Effects of Bio-organic Fertilizer on Yield and Quality of Lycium barbarum L. and Physicochemical Properties of Soil

doi:10.6048/j.issn.1001-4330.2020.03.019

Abstract

Abstract: 【Objective】 In order to compare the effects of different fertilization on the yield and quality of Lycium barbarum L. and physicochemical properties of soil, a field experiment was conducted. 【Method】 Four treatments including chemical fertilizer(CK), 50% chemical fertilizer combined with organic fertilizer(T1), 50% chemical fertilizer combined with S13 bio-organic fertilizer and 50% chemical fertilizer combined with NCD-2 bio-organic fertilizer(T3) were carried out. The yield and quality of Lycium barbarum L. and physicochemical properties as well as microbial population and diversity of soil were analyzed.【Result】 The results showed that after applying bio-organic fertilizer, the contents of soluble solid, 100-grain weight, polysaccharide and total carbohydratewere were increased by 9.6%-9.4%, 10.5%-20.2%, 29.8%-50% and 41.8%-60.2%, respectively and the pH value was decreased by 7.2%-8.6%; The content of soil organic matter was increased by 26.8%-31.8%, and the content of available NPK was increased by 27.7%-41.6%, 26.3%-34.2% and 26.9%-33.3%, respectively; The number of bacteria increased and the number of fungi decreased significantly.【Conclusion】 This study found that 50% chemical fertilizer combined with bio-organic fertilizer had no significant effect on the yield of Lycium barbarum L., but could improve the quality of Lycium barbarum L. and soil nutrients significantly, and optimize the microbial community structure of soil obviously.

Key words: Lycium barbarum Lbio-organic fertilizer; yield; quality; properties of soil

摘要： 【目的】比较化肥、有机肥和生物有机肥对枸杞产量、品质及土壤性状的影响,为改善枸杞品质及提升枸杞园土壤肥力提供科学依据。【方法】设置化肥、50%化肥配施有机肥和50%化肥配施不同生物有机肥共4个处理,测定化肥减施条件下配施生物有机肥对枸杞产量、品质及土壤性状的影响。【结果】施用生物有机肥后,枸杞产量比对照增加3.6%~7.4%,差异不显著;枸杞可溶性固形物、百粒重、枸杞多糖、总糖含量分别提高9.6%~9.4%、10.5%~20.2%、29.8%~50%、41.8%~60.2%;土壤pH值降低7.2%~8.6%,有机质含量提高26.8%~31.8%,速效氮磷钾分别提高27.7%~41.6%、26.3%~34.2%、26.9%~33.3%;细菌数量显著上升,真菌数量显著下降。【结论】化肥减施条件下,配施生物有机肥对枸杞产量无显著影响,但可显著提升枸杞品质和土壤养分,并可明显优化土壤微生物种群结构。

关键词: 枸杞, 生物有机肥, 产量, 品质, 土壤性状

CLC Number:

S663.9

BAO Huifang, WANG Ning, HOU Min, CHEN Jing, WANG Xiaowu, HOU Xinqiang, YANG Rong, CUI Weidong, YANG Wenqi, LONG Xuanqi, ZHAN Faqiang. Effects of Bio-organic Fertilizer on Yield and Quality of Lycium barbarum L. and Physicochemical Properties of Soil[J]. Xinjiang Agricultural Sciences, 2020, 57(3): 545-522.

包慧芳, 王宁, 侯敏, 陈竞, 王小武, 侯新强, 杨蓉, 崔卫东, 杨文琦, 龙宣杞, 詹发强. 生物有机肥对枸杞产量、品质及土壤性状的影响[J]. 新疆农业科学, 2020, 57(3): 545-522.

References

[1] 韩宏伟, 王建友, 李勇, 等. 沙壤土条件下不同施肥水平对成龄枸杞生长及产量的影响[J].中南林业科技大学学报, 2015, 35(4): 46-50.
HAN Hongwei, WANG Jianyou, LI Yong, et al. Effects of different fertilization levels to growth and yield of mature Lycium barbarum in sandy loam condition [J]. Journal of Central South University of Forestry and Technology, 2015, 35(4): 46-50.
[2] 李小刚, 周倩倩, 李捷, 等. 2种微生物肥料对景电灌区枸杞产量及品质的影响[J].四川农业大学学报, 2013,31(2):163-168.
LI Xiaogang, ZHOU Qianqian, LI Jie, et al. Effects of two microbial fertilizers on the growth and quality of Wolfberry (Lycium barbaruml.) in the Jingtai electric-irrigated area [J]. Journal of Sichuan Agricultural University, 2013, 31(2):163-168.
[3] 张晓娜, 李捷, 葛晶, 等. 2种微生物有机肥对枸杞叶绿素荧光参数变化的影响[J].四川农业大学学报, 2014, 32(4):388-392.
ZHANG Xiaona, LI Jie, GE Jing, et al. Effects of two microbial fertilizers on chlorophyll parameters of Lycium barbarum [J]. Journal of Sichuan Agricultural University, 2014, 32(4):388-392.
[4] 侯会静, 韩正砥, 杨雅琴, 等. 生物有机肥的应用及其农田环境效应研究进展[J].中国农学通报, 2019, 35(14):82-88.
HOU Huijing, HAN Zhengdi, YANG Yaqin, et al. Bio-organic fertilizer: application and farmland environmental effects [J]. Chinese Agricultural Science Bulletin, 2019, 35(14):82-88.
[5] 罗青, 李晓莺, 何军, 等. NutriSmart生态型肥料对枸杞产量与品质的影响[J]. 北方园艺, 2007, (9): 39-40.
LUO Qing, LI Xiaoying, HE Jun, et al. Preliminary study on effect of the eco-fertilizer NutriSmart on the yield and quality of Lycium barbarum L.[J]. Northern Horticulture, 2007, (9):39-40.
[6] 王春兰, 谭建萍, 马明呈, 等. 不同施肥处理对枸杞生长及产量、品质的影响[J].青海农林科技, 2013, (3): 1-6.
WANG Chunlan, TAN Jianping, MA Mingcheng, et al. Different Fertilizer Treatments on the Growth Yield and Quality of Lycium barbarum L.[J]. Science and Technology of Qinghai Agriculture and Forestry, 2014,(3): 1-6.
[7] 赵栋, 王有科, 李杰, 等. 4种微生物肥对枸杞生长及抗病性的影响[J].甘肃农业大学学报, 2012, 47(6):87-92.
ZHAO Dong, WANG Youke, LI Jie, et al. Effects of four kinds of microbial fertilizer on growth and resistance of Lycium barbarum[J]. Journal of Gansu Agriculture University, 2012, 47(6):87-92.
[8] Etesami H , Maheshwari D K . Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects[J]. Ecotoxicology & Environmental Safety, 2018, 156:225-246.
[9] Nadeem S M , Ahmad M , Zahir Z A , et al. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments [J]. Biotechnology Advances, 2014, 32(2):429-448.
[10] 唐宇, 包慧芳, 詹发强, 等. 化肥减施条件下配施生物有机肥对番茄生长及品质的影响[J].新疆农业科学, 2019, 56(5): 841-854.
TANG Yu, BAO Huifang, ZHAN Faqiang, et al. Effect of combined application of bio-organic fertilizer on tomato growth and quality under the condition of chemical fertilizer reduction [J]. Xinjiang Agricultural Sciences, 2019, 56(5): 841-854.
[11] 杨晓云, 陈志谊, 蒋盼盼, 等. 解淀粉芽孢杆菌B1619对番茄的促生作用[J].中国生物防治学报, 2016, 32(3): 349-356.
YANG Xiaoyun, CHEN Zhiyi, JIANG Panpan, et al. Growth-promotion effect of Bacillus amyloliquefaciens B1619 on tomato plant [J]. Chinese Journal of Biological Control, 2016, 32(3): 349-356.
[12] 詹发强, 侯敏, 杨蓉, 等.一株番茄枯萎病生防菌的鉴定、定殖与盆栽防效研究[J].新疆农业科学, 2013, 50(7):1277-1287.
ZHANG Faqiang, HOU Min, YANG Rong, et al. Identification, colonization and control effect of an antagonistic bacterium against Fusarium wilt Tomato [J]. Xinjiang Agricultural Sciences, 2013, 50(7): 1277-1287.
[13] 董伟欣, 李宝庆, 李社增, 等. 脂肽类抗生素fengycin在枯草芽孢杆菌NCD-2菌株抑制番茄灰霉病菌中的功能分析[J].植物病理学报, 2013, 43(4): 401-410.
DONG Weixin, LI Baoqing, LI Shezeng, et al. The fengycin lipopeptides are major components in the Bacillus subtilis strain NCD-2 against the growth of Botrytis cinerea [J]. Acta Phatopathologica Sinica, 2013, 43(4): 401-410.
[14] 赵满兴, 刘慧, 白二磊, 等. 腐殖酸和生物有机肥替代化肥对烤烟生长及品质的影响[J].分子植物育种, 2019, 17(9): 3105-3114.
ZHAO Manxing, LIU Hui, BAI Erlei, et al. Effect of different proportion of bio-organic fertilizer and humic acid fertilizer on tobacco growth and leaf quality [J]. Molecular Plant Breeding, 2019, 17(9): 3105-3114.
[15] 孔涛, 马瑜, 刘民, 等. 生物有机肥对土壤养分和土壤微生物的影响[J].干旱区研究, 2016, 33(4): 884-891.
KONG Tao, MA Yu, LIU Min, et al. Effect of applying biological organic fertilizer on soil nutrients and soil Microbes[J]. Arid Zone Research, 2016, 33(4): 884-891.
[16] 郭莹, 王一明, 巫攀, 等.长期施用粪肥对水稻土中微生物群落功能多样性的影响[J].应用与环境生物学报, 2019, 25(3): 593-602.
GUO Ying, WANG Yimin., WU Pan, et al. Influence of long-term manure application in paddy soil on the functional diversity of microbial community[J]. Chinese Journal of Applied and Environmental Biology, 2019, 25(3): 593-602.
[17] 姜莉莉, 王开运, 武玉国, 等. 含高效固氮解淀粉芽孢杆菌生物有机肥在草莓上的应用效果[J].北方园艺, 2018,(20):7-12.
JIANG Lili, WANG Kaiyun, WU Yuguo, et al. Application effectiveness of birorganic fertilizer containing efficient nitrogen fixing Bacillus amyloliquef aciens on strawberry [J]. Northern Horticulture, 2018, (20):7-12.
[18] 卢云峰, 乔策策, 许航, 等. 含Bacillus amyloliquefaciens SQR9“全元”复合微生物液体肥料研制及其促生效果研究[J].土壤通报, 2018, 49(5): 1150-1156.
LU Yunfeng, QIAO Cece., XU Hang, et al. Development of compound microbial liquid fertilizer containing Bacillus amyloliquefaciens SQR9 and its plant growth promotion effect[J]. Chinese Journal of Soil Science, 2018, 49(5): 1150-1156.
[19] 马世震, 彭炳成, 董琪, 等. 生态专用肥在枸杞生产上的应用[J].西北农业学报, 2014, 23(8): 149-154.
MA Shizhen, PENG Bingcheng, DONG Qi, et al. Applicttion of special fertilizer to thg production of Lycium barbarrum L.[J]. Acta Agriculurae Boreali-occidentalis Sinica, 2014, 23(8):149-154.
[20] 邵丽, 谷洁, 高华, 等. 生物复混肥对土壤微生物群落功能多样性的影响[J].西北农业学报, 2012,21(7): 185-189.
SHAO Li, GU Jie, GAO Hua, et al. Effects of Bio-compound fertilizer on soil microbial community functional diversity [J]. Acta Agriculturae Boreali-occidentalis Sinica, 2012, 21(7): 185-189.
[21] 沈仁芳, 赵学强. 土壤微生物在植物获得养分中的作用[J].生态学报, 2015, 35(20):6584-6591.
SHEN Renfang, ZHAO Xueqiang. Role of soil microbes in the acquisition of nutrients by plants [J].Acta Ecologica Sinica, 2015, 35(20): 6584-6591.
[22] Cayo Ramos, Lars M lbak, S ren Molin. Bacterial Activity in the Rhizosphere Analyzed at the Single-Cell Level by Monitoring Ribosome Contents and Synthesis Rates [J]. Applied and Environmental Microbiology, 2000, 66(2): 801–809.
[23] 张楠, 吴凯, 沈怡斐, 等. 根际益生菌解淀粉芽孢杆菌SQR9在香蕉根表的定殖行为研究[J].南京农业大学学报, 2014,37(6) : 59-65.
ZHANG Nan, WU Kai, SHEN Yifei, et al. Investigation of the colonization patterns of plant growth-promoting rhizobacteria Bacillus amyloliquefaciens SQR9 on banana roots [J]. Journal of Nanjing Agricultural University, 2014, 37(6): 59-65.
[24] 韩丽珍, 刘畅, 周静. 接种促生菌对花生根际土壤微生物及营养元素的影响[J].基因组学与应用生物学, 2019, 38(7):3065-3073.
HAN Lizhen, LIU Chan, ZHOU Jing. Effects of inoculation with growth-promoting bacteria on peanut rhizosphere soil microorganism and nutrient elements [J]. Genomics and Applied Biology, 2019, 38(7): 3065-3073.
[25] 钟书堂, 沈宗专, 孙逸飞, 等. 生物有机肥对连作蕉园香蕉生产和土壤可培养微生物区系的影响[J].应用生态学报, 2015, 26(2): 481-489.
ZHONG Shutang, SHEN Zongzhuan, SUN Yifei, et al. Effects of continuous application of bio-organic fertilizer on banana production and cultural microflora of bulk soil in orchard with serious disease incidence [J]. Chinese Journal of Applied Ecology, 2015, 26(2): 481-489.
[26] 常文智, 马鸣超, 陈慧君, 等. 胶质类芽孢杆菌对花生生长和土壤微生物学性状的影响[J].应用与环境生物学报, 2014, 20(2): 185-191.
CHANG Wenzhi, MA Mingchao, CHEN Huijun, et al. Effects of Paenibacillus mucilaginosus on peanut growth and soil microbiological characteristics [J]. Chinese Journal of Applied and Environmental Biology, 2014, 20(2): 185-191.