Cloning and Functional Analysis of Cytochrome P450 Superfamily Gene GhCYP85A2-1 in Upland Cotton

doi:10.6048/j.issn.1001-4330.2021.02.001

Abstract

Abstract: 【Objective】 The principal objective is to investigate the biological function of CYP85A subfamily genes of the cytochrome P450 superfamily in the development of cotton plant height in the hope of providing a scientific basis for molecular breeding of upland cotton plants.【Method】 A CYP85A family gene GhCYP85A2-1 was cloned from upland cotton by homologous cloning. Tobacco rattle virus (TRV) induced gene silencing technology (Virus-induced gene silencing, VIGS) was used to construct GhCYP85A2-1 gene silencing vector, total RNA was extracted, and gene expression of GhCYP85A2-1 in infecting cotton seedlings was investigated by qRT-PCR.【Result】 The study found that the height of GhCYP85A2-1 gene silenced plants was significantly reduced, and so was the gene expression.【Conclusion】 GhCYP85A2-1 gene plays an important regulatory role in the development of cotton plant height.

Key words: cotton; CYP85A2; plant height; VIGS

摘要： 【目的】研究细胞色素P450超家族CYP85A亚家族基因在棉花株高发育中的生物学功能,为陆地棉株高分子育种提供理论依据和基因资源。【方法】 采用同源克隆方法,从陆地棉中克隆CYP85A家族基因GhCYP85A2-1,利用烟草脆裂病毒(Tobacco rattle virus, TRV)诱导的基因沉默技术(Virus-induced gene silencing, VIGS)构建GhCYP85A2-1基因沉默载体,利用qRT-PCR技术检测侵染棉花幼苗的基因表达量。【结果】 GhCYP85A2-1基因沉默植株的株高显著降低,基因的表达量也显著下降。【结论】 GhCYP85A2-1基因在棉花株高发育过程中起到重要调控作用。

关键词: 棉花, CYP85A2, 株高, 病毒诱导的基因沉默

CLC Number:

S562

ZHANG Yi, SHAO Dongnan, XUE Fei, LI Huqing, WANG Xuefeng, LI Yanjun, ZHANG Xinyu, LIU Feng, SUN Jie. Cloning and Functional Analysis of Cytochrome P450 Superfamily Gene GhCYP85A2-1 in Upland Cotton[J]. Xinjiang Agricultural Sciences, 2021, 58(2): 197-205.

张翼, 邵冬南, 薛飞, 李虎情, 王学峰, 李艳军, 张新宇, 刘峰, 孙杰. 陆地棉细胞色素P450超家族基因GhCYP85A2-1的克隆与功能分析[J]. 新疆农业科学, 2021, 58(2): 197-205.

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