao, ZHENG Kang, et al. Effects of different carrier bacterial fertilizers on the photosynthetic characteristics, soil nutrients and bacterial communities of black locust[J]. Journal of Nuclear Agricultural Sciences, 2023, 37(4): 844-853.
[12]	侯彦林, 任军. 生态平衡施肥技术产业化模式和机制研究[J]. 土壤通报, 2003, 34(3): 191-194.
	HOU Yanlin, REN Jun. Studies on industrialization mode and mechanism of ecological balanced fertilization technique[J]. Chinese Journal of Soil Science, 2003, 34(3): 191-194.
[13]	贺志斌. 微生物肥料在苹果种植中的应用效果研究[D]. 临汾: 山西师范大学, 2018.
	HE Zhibin. Application Effect of Microbial Fertilizer on Apple Planting[D]. Linfen: Shanxi Normal University, 2018.
[14]	段淇斌, 赵冬青, 姚拓, 等. 施用生物菌肥对饲用玉米生长和土壤微生物数量的影响[J]. 草原与草坪, 2015, 35(2): 54-58.
	DUAN Qibin, ZHAO Dongqing, YAO Tuo, et al. Effects of usingbiofertilizer on forage maize growth and soil microbial number[J]. Grassland and Turf, 2015, 35(2): 54-58.
[15]	穆凯代斯罕·伊萨克. HPS微生物菌肥对库尔勒香梨园土壤、叶片和果实品质的影响研究[D]. 塔里木大学, 2023.
	Mukaidaisihan Yisake. Research on the effects of HPS microbial fertilizer on soil, leaves and fruit quality of Korla fragrant pear orchards[D]. Aral: Tarim University, 2023.
[16]	冯大兰, 魏立本, 黄小辉, 等. 梁平柚果实膨大期叶片矿质营养诊断研究[J]. 南京林业大学学报(自然科学版), 2020, 44(2): 111-116.
	FENG Dalan, WEI Liben, HUANG Xiaohui, et al. Diagnostic study on mineral nutrition in leaves of Liangping pomelo during fruit expansion period[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2020, 44(2): 111-116.
[17]	付燕, 杨芩. 不同浓度食用醋对蓝莓生长势及叶片N·P·K含量的影响[J]. 安徽农业科学, 2017, 45(18): 24-26.
	FU Yan, YANG Qin. Effect of different concentrations of vinegar on growth potential and nitrogen, phosphorus and potassium content in leaves of blueberry[J]. Journal of Anhui Agricultural Sciences, 2017, 45(18): 24-26.
[18]	钟全林, 程栋梁, 胡松竹, 等. 刨花楠和华东润楠叶绿素含量分异特征及与净光合速率的关系[J]. 应用生态学报, 2009, 20(2): 271-276.
	ZHONG Quanlin, CHENG Dongliang, HU Songzhu, et al. Chlorophyll content and net photosynthetic rate of Machilus pauhoi and M.leptophylla[J]. Chinese Journal of Applied Ecology, 2009, 20(2): 271-276.
[19]	王玫, 尹承苗, 孙萌萌, 等. 黄腐酸微生物菌剂对连作平邑甜茶光合特性的影响[J]. 植物生理学报, 2019, 55(1): 99-106.
	WANG Mei, YIN Chengmiao, SUN Mengmeng, et al. Effect of fulvic acid on leaf photosynthesis fluorescent parameters of apple(Malus hupehensis) under replant condition[J]. Plant Physiology Journal, 2019, 55(1): 99-106.
[20]	陈芬, 余高, 陈容, 等. 减磷配施有机肥对辣椒生长及光合荧光特性的影响[J]. 西北农林科技大学学报(自然科学版), 2021, 49(7): 114-125.
	CHEN Fen, YU Gao, CHEN Rong, et al. Effects of reduced application of chemical phosphorus combined with organic fertilizer on growth and photosynthetic fluorescence characteristics of Capsicums annuum L[J]. Journal of Northwest A & F University (Natural Science Edition), 2021, 49(7): 114-125.
[21]	朱迎春, 安国林, 李卫华, 等. 不同生物有机肥对西瓜光合特性、产量和品质的影响[J]. 中国瓜菜, 2020, 33(10): 29-32.
	ZHU Yingchun, AN Guolin, LI Weihua, et al. Effects of different bio-organic fertilizers on photosynthetic character-istics, yield and quality of watermelon[J]. China Cucurbits and Vegetables, 2020, 33(10): 29-32.
[22]	王亚雄, 常少刚, 王锐. 不同有机肥对宁夏枸杞生长、产量及品质的影响[J]. 中国土壤与肥料, 2019, (5):91-95.
	WANG Yaxiong, CHANG Shaogang, WANG Rui, et al. Effects of different organic fertilizers on the growth, yield and quality of Ningxia wolfberry[J]. Soil and Fertilizer Sciences in China, 2019, (5):91-95.
[23]	王晓娟, 贾志宽, 梁连友, 等. 不同有机肥量对旱地玉米光合特性和产量的影响[J]. 应用生态学报, 2012, 23(2): 419-425.
	WANG Xiaojuan, JIA Zhikuan, LIANG Lianyou, et al. Effects of organic fertilizer application rate on leaf photosynthetic characteristics and grain yield of dryland maize[J]. Chinese Journal of Applied Ecology, 2012, 23(2): 419-425.

处理 Treatments	叶面积 Leaf area (cm²)	叶长 Leaf length (cm)	叶宽 Blade width (cm)	鲜重 Fresh weight (g)	干重 Dry weight (g)
CK	86.66±0.82^d	16.52±0.51^ab	7.84±0.35^b	1.20±0.03^c	0.24±0.04^d
T₁	98.38±4.32^bc	17.46±0.55^ab	8.02±0.21^b	1.45±0.10^bc	0.33±0.03^cd
T₂	92.63±4.01^cd	16.27±0.43^b	8.18±0.35^b	1.41±0.20^bc	0.44±0.06^bc
T₃	105.6±2.92^ab	16.18±0.65^b	9.42±0.54^a	1.93±0.29^ab	0.56±0.05^ab
T₄	99.28±3.76^bc	16.62±0.54^ab	8.48±0.24^ab	1.71±0.15^bc	0.39±0.05^cd
T₅	114.18±2.29^a	18.05±0.58^a	8.86±0.30^ab	2.29±0.16^a	0.64±0.08^a

处理 Treatments	叶面积 Leaf area (cm²)	叶长 Leaf length (cm)	叶宽 Blade width (cm)	鲜重 Fresh weight (g)	干重 Dry weight (g)
CK	86.66±0.82^d	16.52±0.51^ab	7.84±0.35^b	1.20±0.03^c	0.24±0.04^d
T₁	98.38±4.32^bc	17.46±0.55^ab	8.02±0.21^b	1.45±0.10^bc	0.33±0.03^cd
T₂	92.63±4.01^cd	16.27±0.43^b	8.18±0.35^b	1.41±0.20^bc	0.44±0.06^bc
T₃	105.6±2.92^ab	16.18±0.65^b	9.42±0.54^a	1.93±0.29^ab	0.56±0.05^ab
T₄	99.28±3.76^bc	16.62±0.54^ab	8.48±0.24^ab	1.71±0.15^bc	0.39±0.05^cd
T₅	114.18±2.29^a	18.05±0.58^a	8.86±0.30^ab	2.29±0.16^a	0.64±0.08^a

处理 Treatments	全氮 Total nitrogen (g/kg)	全磷 Phosphorus (g/kg)	全钾 Total potassium (g/kg)
CK	12.53±0.15^c	1.57±0.12^c	10.44±0.06^d
T₁	16.47±0.23^b	1.83±0.03^b	12.23±0.23^c
T₂	17.27±0.27^b	1.73±0.03^b	12.59±0.24^c
T₃	17.50±0.23^b	2.12±0.03^ab	15.21±0.03^ab
T₄	19.77±2.14^a	2.11±0.06^ab	14.63±0.10^b
T₅	20.76±0.15^a	2.53±0.03^a	16.44±0.06^a

处理 Treatments	全氮 Total nitrogen (g/kg)	全磷 Phosphorus (g/kg)	全钾 Total potassium (g/kg)
CK	12.53±0.15^c	1.57±0.12^c	10.44±0.06^d
T₁	16.47±0.23^b	1.83±0.03^b	12.23±0.23^c
T₂	17.27±0.27^b	1.73±0.03^b	12.59±0.24^c
T₃	17.50±0.23^b	2.12±0.03^ab	15.21±0.03^ab
T₄	19.77±2.14^a	2.11±0.06^ab	14.63±0.10^b
T₅	20.76±0.15^a	2.53±0.03^a	16.44±0.06^a