KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars (Lines)

doi:10.6048/j.issn.1001-4330.2022.04.001

Abstract

Abstract:

【Objective】 This project aims to study the distribution of functional genes for disease resistance of different wheat varieties (lines) and clarify the in the hope of providing theoretical basis for wheat disease resistance breeding. 【Method】 458 wheat varieties (lines) were detected by KASP through one powdery mildew resistance molecular marker (Pm21), one stripe rust resistance molecular marker (Yr15) and two leaf rust resistance molecular markers (Lr14a and Lr68). 【Result】 There were 2 Pm21 carried lines, 14 Yr15 carried lines, 22 Lr14a carried lines and 48 Lr68 carried lines in 458 wheat cultivars (lines). 【Conclusion】 The distribution frequency of resistance genes in Xinjiang wheat varieties (lines) was about 5% and the 86 lines selected can be used as the parents for resistance breeding of wheat in Xinjiang.

Key words: wheat; disease resistance gene; KASP; marker assays

摘要：

【目的】研究不同小麦品种(系)抗病性状功能基因的分布状态,分析小麦品种(系)的抗病功能基因,为小麦抗病育种提供理论依据。【方法】利用KASP技术通过1个抗白粉病分子标记(Pm21)、1个抗条锈病分子标记(Yr15)和2个抗叶锈病分子标记(Lr14、Lr68),检测458份小麦品种(系)。【结果】筛选出携带Pm21标记材料2份,Yr15标记材料14份,Lr14a标记材料22份,Lr68标记材料48份。【结论】新疆小麦品种(系)中抗病基因的分布频率较低,约为5%。筛选出的86份材料可以作为新疆小麦抗病育种的亲本材料加以应用。

关键词: 小麦, 抗病基因, KASP, 标记检测

CLC Number:

S512

WANG Zhong, ZHANG Hongzhi, GAO Xin, SHI Jia, WANG Lihong, LI Jianfeng, FAN Zheru, ZHAO Qi, ZHANG Yueqiang. KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars (Lines)[J]. Xinjiang Agricultural Sciences, 2022, 59(4): 781-786.

王重, 张宏芝, 高新, 时佳, 王立红, 李剑峰, 樊哲儒, 赵奇, 张跃强. 458份小麦品种(系)抗病性状功能基因的KASP标记检测[J]. 新疆农业科学, 2022, 59(4): 781-786.

Figures/Tables 5

References 17

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抗病功能基因 Disease resistance gene	不同小麦类型 Different wheat types		不同来源 Different sources			合计 Total
抗病功能基因 Disease resistance gene	冬小麦 Winter wheat	春小麦 Spring wheat	审定品种 Varieties	高代品系 Lines	引进种质资源 Introduced variety	合计 Total
Pm21	2(0.44%)	0	2(0.44%)	0	0	2(0.44%)
Yr15	3(0.66%)	11(2.40%)	7(1.53%)	6(1.31%)	1(0.22%)	14(3.06%)
Lr14a	20(4.37%)	2(0.44%)	21(4.59%)	0	1(0.22%)	22(4.80%)
Lr68	2(0.44%)	46(10.04%)	4(0.87%)	15(3.28%)	29(6.33%)	48(10.48%)

抗病功能基因 Disease resistance gene	不同小麦类型 Different wheat types		不同来源 Different sources			合计 Total
抗病功能基因 Disease resistance gene	冬小麦 Winter wheat	春小麦 Spring wheat	审定品种 Varieties	高代品系 Lines	引进种质资源 Introduced variety	合计 Total
Pm21	2(0.44%)	0	2(0.44%)	0	0	2(0.44%)
Yr15	3(0.66%)	11(2.40%)	7(1.53%)	6(1.31%)	1(0.22%)	14(3.06%)
Lr14a	20(4.37%)	2(0.44%)	21(4.59%)	0	1(0.22%)	22(4.80%)
Lr68	2(0.44%)	46(10.04%)	4(0.87%)	15(3.28%)	29(6.33%)	48(10.48%)