Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection

doi:10.6048/j.issn.1001-4330.2017.11.011

Xinjiang Agricultural Sciences ›› 2017, Vol. 54 ›› Issue (11): 2046-2053.DOI: 10.6048/j.issn.1001-4330.2017.11.011

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Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection

Mayinuer Mijiti, Zumina, Sajida Aizizi, GUO Qing -yuan

Xinjiang Agricultural University / College of Agronomy / Key Laboratory of the Agriculture and Forestry Pest Monitoring and Safety Control of College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, China

Received:2017-11-01 Online:2017-11-20 Published:2017-12-06
Correspondence author: GUO Qing-yuan(1962-),Sichuan people, Professor of college of Agronomy, Xinjiang Agricultural University, working on plant pathology,(E-mail)guoqingyuan3009@sina.com
Supported by:
Special projects of Ministry of Agriculture “Research and integration of crop root rot disease control technology”(201503112);Key research and development plans of the xinjiang uygur autonomous region “Development of new technology and product for control Main diseases and pests and reduce Pesticide and increase effect in cotton field”(2016B01001)

基于分子检测新疆东部棉花根茎部病害种类及其分布

马依努尔·米吉提, 族米娜, 萨吉达·艾孜则, 郭庆元

新疆农业大学/新疆农业大学农学院农林有害生物监测与安全防控重点实验室,乌鲁木齐 830052

通讯作者: 郭庆元(1962-),四川人,教授,硕士生导师,研究方向为植物病理,(E-mail)guoqingyuan3009@sina.com
作者简介:马依努尔·米吉提(1991-),新疆人,硕士研究生,研究方向为植物病理,(E-mail)2517884144@qq.com
基金资助:
农业部专项“作物根腐类病害防控技术研究与集成”(201503112);新疆维吾尔自治区重点研发计划“棉田主要病虫防治及农药减施增效新技术与产品研发”(2016B01001)

Abstract

Abstract: 【Objective】 Clarifying species and distribution of cotton root and stem diseases in eastern Xinjiang can provide a basis for effective prevention and control of such diseases in the local area.【Method】The reported specific primers of the 4 species pathogens (Fusarium oxysporum, Rhizoctonia solani, verticillium dahliae and Fusarium verticilliodes) which cause the root and stem disease of cotton are used for the specific verification, and carrying out PCR detection for the mixed disease samples of the collected according to different villages (townships) from the cotton areas in Eastern Xinjiang.【Result】In the tested system with sensitivity of 0.5 ng/μL, 3 species of 4 pathogenic bacteria were detected from the 22 mixed samples of cotton infected plants from the Eastern Xinjiang. They were respectively Fusarium oxysporum, Rhizoctonia solani and verticillium dahliae;Among them, Fusarium oxysporum had the highest detection rate,Rhizoctonia solani was the second, V. dahliae dad the lower detection rate. But, the detection rate ofFusarium verticilliodes was 0% in the 22 mixed samples.【Conclusion】The detection results indicated that the Fusarium wilt,Rhizoctonia damping-off and Verticillium yellow wilt were the main root and stem diseases of cotton in Eastern Xinjiang; Among which, Fusarium wilt is the most common one, followed by Rhizoctonia damping-off, and the incidence of Verticillium wilt is relatively low. In Eastern Xinjiang, red rot has not occurred or distribute very little.

Key words: cotton; root and stem disease; PCR detection; distribution of disease

摘要： 【目的】研究新疆东部棉花根茎部病害的种类及分布,为当地高效防控此类病害提供依据。【方法】采用已报道的4种引起棉花根茎部病害的主要病原菌(尖孢镰孢霉、立枯丝核菌、大丽轮枝菌和拟轮枝镰孢霉)的特异性引物,特异性验证后,分别对新疆东部棉花区按不同以村(连)为单位采集的混合病样进行PCR检测。【结果】在检测灵敏度为0.5 ng/μL的检测体系中,从22份新疆东部棉花病株混合样品中可以检测出4种病原菌中的3种,分别为尖孢镰孢霉、立枯丝核菌、大丽轮枝菌;其中尖孢镰刀菌检出率最高,立枯丝核菌检出率次之,大丽轮枝菌检出率较低;而拟轮枝镰孢霉在22份混合病样中的检出率0%。【结论】枯萎病、立枯病和黄萎病是新疆东部根茎部主要病害;其中枯萎病的发生最为普遍,立枯病次之,黄萎病发生的普遍率相对较低,红腐病在新疆东部无发生或发生分布极少。

关键词: 棉花, 根茎部病害, PCR检测, 病害分布

CLC Number:

S435.621

Mayinuer Mijiti, Zumina, Sajida Aizizi, GUO Qing -yuan. Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection[J]. Xinjiang Agricultural Sciences, 2017, 54(11): 2046-2053.

马依努尔·米吉提, 族米娜, 萨吉达·艾孜则, 郭庆元. 基于分子检测新疆东部棉花根茎部病害种类及其分布[J]. 新疆农业科学, 2017, 54(11): 2046-2053.

References 15

[1]	戴小枫,郭予元. 棉铃虫的抗药性与治理[M]. 北京:中国农业出版社,1995.DAI Xiao-feng,GUO Yu-yuan.(1995). Resistance and treatment of Cotton bollworm [M]. Beijing: China Agriculture Press. (in Chinese)
[2]	陈其煐. 我国棉花病害发生情况及防治进展[J]. 农药,1996,(3):6-9,11.CHEN Qi-ying.(1996). Cotton diseases and progresses of their Control in China [J]. Agrochemicals,(3):6-9,11.(in Chinese)
[3]	郭淑丽. 棉花枯萎病和黄萎病的综合防治[J]. 科学种养,2008,(9):28. GUO Shu-li.(2008). Comprehensive control of cotton wilt and verticillium wilt [J]. Scientific farming, (9):28.(in Chinese)
[4]	孜乃提古丽·阿不都热西提. 棉花棉苗立枯病的发生及防治[J]. 现代农业科技,2009,(11):131-132.Zinaitiguli·Abudourexiti.(2009). Occurrence and prevention of the Rhizoctonia damping-off of cotton [J]. Modern Agricultural Science and Technology,(11):131-132. (in Chinese)
[5]	王今. 棉花病害症状识别与化学用药[J]. 农技服务,2011,(7):990-991.WANG Jin .(2011). Symptom identification and chemical medicine of cotton disease [J]. Agricultural Technology Service, (7):990-991. (in Chinese)
[6]	潘月敏. 棉花红腐病菌生理生化及有效复配剂筛选研究[D]. 合肥: 安徽农业大学硕士学位论文,2005.PAN Yue-min. (2005). On physiological and biochemical characteristics of Fusarium moniliforme and screening for effective mixed preparations [D]. Master Thesis. Anhui Agricultural University, Hefei.(in Chinese)
[7]	周小燕. 棉花病害诊断专家系统研究[D]. 北京:中国农业大学硕士学位论文,2005.ZHOU Xiao-yan. (2005). An expert system for cotton diseases diagnosis [D].C Master Thesis.hina Agricultural University, Beijing.(in Chinese)
[8]	陈文霞. 新疆棉花枯萎病菌遗传多样性研究及其分子检测[D]. 石河子:石河子大学硕士学位论文,2008.CHEN Wen-xia.(2008). Studies on genetic diversity and Molecular detection of Fusarium oxysporum f. sp. hasinfectum in Xinjiang [D]. Master Thesis. Shihezi University, Shihezi.(in Chinese)
[9]	沈其益. 棉花病害一基础研究与防治[M]. 北京:科学出版社,1992.SHEN Qi-yi. (1992). Cotton diseases:Basic research and controlling [M]. Beijing: Science Press.(in Chinese)
[10]	刘萍花,方敦煌,吴祖建. 应用多重PCR检测烟草黑胫病菌、根黑腐病菌、猝倒病菌及立枯病[J].福建农林大学学报(自然科学版),2015,(4):345-349. LIU Peng-hua, FANG Dun-huang, WU Zu-jian.(2015). Simultaneous detection of Phytophthora uicotiauae,Thielaviois basicola,Pythium aphauidermatum and Rhizoctouia solani infected tobacco by multiplex PCR [J]. Journal of Fujian Agriculture and Forestry University (Natural Science Edition),(4):345-349.(in Chinese)
[11]	Klittich, C. J. R., & Leslie, J. F. (1989). Chlorate-resistant, nitrate-utilizing (crn) mutants of fusarium moniliforme (gibberella fujikuroi). Microbiology,135(3): 721-727.
[12]	Sidhu, G. S. (1984). Genetics of gibberella fujikuroi v. spore killer alleles in g. fujikuroi. Journal of Heredity, 75(3):237-238.
[13]	Murillo, I., Cavallarin, L., & Segundo, B. S. (1998). The development of a rapid pcr assay for detection of fusarium moniliforme. European Journal of Plant Pathology, 104(3): 301-311.
[14]	庞莉. 棉花黄萎病菌的快速分子检测技术研究[D]. 北京:中国农业科学院硕士学位论文,2015.PANG Li. (2015). Study on rapid molecular detection technique of Verticillium dahliae of Cotton [D]. Master Thesis. Chinese Academy of Agricultural Sciences, Beijing.(in Chinese)
[15]	赵宗峰.新疆呼图壁县种用苜蓿主要真菌病害及其病原菌初步研究[D]. 乌鲁木齐: 新疆农业大学硕士学位论文,2011.ZHAO Zong-feng.(2011). Preliminary study on major fungal diseases and pathogens of alfalfa in Hutubi Country of Xinjiang [D]. Master Thesis. Xinjiang Agricultural University, Urumqi.(in Chinese)

Species and Distribution of Cotton Root and Stem Diseases in Eastern Xinjiang Based on Molecular Detection

基于分子检测新疆东部棉花根茎部病害种类及其分布

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