Relationship between electrical conductivity of seed soaking solution and seed viability in wheat spike parts

doi:10.6048/j.issn.1001-4330.2023.10.008

Abstract

Abstract:

【Objective】 To investigate the feasibility of conductivity prediction of seed vigor in wheat spike parts and to provide a theoretical basis for rapid prediction of seed vigor.【Methods】 The spike parts were all divided into six samples at 15 d (A₁₅), 20 d (A₂₀), 25 d (A₂₅), 30 d (A₃₀), 35 d (A₃₅), 40 d (A₄₀) and 45 d (A₄₅) after flowering under seven different harvesting time conditions, namely, B₁, B₂, B₃, B₄, B₅ and B₆, respectively, using Xinjiang main cultivar Xinchun 44 wheat variety as the material. A wide range of seed vigor levels was formed to investigate the relationship between electrical conductivity and seed vigor in the spike parts. 【Results】 The results showed that seed conductivity and seedling height, seedling fresh weight, seedling dry weight, germination potential, germination percentage, germination index, vigor index and simplified vigor index reached highly significant levels. The coefficients of determination (R²) of the regression models were 0.656 8,0.811 6, 0.864, 0.802 7 and 0.803 6.【Conclusion】 Conductivity determination was simple and easy, and there were good linear relationships between conductivity and seedling height, seedling fresh weight, seedling dry weight,vigor index and simplified vigor index, respectively, so the method of conductivity determination was able to predict seed viability of the spike parts.

Key words: wheat; spike parts; electrical conductivity; seed viability

摘要：

【目的】 研究电导率预测小麦穗部位种子活力的可行性,为快速预测种子活力提供理论依据。【方法】 以新疆小麦主栽品种新春44号为材料,在花后15 d(A₁₅)、20 d(A₂₀)、25 d(A₂₅)、30 d(A₃₀)、35 d(A₃₅)、40 d(A₄₀)和45 d(A₄₅) 7个不同收获时间条件下,分别将穗部位均分为B₁、B₂、B₃、B₄、B₅和B₆ 6个样本,形成宽广的种子活力水平范围,研究穗部位电导率与种子活力之间的关系。【结果】 种子电导率与苗高、苗鲜重、苗干重、发芽势、发芽率、发芽指数、活力指数和简化活力指数均达到极显著水平。回归模型的决定系数(R²)分别为0.656 8、0.811 6、0.864、0.802 7和0.803 6。【结论】 电导率测定简单易行,电导率与苗高、苗鲜重、苗干重、活力指数和简化活力指数间有良好的线性关系,用测定电导率的方法能够预测穗部位种子的活力。

关键词: 小麦, 穗部位, 电导率, 种子活力

CLC Number:

S512

ZHANG Lulu, ZHANG Jinshan, JIA Yonghong, LI Peng, Gulinigaer Turghun, WANG Runqi, DONG Yanxue, SHI Shubing. Relationship between electrical conductivity of seed soaking solution and seed viability in wheat spike parts[J]. Xinjiang Agricultural Sciences, 2023, 60(10): 2404-2411.

张路路, 张金汕, 贾永红, 李鹏, 古力尼尕尔·吐尔洪, 王润琪, 董艳雪, 石书兵. 小麦穗部位种子浸泡液电导率与种子活力的关系[J]. 新疆农业科学, 2023, 60(10): 2404-2411.

Figures/Tables 4

References 23

[1]	孙群, 王建华, 孙宝启. 种子活力的生理和遗传机理研究进展[J]. 中国农业科学, 2007,(1):48-53.
	SUN Qun, WANG Jianhua, SUN Baoqi. Advances on Seed Vigor Physiological and Genetic Mechanisms[J]. Scientia Agricultura Sinica, 2007,(1):48-53.
[2]	Al-Amery M, Geneve R L, Sanches M F, et al. Near-infrared spectroscopy used to predict soybean seed germination and vigour[J]. Seed Science Research, 2018, 28:245-252. DOI URL
[3]	Ellis R H. Seed and seedling vigour in relati on to crop growth and yield[J]. Plant Growth Regulation, 1992, 11(3):249-255. DOI URL
[4]	马守才, 张改生, 王军卫, 等. 小麦种子活力性状的遗传变异和相关研究(英文)[J]. 西北植物学报, 2004,(9):1674-1679.
	MA Shoucai, ZHANG Gaisheng, WANG Junwei, et al. Genetic difference and interrelationship among seed vigor traits in wheat[J]. Acta Botanica Boreali-Occidentalia Sinica, 2004,(9):1674-1679.
[5]	张艳芳, 吴凯铭, 刘光耀, 等. 不同收获期对小麦种子活力及物理性状的影响[J]. 种子, 2021, 40(8):1-7,14.
	ZHANG Yanfang, WU Kaiming, LIU Guanyao, et al. Effects of different time on vigor and physical characteristics of wheat seeds[J]. Seed, 2021, 40(8):1-7,14.
[6]	Fick G L, Hibbard R P. A method for determining seed viability by el ectri cal conductivity measurements[J]. Michigan Academy of Sciences, Arts and Letters, 1925, 5: 95-103.
[7]	Matthews S, Powell A A. Electrical conductivity vigour test: Physiological basis and use[J]. Seed Testing International, 2006, 131:32-35.
[8]	朱银, 颜伟, 杨欣, 等. 电导法测定小麦种子活力[J]. 江苏农业科学, 2014, 42(9):78-80.
	ZHU Yin, YAN Wei, YANG Xin, et al. Determination of wheatseed vigor by conductance method[J]. Jiangsu Agricultural Sciences, 2014, 42(9):78-80.
[9]	郑雅潞, 薛小雁, 仇永康, 等. 小麦种子活力测定方法的比较[J]. 江苏农业科学, 2017, 45(15):61-64.
	ZHENG Yalu, XUE Xiaoyan, QIU Yongkang, et al. Comparison of wheat seed vigor determination methods[J]. Jiangsu Agricultural Sciences, 2017, 45(15):61-64.
[10]	杨剑平, 唐玉林, 王文平. 小麦种子衰老的生理生化分析[J]. 种子, 1995,(2):13-14.
	YANG Jianping, TANG Yulin, WANG Wenping. Analysis of physiology and biochemistry for senescence of wheat seed[J]. Seed, 1995,(2):13-14.
[11]	李富花, 李富利, 李富荣, 等. 燕麦种子活力测定方法[J]. 草业科学, 2013, 30(5):767-771.
	LI Fuhua, LI Fuli, LI Furong, et al. Study on testing methods of oat seed vigour[J]. Pratacultural Science, 2013, 30(5):767-771.
[12]	冯魁, 贾永红, 李琼, 等. 春小麦新春31号种子活力与穗位和籽粒成熟度的关系[J]. 麦类作物学报, 2017, 37(6):808-814.
	FENG Kui, JIA Yonghong, LI Qiong, et al. Relationship between seed vigor of the sprin wheat Xinchun 31 and ear axis position and seed maturity[J]. Journal of Triticeae Crops, 2017, 37(6):808-814.
[13]	刘旭欢, 加得拉·努尔兰, 贾永红, 等. 籽粒成熟度与穗部位置对春小麦种子活力的影响[J]. 西北农业学报, 2014, 23(10):71-75.
	LIU Xuhuan, Jiadela Nuerlan, JIA Yonghong, et al. Effects of maturity and head position on seed vigor of spring wheat[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2014, 23(10):71-75.
[14]	周爱清, 罗顺. 种子活力[M]. 北京: 中国农业出版社, 1990.
	ZHOU Aiqing, LUO Shun. Seed Vigor[M]. Beijing: China Agriculture Press, 1990.
[15]	Mavi K, Mavi F, Demir I, et al. Electrical conductivity of seed soak water predicts seedling emergence and seed storage potential in commercial seed lots of radish[J]. Seed Science and Technology, 2014, 42(1):76-86. DOI URL
[16]	余波, 杜尚广, 罗丽萍. 种子活力测定方法[J]. 中国科学:生命科学, 2015, 45:709-713.
	YU Bo, DU Shangguang, LUO Liping. Progress in determinations of seed vigor[J]. Scientia Sinica:Vitae, 2015, 45:709-713.
[17]	Wang Y, Yu L, Nan Z, et al. Vigor tests used to rank seed lot quality and predict field emergence in four forage species[J]. Crop Science, 2004, 44(2):535-541. DOI URL
[18]	郑成超, 邹琦, 程炳嵩. 大豆种子吸胀时内含物外渗及其与种子活力的相关性研究[J]. 大豆科学, 1991, 10(3):226-230.
	ZHENG Chenchao, ZOU Qi, CHENG Bingsong. Study on the exudation of inclusions during swelling of soybean seeds and its correlation with seed vigor[J]. Soybean Science, 1991, 10(3):226-230.
[19]	Larry O C, Copeland M, Miller B. Principles of Seed Science and Technology (3rd. Ed.)[M]. Springer, Boston, MA, 1999.
[20]	张红生. 种子学[M]. 北京: 科学出版社, 2015.
	ZHANG Hongsheng. Seed Scoience[M]. Beijing: Science Press, 2015.
[21]	曲宗普尺, 毛光锋, 吴敏, 等. 水稻种子浸泡液电导率与种子活力的关系[J/OL]. 中国农业科技导报:1-7[2022-05-30].
	Quzongpuchi, WAO Guanfeng, WU Min, et al. Relationship Between Electrical Conductivity of Seed Soaking Solution and Seed Vigor in Rice(Oryza sativa L.)[J/OL]. Journal of Agricultural Science and Technology: 1-7[2022-05-30].
[22]	张文明, 倪安丽, 王昌初, 等. 杂交水稻及其双亲种子活力的研究[J]. 安徽农业大学学报, 1997,(1):42-46.
	ZHANG Wenming, NI Anli, Wang Changchu, et al. Study on seed viability of hybrid rice and its two parents[J]. Journal of Anhui Agricultural University, 1997,(1):42-46.
[23]	卢新雄, 辛霞, 尹广鹍, 等. 中国作物种质资源安全保存理论与实践[J]. 植物遗传资源学报, 2019, 20(1):1-10.
	LU Xinxiong, XIN Xia, YIN Guangkun, et al. Theory and Practice of the Safe Conservation of Crop Germplasm Resources in China[J]. Journal of Plant Genetic Resources, 2019, 20(1):1-10.

性状 Trait	变幅 Range	平均值 Mean	标准差 Standard deviation
苗高Seed height(mm)	37.08~86.08	67.90	11.19
苗鲜重Seedling fresh weight(mg)	17.02~67.10	49.23	13.34
苗干重Seedling dry weight(mg)	1.49~7.09	5.06	1.52
发芽势Germination energy(%)	0.59~0.95	0.81	0.08
发芽率Germination rate(%)	0.61~0.96	0.84	0.08
发芽指数Germination index	24.32~38.80	32.95	3.41
活力指数Vigor index	459.25~2498.35	1645.32	525.25
简化种子活力指数Simplified vigor inde	12.71~62.31	41.70	13.14
电导率Electrical conductivity(μS/(cm·g))	11.26~80.56	29.24	21.46

性状 Trait	变幅 Range	平均值 Mean	标准差 Standard deviation
苗高Seed height(mm)	37.08~86.08	67.90	11.19
苗鲜重Seedling fresh weight(mg)	17.02~67.10	49.23	13.34
苗干重Seedling dry weight(mg)	1.49~7.09	5.06	1.52
发芽势Germination energy(%)	0.59~0.95	0.81	0.08
发芽率Germination rate(%)	0.61~0.96	0.84	0.08
发芽指数Germination index	24.32~38.80	32.95	3.41
活力指数Vigor index	459.25~2498.35	1645.32	525.25
简化种子活力指数Simplified vigor inde	12.71~62.31	41.70	13.14
电导率Electrical conductivity(μS/(cm·g))	11.26~80.56	29.24	21.46

性状 Trait	类型 Types	变幅 Range	平均值 Mean	标准差 Standard deviation	F值
	第1类	63.98~86.08	73.12	5.46
苗高Seed height(mm)	第2类	37.08~52.39	44.96	6.02	39.47^**
	第3类	42.74~74.27	65.29	9.28
	第1类	42.22~67.10	56.56	5.22
苗鲜重Seedling fresh weight(mg)	第2类	17.02~28.64	22.03	4.29	61.476^**
	第3类	18.67~55.05	43.03	10.40
	第1类	4.30~7.09	5.96	0.61
苗干重Seedling dry weight(mg)	第2类	1.49~2.44	2.05	0.35	80.382^**
	第3类	1.85~5.28	4.13	0.97
	第1类	0.71~0.95	0.84	0.06
发芽势Germination energy(%)	第2类	0.74~0.81	0.78	0.03	6.902^**
	第3类	0.59~0.88	0.75	0.09
	第1类	0.72~0.96	0.87	0.06
发芽率Germination rate(%)	第2类	0.75~0.84	0.80	0.04	10.03^**
	第3类	0.61~0.88	0.77	0.09
	第1类	28.72~38.8	34.35	2.54
发芽指数Germination index	第2类	27.3~31.81	30.10	1.86	8.817^**
	第3类	24.32~36.57	30.61	4.03
	第1类	1208.68~2498.35	1946.05	261.38
活力指数vigor index	第2类	459.25~890.47	667.28	154.15	52.972^**
	第3类	505.64~1652.51	1322.37	360.9
简化种子活力指数 Simplified vigor inde	第1类	30.36~62.31	49.36	6.66	54.298^**

性状 Trait	类型 Types	变幅 Range	平均值 Mean	标准差 Standard deviation	F值
	第1类	63.98~86.08	73.12	5.46
苗高Seed height(mm)	第2类	37.08~52.39	44.96	6.02	39.47^**
	第3类	42.74~74.27	65.29	9.28
	第1类	42.22~67.10	56.56	5.22
苗鲜重Seedling fresh weight(mg)	第2类	17.02~28.64	22.03	4.29	61.476^**
	第3类	18.67~55.05	43.03	10.40
	第1类	4.30~7.09	5.96	0.61
苗干重Seedling dry weight(mg)	第2类	1.49~2.44	2.05	0.35	80.382^**
	第3类	1.85~5.28	4.13	0.97
	第1类	0.71~0.95	0.84	0.06
发芽势Germination energy(%)	第2类	0.74~0.81	0.78	0.03	6.902^**
	第3类	0.59~0.88	0.75	0.09
	第1类	0.72~0.96	0.87	0.06
发芽率Germination rate(%)	第2类	0.75~0.84	0.80	0.04	10.03^**
	第3类	0.61~0.88	0.77	0.09
	第1类	28.72~38.8	34.35	2.54
发芽指数Germination index	第2类	27.3~31.81	30.10	1.86	8.817^**
	第3类	24.32~36.57	30.61	4.03
	第1类	1208.68~2498.35	1946.05	261.38
活力指数vigor index	第2类	459.25~890.47	667.28	154.15	52.972^**
	第3类	505.64~1652.51	1322.37	360.9
简化种子活力指数 Simplified vigor inde	第1类	30.36~62.31	49.36	6.66	54.298^**