新疆农业科学 ›› 2023, Vol. 60 ›› Issue (2): 351-358.DOI: 10.6048/j.issn.1001-4330.2023.02.012
• 园艺特产·植物保护·微生物·土壤肥料·节水灌溉 • 上一篇 下一篇
收稿日期:
2021-12-11
出版日期:
2023-02-20
发布日期:
2023-03-31
通信作者:
刘慧英(1970-),女,新疆石河子人,教授,研究方向为设施蔬菜栽培生理,(E-mail)hyliuok@aliyun.com
作者简介:
朱普生(1994-),男,江苏连云港人,硕士研究生,研究方向设施蔬菜栽培生理,(E-mail)875920571@qq.com
基金资助:
ZHU Pusheng(), LIU Huiying(), CAO Ze, LIU Kaige, LI Xuezhen
Received:
2021-12-11
Online:
2023-02-20
Published:
2023-03-31
Correspondence author:
LIU Huiying(1970-), female, born in Shihezi, Xinjiang, Professor, majoring in vegetable cultivation physiology of protected horticulture, (E-mail)hyliuok@aliyun.com
Supported by:
摘要:
【目的】 研究NaCl胁迫下外源GSNO(NO供体)对番茄幼苗生长的调节作用。【方法】 以中蔬4号番茄为材料,通过对100 mmol/L NaCl胁迫下番茄幼苗分别喷施0.1 mmol/L GSNO(NO供体)(NG处理)、0.05 mmol/L cPTIO(NO清除剂)(NP处理)、0.1 mmol/L GSNO + 0.05 mmol/L cPTIO (NGP处理),研究外源GSNO对NaCl胁迫下番茄幼苗生长及其盐适应性的调控作用。【结果】 NaCl胁迫下施用GSNO的番茄幼苗生长情况明显好转,叶绿素含量和最大光化学效率(Fv/Fm)提高,Rubisco初始活性和总活性提高,卡尔文循环相关酶Rubisco活化酶(RCA)、磷酸甘油酸激酶(PGK)、磷酸甘油醛脱氢酶(GAPDH)、景天庚酮糖-1,7-二磷酸酶(SBPase)和果糖-1,6-二磷酸酶(FBPase)的活性提高。【结论】 外源GSNO对NaCl胁迫下番茄幼苗生长不良和光合作用降低有缓解作用。
中图分类号:
朱普生, 刘慧英, 曹泽, 刘凯歌, 李雪珍. 外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响[J]. 新疆农业科学, 2023, 60(2): 351-358.
ZHU Pusheng, LIU Huiying, CAO Ze, LIU Kaige, LI Xuezhen. Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 351-358.
处理 Treatment | 株高 Plant height (cm) | 茎粗 Stem diameter (mm) | 地上鲜重 Fresh weight over water (g) | 地下鲜重 Fresh weight under water (g) | 地上干重 Dry weight over water (g) | 地下干重 Dry weight under water (g) | 根冠比 Root/shoot ratio | 壮苗指数 Strong seedling index |
---|---|---|---|---|---|---|---|---|
CK | 29.44±0.69a | 7.77±0.18a | 30.66±2.17a | 4.56±0.22ab | 2.16±0.24a | 0.18±0.01a | 0.08±0.01b | 0.81±0.06a |
NaCl | 20.29±0.2cd | 6.32±0.31c | 12.88±0.67c | 3.94±0.26bc | 0.97±0.04c | 0.12±0.00b | 0.13±0.00a | 0.48±0.02c |
NG | 22.62±0.3b | 6.74±0.08bc | 19.52±0.86b | 5.32±0.4a | 1.5±0.06b | 0.2±0.02a | 0.14±0.01a | 0.74±0.04ab |
NP | 19.53±0.98d | 6.76±0.19bc | 15.64±1.1bc | 3.3±0.18c | 1.22±0.09bc | 0.14±0.01b | 0.11±0.00a | 0.62±0.03b |
NGP | 22.19±0.75bc | 7.28±0.04ab | 18.77±0.9b | 4.61±0.41ab | 1.45±0.08b | 0.18±0.02a | 0.13±0.01a | 0.74±0.04ab |
表1 不同外源GSNO下NaCl胁迫下番茄幼苗生物量变化
Table 1 Effects of exogenous GSNO on tomato seedling biomass under NaCl stress
处理 Treatment | 株高 Plant height (cm) | 茎粗 Stem diameter (mm) | 地上鲜重 Fresh weight over water (g) | 地下鲜重 Fresh weight under water (g) | 地上干重 Dry weight over water (g) | 地下干重 Dry weight under water (g) | 根冠比 Root/shoot ratio | 壮苗指数 Strong seedling index |
---|---|---|---|---|---|---|---|---|
CK | 29.44±0.69a | 7.77±0.18a | 30.66±2.17a | 4.56±0.22ab | 2.16±0.24a | 0.18±0.01a | 0.08±0.01b | 0.81±0.06a |
NaCl | 20.29±0.2cd | 6.32±0.31c | 12.88±0.67c | 3.94±0.26bc | 0.97±0.04c | 0.12±0.00b | 0.13±0.00a | 0.48±0.02c |
NG | 22.62±0.3b | 6.74±0.08bc | 19.52±0.86b | 5.32±0.4a | 1.5±0.06b | 0.2±0.02a | 0.14±0.01a | 0.74±0.04ab |
NP | 19.53±0.98d | 6.76±0.19bc | 15.64±1.1bc | 3.3±0.18c | 1.22±0.09bc | 0.14±0.01b | 0.11±0.00a | 0.62±0.03b |
NGP | 22.19±0.75bc | 7.28±0.04ab | 18.77±0.9b | 4.61±0.41ab | 1.45±0.08b | 0.18±0.02a | 0.13±0.01a | 0.74±0.04ab |
图1 不同外源GSNO下NaCl胁迫下番茄幼苗气孔数目与根系形态变化 注:A:对气孔数目的影响;B:对根系总长度的影响;C:对根系总面积的影响;D:对根系总体积的影响;小写字母表示处理间在5%水平的差异显著性,下同
Fig.1 Effects of exogenous GSNO on stomatal number and root morphology of Tomato Seedlings under NaCl stress Note: A:the effect on stomatal number; B:the effect on total root length; C:the effect on total root area; D:the effect on total root volume; different small letters above figures indicates significant difference at 5% leve, the same as below
图2 不同外源GSNO下NaCl胁迫下番茄幼苗叶绿素含量变化 注:A:对叶绿素a含量的影响;B:对叶绿素b含量的影响;C:对总叶绿素含量的影响
Fig.2 Effect of exogenous GSNO on chlorophyll content of Tomato Seedlings under NaCl stress Note:A:the effect on chlorophyll a content, B:the effect on chlorophyll b content, C:the effect on the content of total chlorophyll
图3 不同外源GSNO下NaCl胁迫下番茄幼苗叶绿素荧光参数变化 注:A:对最大光化学效率(Fv/Fm)的影响;B:对实际光化学效率(YII)的影响;C:对化学淬灭系数(qP)的影响;D:对光合电子传递速率(ETR)的影响
Fig.3 Effects of exogenous GSNO on chlorophyll fluorescence parameters of Tomato Seedlings under NaCl stress Note: A:the effect on maximum photochemical efficiency (Fv / Fm); B:the effect on actual photochemical efficiency (YII); C:the effect on chemical quenching coefficient (qP); D:the effect on photosynthetic electron transfer rate (ETR)
指标 Index | 处理 Treatment | ||||
---|---|---|---|---|---|
CK | N | NG | NP | NGP | |
初始活性Rubisco(U/g) | 9.85±0.27a | 6.61±0.34c | 8.67±0.27b | 5.40±0.50d | 8.20±0.42b |
总活性Rubisco(U/g) | 30.82±0.72a | 12.59±0.28d | 24.34±1.27b | 15.40±0.66c | 17.10±0.82c |
活化酶Rubisco(RCA)活性(mU/g) | 1 827.33±87.05a | 1 221.15±83.85d | 1 672.63±22.10b | 1 114.68±36.29e | 1 356.19±7.71c |
磷酸甘油酸激酶(PGK)活性(U/g) | 1.79±0.02a | 1.40±0.04c | 1.55±0.06b | 1.15±0.05d | 1.44±0.02c |
磷酸甘油醛脱氢酶(GAPDH)活性(U/g) | 0.47±0.01a | 0.24±0.01d | 0.42±0.01b | 0.21±0.01e | 0.40±0.01c |
景天庚酮糖-1,7-二磷酸酶(SBPase) 活性(U/g) | 1.31±0.10a | 0.43±0.01d | 0.67±0.05c | 0.48±0.02d | 0.78±0.01b |
果糖-1,6-二磷酸酶(FBPase) 活性(mU/g) | 937.38±10.82a | 837.33±3.11b | 936.85±7.80a | 794.68±10.80c | 857.13±29.93b |
果糖-1,6-二磷酸醛缩酶(FBA) 活性(mU/g) | 1.38±0.03a | 1.27±0.04b | 1.22±0.05b | 1.01±0.02c | 1.28±0.03b |
表2 不同外源GSNO下NaCl胁迫下番茄幼苗卡尔文循环相关酶活性变化
Table 2 Effects of exogenous GSNO on activities of Calvin cycle related enzymes in tomato seedlings under NaCl stress
指标 Index | 处理 Treatment | ||||
---|---|---|---|---|---|
CK | N | NG | NP | NGP | |
初始活性Rubisco(U/g) | 9.85±0.27a | 6.61±0.34c | 8.67±0.27b | 5.40±0.50d | 8.20±0.42b |
总活性Rubisco(U/g) | 30.82±0.72a | 12.59±0.28d | 24.34±1.27b | 15.40±0.66c | 17.10±0.82c |
活化酶Rubisco(RCA)活性(mU/g) | 1 827.33±87.05a | 1 221.15±83.85d | 1 672.63±22.10b | 1 114.68±36.29e | 1 356.19±7.71c |
磷酸甘油酸激酶(PGK)活性(U/g) | 1.79±0.02a | 1.40±0.04c | 1.55±0.06b | 1.15±0.05d | 1.44±0.02c |
磷酸甘油醛脱氢酶(GAPDH)活性(U/g) | 0.47±0.01a | 0.24±0.01d | 0.42±0.01b | 0.21±0.01e | 0.40±0.01c |
景天庚酮糖-1,7-二磷酸酶(SBPase) 活性(U/g) | 1.31±0.10a | 0.43±0.01d | 0.67±0.05c | 0.48±0.02d | 0.78±0.01b |
果糖-1,6-二磷酸酶(FBPase) 活性(mU/g) | 937.38±10.82a | 837.33±3.11b | 936.85±7.80a | 794.68±10.80c | 857.13±29.93b |
果糖-1,6-二磷酸醛缩酶(FBA) 活性(mU/g) | 1.38±0.03a | 1.27±0.04b | 1.22±0.05b | 1.01±0.02c | 1.28±0.03b |
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