NaCl胁迫对糜子种子萌发的影响

doi:10.6048/j.issn.1001-4330.2023.07.008

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (7): 1623-1629.DOI: 10.6048/j.issn.1001-4330.2023.07.008

• 作物遗传育种·种质资源·分子遗传学·土壤肥料 • 上一篇下一篇

NaCl胁迫对糜子种子萌发的影响

肖菁¹(), 刘宁¹^,², 许明海³, 张金波¹, 马艳明¹, 王莉¹(), 徐麟¹()

1.新疆农业科学院农作物品种资源研究所,乌鲁木齐 830091
2.农业部植物新品种测试(乌鲁木齐)分中心,乌鲁木齐 830091
3.疏勒县农业技术推广中心,新疆疏勒 844200

收稿日期:2022-10-11 出版日期:2023-07-20 发布日期:2023-07-11
通信作者: 徐麟(1968-),男,湖北黄梅人,研究员,研究方向为农作物种质资源创新,(E-mail)hnsyxulin@163.com；
王莉(1976-),女,山东胶南人,副研究员,硕士,研究方向为农作物种质资源收集保存与评价利用,(E-mail)wli06@126.com
作者简介:肖菁(1979-),女,河北人,助理研究员,研究方向为农作物种质资源收集保存与评价利用,(E-mail)49769826@qq.com
基金资助:
农业农村部第三次全国农作物种质资源普查与收集行动项目(19210818);新疆农业科学院自主培育项目(nkyzztd-004)

Effect of NaCl stress on seed germination of Panicum miliaceum L.

XIAO Jing¹(), LIU Ning¹^,², XU Minghai³, ZHANG Jinbo¹, MA Yanming¹, WANG Li¹(), XU Lin¹()

1. Institute of Crop Germplasm Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091, China
2. Urumqi Station for DUS Testing Center of New Plant Varieties,Ministry of Agriculture and Rural Affairs,Urumqi 830091, China
3. Agricultural Technology Extension Center of Shule County, Shule Xinjiang 844200, China

Received:2022-10-11 Online:2023-07-20 Published:2023-07-11
Correspondence author: XU Lin (1968-),male, Huang Mei, Hubei province, undergraduate, researcher, research direction is innovative research of crop germplasm resources, (E-mail) hnsyxulin@163.com；
WANG Li (1976-), female, from Jiaonan, Shandong province, associate researcher, master, research direction for the collection, preservation, evaluation and utilization of crop germplasm resources, (E-mail)wli06@126.com
Supported by:
Third National Survey and Collection Project of Ministry of Agriculture and Rural Affairs (19221808);Independent Cultivation Project of Xinjiang Academy of Agricultural Sciences(nkyzztd-004)

摘要/Abstract

摘要：

【目的】研究NaCl胁迫对糜子种子萌发的影响,分析比较内蒙古糜子186和新疆黄糜MZ04的耐盐性。【方法】于种子萌芽期处理参试材料,统计发芽势、发芽率、胚根长度、胚茎长度,计算发芽指数,活力指数。【结果】NaCl胁迫过程中,参试材料种子发芽势、发芽率、发芽指数、活力指数、胚根、胚茎长度差异显著,随着NaCl溶液浓度的增加,各个指标均降低,在200、250 mmol/L处理下MZ04的发芽率、发芽势、发芽指数、活力指数明显高于内蒙古糜子186;且在不同浓度NaCl处理下MZ04的胚根、胚茎长度均高于内蒙古糜子186;NaCl胁迫过程中,糜子种子萌发期各指标与NaCl浓度呈负相关,MZ04各个指标的耐盐半抑制浓度均高于内蒙古糜子186;MZ04的发芽率耐盐半抑制浓度为107.39 mmol/L,较内蒙古糜子186高出4.18 mmol/L,活力指数耐盐半抑制浓度为45.81 mmol/L,较内蒙古糜子186高出5.2 mmol/L。【结论】糜子在盐胁迫过程中,萌发相关指标主要是由盐浓度及品种的耐盐特性决定,新疆黄糜MZ04种子萌发期的耐盐性高于内蒙古糜子186。

关键词: NaCl胁迫; 糜子; 种子萌发

Abstract:

【Objective】 To study the effect of NaCl stress on Seed Germination of chime by analyzing and comparing the salt tolerance of Inner Mongolia chyme 186 and Xinjiang chyme MZ04.【Methods】 In this study, the materials were treated by seed germination test, and the germination potential, germination rate, radicle root length, radicle stem length, germination index and vigor index were calculated.In the process, there were significant differences in germination power, germination rate, germination index, vigor index, radicle and radicle length among the tested materials.【Results】 With the increase of NaCl concentration, each index decreased at 200 and 250 mmol / L.The germination rate, germination potential, germination index and vigor index of Xinjiang chyme MZ04 under mmol / L treatment were significantly higher than those of Inner Mongolia chyme 186 in mainland China, and the radicle and radicle length of Xinjiang chyme MZ04 under different concentrations of NaCl treatment were higher than those of Inner Mongolia chyme 186 in mainland China.Through statistical regression analysis, it was found that during the process of NaCl stress, the concentration of NaCl was negatively correlated, and the concentration of salt tolerance semi inhibition of Xinjiang chyme MZ04 was higher than that of Inner Mongolia chyme 186.The germination rate of Inner Mongolia chyme 186 and Xinjiang chyme MZ04 was 107.39 mmol / L, 4.18 mmol / L higher than that of Inner Mongolia chyme 186, the activity index was 45.81 mmol / L, 5.2 mmol / L higher than that of Inner Mongolia chyme 186.【Conclusion】 In the process of salt stress, the related indexes of germination are mainly determined by salt concentration and salt tolerance of varieties, and the salt tolerance of Xinjiang chyme MZ04 seed in germination period is higher than that of chyme 186.

Key words: NaCl stress; Panicum miliaceum L.; seed germination

中图分类号:

S516

肖菁, 刘宁, 许明海, 张金波, 马艳明, 王莉, 徐麟. NaCl胁迫对糜子种子萌发的影响[J]. 新疆农业科学, 2023, 60(7): 1623-1629.

XIAO Jing, LIU Ning, XU Minghai, ZHANG Jinbo, MA Yanming, WANG Li, XU Lin. Effect of NaCl stress on seed germination of Panicum miliaceum L.[J]. Xinjiang Agricultural Sciences, 2023, 60(7): 1623-1629.

图/表 8

参考文献 21

[1]	袁雨豪, 杨清华, 党科, 等. 糜子资源耐盐性评价与盐胁迫生理响应[J]. 中国农业科学, 2019, 52(22):4066-4090. DOI
	YUAN Yuhao, YANG Qinghua, DANG Ke, et al. Evaluation of salt tolerance and physiological response to salt stress in Millet resources[J]. Scientia Agricultura Sinica, 2019, 52(22):4066-4090.
[2]	刘星宏, 张青青, 张鹏, 等. 北疆盐碱地空间分布特征分析[J/OL]. 中国农业科技导报:1-8.
	LIU Xinghong, ZHANG Qingqing, ZHANG Peng, et al. Analysis of spatial distribution characteristics of saline alkali land in Northern Xinjiang [J/OL].China Agricultural Science and technology Herald:1-8.
[3]	田长彦, 买文选, 赵振勇. 新疆干旱区盐碱地生态治理关键技术研究[J]. 生态学报, 2016, 36(22):7064-7068.
	TIAN Changyan, MAI Wenxuan, ZHAO Zhenyong. Study on Key Technologies of ecological management of saline alkali land in arid area of Xinjiang[J]. Journal of Ecology, 2016, 36(22):7064-7068.
[4]	张洪鹏, 陈德龙, 马强, 等. 不同糜子品种芽期耐盐性比较[J]. 安徽农业科学, 2016, 44(23):11-13.
	ZHANG Hongpeng, CHEN Delong, MA Qiang, et al. Comparison of salt tolerance of different millet varieties at bud stage[J]. Journal of Anhui Agricultural Sciences, 2016, 44(23):11-13.
[5]	刘敏轩, 张宗文, 吴斌, 等. 黍稷种质资源芽、苗期耐中性混合盐胁迫评价与耐盐生理机制研究[J]. 中国农业科学, 2012, 45(18):3733-3743. DOI
	LIU Minxuan, ZHANG Zongwen, WU Bin, et al. Evaluation and physiological mechanism of tolerance to neutral mixed salt stress in bud and seedling of Millet Germplasm Resources[J]. Scientia Agricultura Sinica, 2012, 45(18):3733-3743. DOI
[6]	李占成, 张丽丽, 李玮, 等. 盐胁迫对糜子种子发芽的影响[J]. 作物杂志, 2011,(6):122-123.
	LI Zhancheng, ZHANG Lili, LI Wei, et al. Effect of salt stress on Seed Germination of millet[J]. Crop Journal, 2011,(6):122-123.
[7]	王纶, 王星玉, 温琪汾, 等. 中国黍稷种质资源耐盐性鉴定[J]. 植物遗传资源学报, 2007(4):426-429.
	WANG Lun, WANG Xingyu, WEN Qifen, et al. Identification of salt tolerance of Millet Germplasm Resources in China[J]. Journal of Plant Genetic Resources, 2007(4):426-429.
[8]	王治江, 刘自刚, 孙万仓, 等. NaCl和Na₂SO₄胁迫对白菜型冬油菜种子萌发的影响及其耐盐性分析[J]. 干旱地区农业研究, 2016, 34(6):243-252.
	WANG Zhijiang, LIU Zigang, SUN Wancang, et al. NaCl and Na₂SO₄.Effect of stress on Seed Germination of Brassica campestris and analysis of salt tolerance[J]. Agricultural Research in Arid Areas, 2016, 34 (6):243-252.
[9]	王康君, 樊继伟, 陈凤, 等. 植物对盐胁迫的响应及耐盐调控的研究进展[J]. 江西农业学报, 2018, 30(12):31-40.
	WANG Kangjun, FAN Jiwei, CHEN Feng, et al. Response of plants to salt stress and research progress of salt tolerance regulation[J]. Acta Agriculturae Jiangxi, 2018, 30(12):31-40
[10]	王悦霖, 叶协锋, 宋佳倩, 等. 基于人工气候培养箱的34个烟草品种萌发期耐盐性评价[J/OL]. 烟草科技:1-17.
	WANG Yuelin, YE Xiefeng, SONG Jiaqian, et al. Salt tolerance evaluation of 34 tobacco varieties at germination stage based on artificial climate incubator [J/OL].Tobacco Science and Technology:1-17.
[11]	马跃峰, 唐敬芹, 王建林. Na₂CO₃胁迫对青稞种子萌发及幼苗生长的影响[J]. 高原农业, 2020, 4(2):123-130.
	MA Yuefeng, TANG Jingqin, WANG Jianlin. Na₂CO₃ Effects of stress on Seed Germination and seedling growth of highland barley[J]. Plateau Agriculture, 2020, 4(2):123-130.
[12]	高英波, 张慧, 薛艳芳, 等. 不同夏玉米品种耐盐性综合评价与耐盐品种筛选[J]. 玉米科学, 2020, 28(2):33-40.
	GAO Yingbo, ZHANG Hui, XUE Yanfang, et al. Comprehensive evaluation of salt tolerance of different summer maize varieties and selection of salt tolerant varieties[J]. Journal of Maize Sciences, 2020, 28 (2):33-40.
[13]	李雪, 沙栢平, 高雪芹, 等. 不同紫花苜蓿种质材料萌发期耐盐性鉴定与综合评价[J]. 草地学报, 2020, 28(2):437-445. DOI
	LI Xue, SHA Peiping, GAO Xueqin, et al. Identification and comprehensive evaluation of salt tolerance of different alfalfa germplasm materials at germination stage[J]. Acta Agrestia Sinica, 2020, 28 (2):437-445. DOI
[14]	库尼都孜阿依·吐尔汗, 任毅, 颜安, 等. 新疆冬小麦品种萌发期耐盐性综合评价及耐盐种质的筛选[J]. 新疆农业科学, 2020, 57(1):20-31. DOI
	Kuniduayi Turhan, REN Yi, YAN An, et al. Comprehensive evaluation of salt tolerance and selection of salt tolerant germplasm at germination stage of Xinjiang Winter Wheat Varieties[J]. Xinjiang Agricultural Sciences, 2020, 57(1):20-31.
[15]	荆瑞勇, 王丽艳, 郑桂萍, 等. 水稻萌发期和幼苗期耐盐性鉴定指标筛选及综合评价[J]. 黑龙江八一农垦大学学报, 2019, 31(6):1-6,19.
	JING Ruiyong, WANG Liyan, ZHENG Guiping, et al. Selection and comprehensive evaluation of salt tolerance identification indexes in rice germination and seedling stage[J]. Journal of Heilongjiang Bayi Agricultural Reclamation University, 2019, 31(6):1-6,19.
[16]	李源, 朱倩倩, 许咏梅. NaCl胁迫对几种典型耐盐经济作物种子萌发的影响[J]. 中国土壤与肥料, 2020,(2):226-231.
	LI Yuan, ZHU Qianqian, XU Yongmei. Effect of NaCl stress on Seed Germination of several typical salt tolerant cash crops[J]. Soils and Fertilizers Sciences in China, 2020,(2):226-231.
[17]	李莉, 张科, 何明才, 等. 不同盐浓度对四个品种番茄种子萌发和幼苗芽长的影响[J]. 北方园艺, 2019,(24):1-6.
	LI Li, ZHANG Ke, HE Mingcai, et al. Effects of different salt concentrations on Seed Germination and seedling bud growth of four tomato varieties[J]. Northern Horticulture, 2019,(24):1-6.
[18]	周述波, 贺立红, 林伟, 等,黎明. 盐胁迫对海南山兰稻种子萌发及幼苗期生理特性的影响[J/OL]. 分子植物育种:1-14.
	ZHOU Shubo, HE Lihong, LIN Wei, et al., liming. Effects of salt stress on Seed Germination and Seedling Physiological Characteristics of hainanshan blue rice[J/OL].Molecular Plant Breeding:1-14.
[19]	权有娟, 袁飞敏, 李想, 等. NaCl胁迫对藜麦幼苗生长及生理特性的影响[J/OL]. 广西植物:1-8.
	QUAN Youjuan, YUAN Feimin, LI Xiang, et al. Effects of NaCl stress on the growth and physiological characteristics of quinoa seedlings [J/OL]. Guihaia:1-8.
[20]	张贝贝, 樊佳茹, 王景荣, 等. 模拟盐渍化对甜瓜种子萌发和幼苗生理特性的影响[J/OL]. 热带作物学报:1-8.
	ZHANG Beibei, FAN Jiaru, WANG Jingrong, et al. Effects of simulated salinization on Seed Germination and Seedling Physiological Characteristics of muskmelon [J/OL]. Chinese Journal of Tropical Crops:1-8.
[21]	王亚, 杨俊芳, 王宙, 等. 蓖麻种子萌发与幼苗生长对盐胁迫的生理响应[J]. 山西农业科学, 2019, 47(10):1705-1708.
	WANG Ya, YANG Junfang, WANG Zhou, et al. Physiological response of Castor Seed Germination and seedling growth to salt stress[J]. Shanxi Agricultural Sciences, 2019, 47(10):1705-1708.

材料 Material Science	出苗期 Emergence stage	抽穗期 Heading stage	成熟期 Mature period	生育期 Growth period (d)	株高 Plant height (cm)	穗长 Spike length (cm)	叶长 Leaf length (cm)	穗重 Ear weight (g)	穗粒重 Ear and grain weight (g)
新疆黄糜MZ04 Xinjiang chyme MZ04	4月18日	6月9日	7月15日	94	101.7	43.7	30.7	23.9	17.4
内蒙古糜子186 Inner Mongolia chyme 186	4月21日	6月17日	7月31日	101	129.2	44.8	37.3	27.9	17.4

材料 Material Science	出苗期 Emergence stage	抽穗期 Heading stage	成熟期 Mature period	生育期 Growth period (d)	株高 Plant height (cm)	穗长 Spike length (cm)	叶长 Leaf length (cm)	穗重 Ear weight (g)	穗粒重 Ear and grain weight (g)
新疆黄糜MZ04 Xinjiang chyme MZ04	4月18日	6月9日	7月15日	94	101.7	43.7	30.7	23.9	17.4
内蒙古糜子186 Inner Mongolia chyme 186	4月21日	6月17日	7月31日	101	129.2	44.8	37.3	27.9	17.4

材料 Material Science	指标 Index	回归方程 Regression equation	R²	耐盐半抑制浓度 Salt tolerance semi inhibition concentration(mmol/L)
新疆黄糜MZ04 Xinjiang chyme MZ04	发芽势	y=-197.496x+232.635	0.897	93.32
	发芽率	y=-271.759x+341.502	0.603	107.39
	发芽指数	y=-278.345x+353.24	0.693	126.73
	活力指数	y=-614.342x+281.657	0.986	45.81
	胚根长度	y=-5.714x+256.255	0.949	40.57
	胚茎长度	y=-6.579x+256.289	0.974	35.97
内蒙古糜子186 Inner Mongolia chyme 186	发芽势	y=-207.582x+213.222	0.927	81.84
	发芽率	y=-188.577x+241.289	0.802	103.21
	发芽指数	y=-193.957x+246.223	0.797	126.71
	活力指数	y=-564.304x+229.369	0.971	40.61
	胚根长度	y=-3.777x+199.233	0.848	34.33
	胚茎长度	y=-6.748x+222.242	0.967	30.35

材料 Material Science	指标 Index	回归方程 Regression equation	R²	耐盐半抑制浓度 Salt tolerance semi inhibition concentration(mmol/L)
新疆黄糜MZ04 Xinjiang chyme MZ04	发芽势	y=-197.496x+232.635	0.897	93.32
	发芽率	y=-271.759x+341.502	0.603	107.39
	发芽指数	y=-278.345x+353.24	0.693	126.73
	活力指数	y=-614.342x+281.657	0.986	45.81
	胚根长度	y=-5.714x+256.255	0.949	40.57
	胚茎长度	y=-6.579x+256.289	0.974	35.97
内蒙古糜子186 Inner Mongolia chyme 186	发芽势	y=-207.582x+213.222	0.927	81.84
	发芽率	y=-188.577x+241.289	0.802	103.21
	发芽指数	y=-193.957x+246.223	0.797	126.71
	活力指数	y=-564.304x+229.369	0.971	40.61
	胚根长度	y=-3.777x+199.233	0.848	34.33
	胚茎长度	y=-6.748x+222.242	0.967	30.35

NaCl胁迫对糜子种子萌发的影响

Effect of NaCl stress on seed germination of Panicum miliaceum L.

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[14]	何晨晨, 刘俐君, 鲁晓燕. 新疆野苹果幼苗叶片响应NaCl胁迫的miRNAs及靶基因筛选[J]. 新疆农业科学, 2021, 58(4): 589-598.
[15]	麻冬, 艾比布拉·伊马木, 哈里木拉提·努热合买提. 木地肤种子包衣材料的筛选[J]. 新疆农业科学, 2021, 58(2): 352-360.