Xinjiang Agricultural Sciences ›› 2022, Vol. 59 ›› Issue (7): 1726-1733.DOI: 10.6048/j.issn.1001-4330.2022.07.019
• Plant Protection • Previous Articles Next Articles
ZHANG Luhui(), ZHAO Zhiqiang, GUO Qingyuan()
Received:
2021-09-18
Online:
2022-07-20
Published:
2022-08-04
Correspondence author:
GUO Qingyuan
Supported by:
通讯作者:
郭庆元
作者简介:
张卢慧(1995-),女,新疆博乐人,硕士研究生,研究方向为作物病害及防治,(E-mail) 1421023812@qq.com
基金资助:
CLC Number:
ZHANG Luhui, ZHAO Zhiqiang, GUO Qingyuan. Identification of Pathogen Causing Fruit Bacterial Streak on Pepper[J]. Xinjiang Agricultural Sciences, 2022, 59(7): 1726-1733.
张卢慧, 赵志强, 郭庆元. 辣椒细菌性果实条斑病病原鉴定[J]. 新疆农业科学, 2022, 59(7): 1726-1733.
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URL: http://www.xjnykx.com/EN/10.6048/j.issn.1001-4330.2022.07.019
Fig.1 Symptoms of Bacterial Leaf Spot of Pepper in the Field and Back to symptoms and tobacco allergic reaction results Note: a, b: Symptoms of surface disease on pepper under natural infestation in the field; c:Symptoms of tieback; d: control; e: Strain tobacco allergic reaction
Fig.2 Morphological characteristics of the representative isolate KHL1 straina Note: a: The morphology of the colony on NA medium; b: Gram stain results;C: Size of bacteria
项目 Items | 菌株 Strains | 项目 Items | 菌株 Strains | ||||
---|---|---|---|---|---|---|---|
1-4 | 5 | 6 | 1-4 | 5 | 6 | ||
革兰氏染色Gram stain | - | - | - | 海藻糖Trehalose | + | + | + |
氧化酶Oxidase | + | + | + | 半乳糖Galactose | + | + | - |
接触酶Hydrogen peroxidase | + | + | + | 麦芽糖Maltose | + | + | - |
硝酸盐还原Nitratreduction | + | + | - | 阿拉伯糖Arabinose | + | + | + |
甲基红测定Methylred test | - | ND | - | 纤维二糖Cellobiose | - | - | - |
V-P 测定VogesProskauer | - | ND | - | 菊糖Inulin | - | - | - |
吲哚Indole | - | - | - | 肌醇Inositol | - | - | + |
明胶液化Gelatinliquefaction | - | - | - | 木糖xylose | - | - | - |
过氧化氢酶Catalase | + | + | + | 蔗糖Sucrose | + | - | + |
4度生长Under 4℃ growth | - | - | - | 山梨醇Inositol | - | - | - |
D-果糖 D-fructose | + | + | + | 赤藓糖醇Meso-erythritol | - | - | - |
葡萄糖Glucose | + | + | + | 甘露醇Mannitol | + | + | - |
Table 1Phys iological and biochemical characteristics of 4 strains tested
项目 Items | 菌株 Strains | 项目 Items | 菌株 Strains | ||||
---|---|---|---|---|---|---|---|
1-4 | 5 | 6 | 1-4 | 5 | 6 | ||
革兰氏染色Gram stain | - | - | - | 海藻糖Trehalose | + | + | + |
氧化酶Oxidase | + | + | + | 半乳糖Galactose | + | + | - |
接触酶Hydrogen peroxidase | + | + | + | 麦芽糖Maltose | + | + | - |
硝酸盐还原Nitratreduction | + | + | - | 阿拉伯糖Arabinose | + | + | + |
甲基红测定Methylred test | - | ND | - | 纤维二糖Cellobiose | - | - | - |
V-P 测定VogesProskauer | - | ND | - | 菊糖Inulin | - | - | - |
吲哚Indole | - | - | - | 肌醇Inositol | - | - | + |
明胶液化Gelatinliquefaction | - | - | - | 木糖xylose | - | - | - |
过氧化氢酶Catalase | + | + | + | 蔗糖Sucrose | + | - | + |
4度生长Under 4℃ growth | - | - | - | 山梨醇Inositol | - | - | - |
D-果糖 D-fructose | + | + | + | 赤藓糖醇Meso-erythritol | - | - | - |
葡萄糖Glucose | + | + | + | 甘露醇Mannitol | + | + | - |
Fig.3 The results of 16SrDNA amplification of strains KHL1、KHL4、KHL6、KHL9 Note: M: 2000 D Ladder marker; 1-4:16SrDNA amplification results of strains KHL1、 KHL4、KHL6、 KHL9; CK: negative control
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