Xinjiang Agricultural Sciences ›› 2024, Vol. 61 ›› Issue (1): 109-117.DOI: 10.6048/j.issn.1001-4330.2024.01.012
• Horticultural Special Local Products·Agricultural Product Processing Engineering • Previous Articles Next Articles
YANG Bo(), WANG Hao, CHEN Yongwei, HA Rong, JIN Wei, XU Can, ZHANG Min, YANG Guili, YANG Hongbo, MA Wenli(
)
Received:
2023-04-20
Online:
2024-01-20
Published:
2024-02-21
Correspondence author:
MA Wenli(1975-),male,Ningxia,researcher,research direction:crop cultivation,(E-mail) Supported by:
杨波(), 王昊, 陈永伟, 哈蓉, 靳韦, 徐灿, 张敏, 杨桂丽, 杨宏波, 马文礼(
)
通讯作者:
马文礼(1975-),男,宁夏平罗人,研究员,研究方向为作物栽培,(E-mail) 作者简介:
杨波(1992-),男,宁夏中卫人,助理农艺师,研究方向为植物保护,(E-mail)752536645@qq.com
基金资助:
CLC Number:
YANG Bo, WANG Hao, CHEN Yongwei, HA Rong, JIN Wei, XU Can, ZHANG Min, YANG Guili, YANG Hongbo, MA Wenli. Study on establishment and optimization of rapid tissue culture propagation technology of three wine grape cultivars[J]. Xinjiang Agricultural Sciences, 2024, 61(1): 109-117.
杨波, 王昊, 陈永伟, 哈蓉, 靳韦, 徐灿, 张敏, 杨桂丽, 杨宏波, 马文礼. 3个酿酒葡萄品种组培快繁体系的建立及优化[J]. 新疆农业科学, 2024, 61(1): 109-117.
处理 编号 Process number | 10%次氯酸钠 消毒时间 10% sodium hypochlorite disinfection time(min) | 75%乙醇 消毒时间 75%Alcohol disinfection time(s) | 0.1%氯化汞 消毒时间 0.1%Mercury chloride disinfection time(min) |
---|---|---|---|
C1 | 5 | 5 | 5 |
C2 | 5 | 30 | 7.5 |
C3 | 5 | 60 | 10 |
C4 | 7.5 | 5 | 7.5 |
C5 | 7.5 | 30 | 10 |
C6 | 7.5 | 60 | 5 |
C7 | 10 | 5 | 10 |
C8 | 10 | 30 | 5 |
C9 | 10 | 60 | 7.5 |
Tab.1 Disinfection treatment of explants of different wine grape
处理 编号 Process number | 10%次氯酸钠 消毒时间 10% sodium hypochlorite disinfection time(min) | 75%乙醇 消毒时间 75%Alcohol disinfection time(s) | 0.1%氯化汞 消毒时间 0.1%Mercury chloride disinfection time(min) |
---|---|---|---|
C1 | 5 | 5 | 5 |
C2 | 5 | 30 | 7.5 |
C3 | 5 | 60 | 10 |
C4 | 7.5 | 5 | 7.5 |
C5 | 7.5 | 30 | 10 |
C6 | 7.5 | 60 | 5 |
C7 | 10 | 5 | 10 |
C8 | 10 | 30 | 5 |
C9 | 10 | 60 | 7.5 |
处理编号 Process number | 培养基类型 Type of culture medium | 6-BA (mg/L) | NAA (mg/L) |
---|---|---|---|
D1 | MS | 0.5 | 0 |
D2 | MS | 1 | 0.05 |
D3 | MS | 1.5 | 0.1 |
D4 | 1/2MS | 0.5 | 0.05 |
D5 | 1/2MS | 1 | 0.1 |
D6 | 1/2MS | 1.5 | 0 |
D7 | GS | 0.5 | 0.1 |
D8 | GS | 1 | 0 |
D9 | GS | 1.5 | 0.05 |
Tab.2 Startup medium for explants of different wine grape varieties
处理编号 Process number | 培养基类型 Type of culture medium | 6-BA (mg/L) | NAA (mg/L) |
---|---|---|---|
D1 | MS | 0.5 | 0 |
D2 | MS | 1 | 0.05 |
D3 | MS | 1.5 | 0.1 |
D4 | 1/2MS | 0.5 | 0.05 |
D5 | 1/2MS | 1 | 0.1 |
D6 | 1/2MS | 1.5 | 0 |
D7 | GS | 0.5 | 0.1 |
D8 | GS | 1 | 0 |
D9 | GS | 1.5 | 0.05 |
处理编号 Process number | 培养基类型 Type of culture medium | 6-BA (mg/L) | IBA (mg/L) |
---|---|---|---|
E1 | MS | 0.5 | 0.1 |
E2 | MS | 1 | 0.2 |
E3 | MS | 1.5 | 0.3 |
E4 | 1/2MS | 0.5 | 0.2 |
E5 | 1/2MS | 1 | 0.3 |
E6 | 1/2MS | 1.5 | 0.1 |
E7 | GS | 0.5 | 0.3 |
E8 | GS | 1 | 0.1 |
E9 | GS | 1.5 | 0.2 |
Tab.3 Selection of subculture media for different wine grape varieties
处理编号 Process number | 培养基类型 Type of culture medium | 6-BA (mg/L) | IBA (mg/L) |
---|---|---|---|
E1 | MS | 0.5 | 0.1 |
E2 | MS | 1 | 0.2 |
E3 | MS | 1.5 | 0.3 |
E4 | 1/2MS | 0.5 | 0.2 |
E5 | 1/2MS | 1 | 0.3 |
E6 | 1/2MS | 1.5 | 0.1 |
E7 | GS | 0.5 | 0.3 |
E8 | GS | 1 | 0.1 |
E9 | GS | 1.5 | 0.2 |
处理编号 Process number | 培养基类型 Type of culture medium | IBA (mg/L) | NAA (mg/L) |
---|---|---|---|
F1 | MS | 0.2 | 0 |
F2 | MS | 0.4 | 0.2 |
F3 | MS | 0.6 | 0.4 |
F4 | 1/2MS | 0.2 | 0.2 |
F5 | 1/2MS | 0.4 | 0.4 |
F6 | 1/2MS | 0.6 | 0 |
F7 | GS | 0.2 | 0.4 |
F8 | GS | 0.4 | 0 |
F9 | GS | 0.6 | 0.2 |
Tab.4 The selection of rooting medium for different wine grape varieties
处理编号 Process number | 培养基类型 Type of culture medium | IBA (mg/L) | NAA (mg/L) |
---|---|---|---|
F1 | MS | 0.2 | 0 |
F2 | MS | 0.4 | 0.2 |
F3 | MS | 0.6 | 0.4 |
F4 | 1/2MS | 0.2 | 0.2 |
F5 | 1/2MS | 0.4 | 0.4 |
F6 | 1/2MS | 0.6 | 0 |
F7 | GS | 0.2 | 0.4 |
F8 | GS | 0.4 | 0 |
F9 | GS | 0.6 | 0.2 |
处理编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | |||
---|---|---|---|---|---|---|
污染率 Pollution rate(%) | 成活率 Survival rate(%) | 污染率 Pollution rate(%) | 成活率 Survival rate(%) | 污染率 Pollution rate(%) | 成活率 Survival rate(%) | |
C1 | 68.75±3.06b | 31.25±0.82b | 38.46±0.78b | 61.54±1.26a | 68.75±2.41c | 31.25±0.61d |
C2 | 81.25±1.02a | 18.75±1.77c | 40.74±0.41b | 59.26±2.82a | 75.00±2.45b | 25.00±1.63e |
C3 | 81.25±2.65a | 18.75±1.22c | 48.00±2.45a | 52.00±1.22b | 62.50±1.63d | 37.50±1.63c |
C4 | 68.75±3.06b | 31.25±1.85b | 50.00±1.63a | 50.00±4.08b | 68.75±2.41c | 31.25±2.24d |
C5 | 67.75±2.24b | 32.25±1.71b | 37.93±1.16b | 62.07±1.28a | 50.00±1.63e | 57.00±1.22a |
C6 | 62.50±2.04c | 37.50±1.67a | 39.29±1.46b | 60.71±1.80a | 75.00±2.45b | 25.00±0.41e |
C7 | 62.50±3.67c | 37.50±1.57a | 38.36±0.09b | 63.64±2.77a | 53.75±18.4e | 56.25±0.86a |
C8 | 68.75±2.24b | 31.25±1.36b | 40.00±1.63b | 60.00±3.26a | 51.00±2.04e | 50.00±1.22b |
C9 | 68.75±3.06b | 31.25±2.24b | 48.28±2.02a | 51.72±1.31b | 81.25±1.43a | 18.75±1.02f |
Tab.5 The effect of different treatments on the survival rate of explants of 3 wine grape varieties
处理编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | |||
---|---|---|---|---|---|---|
污染率 Pollution rate(%) | 成活率 Survival rate(%) | 污染率 Pollution rate(%) | 成活率 Survival rate(%) | 污染率 Pollution rate(%) | 成活率 Survival rate(%) | |
C1 | 68.75±3.06b | 31.25±0.82b | 38.46±0.78b | 61.54±1.26a | 68.75±2.41c | 31.25±0.61d |
C2 | 81.25±1.02a | 18.75±1.77c | 40.74±0.41b | 59.26±2.82a | 75.00±2.45b | 25.00±1.63e |
C3 | 81.25±2.65a | 18.75±1.22c | 48.00±2.45a | 52.00±1.22b | 62.50±1.63d | 37.50±1.63c |
C4 | 68.75±3.06b | 31.25±1.85b | 50.00±1.63a | 50.00±4.08b | 68.75±2.41c | 31.25±2.24d |
C5 | 67.75±2.24b | 32.25±1.71b | 37.93±1.16b | 62.07±1.28a | 50.00±1.63e | 57.00±1.22a |
C6 | 62.50±2.04c | 37.50±1.67a | 39.29±1.46b | 60.71±1.80a | 75.00±2.45b | 25.00±0.41e |
C7 | 62.50±3.67c | 37.50±1.57a | 38.36±0.09b | 63.64±2.77a | 53.75±18.4e | 56.25±0.86a |
C8 | 68.75±2.24b | 31.25±1.36b | 40.00±1.63b | 60.00±3.26a | 51.00±2.04e | 50.00±1.22b |
C9 | 68.75±3.06b | 31.25±2.24b | 48.28±2.02a | 51.72±1.31b | 81.25±1.43a | 18.75±1.02f |
处理编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | |||
---|---|---|---|---|---|---|
生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | 生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | 生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | |
F1 | 66.67±0.06 c | 3.67±0.27 f | 86.66±0.13 a | 5.44±3.20 d | 70.00±0.04 bcd | 10.56±1.50 b |
F2 | 81.67±0.06 ab | 8.67±1.09 e | 88.33±0.13 a | 7.83±1.04 bcd | 75.00±0.07 abcd | 11.22±4.32 b |
F3 | 83.33±0.02 ab | 15.44±1.10 cd | 86.66±0.06 a | 7.50±4.44 bcd | 76.67±0.06 abc | 14.56±1.40 ab |
F4 | 88.33±0.02 a | 18.56±2.04 abc | 83.33±0.03 a | 6.78±2.04 bcd | 80.00±0.04 ab | 16.44±6.95 ab |
F5 | 93.33±0.05 a | 20.11±0.57 a | 93.33±0.08 a | 15.83±6.30 a | 85.00±0.04 a | 21.67±1.52 a |
F6 | 85.00±0.08 ab | 13.56±1.77 d | 80.00±0.10 a | 10.67±5.51 abcd | 75.00±0.04 abcd | 11.78±1.85 b |
F7 | 90.00±0.04 a | 19.00±2.23 ab | 91.67±0.06 a | 12.56±2.87 abc | 81.67±0.02 a | 21.44±6.12 a |
F8 | 75.00±0.04 bc | 8.56±2.22 e | 85.00±0.10 a | 6.22±1.35 cd | 66.67±0.06 cd | 9.11±0.16 b |
F9 | 86.67±0.06 ab | 15.78±0.16 bcd | 90.00±0.09 a | 13.72±1.70 ab | 65.00±0.04 d | 14.00±5.00 ab |
Tab.6 The effects of different treatments various varieties of wine grapes on rooting of various varieties
处理编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | |||
---|---|---|---|---|---|---|
生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | 生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | 生根率 Rooting rate(%) | 平均生根数(条) Average number of root(twig) | |
F1 | 66.67±0.06 c | 3.67±0.27 f | 86.66±0.13 a | 5.44±3.20 d | 70.00±0.04 bcd | 10.56±1.50 b |
F2 | 81.67±0.06 ab | 8.67±1.09 e | 88.33±0.13 a | 7.83±1.04 bcd | 75.00±0.07 abcd | 11.22±4.32 b |
F3 | 83.33±0.02 ab | 15.44±1.10 cd | 86.66±0.06 a | 7.50±4.44 bcd | 76.67±0.06 abc | 14.56±1.40 ab |
F4 | 88.33±0.02 a | 18.56±2.04 abc | 83.33±0.03 a | 6.78±2.04 bcd | 80.00±0.04 ab | 16.44±6.95 ab |
F5 | 93.33±0.05 a | 20.11±0.57 a | 93.33±0.08 a | 15.83±6.30 a | 85.00±0.04 a | 21.67±1.52 a |
F6 | 85.00±0.08 ab | 13.56±1.77 d | 80.00±0.10 a | 10.67±5.51 abcd | 75.00±0.04 abcd | 11.78±1.85 b |
F7 | 90.00±0.04 a | 19.00±2.23 ab | 91.67±0.06 a | 12.56±2.87 abc | 81.67±0.02 a | 21.44±6.12 a |
F8 | 75.00±0.04 bc | 8.56±2.22 e | 85.00±0.10 a | 6.22±1.35 cd | 66.67±0.06 cd | 9.11±0.16 b |
F9 | 86.67±0.06 ab | 15.78±0.16 bcd | 90.00±0.09 a | 13.72±1.70 ab | 65.00±0.04 d | 14.00±5.00 ab |
处理 编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | ||||||
---|---|---|---|---|---|---|---|---|---|
生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | 生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | 生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | |
F1 | 2.71±0.17 g | 5.85±0.08c | 1.03±0.02 a | 4.01±1.12 cd | 8.87±0.04b | 1.47±0.05ab | 4.98±0.16 d | 7.97± 0.31a | 1.09± 0.07b |
F2 | 3.55±0.08 f | 3.91±0.03g | 1.00± 0.02a | 3.20±0.27 cd | 3.19±0.04fg | 1.58±0.31a | 4.63±0.02 e | 5.82± 0.26c | 1.25± 0.10a |
F3 | 5.36±0.09 d | 4.33± 0.09f | 1.01± 0.01a | 3.13±1.03 cd | 3.27± 0.08ef | 1.17±0.05cd | 5.34±0.08 c | 5.28± 0.13d | 0.98±0.08 bc |
F4 | 7.28±0.15 a | 8.78±0.08b | 1.14±0.04 a | 2.62±0.42 d | 2.03± 0.02h | 0.93±0.10de | 6.40±0.08 b | 6.85± 0.24b | 0.93±0.01 c |
F5 | 6.84±0.26 b | 5.39±0.09d | 1.01± 0.01a | 6.34±1.52 a | 7.67±0.06c | 1.35±0.12abc | 7.62±0.14 a | 7.09±0.07 b | 0.99± 0.08bc |
F6 | 6.15±0.04 c | 9.34± 0.09a | 1.10±0.09 a | 5.75±1.17 ab | 11.19±0.07a | 1.25±0.12bc | 5.03±0.02 d | 7.99± 0.11a | 0.89±0.08cd |
F7 | 5.80±0.20 c | 2.41±0.19h | 0.85±0.08b | 4.44±0.86 bc | 3.37± 0.04e | 0.78±0.01e | 6.41±0.11 b | 2.57± 0.31f | 0.76± 0.01de |
F8 | 3.78±0.18 f | 5.07± 0.08e | 0.69±0.13 c | 3.38±0.69 cd | 5.59± 0.15d | 0.88±0.01e | 3.45±0.12 f | 3.36±0.04e | 0.57± 0.02f |
F9 | 4.91±0.27 e | 2.13±0.04 i | 0.69±0.06 c | 4.64±0.40 bc | 3.05± 0.04g | 0.73±0.05e | 4.43±0.25 e | 2.42± 0.16f | 0.64± 0.07ef |
Tab.7 The effects of different treatments on the comprehensive rooting quality of three wine grape varieties
处理 编号 Process number | 紫大夫Dunkelfelder T | 长相思Sauvignon blanc | 小味儿多Petit verdot | ||||||
---|---|---|---|---|---|---|---|---|---|
生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | 生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | 生根综 合质量 Comprehensive quality of rooting | 平均根长 Average root length (cm) | 平均根粗 Average root diameter (mm) | |
F1 | 2.71±0.17 g | 5.85±0.08c | 1.03±0.02 a | 4.01±1.12 cd | 8.87±0.04b | 1.47±0.05ab | 4.98±0.16 d | 7.97± 0.31a | 1.09± 0.07b |
F2 | 3.55±0.08 f | 3.91±0.03g | 1.00± 0.02a | 3.20±0.27 cd | 3.19±0.04fg | 1.58±0.31a | 4.63±0.02 e | 5.82± 0.26c | 1.25± 0.10a |
F3 | 5.36±0.09 d | 4.33± 0.09f | 1.01± 0.01a | 3.13±1.03 cd | 3.27± 0.08ef | 1.17±0.05cd | 5.34±0.08 c | 5.28± 0.13d | 0.98±0.08 bc |
F4 | 7.28±0.15 a | 8.78±0.08b | 1.14±0.04 a | 2.62±0.42 d | 2.03± 0.02h | 0.93±0.10de | 6.40±0.08 b | 6.85± 0.24b | 0.93±0.01 c |
F5 | 6.84±0.26 b | 5.39±0.09d | 1.01± 0.01a | 6.34±1.52 a | 7.67±0.06c | 1.35±0.12abc | 7.62±0.14 a | 7.09±0.07 b | 0.99± 0.08bc |
F6 | 6.15±0.04 c | 9.34± 0.09a | 1.10±0.09 a | 5.75±1.17 ab | 11.19±0.07a | 1.25±0.12bc | 5.03±0.02 d | 7.99± 0.11a | 0.89±0.08cd |
F7 | 5.80±0.20 c | 2.41±0.19h | 0.85±0.08b | 4.44±0.86 bc | 3.37± 0.04e | 0.78±0.01e | 6.41±0.11 b | 2.57± 0.31f | 0.76± 0.01de |
F8 | 3.78±0.18 f | 5.07± 0.08e | 0.69±0.13 c | 3.38±0.69 cd | 5.59± 0.15d | 0.88±0.01e | 3.45±0.12 f | 3.36±0.04e | 0.57± 0.02f |
F9 | 4.91±0.27 e | 2.13±0.04 i | 0.69±0.06 c | 4.64±0.40 bc | 3.05± 0.04g | 0.73±0.05e | 4.43±0.25 e | 2.42± 0.16f | 0.64± 0.07ef |
品种 Varieties | 检测病毒类型The detect virus type | |||||
---|---|---|---|---|---|---|
GLRaV-1 | GLRaV-3 | GLRaV-5 | GVA | GFkV | GFLV | |
黑比诺Pinot noir | — | — | — | + | — | — |
桑娇维塞Sangiovese | — | — | — | + | — | — |
霞多丽Chardonnay | — | — | — | + | — | — |
马尔贝克Malbec | — | — | — | + | — | — |
长相思Sauvignon blanc | — | — | — | + | — | — |
小味儿多Petit verdot | — | — | — | — | — | — |
紫大夫Dunkelfelder T | — | — | — | — | — | — |
Tab.8 The virus detection results of several wine grape varieties
品种 Varieties | 检测病毒类型The detect virus type | |||||
---|---|---|---|---|---|---|
GLRaV-1 | GLRaV-3 | GLRaV-5 | GVA | GFkV | GFLV | |
黑比诺Pinot noir | — | — | — | + | — | — |
桑娇维塞Sangiovese | — | — | — | + | — | — |
霞多丽Chardonnay | — | — | — | + | — | — |
马尔贝克Malbec | — | — | — | + | — | — |
长相思Sauvignon blanc | — | — | — | + | — | — |
小味儿多Petit verdot | — | — | — | — | — | — |
紫大夫Dunkelfelder T | — | — | — | — | — | — |
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