新疆农业科学 ›› 2019, Vol. 56 ›› Issue (4): 678-684.DOI: 10.6048/j.issn.1001-4330.2019.04.010

• 作物遗传育种·种质资源·分子生物学·生理生化 • 上一篇    下一篇

新疆野苹果次生代谢产物对虫害胁迫的响应

梅闯, 闫鹏, 艾沙江·买买提, 朱燕飞, 马凯, 韩立群, 王继勋   

  1. 新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐 830091
  • 收稿日期:2019-01-15 出版日期:2019-04-20 发布日期:2019-07-08
  • 通信作者: 王继勋(1965-),男,安微砀山人,研究员,研究方向为果树资源与育种,(E-mail)ee_wjx@163.com
  • 作者简介:梅闯(1985-),男,四川南充人,助理研究员,研究方向为果树育种与生物技术,(E-mail)meichuangxj@163.com
  • 基金资助:
    新疆维吾尔自治区自然科学基金(2016D01B042)

Response of Secondary Metabolites to Insect Stress in Xinjiang Wild Apple

MEI Chuang, YAN Peng, Aisajan Mamat, ZHU Yan-fei, MA Kai, HAN Li-qun, WANG Ji-xun   

  1. Research Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences / Scientific Observing and Experimental Station of Pomology (Xinjiang), Ministry of Agriculture, Urumqi 830091,China
  • Received:2019-01-15 Online:2019-04-20 Published:2019-07-08
  • Correspondence author: WANG Ji-xun,(1965-),Researcher, Research orientation: Fruit tree resources and Facility cultivation, (E-mail)ee_wjx@163.com

摘要: 【目的】研究新疆野苹果(Malus sieversii)受到虫害胁迫诱导后相关次生代谢物质的变化及其产生规律,为保护利用新疆野苹果资源提供理论依据。【方法】以经过前期鉴定稳定遗传的抗虫株系为材料,利用液相色谱-质谱(LC-MS)联用技术,定性定量测定次生代谢物质含量变化趋势。【结果】在自然条件下,香豆酸含量显著高于抗虫株系,含量分别为2.2×104和0.5×104 μg/mL,差异倍数达到4.4倍。反式肉桂酸、咖啡酸、香草酸、阿魏酸含量变化不大;虫害侵染胁迫条件下,羟基肉桂酰衍生物中的反式肉桂酸含量上升明显,达到极显著水平,含量分别为3.3×104和2.2×106 μg/mL,差异倍数为6.8倍。抗虫株系的咖啡酸含量极显著高于对照株系,提升倍数为3.8,阿魏酸含量也得到显著提升。对照株系榭皮素含量显著高于抗虫株系,达到44.3%,生物碱各组分及苯甲酸、绿原酸含量变化均不显著。【结论】新疆野苹果被苹果小吉丁虫(Agrilusmali Mats.)侵染诱导胁迫后,羟基肉桂酰衍生物等相关次生代谢产物产生积极响应。

关键词: 新疆野苹果; 苹果小吉丁虫; 抗虫性; 次生代谢产物

Abstract: 【Objective】 The aim of the study is to provide a theoretical basis for the effective protection and utilization of Xinjiang wild apple (Malus sieversii) by understanding the changes of secondary metabolites and their generation pattern in the plant under insect stress. 【Method】 The content change trend of secondary metabolites was determined qualitatively and quantitatively by liquid chromatography-mass spectrometry (LC-MS) using insect-resistant strains identified in the previous period as materials. 【Result】 Under natural conditions without pest stress, the content of coumaric acid in the control group was significantly higher than that of the resistant strains, which were 2.2×104 μg/mL versus 0.5×104 μg/mL respectively, with a fold change of 4.4. The contents of trans-cinnamic acid, caffeic acid, vanillic acid and ferulic acid did not change much. Under the insect infection condition, the content of trans-cinnamic acid, one of the hydroxycinnamyl derivatives, increased significantly to a very high level. The figures were 3.3 ×104 μg/mL and 2.2×106 μg/mL in the control and the resistant group with a fold change of 6.8. At the same time, the caffeic acid in the insect-resistant strains was significantly higher or 3.8 times of the control ones. Similar change was observed in ferulic acid content. The control strains were significantly richer in quercetin compared with the resistant ones, reaching 44.3%. And the changes of alkaloids, benzoic acid and chlorogenic acid were not significant. 【Conclusion】 Secondary metabolites such as hydroxycinnamyl derivatives experienced positive responses in Xinjiang wild apple after the invasion of Agrilusmali Mats

Key words: Malus sieversii; Agrilusmali Mats; insect resistance; secondary metabolite


ISSN 1001-4330 CN 65-1097/S
邮发代号:58-18
国外代号:BM3342
主管:新疆农业科学院
主办:新疆农业科学院 新疆农业大学 新疆农学会

出版单位:《新疆农业科学》编辑部
地址:乌鲁木齐市南昌路403号新疆农业科学院
邮编:830091
电话:0991-4502046
E-mail:xjnykx-h@xaas.ac.cn


版权所有 © 《新疆农业科学》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量: 今日访问: 在线人数:
网站
微信公众号
淘宝购买
微店购买