外源CaCl2缓解NaCl胁迫下酸枣幼苗叶和根转录组测序分析

doi:10.6048/j.issn.1001-4330.2019.06.008

摘要/Abstract

摘要： 【目的】研究CaCl₂缓解酸枣盐胁迫的分子机制。通过转录组测序分析,为研究CaCl₂缓解酸枣盐害分子机制提供参考。【方法】研究以水培酸枣幼苗为材料,150 mmol/L NaCl和150 mmol/L NaCl + 10 mmol/L CaCl₂分别处理6 h,利用高通量测序技术对酸枣幼苗叶片和根系进行了转录组测序与组装,对获得的所有Unigene在GO、KEGG数据库中进行功能注释。【结果】与对照组相比,在外源CaCl₂处理下酸枣幼苗叶片上调和下调的差异表达基因分别7 365个和1 247个,根系分别有762个和4 861个。【结论】GO数据库比对,酸枣幼苗叶片富集了43个GO条件,根系富集了42个GO条件,叶片和根系均是催化活性被富集到差异基因最多;KEGG数据库比对,差异基因主要涉及植物病原互作、MAPK信号通路-植物、植物激素信号转导、苯丙醇生物合成、戊糖和葡萄糖醛酸相互转化等。

关键词: 酸枣; CaCl₂; NaCl胁迫; 转录组

Abstract: 【Objective】 To study the molecular mechanism of CaCl₂ alleviating salt stress in sour jujube seedlings.This study provides a reference for the study of the molecular mechanism of CaCl₂ to alleviate the salt damage of sour jujube (Zizyphus jujuba Mill.) through transcriptome sequencing analysis.【Method】In this study, the seedlings of sour jujube were treated with 150 mmol/L NaCl and 150 mmol /L NaCl + 10 mmol /L CaCl₂ for 6 hours respectively. The transcriptome sequencing and assembly of leaves and roots of sour jujube seedlings were carried out by high-throughput sequencing technique,and all Unigenes obtained were annotated in GO and KEGG databases. 【Result】The results showed that compared with the control group, under exogenous CaCl₂ treatment, 7,365 and 1,247 differential expressed genes were up-regulated and down-regulated in the leaves of sour jujube seedlings, 762 and 4,861 in the roots, respectively.【Conclusion】GO database comparison showed that there were 43 GO conditions enriched in leaves and 42 GO conditions enriched in roots of sour jujube seedlings. and most of the differentially expressed genes were enriched in the cell membranes of the leaves and roots. KEGG database comparison revealed that the differentially expressed genes were mainly involved in plant-pathogen interaction, MAPK signaling pathway-plant, Plant hormone signal transduction, phenylpropanoid biosynthesis, pentose and glucuronate interconversions, etc.

Key words: sour jujube(Ziziphus acidojujuba); CaCl₂; NaCl stress; transcriptome

王振东, 鲁晓燕, 涂文文, 王晓丽, 何晨晨. 外源CaCl₂缓解NaCl胁迫下酸枣幼苗叶和根转录组测序分析[J]. 新疆农业科学, 2019, 56(6): 1052-1062.

WANG Zhen-Dong, LU Xiao-Yan, TU Wen-Wen, HE Chen-Chen. Transcriptome Sequencing Analysis of Leaf and Root of Sour Jujube Seedlings under NaCl Stress Alleviated by Exogenous CaCl₂[J]. Xinjiang Agricultural Sciences, 2019, 56(6): 1052-1062.

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外源CaCl₂缓解NaCl胁迫下酸枣幼苗叶和根转录组测序分析

Transcriptome Sequencing Analysis of Leaf and Root of Sour Jujube Seedlings under NaCl Stress Alleviated by Exogenous CaCl₂

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