Effects of Low Temperature Stress in Bud Stage on Photosynthetic Characteristics of Cotton

doi:10.6048/j.issn.1001-4330.2022.07.002

Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (7): 1573-1581.DOI: 10.6048/j.issn.1001-4330.2022.07.002

• Crop Genetics and Breeding·Cultivation Physiology·Germplasm Resources • Previous Articles Next Articles

Effects of Low Temperature Stress in Bud Stage on Photosynthetic Characteristics of Cotton

ZHONG Xinnian¹(), LIANG Qigan², LI Youzhong¹^,³(), ZHAO Jianhua¹, MA Haixia¹, LI Zhibo¹()

1. Key laboratory for Oasis Eco-agriculture, Xinjiang Production and Construction Corps/College of Agronomy, Shihezi University,Shihezi Xinjiang 832003,China
2. Hainan Sanya Crop Breeding Test Center of Xinjiang Academy of Agricultural Sciences, Sanya Hainan 572014, China
3. Key Laboratory of Crop Germplasm Innovation and Gene Resource Utilization of XPCC, Biotechnology Research Institute, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi,Xinjiang 832003,China

Received:2021-10-22 Online:2022-07-20 Published:2022-08-04
Correspondence author: LI Youzhong, LI Zhibo
Supported by:
Young and Middle-Aged Leading Talents in Scientific and Technological Innovation Program Project of the 8th Division of XPCC(2020RC06);Open Project of the National Key Laboratory of Cotton Biology(CB2021A09);Special Project of Animal Breeding and Plant Cultivation of Shihezi University(YZZX201602);Key Laboratory Open Project for Oasis Ecological Agriculture of XPCC(201902)

蕾期低温胁迫对棉花光合特性的影响

钟信念¹(), 梁其干², 李有忠¹^,³(), 赵建华¹, 马海霞¹, 李志博¹()

1.石河子大学农学院/新疆兵团绿洲生态农业重点实验室
2.新疆农业科学院/海南三亚农作物育种试验中心,海南三亚 572014
3.新疆农垦科学院生物技术研究所/作物种质创新与基因资源利用兵团重点实验室,新疆石河子 8320032

通讯作者: 李有忠,李志博
作者简介:钟信念(1994-), 女,硕士研究生,海南临高人,研究方向为作物育种与抗逆生理,(E-mail) 2386224134@qq.com
基金资助:
八师石河子市中青年科技创新领军人才计划项目(2020RC06);棉花生物学国家重点实验室开放课题(CB2021A09);石河子大学动植物培育专项(YZZX201602);新疆生产建设兵团绿洲生态农业重点实验室开放课题(201902)

Abstract

Abstract:

【Objective】 To explore the response law and screen the photosynthetic indexes related to cold tolerance of cotton by analyzing the effects of different low temperature stress on photosynthetic characteristics of different cotton varieties in bud stage so as to provide a theoretical basis and technical guidance for identification of cold tolerant resources and breeding of cold tolerant cotton varieties. 【Methods】 Three low temperature gradients (T₁ (20/15℃, day / night), T₂ (15 / 10℃), T₃ (10 / 5℃) and CK (28/20℃) were applied in the bud stage. 【Result】 Net photosynthetic rate (PN), stomatal conductance (GS), transpiration rate (TR) and stomatal limit value (LS) of cotton in bud stage decreased significantly under low temperature stress, intercellular carbon dioxide concentration (CI) decreased first and then increased, and water use efficiency (WUE) increased first and then decreased. 【Conclusion】 The degree of low temperature stress can be judged according to the stomatal conductance (GS), transpiration rate (TR), intercellular carbon dioxide concentration (CI) and water use efficiency (WUE). Under T₁ stress, the decrease of photosynthetic rate of cotton in bud stage is caused by stomatal limiting factors; under T₂ stress, the decrease of photosynthetic rate of cotton in bud stage is mainly caused by stomatal factors first and then by non stomatal factors; under T₃ stress, non stomatal factors are the main factors limiting photosynthesis of cotton in bud stage. Low temperature stress inhibits photosynthesis of leaves in bud stage. The higher the stress intensity, the more obvious the inhibition on cotton growth in bud stage and the longer the duration of low temperature treatment, the more obvious the inhibition on cotton growth at bud stage.

Key words: cotton; low temperature stress; bud stage; photosynthesis

摘要：

【目的】分析蕾期不同低温胁迫对不同棉花品种的光合特性的影响,研究响应规律,筛选蕾期棉花耐冷性相关的光合指标,为耐冷资源鉴定、耐冷棉花品种选育提供依据和技术指导。【方法】材料蕾期设3个低温梯度: T₁(20/15℃,昼/夜)、T₂(15/10℃)、T₃(10/5℃)和对CK(28/20℃)处理。【结果】蕾期棉花在各低温胁迫条件下净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、气孔限制值(LS)明显下降,细胞间二氧化碳浓度(Ci)随着胁迫强度的提升及胁迫时间的延长总体先降后升;水分利用率(WUE)先升后降。【结论】可根据蕾期棉花的气孔导度(Gs)、蒸腾速率(Tr)、细胞间二氧化碳浓度(Ci)、水分利用率(WUE)来判断其受到的低温胁迫程度。T₁胁迫下蕾期棉花光合速率降低是由气孔限制因素导致;T₂胁迫下蕾期棉花光合速率降低的主要原因先为气孔因素后转为非气孔因素;T₃胁迫下非气孔因素是限制蕾期棉花光合作用的主要因素。低温胁迫对蕾期叶片的光合作用有抑制的作用。胁迫强度越大,对蕾期棉花生长的抑制作用越明显,低温处理的时间持续越长,对蕾期棉花生长的抑制作用越明显。

关键词: 棉花, 低温胁迫, 蕾期, 光合作用

CLC Number:

S562

ZHONG Xinnian, LIANG Qigan, LI Youzhong, ZHAO Jianhua, MA Haixia, LI Zhibo. Effects of Low Temperature Stress in Bud Stage on Photosynthetic Characteristics of Cotton[J]. Xinjiang Agricultural Sciences, 2022, 59(7): 1573-1581.

钟信念, 梁其干, 李有忠, 赵建华, 马海霞, 李志博. 蕾期低温胁迫对棉花光合特性的影响[J]. 新疆农业科学, 2022, 59(7): 1573-1581.

Figures/Tables 7

Fig.1 General situation of experimental incubator

Fig.2 Effects of low temperature stress on net photosynthetic rate (Pn)

Fig.3 Effects of low temperature stress on stomatal conductance (Gs)

Fig. 4 Effects of low temperature stress on intercellular CO2 concentration (Ci)

Fig.5 Effect of low temperature stress on transpiration rate (Tr)

Fig.6 Effects of low temperature stress on water use efficiency (WUE)

Fig. 7 Effects of low temperature stress on water use efficiency (WUE)

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Effects of Low Temperature Stress in Bud Stage on Photosynthetic Characteristics of Cotton

蕾期低温胁迫对棉花光合特性的影响

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