Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (3): 584-590.DOI: 10.6048/j.issn.1001-4330.2024.03.007

• Crop Genetics and Breeding·Germplasm Resources·Molecular Genetics·Physiology and Biochemistry • Previous Articles     Next Articles

Identification of disease resistance of Xinjiang wheat based on KASP technology

GAO Xin1(), SHI Jia1, WANG Chunsheng1, WANG Zhong1, LI Jianfeng1, LI Ailing2, FAN Zheru1(), ZHANG Yueqiang1()   

  1. 1. Key Laboratory of Desert-Oasis Crop Physiology,Ecology and Tillage,MOAR/Research Institute of Nuclear and Biological Technology,Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Crop Biotechnology/Xinjiang Crop Chemical Regulation Engineering Technology Researching Center,Urumqi 830091, China
    2. School of Science and Technology,Xinjiang Agricultural University,Urumqi 830052,China
  • Received:2023-07-20 Online:2024-03-20 Published:2024-04-19
  • Correspondence author: FAN Zheru(1964-),male,from Xinjiang,researcher,research direction:wheat genetics and breeding,(E-mail)fzr640814@163.com;ZHANG Yueqiang(1976-),male,from Xinjiang,researcher,research direction:wheat genetics and breeding,(E-mail) zhangyqyhm@163.com
  • Supported by:
    Xinjiang Production and Construction Corps Agricultrual Core Tackling Project(NYHXG-2023AA201);Key Laboratory of Crop Biotechnology in Xinjiang(XNYPT-2021-004);XinJiang Agriculture Research System(XJARS-01)

基于KASP技术鉴定新疆小麦抗病性相关基因

高新1(), 时佳1, 王春生1, 王重1, 李剑峰1, 李艾玲2, 樊哲儒1(), 张跃强1()   

  1. 1.新疆农业科学院核技术生物技术研究所/农业部荒漠绿洲作物生理生态与耕作重点实验室/新疆农作物生物技术重点实验室/新疆作物化学调控工程技术研究中心,乌鲁木齐 830091
    2.新疆农业大学科学技术学院,乌鲁木齐 830052
  • 通讯作者: 樊哲儒(1964- ),男,新疆人,研究员,研究方向为小麦育种与栽培,(E-mail)fzr640814@qq.com;张跃强(1976- ),男,新疆人,研究员,硕士生导师,研究方向为小麦育种与栽培,(E-mail)zhangyqyhm@163.com
  • 作者简介:高新(1988- ),女,新疆人,助理研究员,研究方向为小麦育种与栽培,(E-mail)gaoxin0564@163.com
  • 基金资助:
    新疆生产建设兵团农业核心攻关项目(NYHXG-2023AA201);新疆农作物生物技术重点实验室开放课题(XNYPT-2021-004);新疆现代农业小麦产业技术体系(XJARS-01)

Abstract:

【Objective】 This study aims to identify the genes related to disease resistance of Xinjiang wheat varieties(lines) and provide materials for genetic improvement of wheat breeding.【Methods】 In the present study,KASP technology was used to detect the genes related to disease resistance in 183 Xinjiang wheat varieties(lines).【Results】 The results showed that 99 varieties had the leaf rust resistance gene Lr46 with a frequency of 54.10%,64 varieties had the leaf rust resistance gene Lr14a with a frequency of 34.97%,3 varieties had the leaf rust resistance gene Lr68,no variety had the powdery mildew resistance gene Pm21 and yellow rust resistance gene Yr15.The distribution frequency of resistance genes in Xinjiang wheat varieties(lines) was low with a frequency of about 26.8%.【Conclusion】 The 146 selected materials could be used as resistance materials for wheat breeding of long term rust resistance in Xinjiang.

Key words: wheat; disease resistance; KASP; mark detection

摘要:

【目的】 鉴定新疆小麦审定品种(系)抗病性相关基因,为小麦育种遗传改良提供材料。【方法】 利用KASP技术对183份新疆小麦品种(系)的抗病性相关基因进行检测。【结果】 99份材料携带抗叶锈病基因Lr46,频率为54.10%,64份材料携带抗叶锈病基因 Lr14a,频率为34.97%,3份材料携带抗叶锈病基因Lr68,0份材料携带抗白粉病基因 Pm21 。新疆小麦品种(系)中抗病基因的分布频率较低,约为 26.8% 。【结论】 筛选出的 146 份材料可以作为新疆小麦持久抗锈病育种的抗源材料。

关键词: 小麦, 抗病性, KASP, 标记检测

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