Xinjiang Agricultural Sciences ›› 2023, Vol. 60 ›› Issue (6): 1460-1465.DOI: 10.6048/j.issn.1001-4330.2023.06.019
• Microbes·Horticultural Special Local Products·Storage and Preservation Processing • Previous Articles Next Articles
LIU Jiangna(), ZHANG Xiying, LI Rongxia, ZHANG Xiaowei, BAI Yunfeng, ZHANG Aiping()
Received:
2022-10-07
Online:
2023-06-20
Published:
2023-06-20
Correspondence author:
ZHANG Aiping(1968-), female, bachelor, researcher, mainly engaged in plant molecular breeding, (E-mail) 592354963@qq.com
Supported by:
刘江娜(), 张西英, 李荣霞, 张小伟, 白云凤, 张爱萍()
通讯作者:
张爱萍(1968-),女,安徽人,研究员,研究方向为植物分子育种,(E-mail) 592354963@qq.com
作者简介:
刘江娜(1985-),女,河北人,硕士,副研究员,研究方向为植物分子育种,(E-mail)2849847373@qq.com
基金资助:
CLC Number:
LIU Jiangna, ZHANG Xiying, LI Rongxia, ZHANG Xiaowei, BAI Yunfeng, ZHANG Aiping. Molecular characteristics and promoter analysis of SlLCY-B2 gene in tomato[J]. Xinjiang Agricultural Sciences, 2023, 60(6): 1460-1465.
刘江娜, 张西英, 李荣霞, 张小伟, 白云凤, 张爱萍. 番茄SlLCY-B2及其启动子的分子特征和sgRNA分析[J]. 新疆农业科学, 2023, 60(6): 1460-1465.
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Fig.1 The digital expression profile of SlLCY-B2 baded on RNA-sequence Note: 1.TA496 fruit, ovary; 2.TA496 fruit,developing/immature green; 3.TA496 fruit, mature green; 4.TA496 fruit, breaker 5.TA496 fruit, red ripe; 6.TA496 flower, 0-3 mm buds; 7.TA496 flower, 3-8 mm buds; 8.TA496 flower, 8 mm-preanthesis buds; 9.TA496 open flower; 10.TA496 flower, a mixture of developmental stages (mixed flower); 11.TA56 wild tomato pollen; 12.R11-13 (Rio Grande x Money Maker) leaf, Pseudomonas susceptible; 13.R11-12 (35S::Pto in Rio Grande x Money Maker) leaf, Pseudomonas resistant; 14.Rio Grande PtoR leaf, mixed elicitor; 15. TA496 shoot/meristem, 4-6 week old plants; 16.TA496 shoot meristem, 8 week old plants; 17.TA496 root, plant at pre-anthesis; 18.TA496 root, plant at fruit set; 19.TA496 root, nutrient deficient; 20.TA496 radicle, seedlings 5 days post-imbibition; 21.TA496 whole seedling,7 days post imbibtion; 22.TA496 quiescent seed; 23.TA496 callus; 24.TA496, E6203 Suspension culture; 25.TA496 crown gall. PRKM: Reads Per Kilo bases per Million reads
Fig.3 The position of sgRNAs in SlLCY-B2 Note:Green shows these sgRNAs located in exon green seed sequence on the whole tomato genome; blue shows the seed sequence of the sgRNA adjacent PAM 12 nt has a matching sequence at one site of other chromosomes; grey indicates these sgRNAs across two exon neighbors or containing continuous TTTT, using pol III promoter to avoid these sgRNA sequences
元件 Elements | 序列及位置 Sequences and position | 数量 Number | 功能 Function |
---|---|---|---|
A-box | CCGTCC: -598 | 1 | 顺式调控元件 |
GATA-motif | AAGGATAAGG: -1048 | 1 | 部分光响应元件 |
GT1-motif | GGTTAAT: - 27, | 1 | 光响应元件 |
GT1-motif | GGTTAA: - 27, | 1 | 光响应元件 |
I-box | atGATAAGGTC: -1046 | 1 | 部分光响应元件 |
LTR | CCGAAA : -378,-707 | 2 | 低温响应顺式调控元件 |
MBS | CAACTG:- 439 | 1 | 与干旱诱导相关的 MYB 结合位点 |
P-box | CCTTTTG:-914,-1346 | 2 | 赤霉素响应元件 |
P-box | CAACAAACCCCTT: -1290 | 1 | 赤霉素响应元件和部分光响应元件 |
TC-rich repeats | ATTCTCTAAC: -1402 | 1 | 防御和胁迫响应顺式调控元件 |
TGA-element | AACGAC:-990 | 1 | 生长素响应元件 |
chs-CMA2a | TCACTTGA: -759 | 1 | 部分光响应元件 |
Circadian | CAAAGATATC: -289 | 1 | 昼夜节律控制顺式调控元件 |
Tab.1 Predicted cis-regulatory elements in SlLCY-B2 promoter
元件 Elements | 序列及位置 Sequences and position | 数量 Number | 功能 Function |
---|---|---|---|
A-box | CCGTCC: -598 | 1 | 顺式调控元件 |
GATA-motif | AAGGATAAGG: -1048 | 1 | 部分光响应元件 |
GT1-motif | GGTTAAT: - 27, | 1 | 光响应元件 |
GT1-motif | GGTTAA: - 27, | 1 | 光响应元件 |
I-box | atGATAAGGTC: -1046 | 1 | 部分光响应元件 |
LTR | CCGAAA : -378,-707 | 2 | 低温响应顺式调控元件 |
MBS | CAACTG:- 439 | 1 | 与干旱诱导相关的 MYB 结合位点 |
P-box | CCTTTTG:-914,-1346 | 2 | 赤霉素响应元件 |
P-box | CAACAAACCCCTT: -1290 | 1 | 赤霉素响应元件和部分光响应元件 |
TC-rich repeats | ATTCTCTAAC: -1402 | 1 | 防御和胁迫响应顺式调控元件 |
TGA-element | AACGAC:-990 | 1 | 生长素响应元件 |
chs-CMA2a | TCACTTGA: -759 | 1 | 部分光响应元件 |
Circadian | CAAAGATATC: -289 | 1 | 昼夜节律控制顺式调控元件 |
Fig.4 The position of sgRNAs and cis-element in SlLCY-B2 promoter Note: sgRNA: Green shows these sgRNAs located in exon green seed sequence on the whole tomato genome; blue shows the seed sequence of the sgRNA adjacent PAM 12 nt has a matching sequence at one site of other chromosomes; grey indicates these sgRNAs across two exon neighbors or containing continuous TTTT, using pol III promoter to avoid these sgRNA sequences. Cis action element: red underscore element is located in the positive chain and blue underscore element in the negative chain
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