Identification and genetic diversity of wild Macrolepiota in Xinjiang

doi:10.6048/j.issn.1001-4330.2023.10.020

Abstract

Abstract:

【Objective】 To identify and analyze their genetic diversity of wild strains of Macrolepiota in Xinjiang. Which can be used as a basis for the selection of parental materials for the next breeding research.【Methods】 The ITS sequence was used to identify the species and the ISSR markers technology applied to carry out the genetic diversity analysis on 17 wild Macrolepiota strains.【Results】 The 17 tested wild strains were the Macrolepiota excoriate, the screened-out 10 ISSR primers amplified a total of 116 clear DNA bands, including 75 polymorphic bands with a polymorphism ratio of 64.66%.The genetic similarity coefficient of each strain ranged from 0.71 to 0.99, and the 17 tested strains were divided into 8 groups at the genetic similarity coefficient of 0.85.【Conclusion】 Macrolepiota excoriataalso is distributed in Xinjiang.and it has the occurrence of genetic differentiation with relatively rich genetic diversity.

Key words: Macrolepiota excoriata; ITS; ISSR; genetic diversity

摘要：

【目的】 从分子水平研究鉴定新疆野生大环柄菇属菌株,分析其遗传多样性,为育种亲本材料的选择提供依据。【方法】 采用ITS序列测定技术对供试菌株进行物种鉴定,并利用ISSR标记技术对17个野生大环柄菇菌株进行遗传多样性分析。【结果】 供试的17个菌株均为裂皮大环柄菇Macrolepiota excoriata。筛选出的10条ISSR引物共扩增出了116条重复性较好,清晰的DNA条带,多态性条带总数为75条,多态比率约为64.66%。各供试菌株之间遗传相似性系数范围约在 0.71~0.99,当遗传相似性系数为0.85时,17个供试菌株可分为8个类群。【结论】 裂皮大环柄菇在新疆也有分布,且其野生菌株具有比较丰富的遗传多样性。

关键词: 大环柄菇, ITS, ISSR, 遗传多样性

CLC Number:

S646

LUO Ying, Nurziya Yalimaimaiti, JIA Wenjie, LIU Jieying, JIA Peisong. Identification and genetic diversity of wild Macrolepiota in Xinjiang[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2501-2508.

罗影, 努尔孜亚·亚力买买提, 贾文捷, 刘洁莹, 贾培松. 新疆野生大环柄菇的分子鉴定与遗传多样性分析[J]. 新疆农业科学, 2023, 60(10): 2501-2508.

Figures/Tables 8

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序号 No.	引物编号 Primer No.	引物序列 Primer sequence (5'-3')	退火温度 Annea- ling temper- ature (℃)
1	P2	GTGACACACACACAC	56
2	P4	GGATGCAACACACACACAC	55
3	P5	CGTGTGTGTGTGTGT	48
4	P20	ACACACACACACACACCTG	52
5	P21	AGCAGCAGCAGCAGCAGCG	56
6	P22	AAGAAGAAGAAGAAGAAGC	50
7	P25	GAGAGAGAGAGAGAGACC	56
8	P26	CACCACACACACACACA	50
9	P807	AGAGAGAGAGAGAGAGT	55
10	P864	ATGATGATGATGATGATG	50

序号 No.	引物编号 Primer No.	引物序列 Primer sequence (5'-3')	退火温度 Annea- ling temper- ature (℃)
1	P2	GTGACACACACACAC	56
2	P4	GGATGCAACACACACACAC	55
3	P5	CGTGTGTGTGTGTGT	48
4	P20	ACACACACACACACACCTG	52
5	P21	AGCAGCAGCAGCAGCAGCG	56
6	P22	AAGAAGAAGAAGAAGAAGC	50
7	P25	GAGAGAGAGAGAGAGACC	56
8	P26	CACCACACACACACACA	50
9	P807	AGAGAGAGAGAGAGAGT	55
10	P864	ATGATGATGATGATGATG	50

序号 No.	菌株 Strain	采集地	菌落形态 Colony morphology	菌丝长势 The growth potentia	菌丝生长速度 Growth vigor(mm/d)
1	HBG001	哈巴河县	菌丝浓密,边缘整齐	+++	1.49±0.08^ef
2	HBG002	哈巴河县	菌丝较浓密,边缘整齐	+++	1.99±0.09^ab
3	HBG005	哈巴河县	菌丝浓密,边缘不整齐	+	1.44±0.03^f
4	HBG006	哈巴河县	菌丝浓密,边缘不整齐	++	1.94±0.18^bc
5	HBG008	特克斯县	菌丝浓密,边缘不整齐	++	1.69±0.33^de
6	HBG009	特克斯县	菌丝较浓密,边缘不整齐	++	1.37±0.19^fgh
7	HBG010	哈巴河县	菌丝浓密,边缘不整齐	++	1.42±0.08^fg
8	HBG011	哈巴河县	菌丝浓密,边缘不整齐	++	1.47±0.15^ef
9	HBG012	哈巴河县	菌丝浓密,边缘不整齐	++	1.13±0.10
10	HBG013	哈巴河县	菌丝浓密,边缘不整齐	++	1.20±0.05^ghi
11	HBG014	哈巴河县	菌丝浓密,边缘不整齐	++	1.09±0.12
12	HBG015	哈巴河县	菌丝浓密,边缘不整齐	++	1.14±0.06^hi
13	HBG016	哈巴河县	菌丝浓密,边缘整齐	++++	2.17±0.09^a
14	HBG017	哈巴河县	菌丝浓密,边缘不整齐	++	1.21±0.10^ghi
15	HBG018	哈巴河县	菌丝浓密,边缘不整齐	++	1.19±0.06^ghi
16	HBG019	哈巴河县	菌丝浓密,边缘不整齐	+++	1.74±0.22^cd
17	HBG020	哈巴河县	菌丝浓密,边缘不整齐	++	1.04±0.18

序号 No.	菌株 Strain	采集地	菌落形态 Colony morphology	菌丝长势 The growth potentia	菌丝生长速度 Growth vigor(mm/d)
1	HBG001	哈巴河县	菌丝浓密,边缘整齐	+++	1.49±0.08^ef
2	HBG002	哈巴河县	菌丝较浓密,边缘整齐	+++	1.99±0.09^ab
3	HBG005	哈巴河县	菌丝浓密,边缘不整齐	+	1.44±0.03^f
4	HBG006	哈巴河县	菌丝浓密,边缘不整齐	++	1.94±0.18^bc
5	HBG008	特克斯县	菌丝浓密,边缘不整齐	++	1.69±0.33^de
6	HBG009	特克斯县	菌丝较浓密,边缘不整齐	++	1.37±0.19^fgh
7	HBG010	哈巴河县	菌丝浓密,边缘不整齐	++	1.42±0.08^fg
8	HBG011	哈巴河县	菌丝浓密,边缘不整齐	++	1.47±0.15^ef
9	HBG012	哈巴河县	菌丝浓密,边缘不整齐	++	1.13±0.10
10	HBG013	哈巴河县	菌丝浓密,边缘不整齐	++	1.20±0.05^ghi
11	HBG014	哈巴河县	菌丝浓密,边缘不整齐	++	1.09±0.12
12	HBG015	哈巴河县	菌丝浓密,边缘不整齐	++	1.14±0.06^hi
13	HBG016	哈巴河县	菌丝浓密,边缘整齐	++++	2.17±0.09^a
14	HBG017	哈巴河县	菌丝浓密,边缘不整齐	++	1.21±0.10^ghi
15	HBG018	哈巴河县	菌丝浓密,边缘不整齐	++	1.19±0.06^ghi
16	HBG019	哈巴河县	菌丝浓密,边缘不整齐	+++	1.74±0.22^cd
17	HBG020	哈巴河县	菌丝浓密,边缘不整齐	++	1.04±0.18

序号 No.	引物编号 Primer No.	扩增条带数 No.of bands(n)	多态性条带数 No.of polymorphic bands (k)	多态位点比率 Fraction of polymorphic ( p /100% )
1	P2	7	3	42.86
2	P4	14	11	78.57
3	P5	13	9	69.23
4	P20	11	8	72.73
5	P21	9	3	37.50
6	P22	9	5	62.50
7	P25	11	3	27.27
8	P26	15	13	86.67
9	P807	15	13	86.67
10	P864	12	7	58.33
总计Total		116	75	64.66
平均Average		11.6	7.5	64.66