新疆农业科学 ›› 2021, Vol. 58 ›› Issue (4): 589-598.DOI: 10.6048/j.issn.1001-4330.2021.04.001
• 作物遗传育种·种质资源·分子遗传学·耕作栽培 • 下一篇
何晨晨, 刘俐君, 鲁晓燕
收稿日期:
2020-03-05
出版日期:
2021-04-20
发布日期:
2021-04-25
通信作者:
鲁晓燕(1970-),女,新疆人,教授,博士,博士生导师,研究方向为园艺果树学,(E-mail)lxyshz@126.com
作者简介:
何晨晨(1995-),女,河南人,硕士研究生,研究方向为园艺果树学,(E-mail)1660038110@qq.com
基金资助:
HE Chenchen, LIU Lijun, LU Xiaoyan
Received:
2020-03-05
Online:
2021-04-20
Published:
2021-04-25
Correspondence author:
LU Xiaoyan(1970-), female, professor, PhD, Research Direction: Horticulture and pomology, (E-mail)lxyshz@126.com
Supported by:
摘要: 【目的】 筛选新疆野苹果响应NaCl胁迫相关差异miRNA及靶基因。【方法】 以新疆野苹果组培苗为材料,对150 mmol/L NaCl处理6和48 h时的新疆野苹果幼苗叶片进行small RNA测序。【结果】 NaCl处理6 h时3个miRNA差异表达,其中2个上调表达,1个下调表达,miRNA对应的靶基因数目为64个;NaCl处理48 h时13个miRNA差异表达,其中4个上调表达,9个下调表达,miRNA对应的靶基因数目为108个。对差异靶基因进行GO和KEGG分类注释,NaCl处理6 h时,GO主要富集包括:肌醇磷酸2激酶活性、寡糖基转移酶活性、蛋白丝氨酸/苏氨酸磷酸酶抑制剂活性、木聚糖生物合成过程等;NaCl处理48 h时,GO主要富集包括:氧氧化还原酶活性、DNA结合、质外体、次生代谢过程等。KEGG共同富集在N-glycan生物合成通路。【结论】 mdm-miR168b、mdm-miR159c、mdm-miR827、mdm-miR390f、mdm-miR171i、mdm-miR399j基因在NaCl处理6和48 h时表达量存在差异,其中mdm-miR390f及其靶基因HF10976、mdm-miR171i及其靶基因HF33844、mdm-miR399j及其靶基因HF11095表达量呈负相关,3个miRNA参与了新疆野苹果对NaCl胁迫的响应过程。
中图分类号:
何晨晨, 刘俐君, 鲁晓燕. 新疆野苹果幼苗叶片响应NaCl胁迫的miRNAs及靶基因筛选[J]. 新疆农业科学, 2021, 58(4): 589-598.
HE Chenchen, LIU Lijun, LU Xiaoyan. Selection of miRNAs and Target Genes in Leaves of Malus sieversii under NaCl Stress[J]. Xinjiang Agricultural Sciences, 2021, 58(4): 589-598.
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