Effects of High Temperature Stress on Photosynthetic and Fluorescence Characteristics of Centennial Seedless and Wuhe Cuibao Grape Leaves

doi:10.6048/j.issn.1001-4330.2022.08.012

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (8): 1919-1928.DOI: 10.6048/j.issn.1001-4330.2022.08.012

• Horticultural Special Local Products·Germplasm Resources·Storage and Preservation Processing·Soil Fertilizer • Previous Articles Next Articles

Effects of High Temperature Stress on Photosynthetic and Fluorescence Characteristics of Centennial Seedless and Wuhe Cuibao Grape Leaves

NIU Qian¹^,²(), ZHANG Wen², HAN Shouan², WANG Min², WANG Wenyong³, FENG Fuji³, WANG Huan³, GONG Jiaqi³, LI Jiaying³, TIAN Jia¹(), XIE Hui²()

1. College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China
2. Institute of Horticultural Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
3. Department of Biological Science, College of Science and Technology Xinjiang Agricultural University, Urumqi 830052, China

Received:2021-11-10 Online:2022-08-20 Published:2022-10-01
Correspondence author: TIAN Jia, XIE Hui
Supported by:
National Natural Science Foundation of China(31960577);Science and Technology Innovation Key Project of Xinjiang Academy of Agricultural Sciences(xjkcpy-2020001);China Agriculture Research System(CARS-29-ZP-08)

高温胁迫对无核白鸡心和无核翠宝葡萄叶片光合及荧光特性的影响

牛倩¹^,²(), 张雯², 韩守安², 王敏², 王文勇³, 冯福基³, 王环³, 弓佳琪³, 李佳颖³, 田嘉¹(), 谢辉²()

1.新疆农业大学林学与园艺学院,乌鲁木齐 830052
2.新疆农业科学院园艺作物研究所,乌鲁木齐 830091
3.新疆农业大学科技学院生物科学系,乌鲁木齐 830052

通讯作者: 田嘉,谢辉
作者简介:牛倩（1996-）,女,硕士研究生,研究方向为果树栽培与生理,（E-mail） 1078824364@qq.com
基金资助:
国家自然科学基金(31960577);新疆农业科学院科技创新重点培育专项(xjkcpy-2020001);现代农业产业技术体系专项资金(CARS-29-ZP-08)

Abstract

Abstract:

【Objective】 To investigate the effect of high temperature stress on its photosynthesis and photosynthetic characteristics through the changes of photosynthetic and fluorescence characteristics of Centennial Seedless and Wuhe Cuibao grapes under different light and temperature conditions. 【Methods】 In the experiment, the Centennial Seedless and Wuhe Cuibao grapes were used as materials, and the photosynthetic indexes were measured by instruments such as LI-6400, FMS-2. The effect of high temperature stress on photosynthesis of Centennial Seedless and Wuhe Cuibao grapes were comprehensively analyzed.【Results】 During the grape growth and development period（April to September） in 2020,the highest temperature in the high temperature environment was 43 ℃,the number of days when the highest daily temperature exceeded 40 ℃was 16 days;the highest temperature in the suitable temperature wasonly 38 ℃.And the highest PAR light radiation intensity values in high and suitable temperature were 1,887 and 1,730 µmol/（m²·s）, respectively. The diurnal variation of net photosynthetic rate of two cultivars both showed bimodal curve. At 10：00 the high temperature environmentPn reached up to 18.9 µmol/（m²·s）, which was higher than that 13.0 µmol/（m²·s） in the suitable temperature. The light compensation point of ‘Centennial Seedless’ was higher in the suitable temperature environment, while Wuhe Cuibao was in the high temperature environment; the apparent quantum efficiency and dark respiration rate of the two varieties were higher in the high temperature environment. The qP and NPQ values of Wuhe Cuibao in high temperature environment were higher than suitable environment; ΦPSⅡ value and electron transfer rate of Centennial Seedless in high temperature environment significantly were greater than the suitable environment.【Conclusion】 In the high temperature environment, the net photosynthetic rate of Centennial Seedless is the highest, and its photosynthetic capacity is better.The variety can grow well in the high temperature environment because it has adapted to high temperature areas.

Key words: high temperature stress; grape; light-response curve; diurnal variation of photosynthesis; luorescence parameters

摘要：

【目的】分析不同光温条件下无核白鸡心和无核翠宝葡萄光合特性与荧光特性的变化,研究高温胁迫对无核白鸡心和无核翠宝葡萄光合作用的影响,以及高温对无核白鸡心和无核翠宝葡萄光合特性的影响。【方法】以无核白鸡心和无核翠宝葡萄为材料,采用LI-6400,FMS-2等仪器测定光合指标,分析高温胁迫对无核白鸡心和无核翠宝葡萄光合作用的影响。【结果】2020年葡萄生长发育期（4~9月）,高温环境的日最高温度43℃,日最高温超过40 ℃的天数为16 d;适温环境的日最高温仅38 ℃。高温环境与适温环境最高PAR光辐射强度值分别为1 887 和1 730 µmol/（m²·s）;2个品种的净光合速率日变化均呈双峰曲线,在10：00时高温环境Pn高达18.9 µmol/（m²·s）,高于适温环境13.0 µmol/（m²·s）。无核白鸡心在适温环境下光补偿点较高,无核翠宝在高温环境光补偿点较高;2个品种表观量子效率和暗呼吸速率在高温环境较高。无核翠宝qP和NPQ值在高温环境中高于适宜环境;无核白鸡心在高温环境中ΦPSⅡ值和电子传递速率值高于适宜环境。【结论】无核白鸡心在高温环境净光合速率最高,光合能力表现较好,长期在高温环境下生长已经适应高温。

关键词: 高温胁迫, 葡萄, 光响应曲线, 光合日变化, 荧光参数

CLC Number:

S663.1

NIU Qian, ZHANG Wen, HAN Shouan, WANG Min, WANG Wenyong, FENG Fuji, WANG Huan, GONG Jiaqi, LI Jiaying, TIAN Jia, XIE Hui. Effects of High Temperature Stress on Photosynthetic and Fluorescence Characteristics of Centennial Seedless and Wuhe Cuibao Grape Leaves[J]. Xinjiang Agricultural Sciences, 2022, 59(8): 1919-1928.

牛倩, 张雯, 韩守安, 王敏, 王文勇, 冯福基, 王环, 弓佳琪, 李佳颖, 田嘉, 谢辉. 高温胁迫对无核白鸡心和无核翠宝葡萄叶片光合及荧光特性的影响[J]. 新疆农业科学, 2022, 59(8): 1919-1928.

Figures/Tables 7

Fig. 1 Diurnal variation of temperature, humidity and photosynthetically active radiation in different cultivation regions Note： GW： High temperature environment; SW： Suitable temperature environment; A： Daily maximum temperature; B： Daily minimum temperature; C： Maximum daily humidity; D： Daily minimum humidity; E： Daily mean temperature： F：Daily mean humidity; G：Photosynthetically active radiation.

Fig. 2 Yevin and diurnal variation of net photosynthetic rate Note： GW-1： High temperature environment-Centennial Seedless; SS-2：High temperature environment-Wuhe Cuibao; SW-1：Suitable temperature environment-Centennial Seedless; SW-2：Suitable temperature environment-Wuhe Cuibao.

Fig. 3 Response curve of photosynthetic parameters of leaves

Fig. 4 comparisonoffourmodelsforleaflightresponsecurvesimulation

Fig. 5 Linear and nonlinear fitting of net photosynthetic rate response to light intensity by rectangular hyperbolic correction model

Table 1 The Parameters of photosynthetic rate response to light intensity

处理 Treatment	最大净光合速率 pmax μmol/ （m²·s）	光饱和点LSP μmol/ （m²·s）	光补偿点LCP μmol/ （m²·s）	量子效率AQY μmol/ （m²·s）	暗呼吸Rd μmol/（m²·s）
GW-1	19.072	1196.671	30.431	0.1482	3.926
SW-1	08.751	1099.521	36.610	0.0495	1.625
GW-2	12.464	1142.663	38.733	0.1151	3.674
SW-2	13.537	1196.910	24.206	0.0737	1.651

Table 2 Chlorophyllfluorescenceparameters

处理 Treatment	PSⅡ实际光化学效率 ΦPSⅡ	光化学猝灭系数 qP	非光化学猝灭系数 NPQ	电子传递速率 ETR	PSⅡ最大光化学效率Fv/Fm
GW-1	0.824±0.031^a	1.069±0.081^b	0.972±0.539^ab	3.807±0.144^a	0.896±0.011^a
SW-1	0.613±0.087^b	1.186±0.064^b	2.193±0.41^a	2.261±0.273^c	0.851±0.034^a
GW-2	0.703±0.044a^b	2.847±0.738^a	2.285±0.389^a	3.544±0.221^ab	0.779±0.04^a
SW-2	0.820±0.048^a	1.048±0.048^b	0.522±0.082^b	2.929±0.171^bc	0.859±0.055^a

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Effects of High Temperature Stress on Photosynthetic and Fluorescence Characteristics of Centennial Seedless and Wuhe Cuibao Grape Leaves

高温胁迫对无核白鸡心和无核翠宝葡萄叶片光合及荧光特性的影响

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