外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响

doi:10.6048/j.issn.1001-4330.2023.02.012

新疆农业科学 ›› 2023, Vol. 60 ›› Issue (2): 351-358.DOI: 10.6048/j.issn.1001-4330.2023.02.012

• 园艺特产·植物保护·微生物·土壤肥料·节水灌溉 • 上一篇下一篇

外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响

朱普生(), 刘慧英(), 曹泽, 刘凯歌, 李雪珍

石河子大学农学院园艺系/特色果蔬栽培生理与种质资源利用新疆生产建设兵团重点实验室,新疆石河子 832000

收稿日期:2021-12-11 出版日期:2023-02-20 发布日期:2023-03-31
通信作者: 刘慧英(1970-),女,新疆石河子人,教授,研究方向为设施蔬菜栽培生理,(E-mail)hyliuok@aliyun.com
作者简介:朱普生(1994-),男,江苏连云港人,硕士研究生,研究方向设施蔬菜栽培生理,(E-mail)875920571@qq.com
基金资助:
国家自然科学基金“蛋白质谷胱甘肽化修饰调控番茄光合碳同化及耐盐性的作用机制”(31860550)

Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress

ZHU Pusheng(), LIU Huiying(), CAO Ze, LIU Kaige, LI Xuezhen

Key Laboratory of Cultivation Physiology and Germplasm Resources Utilization of Characteristic Fruits and Vegetables of Xinjiang Production and Construction Corps/College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China

Received:2021-12-11 Online:2023-02-20 Published:2023-03-31
Correspondence author: LIU Huiying(1970-), female, born in Shihezi, Xinjiang, Professor, majoring in vegetable cultivation physiology of protected horticulture, (E-mail)hyliuok@aliyun.com
Supported by:
National Natural Science Foundation of China “The Mechanism of Glutathione Modification of Protein Regulating Photosynthetic Carbon Assimilation and Salt Tolerance of Tomato”(31860550)

摘要/Abstract

摘要：

【目的】 研究NaCl胁迫下外源GSNO(NO供体)对番茄幼苗生长的调节作用。【方法】 以中蔬4号番茄为材料,通过对100 mmol/L NaCl胁迫下番茄幼苗分别喷施0.1 mmol/L GSNO(NO供体)(NG处理)、0.05 mmol/L cPTIO(NO清除剂)(NP处理)、0.1 mmol/L GSNO + 0.05 mmol/L cPTIO (NGP处理),研究外源GSNO对NaCl胁迫下番茄幼苗生长及其盐适应性的调控作用。【结果】 NaCl胁迫下施用GSNO的番茄幼苗生长情况明显好转,叶绿素含量和最大光化学效率(Fv/Fm)提高,Rubisco初始活性和总活性提高,卡尔文循环相关酶Rubisco活化酶(RCA)、磷酸甘油酸激酶(PGK)、磷酸甘油醛脱氢酶(GAPDH)、景天庚酮糖-1,7-二磷酸酶(SBPase)和果糖-1,6-二磷酸酶(FBPase)的活性提高。【结论】 外源GSNO对NaCl胁迫下番茄幼苗生长不良和光合作用降低有缓解作用。

关键词: 番茄; GSNO; NaCl胁迫; 光合作用

Abstract:

【Objective】 To study the effect of exogenous GSNO (exogenous NO donor) on alleviating NaCl stress of tomato. 【Methods】 In this study, tomato cultivar Zhongshu 4 was used as experimental material. Tomato Seedlings under NaCl stress (100 mmol/L) were treated with NG:0.5 mmol/L GSNO (S-nitrosoglutathione, NO donor), NP: 1 mmol/L cPTIO (NO scavenger), NGP: 0.5 mmol/L GSNO compound 1 mmol/L cPTIO; The effects of exogenous GSNO on the growth and salt tolerance of tomato seedlings under NaCl stress were studied. 【Results】 After applying GSNO, the growth of tomato seedlings was significantly improved under NaCl stress, the chlorophyll content increased, the chlorophyll fluorescence parameters returned to normal, and the Calvin cycle enzyme activity returned to normal level. 【Conclusion】 This study shows that exogenous GSNO can alleviate the adverse growth and photosynthetic effect of tomato seedlings under NaCl stress.

Key words: tomato; GSNO; NaCl stress; photosynthesis

中图分类号:

S641.2

朱普生, 刘慧英, 曹泽, 刘凯歌, 李雪珍. 外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响[J]. 新疆农业科学, 2023, 60(2): 351-358.

ZHU Pusheng, LIU Huiying, CAO Ze, LIU Kaige, LI Xuezhen. Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2023, 60(2): 351-358.

图/表 5

表1 不同外源GSNO下NaCl胁迫下番茄幼苗生物量变化

Table 1 Effects of exogenous GSNO on tomato seedling biomass under NaCl stress

处理 Treatment	株高 Plant height (cm)	茎粗 Stem diameter (mm)	地上鲜重 Fresh weight over water (g)	地下鲜重 Fresh weight under water (g)	地上干重 Dry weight over water (g)	地下干重 Dry weight under water (g)	根冠比 Root/shoot ratio	壮苗指数 Strong seedling index
CK	29.44±0.69^a	7.77±0.18^a	30.66±2.17^a	4.56±0.22^ab	2.16±0.24^a	0.18±0.01^a	0.08±0.01^b	0.81±0.06^a
NaCl	20.29±0.2^cd	6.32±0.31^c	12.88±0.67^c	3.94±0.26^bc	0.97±0.04^c	0.12±0.00^b	0.13±0.00^a	0.48±0.02^c
NG	22.62±0.3^b	6.74±0.08^bc	19.52±0.86^b	5.32±0.4^a	1.5±0.06^b	0.2±0.02^a	0.14±0.01^a	0.74±0.04^ab
NP	19.53±0.98^d	6.76±0.19^bc	15.64±1.1^bc	3.3±0.18^c	1.22±0.09^bc	0.14±0.01^b	0.11±0.00^a	0.62±0.03^b
NGP	22.19±0.75^bc	7.28±0.04^ab	18.77±0.9^b	4.61±0.41^ab	1.45±0.08^b	0.18±0.02^a	0.13±0.01^a	0.74±0.04^ab

图1 不同外源GSNO下NaCl胁迫下番茄幼苗气孔数目与根系形态变化注:A:对气孔数目的影响;B:对根系总长度的影响;C:对根系总面积的影响;D:对根系总体积的影响;小写字母表示处理间在5%水平的差异显著性,下同

Fig.1 Effects of exogenous GSNO on stomatal number and root morphology of Tomato Seedlings under NaCl stress Note: A:the effect on stomatal number; B:the effect on total root length; C:the effect on total root area; D:the effect on total root volume; different small letters above figures indicates significant difference at 5% leve, the same as below

图2 不同外源GSNO下NaCl胁迫下番茄幼苗叶绿素含量变化注:A:对叶绿素a含量的影响;B:对叶绿素b含量的影响;C:对总叶绿素含量的影响

Fig.2 Effect of exogenous GSNO on chlorophyll content of Tomato Seedlings under NaCl stress Note:A:the effect on chlorophyll a content, B:the effect on chlorophyll b content, C:the effect on the content of total chlorophyll

图3 不同外源GSNO下NaCl胁迫下番茄幼苗叶绿素荧光参数变化注:A:对最大光化学效率(Fv/Fm)的影响;B:对实际光化学效率(YII)的影响;C:对化学淬灭系数(qP)的影响;D:对光合电子传递速率(ETR)的影响

Fig.3 Effects of exogenous GSNO on chlorophyll fluorescence parameters of Tomato Seedlings under NaCl stress Note: A:the effect on maximum photochemical efficiency (Fv / Fm); B:the effect on actual photochemical efficiency (YII); C:the effect on chemical quenching coefficient (qP); D:the effect on photosynthetic electron transfer rate (ETR)

表2 不同外源GSNO下NaCl胁迫下番茄幼苗卡尔文循环相关酶活性变化

Table 2 Effects of exogenous GSNO on activities of Calvin cycle related enzymes in tomato seedlings under NaCl stress

指标 Index	处理 Treatment
指标 Index	CK	N	NG	NP	NGP
初始活性Rubisco(U/g)	9.85±0.27^a	6.61±0.34^c	8.67±0.27^b	5.40±0.50^d	8.20±0.42^b
总活性Rubisco(U/g)	30.82±0.72^a	12.59±0.28^d	24.34±1.27^b	15.40±0.66^c	17.10±0.82^c
活化酶Rubisco(RCA)活性(mU/g)	1 827.33±87.05^a	1 221.15±83.85^d	1 672.63±22.10^b	1 114.68±36.29^e	1 356.19±7.71^c
磷酸甘油酸激酶(PGK)活性(U/g)	1.79±0.02^a	1.40±0.04^c	1.55±0.06^b	1.15±0.05^d	1.44±0.02^c
磷酸甘油醛脱氢酶(GAPDH)活性(U/g)	0.47±0.01^a	0.24±0.01^d	0.42±0.01^b	0.21±0.01^e	0.40±0.01^c
景天庚酮糖-1,7-二磷酸酶(SBPase) 活性(U/g)	1.31±0.10^a	0.43±0.01^d	0.67±0.05^c	0.48±0.02^d	0.78±0.01^b
果糖-1,6-二磷酸酶(FBPase) 活性(mU/g)	937.38±10.82^a	837.33±3.11^b	936.85±7.80^a	794.68±10.80^c	857.13±29.93^b
果糖-1,6-二磷酸醛缩酶(FBA) 活性(mU/g)	1.38±0.03^a	1.27±0.04^b	1.22±0.05^b	1.01±0.02^c	1.28±0.03^b

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外源GSNO对NaCl胁迫下番茄幼苗生长及光合特性的影响

Effects of Exogenous GSNO on Growth and Photosynthetic Characteristics of Tomato Seedlings under NaCl Stress

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